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here - The Nebenführ Lab
here - The Nebenführ Lab

... © Springer-Verlag 2004 ...
Forespore membrane assembly in yeast
Forespore membrane assembly in yeast

... Here, I discuss the molecular mechanisms shared by fission and budding yeast sporulation. In particular, I focus on de novo synthesis of the spore plasma membrane, which is originally constructed as a double membrane called the forespore membrane (FSM), and the role of spindle pole bodies (SPBs) as ...
Early steps in cold sensing by plant cells: the role of actin
Early steps in cold sensing by plant cells: the role of actin

... Previous studies (Murata and Los, 1997) have suggested that the plasma membrane acts as the primary sensor of temperature change through dynamic changes in its physical state. Rigidi®cation of plasma membranes by Pd-catalysed hydrogenation leads to the induction of the cold-inducible gene, desA, enc ...
pig-1_final 121812
pig-1_final 121812

... example, divide to produce a neuronal precursor and a cell that dies, but how this fate asymmetry is generated in these divisions is poorly understood. PIG-1 regulates asymmetric neuroblast divisions that generate apoptotic cells by controlling spindle positioning, myosin distribution and daughter c ...
D:Int Agrophysics -2Cybulskacybulska.vp - Research
D:Int Agrophysics -2Cybulskacybulska.vp - Research

... which constitute more than 90% of their dry mass. Polysaccharides of cell walls are cellulose (15-30% d.m.), pectin (about 30% d.m.) and hemicelluloses (about 25% d.m.) (Taiz and Zeiger, 2002). Spatial structure of bacterial cellulose microfibrils which is produced by Gluconacetobacter xylinus is co ...
ANISOTROPIC EXPANSION OF THE PLANT CELL WALL
ANISOTROPIC EXPANSION OF THE PLANT CELL WALL

... as well as apical cells in some filamentous algae and lower plants, grow by tip growth. In this mode, expansion is confined to one end of the cell, the tip, in contrast with diffuse growth in which growth occurs throughout one or more faces of the cell. For a single tip-growing cell, the stress distri ...
Polarity Control of Spindle Positioning in the C. elegans Embryo
Polarity Control of Spindle Positioning in the C. elegans Embryo

What Does the Microsporidian E. cuniculi Tell Us About the Origin of
What Does the Microsporidian E. cuniculi Tell Us About the Origin of

... S. cerevisiae proteins with proteins of D. melanogaster, C. elegans, A. thaliana, and G. lamblia and those of 44 bacteria and archaea species. We used a blast score of 55 bits. This score was based on our consultation with experts in bioinformatics, as the Giardia database was in contigs only and wa ...
Characterization of chloroplasts in pavement cells of Arabidopsis
Characterization of chloroplasts in pavement cells of Arabidopsis

... Pavement cell chloroplasts (PCC) in Arabidopsis are comparable in size to guard cell chloroplasts but are approximately half the size of mesophyll chloroplasts (MCC). In the jigsaw shaped pavement cells in expanded cotyledons and leaves of Arabidopsis, 9 to 15 PCC are found per cell, while a single ...
Signatures of nitrogen limitation in the elemental composition of the
Signatures of nitrogen limitation in the elemental composition of the

... proteins involved in major metabolic pathways and cellular components is linked to the environmental factors that trigger their activation, we analysed the N content of the proteins involved in the anabolic and catabolic apparatus in four multicellular eukaryotic model organisms. We further investig ...
figure 1 - Proceedings of the Royal Society B
figure 1 - Proceedings of the Royal Society B

... proteins involved in major metabolic pathways and cellular components is linked to the environmental factors that trigger their activation, we analysed the N content of the proteins involved in the anabolic and catabolic apparatus in four multicellular eukaryotic model organisms. We further investig ...
Submembraneous microtubule cytoskeleton: biochemical and
Submembraneous microtubule cytoskeleton: biochemical and

... Different post-translationally modified tubulin, like tyrosinated tubulin (a marker for dynamic microtubules), detyrosinated tubulin, acetylated tubulin, polyglutamylated tubulin, phospho (serine) tubulin and neurone-specific b-III tubulin (all markers for stable microtubules) interact with TRPV1-Ct [ ...
Balancing mitochondrial biogenesis and
Balancing mitochondrial biogenesis and

... NRF2/NFE2L2 is tethered to mitochondria through association with Keap1 and PGAM5 proteins.17 SKN-1 was also found to interact with the outer mitochondrial protein PGAM-5.18 Calcium signalling modulates mitochondrial biogenesis through the calcium/calmodulin-dependent protein kinase II (CaMKII), p38 ...
NO 3
NO 3

... The apoplast (apo, greek= away from the plasma) is the continuous system of water and air filled spaces of the cell wall and is in close contact with the soil medium, the contact often being enhanced by numerous root hairs. Water free space(水分自由空间) and Donnan free space( 杜南自由空间) Cations interact wit ...
Bacterial tail anchors can target to the mitochondrial outer
Bacterial tail anchors can target to the mitochondrial outer

... recent evidence indicates that archaeal clades potentially related to the last eukaryotic ...
Ribosome-tethered molecular chaperones
Ribosome-tethered molecular chaperones

PDF
PDF

... The observations reported in this investigation were made on the indifferent gonad and newly formed ovarian tissue removed from larvae and mature ovarian material excised from adult females of Xenopus laevis. The premetamorphic animals were staged according to the scheme proposed by Nieuwkoop & Fabe ...
Mechanisms Shaping the Membranes of Cellular Organelles
Mechanisms Shaping the Membranes of Cellular Organelles

... Cellular organelles have characteristic morphologies that arise as a result of different local membrane curvatures. A striking example is the endoplasmic reticulum (ER), which consists of ER tubules with high curvature in cross-section, peripheral ER sheets with little curvature except at their edge ...
Acyl-CoA oxidase is imported as a heteropentameric, cofactor
Acyl-CoA oxidase is imported as a heteropentameric, cofactor

... the acquisition and maintenance of the oligomeric conformation by other proteins imported into the peroxisome are assisted by a specialized set of cytosolic chaperones or whether they can occur spontaneously in the cytosol. Furthermore, it is unknown if any peroxisomal protein can preassemble with i ...
The Forces Behind Cell Movement
The Forces Behind Cell Movement

... the forces that govern the crawling of single cells. It also references several websites with animations and movies, given in footnotes, to clarify the processes described. As a cell moves on a substrate (the extracellular matrix if the cell moves inside an organism or a cover slide if it moves outs ...
REVIEW - The Journal of Cell Biology
REVIEW - The Journal of Cell Biology

... accounts for ~ 2 5 - 3 0 % of the total thylakoid membrane proteins, and it consists of at least two integral polypeptides (90) of similar molecular weights (cf. reference 4). Several peripheral membrane proteins including ferredoxin, ferredoxinNADP reductase (cf. reference 19), plastocyanin (53, 61 ...
Full Text  - Molecular Biology and Evolution
Full Text - Molecular Biology and Evolution

... This dramatic reduction in the conservation of membrane proteins is widespread across the entire tree of life, but the effect decreases as cellular or organismal complexity increases. Water-soluble proteins have on average 2.7 times more orthologs than membrane proteins in prokaryotes. The factor de ...
Pectin methylesterases: cell wall enzymes with important roles in
Pectin methylesterases: cell wall enzymes with important roles in

Planar cell polarity signalling regulates cell adhesion properties in
Planar cell polarity signalling regulates cell adhesion properties in

... DFCs were automatically isolated using a FACSAria cell sorting system (BD Biosciences). Cells were then used for single-cell force spectroscopy (CellHesion, JPK Instruments, Berlin, Germany). Using plasma-activated cantilevers (Veeco NP-0, nominal spring constant k60 mN/m), cells were incubated in ...
In cellulo Evaluation of Phototransformation Quantum - HAL
In cellulo Evaluation of Phototransformation Quantum - HAL

... background level, laser power, frametime) adequate for an accurate determination of the phototransformation yields. Under illumination at 561 nm in PBS buffer at pH 7.4, the photobleaching yield of Dendra2 fused to actin was measured to be (2.560.4)61025, whereas the blinking-off yield and thermally ...
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Cytosol



The cytosol or intracellular fluid (ICF) or cytoplasmic matrix is the liquid found inside cells. It is separated into compartments by membranes. For example, the mitochondrial matrix separates the mitochondrion into many compartments.In the eukaryotic cell, the cytosol is within the cell membrane and is part of the cytoplasm, which also comprises the mitochondria, plastids, and other organelles (but not their internal fluids and structures); the cell nucleus is separate. In prokaryotes, most of the chemical reactions of metabolism take place in the cytosol, while a few take place in membranes or in the periplasmic space. In eukaryotes, while many metabolic pathways still occur in the cytosol, others are contained within organelles.The cytosol is a complex mixture of substances dissolved in water. Although water forms the large majority of the cytosol, its structure and properties within cells is not well understood. The concentrations of ions such as sodium and potassium are different in the cytosol than in the extracellular fluid; these differences in ion levels are important in processes such as osmoregulation, cell signaling, and the generation of action potentials in excitable cells such as endocrine, nerve and muscle cells. The cytosol also contains large amounts of macromolecules, which can alter how molecules behave, through macromolecular crowding.Although it was once thought to be a simple solution of molecules, the cytosol has multiple levels of organization. These include concentration gradients of small molecules such as calcium, large complexes of enzymes that act together to carry out metabolic pathways, and protein complexes such as proteasomes and carboxysomes that enclose and separate parts of the cytosol.
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