central spindle and contractile ring for cytokinesis encodes a kinesin
... the cells of the embryo shown in this field are defined using an antibody against a-spectrin, a membrane-bound structural protein. It can be seen that the cell membrane does not invaginate during telophase in pav embryos as it does in wild type (Fig. 1A). Despite this failure of cytokinesis, nuclei ...
... the cells of the embryo shown in this field are defined using an antibody against a-spectrin, a membrane-bound structural protein. It can be seen that the cell membrane does not invaginate during telophase in pav embryos as it does in wild type (Fig. 1A). Despite this failure of cytokinesis, nuclei ...
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... conditions at 28.5°C (Westerfield, 2000). To inhibit pigmentation, embryos were incubated in 0.2 mM phenylthiourea. The arnt2m1055 mutant allele (TU allele number t30256) was isolated from an ENU mutagenesis screen (www.zf-models.org). Other strains used are arnt2hi2639c (Golling et al., 2002) and o ...
... conditions at 28.5°C (Westerfield, 2000). To inhibit pigmentation, embryos were incubated in 0.2 mM phenylthiourea. The arnt2m1055 mutant allele (TU allele number t30256) was isolated from an ENU mutagenesis screen (www.zf-models.org). Other strains used are arnt2hi2639c (Golling et al., 2002) and o ...
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... is the name of a particular structure of ectodermal cells which increase in thickness and become pseudostratified on the tip of the limb bud. Generally, cell and tissue interactions may be mediated by chemical messengers (McMahon, 1974). Among the chemical transmitters acting during early morphogene ...
... is the name of a particular structure of ectodermal cells which increase in thickness and become pseudostratified on the tip of the limb bud. Generally, cell and tissue interactions may be mediated by chemical messengers (McMahon, 1974). Among the chemical transmitters acting during early morphogene ...
Roles of CDK and DDK in Genome Duplication and
... linear order of nuclei in the ascus [70]. These observations suggest that higher levels of DDK and related kinase activities are important for the execution of meiotic recombination and chromosome segregation. Therefore, although the requirements for CDK and DDK during meiosis are more complex than ...
... linear order of nuclei in the ascus [70]. These observations suggest that higher levels of DDK and related kinase activities are important for the execution of meiotic recombination and chromosome segregation. Therefore, although the requirements for CDK and DDK during meiosis are more complex than ...
Human red blood cells-1 - Science Publishing Group
... group AB [12]. We understand that while there are more than 200 minor blood groups today, they occur relatively rarely. The ABO system is still, 100 years later, the primary instrument for determining blood compatibility [13,14,15]. However, citrate anticoagulant was developed to prevent clotting an ...
... group AB [12]. We understand that while there are more than 200 minor blood groups today, they occur relatively rarely. The ABO system is still, 100 years later, the primary instrument for determining blood compatibility [13,14,15]. However, citrate anticoagulant was developed to prevent clotting an ...
effects of light on photosynthesis, grazing, and population dynamics
... was designed to investigate any long-term influence of kleptoplastidy on growth and survival. Pfiesteria piscicida was kept without feeding for 7 days at a constant illumination of 150 mol photonsm2s1 in a 750-mL culture flask, and the culture was then split into six 75-mL culture flasks. Three ...
... was designed to investigate any long-term influence of kleptoplastidy on growth and survival. Pfiesteria piscicida was kept without feeding for 7 days at a constant illumination of 150 mol photonsm2s1 in a 750-mL culture flask, and the culture was then split into six 75-mL culture flasks. Three ...
1 METT-10, A Putative Methyltransferase, Inhibits Germ
... promote the proliferative fate (MUMM and KOPAN 2000). Thus, loss of glp-1 function results in the premature differentiation and meiotic entry of all germ cells (AUSTIN and KIMBLE 1987), while constitutive activation of glp-1 causes germ line tumors (BERRY et al. 1997). Although glp-1 signaling is a ...
... promote the proliferative fate (MUMM and KOPAN 2000). Thus, loss of glp-1 function results in the premature differentiation and meiotic entry of all germ cells (AUSTIN and KIMBLE 1987), while constitutive activation of glp-1 causes germ line tumors (BERRY et al. 1997). Although glp-1 signaling is a ...
The Cell Membrane
... Key Note Things tend to even out, unless something—like a cell membrane— prevents this from happening. Across a freely permeable or water permeable membrane, diffusion and osmosis will ...
... Key Note Things tend to even out, unless something—like a cell membrane— prevents this from happening. Across a freely permeable or water permeable membrane, diffusion and osmosis will ...
Co-ordination of signalling elements in guard cell ion
... exhibit roughly similar single channel conductance. In fact it has been suggested that the same channel protein may account for both gating modes (Blatt and Thiel, 1993). Indeed, changes in anion current kinetics are known to occur in response to ATP depletion ( Thomine et al., 1995), ABA stimulatio ...
... exhibit roughly similar single channel conductance. In fact it has been suggested that the same channel protein may account for both gating modes (Blatt and Thiel, 1993). Indeed, changes in anion current kinetics are known to occur in response to ATP depletion ( Thomine et al., 1995), ABA stimulatio ...
Biosorption of copper from aqueous environments by Micrococcus
... A core-shell electrospinning technique was used for immobilization of the bacteria. The system allows for fabrication of microtubes in an electrostatic field. Two separate syringes continuously ejected solutions from a spinneret with two coaxial capillaries under high voltage. The internal capillary ...
... A core-shell electrospinning technique was used for immobilization of the bacteria. The system allows for fabrication of microtubes in an electrostatic field. Two separate syringes continuously ejected solutions from a spinneret with two coaxial capillaries under high voltage. The internal capillary ...
Perturbation of Polyamine Catabolism Can Strongly Affect Root
... cell types. Thus it is evident that both PA anabolism and catabolism through either cell autonomous and nonautonomous pathways may mediate physiological events associated with PA-induced regulation of growth and development as well as induction of PCD involved in tissue differentiation or defense re ...
... cell types. Thus it is evident that both PA anabolism and catabolism through either cell autonomous and nonautonomous pathways may mediate physiological events associated with PA-induced regulation of growth and development as well as induction of PCD involved in tissue differentiation or defense re ...
Cagnac, O., Leterrier, M., Yeager, M. and Blumwald, E. (2007).
... was generated by replacing the YNL321w ORF with the kanMX6 gene by PCR-based gene deletion method (20) using the following primers: YNL31w-F1 (5⬘-AAGTGAAATAACTGCTAGCTAGAAGAGCGGTAAGCAGCACGGATCCCCGGGTTAATTAA-3⬘) and YNL31w-R1 (5⬘-AAAATTGGTAGGTATCCAGGTGAAAAGCGGGGAC AGTTGCGAATCGCTCGTTTAAAC-3⬘). The dele ...
... was generated by replacing the YNL321w ORF with the kanMX6 gene by PCR-based gene deletion method (20) using the following primers: YNL31w-F1 (5⬘-AAGTGAAATAACTGCTAGCTAGAAGAGCGGTAAGCAGCACGGATCCCCGGGTTAATTAA-3⬘) and YNL31w-R1 (5⬘-AAAATTGGTAGGTATCCAGGTGAAAAGCGGGGAC AGTTGCGAATCGCTCGTTTAAAC-3⬘). The dele ...
Regulation of tubulin heterodimer partitioning during interphase and
... first required for segregation of duplicated chromosomes, and has subsequently evolved to facilitate complex cell shapes and evolution of multi-cellular organisms. The cytoskeleton encompasses microtubules, intermediate filaments and actin filaments (also known as microfilaments), which are polymers ...
... first required for segregation of duplicated chromosomes, and has subsequently evolved to facilitate complex cell shapes and evolution of multi-cellular organisms. The cytoskeleton encompasses microtubules, intermediate filaments and actin filaments (also known as microfilaments), which are polymers ...
Differentiation-associated changes in CD44 isoform expression
... and were performed with the cells at a concentration of107/mL. Cells were first incubated with HFN containing 5% human serum (HS) at room temperature to block Fc receptors, then washed twice with HFN, and incubated with an anti-CD44 MoAb (3C12, 2G1) or the IgGl control MoAb for 30 minutes at 4"C, fo ...
... and were performed with the cells at a concentration of107/mL. Cells were first incubated with HFN containing 5% human serum (HS) at room temperature to block Fc receptors, then washed twice with HFN, and incubated with an anti-CD44 MoAb (3C12, 2G1) or the IgGl control MoAb for 30 minutes at 4"C, fo ...
Expression of Multiple Artificial MicroRNAs from a Chicken miRNA126-Based Lentiviral Vector
... Different strategies have been investigated to express multiple artificial miRNAs from the same lentiviral vector. Zhou et al reported that two tandem copies of the miR30-based cassette can be expressed in a single transcript driven by a Pol II promoter [15,16]. Subsequently, Sun et al showed that a ...
... Different strategies have been investigated to express multiple artificial miRNAs from the same lentiviral vector. Zhou et al reported that two tandem copies of the miR30-based cassette can be expressed in a single transcript driven by a Pol II promoter [15,16]. Subsequently, Sun et al showed that a ...
A Cellular Adventure Reader`s Theater Characters: 1. Teacher 2
... Jenny: Eukaryote not eureka. Eukaryote cells are cells that have a nucleus and organelles surrounded by a membrane. Joe: Oh, I get it. Teacher: Look through your microscope at the slides of animal cells. Jenny: Do we need to draw the cell? Teacher: Good question, Jenny. Yes, you must draw the cell, ...
... Jenny: Eukaryote not eureka. Eukaryote cells are cells that have a nucleus and organelles surrounded by a membrane. Joe: Oh, I get it. Teacher: Look through your microscope at the slides of animal cells. Jenny: Do we need to draw the cell? Teacher: Good question, Jenny. Yes, you must draw the cell, ...
System approaches to study root hairs as a single cell plant model
... specific cells. Hence, our laboratory undertook a similar study to specifically examine the phosphoproteome of soybean root hair cells subsequent to rhizobial inoculation (Nguyen et al., 2012). Again, this is an ideal cell type since it is the site of the initial interaction and penetration of the p ...
... specific cells. Hence, our laboratory undertook a similar study to specifically examine the phosphoproteome of soybean root hair cells subsequent to rhizobial inoculation (Nguyen et al., 2012). Again, this is an ideal cell type since it is the site of the initial interaction and penetration of the p ...
Molecular Plant-Microbe Interactions
... level as PgyBR1 in soybean (data not shown) and was used as the wild-type strain for all our experiments. In order to characterize the functions of HopZ1 alleles in soybean, we constructed a T3SS knock-out mutant PgyBR1Rif:: hrcJΩnptII, which completely lost its ability to multiply in soybean, confi ...
... level as PgyBR1 in soybean (data not shown) and was used as the wild-type strain for all our experiments. In order to characterize the functions of HopZ1 alleles in soybean, we constructed a T3SS knock-out mutant PgyBR1Rif:: hrcJΩnptII, which completely lost its ability to multiply in soybean, confi ...
Oriented cell motility and division underlie early limb bud
... embryos (Hadjantonakis and Papaioannou, 2004) were used because localisation of the H2B-GFP fusion reporter to chromatin facilitates the visualisation of nuclei during all phases of the cell cycle, such that cells can be segmented and individually tracked in four dimensions (three-dimensional time-l ...
... embryos (Hadjantonakis and Papaioannou, 2004) were used because localisation of the H2B-GFP fusion reporter to chromatin facilitates the visualisation of nuclei during all phases of the cell cycle, such that cells can be segmented and individually tracked in four dimensions (three-dimensional time-l ...
Canadian Journal of Microbiology 47
... Transmission electron microscopy Intact beads were first fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.2) for 2 h at 20 ± 2°C, rinsed, and then postfixed in 1% osmium-tetraoxide in the same buffer for 1 h at the same temperature. The beads were then rinsed with distilled water and ...
... Transmission electron microscopy Intact beads were first fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.2) for 2 h at 20 ± 2°C, rinsed, and then postfixed in 1% osmium-tetraoxide in the same buffer for 1 h at the same temperature. The beads were then rinsed with distilled water and ...
Morphine Modulates Mouse Hippocampal Progenitor Cell Lineages
... most well known role, Notch1 maintains NPCs in the undifferentiated state by inhibiting neuronal differentiation. It was reported that Notch1 inhibits neurogenesis by turning CSL-SKIP into an activator and stimulating the transcription of Hes1 and Hes5 [17]. Moreover, transient activation of Notch1 ...
... most well known role, Notch1 maintains NPCs in the undifferentiated state by inhibiting neuronal differentiation. It was reported that Notch1 inhibits neurogenesis by turning CSL-SKIP into an activator and stimulating the transcription of Hes1 and Hes5 [17]. Moreover, transient activation of Notch1 ...
Comparing The Effects of Small Molecules BIX
... treated with different combinations of small molecules for 7 or 14 days in concomitant with Oct4 exogenous expression were compared for expression of pluripotency markers. Total RNA was isolated from the rims of the injected ventricle and quantitative polymerase chain reaction (PCR) was performed to ...
... treated with different combinations of small molecules for 7 or 14 days in concomitant with Oct4 exogenous expression were compared for expression of pluripotency markers. Total RNA was isolated from the rims of the injected ventricle and quantitative polymerase chain reaction (PCR) was performed to ...
Microtubules Regulate Dynamic Organization of Vacuoles in
... actin microfilaments, rather than microtubules, and that their maintenance and distribution are actin dependent (Ovecka et al. 2005, Higaki et al. 2006). To investigate vacuolar morphology and its regulatory mechanisms, we have, in this study, established a new visualization system using the moss, Ph ...
... actin microfilaments, rather than microtubules, and that their maintenance and distribution are actin dependent (Ovecka et al. 2005, Higaki et al. 2006). To investigate vacuolar morphology and its regulatory mechanisms, we have, in this study, established a new visualization system using the moss, Ph ...
459 Resources
... • In mitochondria, the process of cellular respiration combines food molecules with oxygen. This series of chemical reactions releases energy for the cell's activities. • The energy in light is captured and stored in food molecules during the process of photosynthesis. Plants, algae, and some bacter ...
... • In mitochondria, the process of cellular respiration combines food molecules with oxygen. This series of chemical reactions releases energy for the cell's activities. • The energy in light is captured and stored in food molecules during the process of photosynthesis. Plants, algae, and some bacter ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.