The Ensembl Database
... Using RepeatMasker, we masked all drosophilaspecific repeats from the sequence Using blastx, we searched this sequence against the Swissprot database blastx results indicate our sequence is similar to the Paired-box protein (Pax6) in D. melanogaster ...
... Using RepeatMasker, we masked all drosophilaspecific repeats from the sequence Using blastx, we searched this sequence against the Swissprot database blastx results indicate our sequence is similar to the Paired-box protein (Pax6) in D. melanogaster ...
Measuring Arsenite using E. coli reporter strains
... with specific DNA regions and its ability to react with arsenite. By using genetic engineering an artificial coupling was created between a DNA fragment containing the binding site for ArsR and a DNA fragment with the gene for the reporter protein (eGFP for enhanced Green Fluorescent Protein, Fig. 1 ...
... with specific DNA regions and its ability to react with arsenite. By using genetic engineering an artificial coupling was created between a DNA fragment containing the binding site for ArsR and a DNA fragment with the gene for the reporter protein (eGFP for enhanced Green Fluorescent Protein, Fig. 1 ...
The role of IRES trans-acting factors in regulating translation initiation
... Significant advances have been made in identifying how ITAFs regulate viral IRESs. For example, it is known that PTB regulates the EMCV IRES by stabilizing its threedimensional structure, thus acting as an RNA chaperone [29]. Moreover, research in the viral IRES field has been aided by structural si ...
... Significant advances have been made in identifying how ITAFs regulate viral IRESs. For example, it is known that PTB regulates the EMCV IRES by stabilizing its threedimensional structure, thus acting as an RNA chaperone [29]. Moreover, research in the viral IRES field has been aided by structural si ...
Spinal Muscular Atrophy May 2014
... neurons that control muscle movement is thought to contribute to the muscle weakness experienced by people with SMA-LED. It is unclear why this condition affects only the lower extremities. The adult-onset form of spinal muscular atrophy is caused by a mutation in the VAPB gene. The VAPB gene provid ...
... neurons that control muscle movement is thought to contribute to the muscle weakness experienced by people with SMA-LED. It is unclear why this condition affects only the lower extremities. The adult-onset form of spinal muscular atrophy is caused by a mutation in the VAPB gene. The VAPB gene provid ...
Drug delivery systems based on sugar
... mechanism that has been targeted, and consequently much interest has focused on the proteins involved in RME, such as the mannose binding protein (MBP). In particular, the ASGPR in the liver is a particularly attractive target, not least because of its very high density on hepatocyte surfaces (50,00 ...
... mechanism that has been targeted, and consequently much interest has focused on the proteins involved in RME, such as the mannose binding protein (MBP). In particular, the ASGPR in the liver is a particularly attractive target, not least because of its very high density on hepatocyte surfaces (50,00 ...
Gene Section GMPS (guanine monphosphate synthetase) Atlas of Genetics and Cytogenetics
... nucleotides: amidotransferase that catalyzes the amination of xanthosine 5 prime monophosphate to form GMP in the presence of ATP and glutamine; GTP is also involved in many enzymatic reactions important for cell division. ...
... nucleotides: amidotransferase that catalyzes the amination of xanthosine 5 prime monophosphate to form GMP in the presence of ATP and glutamine; GTP is also involved in many enzymatic reactions important for cell division. ...
Chp 19
... Section 19.2: Protein Synthesis §80S complex - initiation complex binds the 60S subunit §Hydrolysis of GTP bound to eIF2 §eIF5-acts as a guanine nucleotide activating protein §Initiation factors are released from the ribosome ...
... Section 19.2: Protein Synthesis §80S complex - initiation complex binds the 60S subunit §Hydrolysis of GTP bound to eIF2 §eIF5-acts as a guanine nucleotide activating protein §Initiation factors are released from the ribosome ...
Review Article The biosafety of molecular farming in plants
... related wild species have also been documented along this route. For example, King (1996) noted that 50% of wild strawberries growing near a field of cultivated transgenic strawberries contained marker genes from the transgenic population. Similarly, herbicide resistance genes have introgressed from ...
... related wild species have also been documented along this route. For example, King (1996) noted that 50% of wild strawberries growing near a field of cultivated transgenic strawberries contained marker genes from the transgenic population. Similarly, herbicide resistance genes have introgressed from ...
Lecture 19 Membranes 2: Membrane Proteins
... whose functions require protein-protein interactions (collision probability much greater in "corral" of local raft). • Proteins and lipids move in and out of rafts, but on slower time scale (sec rather than µsec), but biochemical processes work on the faster time scale in which rafts are stable clus ...
... whose functions require protein-protein interactions (collision probability much greater in "corral" of local raft). • Proteins and lipids move in and out of rafts, but on slower time scale (sec rather than µsec), but biochemical processes work on the faster time scale in which rafts are stable clus ...
Identification of a GDP-L-fucose: polypeptide fucosyltransferase and
... mentioned above. Detailed NMR study (Mer et al., 1996) showed that the O-fucosylation of the peptide was responsible for an overall decrease of the dynamic fluctuations of the molecule and an increase in stability as monitored by thermal denaturation. It is not yet clear whether the biosynthesis of ...
... mentioned above. Detailed NMR study (Mer et al., 1996) showed that the O-fucosylation of the peptide was responsible for an overall decrease of the dynamic fluctuations of the molecule and an increase in stability as monitored by thermal denaturation. It is not yet clear whether the biosynthesis of ...
RNA Processing in Eukaryotes
... may be involved in gene regulation but are removed from the pre-mRNA during processing. Intron sequences in mRNA do not encode functional proteins. The discovery of introns came as a surprise to researchers in the 1970s who expected that pre-mRNAs would specify protein sequences without further proc ...
... may be involved in gene regulation but are removed from the pre-mRNA during processing. Intron sequences in mRNA do not encode functional proteins. The discovery of introns came as a surprise to researchers in the 1970s who expected that pre-mRNAs would specify protein sequences without further proc ...
minervini
... Europe (EGEE) and China (CNGrid) Grid infrastructures The goals of the EUChinaGRID are: ...
... Europe (EGEE) and China (CNGrid) Grid infrastructures The goals of the EUChinaGRID are: ...
Y41G9a.1 and ciliary defects in osm
... and its corresponding protein polaris in the mouse, osm-5 expression is consistent with this ciliogenic role. osm-5 expression, as revealed by a GFP transcriptional fusion with the endogenous osm-5 promoter, is detected in ciliated sensory neurons. In addition, the OSM-5 protein was found to concent ...
... and its corresponding protein polaris in the mouse, osm-5 expression is consistent with this ciliogenic role. osm-5 expression, as revealed by a GFP transcriptional fusion with the endogenous osm-5 promoter, is detected in ciliated sensory neurons. In addition, the OSM-5 protein was found to concent ...
Auxin Action in a Cell
... added directly to protein extracts prepared from seedlings expressing c-myc-tagged TIR1 (TIR1-myc). A glutathionine-S-transferase fusion protein (GST-IAA7) was added to extracts together with the endogenous auxin IAA or the synthetic auxin 2,4-D. GST-IAA7 was recovered, and the presence of TIR1-myc ...
... added directly to protein extracts prepared from seedlings expressing c-myc-tagged TIR1 (TIR1-myc). A glutathionine-S-transferase fusion protein (GST-IAA7) was added to extracts together with the endogenous auxin IAA or the synthetic auxin 2,4-D. GST-IAA7 was recovered, and the presence of TIR1-myc ...
THE BITHORAX COMPLEX: THE FIRST FIFTY YEARS
... “unequal” crossing over. Interestingly, Wright had predicted that B itself would be a duplication before it was demonstrated cytologically (8). Bridges had earlier called attention to duplication-like structures in the salivary gland chromosomes of wild-type larvae (9). In particular, he interpreted ...
... “unequal” crossing over. Interestingly, Wright had predicted that B itself would be a duplication before it was demonstrated cytologically (8). Bridges had earlier called attention to duplication-like structures in the salivary gland chromosomes of wild-type larvae (9). In particular, he interpreted ...
Genetically engineered gold-binding polypeptides
... via disul de bonds [11– 13], the nature of GBP binding is thought to differ from this well-known thiol linkage and offer a new avenue for protein – gold surface interaction. The GBP sequences are all found to be rich in serine and threonine, and physisorption of these polar side-chains onto gold ma ...
... via disul de bonds [11– 13], the nature of GBP binding is thought to differ from this well-known thiol linkage and offer a new avenue for protein – gold surface interaction. The GBP sequences are all found to be rich in serine and threonine, and physisorption of these polar side-chains onto gold ma ...
Molecular mechanisms of Salmonella invasion
... of Salmonella virulence provided by these studies are believed to be highly relevant to the understanding of the natural infection process [25, 28, 50]. The ability of S. typhimurium to invade ...
... of Salmonella virulence provided by these studies are believed to be highly relevant to the understanding of the natural infection process [25, 28, 50]. The ability of S. typhimurium to invade ...
Chapter 8
... Pre-mRNAs and other nuclear RNAs are collectively known as heterogeneous nuclear RNA (hnRNA). hnRNA is extensively bound to binding proteins, and complexes between hnRNA and protein are called heterogeneous ribonucleoprotein particles (hnRNP). Binding proteins function by preventing hnRNA from formi ...
... Pre-mRNAs and other nuclear RNAs are collectively known as heterogeneous nuclear RNA (hnRNA). hnRNA is extensively bound to binding proteins, and complexes between hnRNA and protein are called heterogeneous ribonucleoprotein particles (hnRNP). Binding proteins function by preventing hnRNA from formi ...
Cofactor requirement of ribosome-inactivating
... Key words: Cofactors, ribosome-inactivating proteins, RNA-/V-glycosidase, up-regulation. ...
... Key words: Cofactors, ribosome-inactivating proteins, RNA-/V-glycosidase, up-regulation. ...
Lung Cancer and the ALK S1206Y Mutation This material will help
... Lung Cancer and the ALK S1206Y Mutation This material will help you understand: • the basics of lung cancer • the role of the ALK gene in lung cancer • if there are any drugs that might work better if you have certain changes in the ALK gene What is lung cancer? Lung cancer is a type of cancer that ...
... Lung Cancer and the ALK S1206Y Mutation This material will help you understand: • the basics of lung cancer • the role of the ALK gene in lung cancer • if there are any drugs that might work better if you have certain changes in the ALK gene What is lung cancer? Lung cancer is a type of cancer that ...
Lung Cancer and the ALK G1269A Mutation This material will help
... Lung Cancer and the ALK G1269A Mutation This material will help you understand: • the basics of lung cancer • the role of the ALK gene in lung cancer • if there are any drugs that might work better if you have certain changes in the ALK gene What is lung cancer? Lung cancer is a type of cancer that ...
... Lung Cancer and the ALK G1269A Mutation This material will help you understand: • the basics of lung cancer • the role of the ALK gene in lung cancer • if there are any drugs that might work better if you have certain changes in the ALK gene What is lung cancer? Lung cancer is a type of cancer that ...
Gel-Filtration Chromatography - DORAS
... performing gel-filtration chromatography, one generally assumes that all of the molecules within a mixture have the same symmetrical shape, so that the order of elution will be one of decreasing molecular weight. Whereas this is an acceptable assumption in most cases, one must bear in mind that the ...
... performing gel-filtration chromatography, one generally assumes that all of the molecules within a mixture have the same symmetrical shape, so that the order of elution will be one of decreasing molecular weight. Whereas this is an acceptable assumption in most cases, one must bear in mind that the ...
Lung Cancer and the ALK L1196M Mutation This material will help
... Lung Cancer and the ALK L1196M Mutation This material will help you understand: • the basics of lung cancer • the role of the ALK gene in lung cancer • if there are any drugs that might work better if you have certain changes in the ALK gene What is lung cancer? Lung cancer is a type of cancer that ...
... Lung Cancer and the ALK L1196M Mutation This material will help you understand: • the basics of lung cancer • the role of the ALK gene in lung cancer • if there are any drugs that might work better if you have certain changes in the ALK gene What is lung cancer? Lung cancer is a type of cancer that ...
Transport of Phosphatidylserine from the Endoplasmic Reticulum to
... with a marker for vacuoles (>65%) but a significant proportion (35%) also co-fractionated with markers for the Golgi complex, leading to the conclusion that Psd2p is distributed in these compartments (12). Further experiments with Psd2p revealed that it contains a putative Golgi retention sequence ( ...
... with a marker for vacuoles (>65%) but a significant proportion (35%) also co-fractionated with markers for the Golgi complex, leading to the conclusion that Psd2p is distributed in these compartments (12). Further experiments with Psd2p revealed that it contains a putative Golgi retention sequence ( ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.