Cell Membrane and Sub Cellular Components
... These proteins are important for cell because they are responsible for various biological activities. Approximately a third of thegenes in yeast code specifically for them, and this number is even higher in multicellular organisms. The cell membrane, being exposed to the outside environment, is an i ...
... These proteins are important for cell because they are responsible for various biological activities. Approximately a third of thegenes in yeast code specifically for them, and this number is even higher in multicellular organisms. The cell membrane, being exposed to the outside environment, is an i ...
LvDelta induces mesoderm and endoderm
... mMachine kit (Ambion). In addition, mRNA encoding a truncated form of LvDelta was used as a control. Using the Quik Change SiteDirected Mutagenesis kit (Stratagene) a G-to-T point mutation was introduced at nucleotide position 981, which generated an internal stop codon following amino acid 135. Thi ...
... mMachine kit (Ambion). In addition, mRNA encoding a truncated form of LvDelta was used as a control. Using the Quik Change SiteDirected Mutagenesis kit (Stratagene) a G-to-T point mutation was introduced at nucleotide position 981, which generated an internal stop codon following amino acid 135. Thi ...
Embryology 8
... Mitotic figures are found in the cytotrophoblast but not in the syncytiotrophoblast.Thus, cells in the cytotrophoblast divide and migrate into the syncytiotrophoblast, where they fuse and lose their individual cell membranes, the syncytiotrophoblast secrete HCG hormone that used in the pregnancy t ...
... Mitotic figures are found in the cytotrophoblast but not in the syncytiotrophoblast.Thus, cells in the cytotrophoblast divide and migrate into the syncytiotrophoblast, where they fuse and lose their individual cell membranes, the syncytiotrophoblast secrete HCG hormone that used in the pregnancy t ...
A nuclear lamin is required for cytoplasmic organization and egg polarity in Drosophila. Nature Cell Biology 3, 848-851. pdf
... membrane proteins, so they can influence chromosome organization and possibly gene expression6,10,16. gurken transcripts are synthesized in the oocyte nucleus and must interact with the Squid hnRNP protein and possibly K10, a putative RNA-binding protein, in order to localize properly after export i ...
... membrane proteins, so they can influence chromosome organization and possibly gene expression6,10,16. gurken transcripts are synthesized in the oocyte nucleus and must interact with the Squid hnRNP protein and possibly K10, a putative RNA-binding protein, in order to localize properly after export i ...
The argos Gene Encodes a Diffusible Factor
... (data not shown). We find that there are typically one or two extra photoreceptors, implying that there is no additional neural recruitment after the mystery cells are transformed. In a few cases we see as many as 20 cone cells in a single cluster. These may be the products of the ommatidial fusions ...
... (data not shown). We find that there are typically one or two extra photoreceptors, implying that there is no additional neural recruitment after the mystery cells are transformed. In a few cases we see as many as 20 cone cells in a single cluster. These may be the products of the ommatidial fusions ...
Chemotaxis
... By integrating over time it solves both problems Berg (1988) • if memory is 1 min and bacterium is moving at 30 m sec-1, concentration comparison is being made over ~2 mm, or 1000-bodylengths – lessens accuracy needed to 1 in 10 • memory is actually only ~1-10 sec • Why not have longer memory? - inf ...
... By integrating over time it solves both problems Berg (1988) • if memory is 1 min and bacterium is moving at 30 m sec-1, concentration comparison is being made over ~2 mm, or 1000-bodylengths – lessens accuracy needed to 1 in 10 • memory is actually only ~1-10 sec • Why not have longer memory? - inf ...
TOPIC 1. CELL CHARACTERISTICS AND DIGESTIBILITIES What
... alkaloids, essential oils, and organic acids. Those substances, apparently not involved in the basic metabolism of a plant, do reduce herbivory. It must also be pointed out that wild ruminants make up a very small portion of the worldl's herbivores; insects, though much smaller, have the potential f ...
... alkaloids, essential oils, and organic acids. Those substances, apparently not involved in the basic metabolism of a plant, do reduce herbivory. It must also be pointed out that wild ruminants make up a very small portion of the worldl's herbivores; insects, though much smaller, have the potential f ...
Microcavity Lasers for Cancer Cell Detection
... vertical cavity surface-emitting lasers for micro-optical-mechanical systems,” Applied Physics Letters, vol 69 (11), 9 ...
... vertical cavity surface-emitting lasers for micro-optical-mechanical systems,” Applied Physics Letters, vol 69 (11), 9 ...
Cells without the calnexin/calreticulin central region are viable
... have isolated cnx1+, the S. pombe calnexin homologue, and have shown that it encodes a protein essential for viability (Jannatipour and Rokeach, 1995; Parlati et al., 1995). Like its mammalian counterparts, Cnx1p is a type I ER-membrane protein containing the characteristic highly conserved central ...
... have isolated cnx1+, the S. pombe calnexin homologue, and have shown that it encodes a protein essential for viability (Jannatipour and Rokeach, 1995; Parlati et al., 1995). Like its mammalian counterparts, Cnx1p is a type I ER-membrane protein containing the characteristic highly conserved central ...
Root cytoskeleton: its role in perception of and response to gravity
... gravity. For instance, the plant cytoskeleton is involved in intracellular signalling (Tan and Boss 1992; Xu et al. 1992; Drobak 1993; Lisanti et al. 1994), cellular motilities (Kamiya 1981; Williamson 1993), cell growth (Thimann et al. 1992; BalusÏ ka et al. 1997a; Reichelt et al. 1997), and establ ...
... gravity. For instance, the plant cytoskeleton is involved in intracellular signalling (Tan and Boss 1992; Xu et al. 1992; Drobak 1993; Lisanti et al. 1994), cellular motilities (Kamiya 1981; Williamson 1993), cell growth (Thimann et al. 1992; BalusÏ ka et al. 1997a; Reichelt et al. 1997), and establ ...
Multicellular Organisms National 5 Biology: Learning Outcomes
... 4. Explain how xylem vessels are specialised to their function. 5. Describe the movement of water through a plant from the root to the leaf. 6. Define transpiration. 7. Give examples of environmental factors that could increase transpiration ...
... 4. Explain how xylem vessels are specialised to their function. 5. Describe the movement of water through a plant from the root to the leaf. 6. Define transpiration. 7. Give examples of environmental factors that could increase transpiration ...
PIERGIORGIO PETTAZZONI
... diseases. It has been demonstrated that HNE plays an important role in the control of tumour cell proliferation and differentiation. One micromolar HNE, a dose similar to those detected in normal cells, inhibits proliferation and induces differentiation of HL-60 human leukemic cells. This HNE concen ...
... diseases. It has been demonstrated that HNE plays an important role in the control of tumour cell proliferation and differentiation. One micromolar HNE, a dose similar to those detected in normal cells, inhibits proliferation and induces differentiation of HL-60 human leukemic cells. This HNE concen ...
Andrei Fagarasanu Department of Cell Biology Supervisor: Dr
... In the current study, published in the Journal of Cell Biology, I constructed Myo2p mutants specifically defective in transporting peroxisomes but which preserved the ability to carry other organelles. I then analyzed the dynamics of Inp2p in cells harboring such Myo2p mutants. I found that Inp2p co ...
... In the current study, published in the Journal of Cell Biology, I constructed Myo2p mutants specifically defective in transporting peroxisomes but which preserved the ability to carry other organelles. I then analyzed the dynamics of Inp2p in cells harboring such Myo2p mutants. I found that Inp2p co ...
Characterization of Organelles in the Vacuolar
... Each 1 g of the packed cells was homogenized in 2 ml of the following buffers. The buffer of 10 mM Tris-HCl, pH 7.5, including proteinase inhibitors (Complete Mini, Boehringer Mannheim, Tokyo, Japan) was used for SP-GFP/BY2, SP-GFP-HDEL/BY2 and SP-GFP-2SC/ BY2 that accumulated soluble GFPs. The othe ...
... Each 1 g of the packed cells was homogenized in 2 ml of the following buffers. The buffer of 10 mM Tris-HCl, pH 7.5, including proteinase inhibitors (Complete Mini, Boehringer Mannheim, Tokyo, Japan) was used for SP-GFP/BY2, SP-GFP-HDEL/BY2 and SP-GFP-2SC/ BY2 that accumulated soluble GFPs. The othe ...
Characterization of the unconventional myosin VIII in plant cells and
... The distribution of myosin VIII, actin and microtubules during stages in the cell cycle in root meristems in higher plants are shown in Figures 2 and 3 and are summarised in the schematic drawings in Figure 6. At the onset of mitosis (prophase), actin is localised as dense irregular networks surroun ...
... The distribution of myosin VIII, actin and microtubules during stages in the cell cycle in root meristems in higher plants are shown in Figures 2 and 3 and are summarised in the schematic drawings in Figure 6. At the onset of mitosis (prophase), actin is localised as dense irregular networks surroun ...
supplementary information
... 0.01) is γS,high = 0.46 ± 0.01 hr-1 (s.e.m.). Consistent results were also obtained by direct observation of growth at low density by periodic cell plating (data not shown). Dilution experiments in glucose culture yield similar growth rates at high and low density (circles and panel a). All growth r ...
... 0.01) is γS,high = 0.46 ± 0.01 hr-1 (s.e.m.). Consistent results were also obtained by direct observation of growth at low density by periodic cell plating (data not shown). Dilution experiments in glucose culture yield similar growth rates at high and low density (circles and panel a). All growth r ...
PPT - Altogen Biosystems
... Transfection Reagent (Prostate Carcinoma Cells) Altogen Biosystems offers the DU145 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The DU145 Transfection Reagent is an advanced lipid formulation reagent, and it has been developed to provide high transfection ...
... Transfection Reagent (Prostate Carcinoma Cells) Altogen Biosystems offers the DU145 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The DU145 Transfection Reagent is an advanced lipid formulation reagent, and it has been developed to provide high transfection ...
1 a dictyostelium mutant with reduced lysozyme levels compensates
... trafficking step in the cell (2) and the LYST proteins, which when mutated cause enlarged lysosomes manifested as Chediak-Higashi Syndrome (3). The second class of mutations affects lysosomal enzymes with metabolic roles. These enzymes degrade macromolecules that the cell wants to dispose of, produc ...
... trafficking step in the cell (2) and the LYST proteins, which when mutated cause enlarged lysosomes manifested as Chediak-Higashi Syndrome (3). The second class of mutations affects lysosomal enzymes with metabolic roles. These enzymes degrade macromolecules that the cell wants to dispose of, produc ...
Zinc Transporter of Arabidopsis thaliana AtMTP1 is Localized
... 1, 200 and 500 µM ZnSO4 for 3 d (Fig. 7B). The mtp1-1 mutant plantlets grew normally in 0 and 1 µM Zn; however, they showed severe chlorosis of leaves and suppression of leaf development in 200 and 500 µM Zn (Fig. 7B). It should be noted that the medium may contain a trace amount of Zn even in the 0 ...
... 1, 200 and 500 µM ZnSO4 for 3 d (Fig. 7B). The mtp1-1 mutant plantlets grew normally in 0 and 1 µM Zn; however, they showed severe chlorosis of leaves and suppression of leaf development in 200 and 500 µM Zn (Fig. 7B). It should be noted that the medium may contain a trace amount of Zn even in the 0 ...
Learning about the Importance of Mutation
... phase of the cell cycle [46-48]. For survival, the premalignant, probably also early malignant, cells need to find a way, such as having some additional genetic or epigenetic change, to desensitize such inhibition. Once having progressed to an advanced stage and having become dominant, cancer cells ...
... phase of the cell cycle [46-48]. For survival, the premalignant, probably also early malignant, cells need to find a way, such as having some additional genetic or epigenetic change, to desensitize such inhibition. Once having progressed to an advanced stage and having become dominant, cancer cells ...
Molecular identification of 26 syntaxin genes and
... Characteristics of Paramecium syntaxins On the basis of the Paramecium sequencing project of the macronucleus (27), we were able to identify and annotate 26 syntaxin coding sequences by manual assembly of single reads during the early steps of the genome project, which were deposited at European Mol ...
... Characteristics of Paramecium syntaxins On the basis of the Paramecium sequencing project of the macronucleus (27), we were able to identify and annotate 26 syntaxin coding sequences by manual assembly of single reads during the early steps of the genome project, which were deposited at European Mol ...
Chemokine-Induced Migration Inhibits Integrin
... time to time creates long “dendrites” (Fig. 2B and Ref. (17). Strikingly, when A39R was added at the beginning of the culture together with LPS, it completely prevented the formation of membrane processes by DCs (Fig. 2C) as well as their adhesion to plastic. This effect was dependent on plexin C1 a ...
... time to time creates long “dendrites” (Fig. 2B and Ref. (17). Strikingly, when A39R was added at the beginning of the culture together with LPS, it completely prevented the formation of membrane processes by DCs (Fig. 2C) as well as their adhesion to plastic. This effect was dependent on plexin C1 a ...
DCE
... Directory Services • DCE Cell Directory Service (CDS) is the mechanism for logically naming objects within a DCE cell (a group of client and server machines) • Applications identify resources by name, without needing to know where the resources are located • DCE cells can also participate in a wo ...
... Directory Services • DCE Cell Directory Service (CDS) is the mechanism for logically naming objects within a DCE cell (a group of client and server machines) • Applications identify resources by name, without needing to know where the resources are located • DCE cells can also participate in a wo ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.