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459 Resources
... • In mitochondria, the process of cellular respiration combines food molecules with oxygen. This series of chemical reactions releases energy for the cell's activities. • The energy in light is captured and stored in food molecules during the process of photosynthesis. Plants, algae, and some bacter ...
... • In mitochondria, the process of cellular respiration combines food molecules with oxygen. This series of chemical reactions releases energy for the cell's activities. • The energy in light is captured and stored in food molecules during the process of photosynthesis. Plants, algae, and some bacter ...
Modulation of oxidative stress as an anticancer strategy
... stress. Cancer cells have a high metabolic activity and require high levels of ATP to sustain their uncontrolled proliferation and growth. These two aspects result in sustained mitochondrial respiration, which leads to excessive ROS accumulation5. ER stress. The correct folding of proteins in the en ...
... stress. Cancer cells have a high metabolic activity and require high levels of ATP to sustain their uncontrolled proliferation and growth. These two aspects result in sustained mitochondrial respiration, which leads to excessive ROS accumulation5. ER stress. The correct folding of proteins in the en ...
The linkage between cell wall metabolism and fruit
... We focus on tomato in this review because it represents the predominant model for studying fruit ripening,5 it has been employed in studies emphasising the use of transgenes since the 1980s, and probably more is known about the structure and metabolism of cell walls from tomato fruit than those of a ...
... We focus on tomato in this review because it represents the predominant model for studying fruit ripening,5 it has been employed in studies emphasising the use of transgenes since the 1980s, and probably more is known about the structure and metabolism of cell walls from tomato fruit than those of a ...
Changes in endothelial phenotype may explain changes in
... Systemic PMNs were depleted by multiple injections (i.p) of anti-Gr-1 monoclonal antibody (RB6-8C5 clone, 0.1 mg each injection, eBioscience, CA). For early and sustained depletion of PMNs (pre Gr-1), mice were treated with Gr-1 antibody or isotype control rat IgG (eBioscience, CA) every other day, ...
... Systemic PMNs were depleted by multiple injections (i.p) of anti-Gr-1 monoclonal antibody (RB6-8C5 clone, 0.1 mg each injection, eBioscience, CA). For early and sustained depletion of PMNs (pre Gr-1), mice were treated with Gr-1 antibody or isotype control rat IgG (eBioscience, CA) every other day, ...
The nucleolus through the years
... various staining procedures and microscopic techniques than the nuclear chromatin and exhibited a special structural organization (Montgomery 1899, Ruzicka 1899, Busch and Smetana 1970). Actually, based on the results of such methodical approaches, nucleoli appeared somehow different in various cell ...
... various staining procedures and microscopic techniques than the nuclear chromatin and exhibited a special structural organization (Montgomery 1899, Ruzicka 1899, Busch and Smetana 1970). Actually, based on the results of such methodical approaches, nucleoli appeared somehow different in various cell ...
Conditional lethality of a yeast strain expressing human RHOA in place of RHO1 .
... examined after incubation at the restrictive YOC725 cells stop growing after -6 h, but the bud sizes appear to be random. Flow cytometric analysis (data not shown) further confirms that the cells stop growing at all stages of the cell cycle. Since many cells looked fragile under the microscope, we t ...
... examined after incubation at the restrictive YOC725 cells stop growing after -6 h, but the bud sizes appear to be random. Flow cytometric analysis (data not shown) further confirms that the cells stop growing at all stages of the cell cycle. Since many cells looked fragile under the microscope, we t ...
Plant Cell
... 2014; Figure 2). It should be noted that the properties of some of the proteins described above may be different when compared between different plant species, for example, Os-TPC1 and AtTPC1, and that some of the experiments conducted to characterize these properties were performed in vitro. Despit ...
... 2014; Figure 2). It should be noted that the properties of some of the proteins described above may be different when compared between different plant species, for example, Os-TPC1 and AtTPC1, and that some of the experiments conducted to characterize these properties were performed in vitro. Despit ...
Bioactive Compounds from Streptomyces nasri and its Mutants with
... which have proven to be most useful as physiological active drugs like branched glucans of Dictyophora sp. and Symphitum fructans (Franz, 1989). Many interesting areas have been opened, which include their role in cancer therapy (Schizophyllan) and prevention of bacterial and viral diseases (Lentina ...
... which have proven to be most useful as physiological active drugs like branched glucans of Dictyophora sp. and Symphitum fructans (Franz, 1989). Many interesting areas have been opened, which include their role in cancer therapy (Schizophyllan) and prevention of bacterial and viral diseases (Lentina ...
Copyright Information of the Article Published Online
... resistance[4]. Accumulating evidence indicates that a subset of cancer cells with high self-renewal and stemness properties, known as cancer stem cells (CSCs), are the key contributors to chemo-resistance, and are responsible for tumor progression and recurrence after conventional therapy[5]. CD44, ...
... resistance[4]. Accumulating evidence indicates that a subset of cancer cells with high self-renewal and stemness properties, known as cancer stem cells (CSCs), are the key contributors to chemo-resistance, and are responsible for tumor progression and recurrence after conventional therapy[5]. CD44, ...
RalA ACTIVATION ASSAY BIOCHEM KIT
... proteins, RalA and RalB, share 58% identity to these oncoproteins and 85% identity with each other (Chardin and Tavitian 1986). Ral proteins play an important role in diverse cellular processes including endocytosis, exocytosis, oncogenesis and the regulation of transcription and cell morphology (Fe ...
... proteins, RalA and RalB, share 58% identity to these oncoproteins and 85% identity with each other (Chardin and Tavitian 1986). Ral proteins play an important role in diverse cellular processes including endocytosis, exocytosis, oncogenesis and the regulation of transcription and cell morphology (Fe ...
PPT - Altogen Biosystems
... Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent 12-24 hours after transfection If the viability of H9c2 cells being transfected is affected at 16 - 24 hours post-transfection, the level of cytotoxicity can be ...
... Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent 12-24 hours after transfection If the viability of H9c2 cells being transfected is affected at 16 - 24 hours post-transfection, the level of cytotoxicity can be ...
Full-Text PDF
... population of T cells in the peripheral immune system comprising >90% of the total T cells. These cells can give rise to two distinct subsets referred to as CD4+ Thelper (Th ) cells which help B cells to produce antibodies and CD8+ cytotoxic T cells (CTLs) which help to recognize and destroy virus i ...
... population of T cells in the peripheral immune system comprising >90% of the total T cells. These cells can give rise to two distinct subsets referred to as CD4+ Thelper (Th ) cells which help B cells to produce antibodies and CD8+ cytotoxic T cells (CTLs) which help to recognize and destroy virus i ...
Drac1 and Crumbs in amnioserosa morphogenesis
... large apical surface area and are elongated perpendicular to the anterior-posterior (A-P) axis of the embryo (Fig. 1A). There are no major accumulations of myosin (Fig. 1B) or F-actin (data not shown) in the amnioserosa at this time. As dorsal closure proceeds, cells at the anterior and posterior en ...
... large apical surface area and are elongated perpendicular to the anterior-posterior (A-P) axis of the embryo (Fig. 1A). There are no major accumulations of myosin (Fig. 1B) or F-actin (data not shown) in the amnioserosa at this time. As dorsal closure proceeds, cells at the anterior and posterior en ...
p53 Pathway in Renal Cell Carcinoma Is Repressed by a Dominant
... HeLa (cervical carcinoma), A293 (embryonic kidney epithelial cells transRenal cell carcinoma (RCC) accounts for approximately 85% of all formed with DNA of adenovirus type 5), and LNCaP (prostate adenocarcikidney cancers. Approximately 30,000 patients in the United States noma)] were purchased from ...
... HeLa (cervical carcinoma), A293 (embryonic kidney epithelial cells transRenal cell carcinoma (RCC) accounts for approximately 85% of all formed with DNA of adenovirus type 5), and LNCaP (prostate adenocarcikidney cancers. Approximately 30,000 patients in the United States noma)] were purchased from ...
Different Roles for Simple-Cell and Complex
... al. (2001). Connections onto and from inhibitory cells may also express use-dependent changes (Gupta et al., 2000), but these have been omitted for simplicity. As described previously (Troyer et al., 1998), we defined the total synaptic strength of a given type received by a cell by the following: ( ...
... al. (2001). Connections onto and from inhibitory cells may also express use-dependent changes (Gupta et al., 2000), but these have been omitted for simplicity. As described previously (Troyer et al., 1998), we defined the total synaptic strength of a given type received by a cell by the following: ( ...
+Tec - Blood Journal
... cells. This peptide could not be detected in the lysate of 3T3 cells that do not express the tec message endogenously (data not shown). Therefore, we conclude that Tec type IV is a peptide of 70 kD in vivo. A peptide of approximately 80 kD observed in Fig 3, lane 32D, is of the nonspecific binding o ...
... cells. This peptide could not be detected in the lysate of 3T3 cells that do not express the tec message endogenously (data not shown). Therefore, we conclude that Tec type IV is a peptide of 70 kD in vivo. A peptide of approximately 80 kD observed in Fig 3, lane 32D, is of the nonspecific binding o ...
Plant Oligosaccharides – Outsiders among Elicitors?
... effect is not due to the presence of non-carbohydrate contaminants in the fractions, which are hard to avoid. These are the reasons that the data on the extraction of oligosaccharides directly from plant tissue are very limited (table). Studies of the team headed by O. A. Zabotina and A. I. Zabotin ...
... effect is not due to the presence of non-carbohydrate contaminants in the fractions, which are hard to avoid. These are the reasons that the data on the extraction of oligosaccharides directly from plant tissue are very limited (table). Studies of the team headed by O. A. Zabotina and A. I. Zabotin ...
Cooperative organization of bacterial colonies: from genotype to
... characteristics are shown. The first is best characterized by its branching pattern. In the second, the branches are much thinner and all have a twist with the same handedness, while in the third every branch has a leading droplet consisting of many bacteria at its tip. Microscopic observations (des ...
... characteristics are shown. The first is best characterized by its branching pattern. In the second, the branches are much thinner and all have a twist with the same handedness, while in the third every branch has a leading droplet consisting of many bacteria at its tip. Microscopic observations (des ...
Chemical Induction of Hsp70 Reduces -Synuclein
... dx.doi.org/10.1021/cb400017h | ACS Chem. Biol. XXXX, XXX, XXX−XXX ...
... dx.doi.org/10.1021/cb400017h | ACS Chem. Biol. XXXX, XXX, XXX−XXX ...
p.1 MicroRNA miR-144 modulates oxidative stress
... exposed and oxidized, alterations in globin conformation can occur, heme can dissociate from globin, and oxidized membrane and cytoskeletal proteins can become sites for hemichrome binding,23 — all of which can lead to hemolysis and are often seen in SCD erythrocytes.19,24,25 In this report, we have ...
... exposed and oxidized, alterations in globin conformation can occur, heme can dissociate from globin, and oxidized membrane and cytoskeletal proteins can become sites for hemichrome binding,23 — all of which can lead to hemolysis and are often seen in SCD erythrocytes.19,24,25 In this report, we have ...
Herpes simplex virus type 1 alters transcript levels of tumor
... in Ophthalmic and Vision Research and with the National Institutes of Health Guidelines on the Use of Animals in Research. Retinal Glial Cell Cultures Primary retinal glial cell cultures were established using our published techniques,21 which are a modification of the methods of Politi et al.30 Cel ...
... in Ophthalmic and Vision Research and with the National Institutes of Health Guidelines on the Use of Animals in Research. Retinal Glial Cell Cultures Primary retinal glial cell cultures were established using our published techniques,21 which are a modification of the methods of Politi et al.30 Cel ...
Lysosomes and Fas-mediated liver cell death
... pathway of apoptosis by contributing to the permeabilization of the outer mitochondrial membrane, perhaps as a result of Bid cleavage by these enzymes [2]. They could also trigger the cytosolic pathway, by activating effector caspases. However, several caspases, caspase 3 for example, seem to be poo ...
... pathway of apoptosis by contributing to the permeabilization of the outer mitochondrial membrane, perhaps as a result of Bid cleavage by these enzymes [2]. They could also trigger the cytosolic pathway, by activating effector caspases. However, several caspases, caspase 3 for example, seem to be poo ...
Archenteron precursor cells can organize secondary axial structures
... gut tissue or (3) a combination of such signals could be involved (these possibilities are summarized in Fig. 1B). In order to provide experimental support for one of these alternative mechanisms, we have used a chimeric embryo approach to implant gut progenitors in ectopic locations to assess their ...
... gut tissue or (3) a combination of such signals could be involved (these possibilities are summarized in Fig. 1B). In order to provide experimental support for one of these alternative mechanisms, we have used a chimeric embryo approach to implant gut progenitors in ectopic locations to assess their ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.