![Pleiotropy of leptin receptor signalling is defined by distinct roles of](http://s1.studyres.com/store/data/017472292_1-226bf87327737e3ec112542bfc272d87-300x300.png)
Pleiotropy of leptin receptor signalling is defined by distinct roles of
... As a class I cytokine receptor, LEPRb activates the janus kinase ⁄ signal transducer and activator of transcription (JAK ⁄ STAT) signalling pathway [10,11]. Ligand binding to LEPRb results in the activation of JAK2 by transphosphorylation and subsequent phosphorylation of tyrosine residues in the cy ...
... As a class I cytokine receptor, LEPRb activates the janus kinase ⁄ signal transducer and activator of transcription (JAK ⁄ STAT) signalling pathway [10,11]. Ligand binding to LEPRb results in the activation of JAK2 by transphosphorylation and subsequent phosphorylation of tyrosine residues in the cy ...
Mixed Lineage Kinase Domain-like Protein Mediates Necrosis
... The importance of necrotic cell death has been illustrated by several recent findings. Breeding onto a RIP3 knockout background rescues the developmental defects of caspase-8 knockout mice. This indicates that the cardiac, vascular, and hematopoietic defects that occur during the development of casp ...
... The importance of necrotic cell death has been illustrated by several recent findings. Breeding onto a RIP3 knockout background rescues the developmental defects of caspase-8 knockout mice. This indicates that the cardiac, vascular, and hematopoietic defects that occur during the development of casp ...
Interplay between SCARECROW, GA and LIKE
... Regulated cell division is critical for the development of multi-cellular organisms. In the Arabidopsis root, SCARECROW (SCR) is required for the first cell division, but represses the subsequent, longitudinal asymmetric cell divisions that generate the two cell types of the ground tissue – cortex a ...
... Regulated cell division is critical for the development of multi-cellular organisms. In the Arabidopsis root, SCARECROW (SCR) is required for the first cell division, but represses the subsequent, longitudinal asymmetric cell divisions that generate the two cell types of the ground tissue – cortex a ...
Localised axial progenitor cell populations in the avian tail bud are
... anteroposterior identity of axial cells (reviewed by Deschamps and van Nes, 2005), and can control the timing of ingression of mesodermal precursors from the epiblast (Iimura and Pourquié, 2006). It is therefore of interest to establish whether Hox gene expression is determined in axial progenitor c ...
... anteroposterior identity of axial cells (reviewed by Deschamps and van Nes, 2005), and can control the timing of ingression of mesodermal precursors from the epiblast (Iimura and Pourquié, 2006). It is therefore of interest to establish whether Hox gene expression is determined in axial progenitor c ...
Cell cycle behavior of human HP1 subtypes: distinct
... formaldehyde and 0.2% glutaraldehyde in DMEM culture medium for 20-30 minutes at room temperature. Fixed cells were then treated twice with 0.1% sodium borohydrate in phosphate-buffered saline (PBS) for 15 minutes at room temperature. The cells were then permeabilized with 0.1% Triton X-100 in PBS f ...
... formaldehyde and 0.2% glutaraldehyde in DMEM culture medium for 20-30 minutes at room temperature. Fixed cells were then treated twice with 0.1% sodium borohydrate in phosphate-buffered saline (PBS) for 15 minutes at room temperature. The cells were then permeabilized with 0.1% Triton X-100 in PBS f ...
phosphatases and differentiation of the golgi apparatus
... samples were used to define tissue types and determine the extent of penetration of the reaction mixture. In initial experiments run to determine hydrolysis time, samples for study by light microscopy were treated with ammonium sulphide. Phase-contrast microscopic observation of thick, plastic-embed ...
... samples were used to define tissue types and determine the extent of penetration of the reaction mixture. In initial experiments run to determine hydrolysis time, samples for study by light microscopy were treated with ammonium sulphide. Phase-contrast microscopic observation of thick, plastic-embed ...
the Golgi Apparatus as the central station of the
... (c) the extracellular medium. We distinguish two types of secretion: 1.1. Constitutive secretion. As soon as lipids and proteins are synthesized, they are continuously transported and secreted to their final destination. This secretion happens in all cells. An example for a protein that follows is c ...
... (c) the extracellular medium. We distinguish two types of secretion: 1.1. Constitutive secretion. As soon as lipids and proteins are synthesized, they are continuously transported and secreted to their final destination. This secretion happens in all cells. An example for a protein that follows is c ...
Two distinct sources for a population of maturing axial
... known to be expressed in the primitive streak and tail bud. Consistent with the expression patterns previously reported for these genes (Takada et al., 1994; Crossley and Martin, 1995; Herrmann, 1991; Kispert and Herrmann, 1994; Dush and Martin, 1992; Ang et al., 1993), we observed Wnt3a, Fgf8, T ( ...
... known to be expressed in the primitive streak and tail bud. Consistent with the expression patterns previously reported for these genes (Takada et al., 1994; Crossley and Martin, 1995; Herrmann, 1991; Kispert and Herrmann, 1994; Dush and Martin, 1992; Ang et al., 1993), we observed Wnt3a, Fgf8, T ( ...
Sampathkumar-2011-Live Cell Imaging Re - Max
... In eukaryotic cells, the actin and microtubule (MT) cytoskeletal networks are dynamic structures that organize intracellular processes and facilitate their rapid reorganization. In plant cells, actin filaments (AFs) and MTs are essential for cell growth and morphogenesis. However, dynamic interactio ...
... In eukaryotic cells, the actin and microtubule (MT) cytoskeletal networks are dynamic structures that organize intracellular processes and facilitate their rapid reorganization. In plant cells, actin filaments (AFs) and MTs are essential for cell growth and morphogenesis. However, dynamic interactio ...
The structure of secondary cell wall polymers: how
... any impact on the biosynthesis of the SCWPs remains to be established. Interactions of SCWPs and S-layers from Bacillaceae ...
... any impact on the biosynthesis of the SCWPs remains to be established. Interactions of SCWPs and S-layers from Bacillaceae ...
PDF
... laboratory and others have reported that Cbl-b−/− mice are resistant to the outgrowth of spontaneous and transplantable tumors (9–11). In addition to T cell-mediated effects, it has recently been reported that Cbl-b−/− mice have enhanced NK cell-mediated tumor immunity (14). As a result of these stu ...
... laboratory and others have reported that Cbl-b−/− mice are resistant to the outgrowth of spontaneous and transplantable tumors (9–11). In addition to T cell-mediated effects, it has recently been reported that Cbl-b−/− mice have enhanced NK cell-mediated tumor immunity (14). As a result of these stu ...
Involvement of retinoic acid-inducible gene- BEAS-2B cells
... expression of IP-10 mRNA was enhanced. Expression of MCP1 mRNA was not affected by overexpression of RIG-I. Upregulation of STAT1 in the cells transfected with RIG-I (full) cDNA was confirmed (fig. 4b). Transfection of the cells with cDNA encoding deletion mutants had no effect on expression of STAT ...
... expression of IP-10 mRNA was enhanced. Expression of MCP1 mRNA was not affected by overexpression of RIG-I. Upregulation of STAT1 in the cells transfected with RIG-I (full) cDNA was confirmed (fig. 4b). Transfection of the cells with cDNA encoding deletion mutants had no effect on expression of STAT ...
Grape Berry Vacuole - American Journal of Enology and Viticulture
... on tonoplast sugar transporters is still limited. However, some tonoplast monosaccharide transporters (TMT) have been recently reported as mediating a proton-coupled antiport mechanism. Three AtTMT (Arabidopsis thaliana tonoplast monosaccharide transporters) isoforms were localized at the tonoplast ...
... on tonoplast sugar transporters is still limited. However, some tonoplast monosaccharide transporters (TMT) have been recently reported as mediating a proton-coupled antiport mechanism. Three AtTMT (Arabidopsis thaliana tonoplast monosaccharide transporters) isoforms were localized at the tonoplast ...
IL-5 Receptor-mediated Tyrosine Phosphorylation of SH2
... The shc gene codes for three protein products of "~46, 52, and 66 kD (21) containing a single COOH-terminal SH2 domain. The tyrosine-phosphorylated Shc binds to Grb2 which activates Sos protein, a Ras nucleotide-exchange protein (22). Ras has been shown to work downstream of tyrosine kinases in the ...
... The shc gene codes for three protein products of "~46, 52, and 66 kD (21) containing a single COOH-terminal SH2 domain. The tyrosine-phosphorylated Shc binds to Grb2 which activates Sos protein, a Ras nucleotide-exchange protein (22). Ras has been shown to work downstream of tyrosine kinases in the ...
BIOLOGY I - one credit - COMPETENCIES AND OBJECTIVES
... 1. Apply inquiry-based and problem-solving processes and skills to scientific investigations. a. Conduct a scientific investigation demonstrating safe procedures and proper care of laboratory equipment. (DOK 2) Safety rules and symbols Proper use and care of the compound light microscope, slides ...
... 1. Apply inquiry-based and problem-solving processes and skills to scientific investigations. a. Conduct a scientific investigation demonstrating safe procedures and proper care of laboratory equipment. (DOK 2) Safety rules and symbols Proper use and care of the compound light microscope, slides ...
Denitrification of water in a microbial fuel cell (MFC) using seawater
... The sea contains various microbes which have an ability to reduce and oxidize substances like iron, sulphur, and nitrate. Most of these processes happen in the seawater, but can also be applied for purification of wastewater. In the present work, a consortium of seawater bacteria has been used for t ...
... The sea contains various microbes which have an ability to reduce and oxidize substances like iron, sulphur, and nitrate. Most of these processes happen in the seawater, but can also be applied for purification of wastewater. In the present work, a consortium of seawater bacteria has been used for t ...
Biology I Framework Competencies
... Importance of ions in biological processes b. Develop a logical argument defending water as an essential component of living systems (e.g., unique bonding and properties including polarity, high specific heat, surface tension, hydrogen bonding, adhesion, cohesion, and expansion upon freezing). (DOK ...
... Importance of ions in biological processes b. Develop a logical argument defending water as an essential component of living systems (e.g., unique bonding and properties including polarity, high specific heat, surface tension, hydrogen bonding, adhesion, cohesion, and expansion upon freezing). (DOK ...
Integration of the olfactory code across dendritic
... somatic signals typically required the activation of multiple claws. Many odors activated one or two claws, but only in a small proportion of those cases was there an accompanying somatic response. In contrast, when an odor activated several claws, somatic responses were observed much more frequentl ...
... somatic signals typically required the activation of multiple claws. Many odors activated one or two claws, but only in a small proportion of those cases was there an accompanying somatic response. In contrast, when an odor activated several claws, somatic responses were observed much more frequentl ...
Trafficking of the human transferrin receptor in plant cells: effects of
... shown to bind several mammalian network proteins, and it also interacts with At-AP180, a monomeric adaptor homologue from Arabidopsis that functions as a plant clathrin assembly protein (Barth and Holstein, 2004). On the other hand, direct involvement of CCVs in RME has not yet been demonstrated, an ...
... shown to bind several mammalian network proteins, and it also interacts with At-AP180, a monomeric adaptor homologue from Arabidopsis that functions as a plant clathrin assembly protein (Barth and Holstein, 2004). On the other hand, direct involvement of CCVs in RME has not yet been demonstrated, an ...
Overview of Cells and Body Systems - Moodle
... Introduction to Cells, Tissues and Body Systems Life Science FLSS All images used are taken from copyright-free sources e.g. Wikicommons Media or produced by UWS staff and are for illustrative purposes. ...
... Introduction to Cells, Tissues and Body Systems Life Science FLSS All images used are taken from copyright-free sources e.g. Wikicommons Media or produced by UWS staff and are for illustrative purposes. ...
PDF + SI - Development - The Company of Biologists
... expression of the SOMBRERO transcription factor (Fendrych et al., 2014), in a mechanism that could be functionally similar to that mediated by ZOU. More detailed biophysical analysis as well as detailed cell structure analysis will help to clarify whether molecular pathways are shared between these ...
... expression of the SOMBRERO transcription factor (Fendrych et al., 2014), in a mechanism that could be functionally similar to that mediated by ZOU. More detailed biophysical analysis as well as detailed cell structure analysis will help to clarify whether molecular pathways are shared between these ...
Control of the Actin Cytoskeleton in Plant Cell Growth
... monomer incorporation at the barbed end beyond the limits of free diffusion (80). Unlike the putative plant Arp2/3 complex, plant formins have been studied to some extent in vitro. Four plant formins (AtFH1, AtFH4, AtFH5, and AtFH8) have been shown to nucleate purified actin, and allow extension from ...
... monomer incorporation at the barbed end beyond the limits of free diffusion (80). Unlike the putative plant Arp2/3 complex, plant formins have been studied to some extent in vitro. Four plant formins (AtFH1, AtFH4, AtFH5, and AtFH8) have been shown to nucleate purified actin, and allow extension from ...
Diazonamide A and a Synthetic Structural Analog
... cells (DiSaia et al., 1972). The cells were seeded at 2 ⫻ 105 cells per culture in 10 ml of RPMI 1640 medium supplemented with 5% heat-inactivated fetal bovine serum, 2 mM L-glutamine, 200 units/ml of penicillin G, and 200 g/ml of streptomycin. Diazonamide A or an equivalent amount of dimethyl sulf ...
... cells (DiSaia et al., 1972). The cells were seeded at 2 ⫻ 105 cells per culture in 10 ml of RPMI 1640 medium supplemented with 5% heat-inactivated fetal bovine serum, 2 mM L-glutamine, 200 units/ml of penicillin G, and 200 g/ml of streptomycin. Diazonamide A or an equivalent amount of dimethyl sulf ...
Is the Development of Orientation Selectivity Instructed by Activity?
... salamander retina, consistent with the idea, discussed in Miller (1994), that such a pattern of spontaneous activity can arise naturally from the circuitry that induces retinal and/or LGN center-surround receptive field structure). As discussed above, the second experiment was recently carried out i ...
... salamander retina, consistent with the idea, discussed in Miller (1994), that such a pattern of spontaneous activity can arise naturally from the circuitry that induces retinal and/or LGN center-surround receptive field structure). As discussed above, the second experiment was recently carried out i ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.