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Fluorescent properties of c-type cytochromes
... biofilm with fibre optics. There may be practical applications for this approach, not only in the analysis of Geobacter populations in environments, such as the subsurface or on energy-harvesting electrodes, in which they are abundant (Lovley et al., 2004; Lovley, 2006), but also for other microbes ...
... biofilm with fibre optics. There may be practical applications for this approach, not only in the analysis of Geobacter populations in environments, such as the subsurface or on energy-harvesting electrodes, in which they are abundant (Lovley et al., 2004; Lovley, 2006), but also for other microbes ...
Ultrastructural studies of t /t mouse embryos
... 2-cell stage by the presence of nuclear lipid droplets and excessive cytoplasmic lipid. In addition, both homozygous mutant embryos often contain binucleate cells, especially in the later cleavage stages. Usually there is only one binucleate cell per embryo, but embryos of both genotypes may have as ...
... 2-cell stage by the presence of nuclear lipid droplets and excessive cytoplasmic lipid. In addition, both homozygous mutant embryos often contain binucleate cells, especially in the later cleavage stages. Usually there is only one binucleate cell per embryo, but embryos of both genotypes may have as ...
Sucrose Cleavage Pathways in Aspen Wood
... Following this, UDP-glucose (UDP-Glc) is formed from Glc-1-P by UDP-Glc pyrophosporylase (UGPase). Suc-6-P synthase (SPS) produces Suc-P using one molecule of both UDP-Glc and Fru-6-P. Finally, Suc is formed by sucrose phosphate phosphatase which removes the phosphate from Suc-P. In most investigate ...
... Following this, UDP-glucose (UDP-Glc) is formed from Glc-1-P by UDP-Glc pyrophosporylase (UGPase). Suc-6-P synthase (SPS) produces Suc-P using one molecule of both UDP-Glc and Fru-6-P. Finally, Suc is formed by sucrose phosphate phosphatase which removes the phosphate from Suc-P. In most investigate ...
Feasibility of using GFP‐expressing Escherichia coli, coupled with
... tailed grazing experiment with the ciliate T. pyriformis. Each prey type was inoculated into six £asks containing 60 ml Chalkley's medium, to give an initial concentration of 5U107 particles ml31 , in excess of indigenous bacteria (1.25U106 cells ml31 ). All £asks were incubated at 20³C and the RF m ...
... tailed grazing experiment with the ciliate T. pyriformis. Each prey type was inoculated into six £asks containing 60 ml Chalkley's medium, to give an initial concentration of 5U107 particles ml31 , in excess of indigenous bacteria (1.25U106 cells ml31 ). All £asks were incubated at 20³C and the RF m ...
pET System Manual
... Expression). While ampicillin resistance is commonly used for selection in a variety of cloning vectors, kanamycin resistance may be preferable under certain conditions, such as for protein expression in labs requiring GMP standards and when subcloning target genes from other ampicillin-resistant ve ...
... Expression). While ampicillin resistance is commonly used for selection in a variety of cloning vectors, kanamycin resistance may be preferable under certain conditions, such as for protein expression in labs requiring GMP standards and when subcloning target genes from other ampicillin-resistant ve ...
leishmania spp.: completely defined medium without serum and
... of serum-supplemented media for the continuous in vitro cultivation of promastigote forms of various Leishmania species. Typical promastigote morphology was observed in Giemsa-stained smears, regardless of the strain analyzed. Electrophoretic analysis showed that the proteinase patterns of asericall ...
... of serum-supplemented media for the continuous in vitro cultivation of promastigote forms of various Leishmania species. Typical promastigote morphology was observed in Giemsa-stained smears, regardless of the strain analyzed. Electrophoretic analysis showed that the proteinase patterns of asericall ...
Effect of acetic acid on growth of Aspergillus niger
... molecular weight RNA, which did not appear in the control (Fig. 5). Moreover, at 5 hr incubation after replacement into the acetate medium, a large breakdown of ribosomal RNA occurred and the low molecular weight RNA fraction enlarged (Fig. 5). Total DNA in the mycelia was constant during incubation ...
... molecular weight RNA, which did not appear in the control (Fig. 5). Moreover, at 5 hr incubation after replacement into the acetate medium, a large breakdown of ribosomal RNA occurred and the low molecular weight RNA fraction enlarged (Fig. 5). Total DNA in the mycelia was constant during incubation ...
STEROIDOGENESIS IN THECA CELLS OF CHICKEN FOLLICLES
... steroidogenesis by theca interna cells. They reported that in the turkey, the production of P4 and androgen in theca interna cells was stimulated by oLH in the smaller F5 but not in the larger F1 and also not by porcine FSH at the concentration of 1,000 ng/ml in both F1 and F5. Although the distinct ...
... steroidogenesis by theca interna cells. They reported that in the turkey, the production of P4 and androgen in theca interna cells was stimulated by oLH in the smaller F5 but not in the larger F1 and also not by porcine FSH at the concentration of 1,000 ng/ml in both F1 and F5. Although the distinct ...
RNA Processing Bodies, Peroxisomes, Golgi Bodies, Mitochondria
... Golgi apparatus, peroxisomes, mitochondria, plastids, and endoplasmic reticulum (ER) (Boevink et al. 1998, Nebenführ et al. 1999, Mathur et al. 2002, Van Gestel et al. 2002, Ueda et al. 2010). Interestingly, although actin filaments sustain long-distance transport of plant organelles, it has recent ...
... Golgi apparatus, peroxisomes, mitochondria, plastids, and endoplasmic reticulum (ER) (Boevink et al. 1998, Nebenführ et al. 1999, Mathur et al. 2002, Van Gestel et al. 2002, Ueda et al. 2010). Interestingly, although actin filaments sustain long-distance transport of plant organelles, it has recent ...
Varicella-Zoster Virus-Infected Human Sensory Neurons Are
... Pharmingen). Positive control cells were treated with 5% ethanol for 5 min at 37°C. A total of 5 l of annexin V-FITC and 2 l of propidium iodide (PI; Pharmingen) were added to the positive control cells and test cells, followed by incubation at room temperature in the dark for 15 min. To control f ...
... Pharmingen). Positive control cells were treated with 5% ethanol for 5 min at 37°C. A total of 5 l of annexin V-FITC and 2 l of propidium iodide (PI; Pharmingen) were added to the positive control cells and test cells, followed by incubation at room temperature in the dark for 15 min. To control f ...
Lineage-specific stem cells, signals and asymmetries
... KEY WORDS: Asymmetric division, bHLH proteins, Epigenetic regulation of differentiation, Lineage-specific stem cells, Peptide signaling, Stomata ...
... KEY WORDS: Asymmetric division, bHLH proteins, Epigenetic regulation of differentiation, Lineage-specific stem cells, Peptide signaling, Stomata ...
Stat3 and MMP7 Contribute to Pancreatic Ductal Adenocarcinoma
... pancreatic Stat3 deletion. Further study suggested that Stat3 is required to support persistent ductal cell proliferation of Kras-driven metaplastic ductal cells and PanIN of caerulin-treated KRASG12D mice. The expression level of inflammation-associated cytokine is also noted to be lower Kras-drive ...
... pancreatic Stat3 deletion. Further study suggested that Stat3 is required to support persistent ductal cell proliferation of Kras-driven metaplastic ductal cells and PanIN of caerulin-treated KRASG12D mice. The expression level of inflammation-associated cytokine is also noted to be lower Kras-drive ...
distribution of microtubules in the golgi apparatus of euglena gracilis
... 1973), and alter or position dictyosome structure (Mollenhauer, 1965; Mollenhauer & Morre, 1972), they must play a significant role in Golgi apparatus function. The principal deterrent to understanding the intercisternal region is that its components cannot be seen in most electron-microscopical pre ...
... 1973), and alter or position dictyosome structure (Mollenhauer, 1965; Mollenhauer & Morre, 1972), they must play a significant role in Golgi apparatus function. The principal deterrent to understanding the intercisternal region is that its components cannot be seen in most electron-microscopical pre ...
Vol 1. Oral presentations. 6th International PGPR workshop
... for stabilization of mass culture of marine microalgae used as a feed for marine organisms, all with promising results. This aspect of the effect of PGPB on water microorganisms is currently in its infancy. We propose that co-immobilization of microalgae and plant growth-promoting bacteria are an ef ...
... for stabilization of mass culture of marine microalgae used as a feed for marine organisms, all with promising results. This aspect of the effect of PGPB on water microorganisms is currently in its infancy. We propose that co-immobilization of microalgae and plant growth-promoting bacteria are an ef ...
Characterization of Human Corneal Epithelial Cell Model As a
... In the past decade, several in vitro corneal permeability models that can substitute for the isolated cornea to predict ocular absorption and facilitate ophthalmic drug discovery have been developed (Reichl and Becker, 2008). These models evolved from primary cultures using rabbit corneas and immort ...
... In the past decade, several in vitro corneal permeability models that can substitute for the isolated cornea to predict ocular absorption and facilitate ophthalmic drug discovery have been developed (Reichl and Becker, 2008). These models evolved from primary cultures using rabbit corneas and immort ...
proofs oofs proofs proof
... Dialysis treats the major symptoms of kidney failure but it does not cure kidney failure; it simply treats some of the major symptoms. Because of the time required for haemodialysis, this life-saving treatment may negatively affect a patient’s family life and working life; it can limit social life a ...
... Dialysis treats the major symptoms of kidney failure but it does not cure kidney failure; it simply treats some of the major symptoms. Because of the time required for haemodialysis, this life-saving treatment may negatively affect a patient’s family life and working life; it can limit social life a ...
animal phyla - Sakshieducation.com
... a) Organ-system level of body organization b) Bilateral symmetry c) Triploblastic d) Metameric segmentation Neural system in annelid consists of paired ganglia connected by lateral nerves to nerves cord. The nerve cord is/are a) Double, ventral and solid b) Double, dorsal and hollow c) Single, ventr ...
... a) Organ-system level of body organization b) Bilateral symmetry c) Triploblastic d) Metameric segmentation Neural system in annelid consists of paired ganglia connected by lateral nerves to nerves cord. The nerve cord is/are a) Double, ventral and solid b) Double, dorsal and hollow c) Single, ventr ...
Document
... cardiac jelly and its swelling at the atrioventricular canal (AVC) [78, 141]. These data are supported by computer models indicating that the later stages of cardiac looping requires forces external to the primitive heart tube [119]. Further support comes from physical simulation experiments based o ...
... cardiac jelly and its swelling at the atrioventricular canal (AVC) [78, 141]. These data are supported by computer models indicating that the later stages of cardiac looping requires forces external to the primitive heart tube [119]. Further support comes from physical simulation experiments based o ...
Regular-spiking cells in the presubiculum are hyperexcitable in a rat
... 2014; doi:10.1152/jn.00406.2014.—Temporal lobe epilepsy (TLE) is the most common form of adult epilepsy, characterized by recurrent seizures originating in the temporal lobes. Here, we examine TLErelated changes in the presubiculum (PrS), a less-studied parahippocampal structure that both receives i ...
... 2014; doi:10.1152/jn.00406.2014.—Temporal lobe epilepsy (TLE) is the most common form of adult epilepsy, characterized by recurrent seizures originating in the temporal lobes. Here, we examine TLErelated changes in the presubiculum (PrS), a less-studied parahippocampal structure that both receives i ...
Precipitation of calcium, magnesium, strontium and barium in tissues
... calcium-bearing minerals comprise about 50% of known biominerals [1]. Calcium is an essential plant macronutrient and signalling compound, and it performs many fundamental functions in cellular metabolism [7,8]. The formation of calcium crystals is common throughout the plant kingdom, and the most w ...
... calcium-bearing minerals comprise about 50% of known biominerals [1]. Calcium is an essential plant macronutrient and signalling compound, and it performs many fundamental functions in cellular metabolism [7,8]. The formation of calcium crystals is common throughout the plant kingdom, and the most w ...
Differential expression of soluble - Tubitak Journals
... PPa5 were differentially changed in response to glucose, sucrose, or 3-O-methlyglucose (Figure 2). Interestingly, PPa3 transcript level was induced in response to 3-O-methlyglucose in both cell culture (Figure 1) and in planta (Figure 2) experiments. The level of expression of PPa6 (the plastidial i ...
... PPa5 were differentially changed in response to glucose, sucrose, or 3-O-methlyglucose (Figure 2). Interestingly, PPa3 transcript level was induced in response to 3-O-methlyglucose in both cell culture (Figure 1) and in planta (Figure 2) experiments. The level of expression of PPa6 (the plastidial i ...
Circulation is established in a stepwise pattern in the mammalian
... within the yolk sac. In this figure, the yolk sac was photographed with the embryo proper inside, except for panels D and I, where the embryo proper was dissected free of amnion and yolk sac. (Top row) Blood flow was monitored by the redistribution of yolk sac–derived primitive erythroblasts detecte ...
... within the yolk sac. In this figure, the yolk sac was photographed with the embryo proper inside, except for panels D and I, where the embryo proper was dissected free of amnion and yolk sac. (Top row) Blood flow was monitored by the redistribution of yolk sac–derived primitive erythroblasts detecte ...
A Putative Role for Natriuretic Peptides in Fish Osmoregulation
... ANP inhibition of salt uptake in the seawater teleost intestine is somewhat more difficult to rationalize. It would certainly decrease the salt loading of these hypotonic fish, but it is the only way that ingested water (critical for water balance) can be moved from the lumen across the intestinal e ...
... ANP inhibition of salt uptake in the seawater teleost intestine is somewhat more difficult to rationalize. It would certainly decrease the salt loading of these hypotonic fish, but it is the only way that ingested water (critical for water balance) can be moved from the lumen across the intestinal e ...
Bone physiology
... Fig. 1-81 illustrates an osteon with osteocytes (OC) residing in osteocyte lacunae in the lamellar bone. The osteocytes connect via canaliculi (can) which contain cytoplasmatic projections of the osteocytes. A Haversian canal (HC) is seen in the middle of the osteon. Fig. 1-82 illustrates an area ...
... Fig. 1-81 illustrates an osteon with osteocytes (OC) residing in osteocyte lacunae in the lamellar bone. The osteocytes connect via canaliculi (can) which contain cytoplasmatic projections of the osteocytes. A Haversian canal (HC) is seen in the middle of the osteon. Fig. 1-82 illustrates an area ...
RNA Processing Bodies, Peroxisomes, Golgi
... Golgi apparatus, peroxisomes, mitochondria, plastids, and endoplasmic reticulum (ER) (Boevink et al. 1998, Nebenführ et al. 1999, Mathur et al. 2002, Van Gestel et al. 2002, Ueda et al. 2010). Interestingly, although actin filaments sustain long-distance transport of plant organelles, it has recent ...
... Golgi apparatus, peroxisomes, mitochondria, plastids, and endoplasmic reticulum (ER) (Boevink et al. 1998, Nebenführ et al. 1999, Mathur et al. 2002, Van Gestel et al. 2002, Ueda et al. 2010). Interestingly, although actin filaments sustain long-distance transport of plant organelles, it has recent ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.