Myc Requires Distinct E2F Activities to Induce S Phase
... mouse embryo fibroblasts were transfected with 1 g of CMV-GFP and either 5 g of control vector (⫺), 5 g of CMV-Myc (Myc), or 2 g of CMV-E2F1 and 2 g of CMV-Dp1 (E2F1) as described in Experimental Procedures. Cell survival was measured as described in Experimental Procedures. At right, wild-type ...
... mouse embryo fibroblasts were transfected with 1 g of CMV-GFP and either 5 g of control vector (⫺), 5 g of CMV-Myc (Myc), or 2 g of CMV-E2F1 and 2 g of CMV-Dp1 (E2F1) as described in Experimental Procedures. Cell survival was measured as described in Experimental Procedures. At right, wild-type ...
Carr_Flagellum.pps
... A flagellum is on the end of every sperm cell Without a flagellum sperm would not be able to fertilize an egg ...
... A flagellum is on the end of every sperm cell Without a flagellum sperm would not be able to fertilize an egg ...
Genome-Wide Identification of Target Genes for the Key B
... quiescent state, since lack of Ets1 leads to premature differentiation into ASCs (22, 23). This is accompanied by a loss of B cell tolerance to self-antigens (24). Ets1−/− mice also lack marginal zone type B cells, possibly because of depletion due to excessive differentiation to ASCs (23, 25). In k ...
... quiescent state, since lack of Ets1 leads to premature differentiation into ASCs (22, 23). This is accompanied by a loss of B cell tolerance to self-antigens (24). Ets1−/− mice also lack marginal zone type B cells, possibly because of depletion due to excessive differentiation to ASCs (23, 25). In k ...
Plant Molecular Biology
... these protoplasts, harvested after the indicated period of DEX treatment, using genomic DNA from Bax transgenic plants as probe. ...
... these protoplasts, harvested after the indicated period of DEX treatment, using genomic DNA from Bax transgenic plants as probe. ...
Should reflectance confocal microscopy be the gold standard for
... “Reflectance confocal microscopy is sufficiently developed to enable real-time diagnosis of basal cell carcinoma with an accuracy and sensitivity almost comparable to histological analysis.” ...
... “Reflectance confocal microscopy is sufficiently developed to enable real-time diagnosis of basal cell carcinoma with an accuracy and sensitivity almost comparable to histological analysis.” ...
Haematopoietic and immune defects associated with GATA2 mutation
... zinc finger domains. Mutations appear to cause haplo-insufficiency, which is known to impair haematopoietic stem cell survival in animal models. Management includes genetic counselling, prevention of infection, cancer surveillance, haematopoietic monitoring and, ultimately, stem cell transplantation ...
... zinc finger domains. Mutations appear to cause haplo-insufficiency, which is known to impair haematopoietic stem cell survival in animal models. Management includes genetic counselling, prevention of infection, cancer surveillance, haematopoietic monitoring and, ultimately, stem cell transplantation ...
Octamer-binding transcription factors: genomics and functions
... and sequence of the hypervariable POU linker also influences the DNA-binding specificity (40, 41). In addition, the bases flanking the core recognition sequence make a modest but significant contribution to the DNAbinding affinity of the POU domain (21). These influences should contribute to the sel ...
... and sequence of the hypervariable POU linker also influences the DNA-binding specificity (40, 41). In addition, the bases flanking the core recognition sequence make a modest but significant contribution to the DNAbinding affinity of the POU domain (21). These influences should contribute to the sel ...
reviews
... The primary tissue types of the mouse| Molecular embryo —Cell Biology trophectoderm, epiblast and primitive endoderm — are established before implantation at around embryonic day 4.5 (E4.5). a | At E2.5, the eight blastomeres initially show overlapping expression of two transcription factors, caudal ...
... The primary tissue types of the mouse| Molecular embryo —Cell Biology trophectoderm, epiblast and primitive endoderm — are established before implantation at around embryonic day 4.5 (E4.5). a | At E2.5, the eight blastomeres initially show overlapping expression of two transcription factors, caudal ...
10. Early Amphibian Development
... 1) Anteriorposterior, dorsalventral, and leftright axes are specified by events triggered at fertilization and realized during gastrulation. 2) Mesoderm is determined by transcription factors and paracrine factors from the vegetal region. 3) Of all tissues in the Xenopus pregastrula, only th ...
... 1) Anteriorposterior, dorsalventral, and leftright axes are specified by events triggered at fertilization and realized during gastrulation. 2) Mesoderm is determined by transcription factors and paracrine factors from the vegetal region. 3) Of all tissues in the Xenopus pregastrula, only th ...
Analysis of Cell Divisions Patterns in the Arabidopsis Shoot Apical
... elongation is often confused with the direction of growth. Cells are asymmetrical and hence a principal direction of cell elongation can easily be calculated (e.g., the principal axis of inertia or principal component of a segmentation). The assumption is often made that because the cell is more elo ...
... elongation is often confused with the direction of growth. Cells are asymmetrical and hence a principal direction of cell elongation can easily be calculated (e.g., the principal axis of inertia or principal component of a segmentation). The assumption is often made that because the cell is more elo ...
Eur J - HAL
... 44 amino acid residues are ultimately removed from the amino terminus, yielding an active intermediate 48 kDa single-chain molecule. This main proteolytic activation event most likely depends on the action of cysteine lysosomal and/or aspartic proteases [10-12]. Depending on the cell type, cath-D ma ...
... 44 amino acid residues are ultimately removed from the amino terminus, yielding an active intermediate 48 kDa single-chain molecule. This main proteolytic activation event most likely depends on the action of cysteine lysosomal and/or aspartic proteases [10-12]. Depending on the cell type, cath-D ma ...
Mechanisms of vertebrate segmentation
... cells emerging from this part of the streak also contribute to the notochord (Spratt, 1955; Rosenquist, 1966; Nicolet, 1971; Tarn & Beddington, 1987). As development proceeds, neurulation takes place in the midline, and the segmental plates come to flank the neural tube and notochord (Figs 1A, 2). I ...
... cells emerging from this part of the streak also contribute to the notochord (Spratt, 1955; Rosenquist, 1966; Nicolet, 1971; Tarn & Beddington, 1987). As development proceeds, neurulation takes place in the midline, and the segmental plates come to flank the neural tube and notochord (Figs 1A, 2). I ...
Involvement of the Vacuoles of the Endodermis in
... parental zig-1 plants showed little response even after 24 hr. In addition, their lateral stems, which in the parental zig-1 mutant curl downward, grew upward (Figure 2B). Interestingly, however, pSCR::ZIG could not complement the morphological phenotype of zig-1 completely, because the transgenic m ...
... parental zig-1 plants showed little response even after 24 hr. In addition, their lateral stems, which in the parental zig-1 mutant curl downward, grew upward (Figure 2B). Interestingly, however, pSCR::ZIG could not complement the morphological phenotype of zig-1 completely, because the transgenic m ...
EVEN/ODD
... b. other cells are trapped making a clot that closes the torn vessel c. scabs are dried clots 4. plasma – light yellow liquid e. Blood Types i. These are determined by the presence or absence of certain antigens – substances that can trigger an immune response if they are foreign to the body. Since ...
... b. other cells are trapped making a clot that closes the torn vessel c. scabs are dried clots 4. plasma – light yellow liquid e. Blood Types i. These are determined by the presence or absence of certain antigens – substances that can trigger an immune response if they are foreign to the body. Since ...
Chapter 7: Cellular Structure and Function - Bellbrook
... transmission electron microscope (TEM) because electrons are passed, or transmitted, through a specimen to a fluorescent screen. Thick parts of the specimen absorb more electrons than thin parts, forming a black-andwhite shaded image of the specimen. Transmission electron microscopes can magnify up ...
... transmission electron microscope (TEM) because electrons are passed, or transmitted, through a specimen to a fluorescent screen. Thick parts of the specimen absorb more electrons than thin parts, forming a black-andwhite shaded image of the specimen. Transmission electron microscopes can magnify up ...
Forces That Move Ions and Molecules
... needed by living cells in the face of these passive movements. Much of a cell's supply of metabolic energy may be spent maintaining these processes. Most of a red blood cell's metabolic energy is used to maintain the imbalance between exterior and interior sodium and potassium levels required by the ...
... needed by living cells in the face of these passive movements. Much of a cell's supply of metabolic energy may be spent maintaining these processes. Most of a red blood cell's metabolic energy is used to maintain the imbalance between exterior and interior sodium and potassium levels required by the ...
Review Convergent Extension - Massachusetts Institute of Technology
... the axial tissue do they undergo mediolateral intercalation (Thorogood and Wood, 1987; Trinkaus et al., 1992; Wood and Thorogood, 1994). It is important to note that while we present a synthesis of cell behaviors and movements in this review based upon observations made in these three fish, differen ...
... the axial tissue do they undergo mediolateral intercalation (Thorogood and Wood, 1987; Trinkaus et al., 1992; Wood and Thorogood, 1994). It is important to note that while we present a synthesis of cell behaviors and movements in this review based upon observations made in these three fish, differen ...
BIOGRAPHICAL SKETCH NAME: Saghizadeh, Mehrnoosh eRA
... healing and LESC marker patterns in diabetic cornea. We will target diabetic corneal epithelial cells by single microRNA or their combinations to improve function of corneal epithelium. This research could lead to the generation of a new cell source for restoring vision in patients with altered LESC ...
... healing and LESC marker patterns in diabetic cornea. We will target diabetic corneal epithelial cells by single microRNA or their combinations to improve function of corneal epithelium. This research could lead to the generation of a new cell source for restoring vision in patients with altered LESC ...
- Horizon Discovery
... rates of targeted incorporation of the rAAV donor. We sought to test the improvement in targeting frequency in mammalian cells by inhibition of the DNA mismatch repair pathway by RNA interference and the incorporation of negative selection into rAAV donors. We tested if any improvements seen could b ...
... rates of targeted incorporation of the rAAV donor. We sought to test the improvement in targeting frequency in mammalian cells by inhibition of the DNA mismatch repair pathway by RNA interference and the incorporation of negative selection into rAAV donors. We tested if any improvements seen could b ...
Cardiac Cell Lineages that Form the Heart
... Retrospective clonal analysis in the mouse embryo (see Buckingham and Meilhac 2011) indicated that two major lineages contribute to the myocardium of the heart. The first lineage contributes left ventricular myocardium, whereas the second lineage is the source of outflow tract and most right ventric ...
... Retrospective clonal analysis in the mouse embryo (see Buckingham and Meilhac 2011) indicated that two major lineages contribute to the myocardium of the heart. The first lineage contributes left ventricular myocardium, whereas the second lineage is the source of outflow tract and most right ventric ...
An in vitro System to Study Interactions between Bacteria and
... still 70% viable after storage for 4 h. Prolonged storage at 0-4 "C rendered the cells more sensitive to agitation, so for most adhesion experiments the cells were used within 1 h of isolation. For long-term storage, glycerol was added to the cell suspension to a final concentration of 15% (v/v) and ...
... still 70% viable after storage for 4 h. Prolonged storage at 0-4 "C rendered the cells more sensitive to agitation, so for most adhesion experiments the cells were used within 1 h of isolation. For long-term storage, glycerol was added to the cell suspension to a final concentration of 15% (v/v) and ...
PDF
... on the basis of a conserved 86 amino acid coding sequence and exon structure. This gene, htr-wnt-A, is not an obvious homolog of any one of the previously described wnt class proteins. The embryonic expression of htr-w&A has been characterized at the cellular level, using nonradioactive in situ hybr ...
... on the basis of a conserved 86 amino acid coding sequence and exon structure. This gene, htr-wnt-A, is not an obvious homolog of any one of the previously described wnt class proteins. The embryonic expression of htr-w&A has been characterized at the cellular level, using nonradioactive in situ hybr ...
The F8H Glycosyltransferase is a Functional Paralog of FRA8
... that catalyzes the transfer of glucuronic acid onto rhamnogalacturonan II (Iwai et al. 2002). At5g22940 is the closest homolog of FRA8, and its deduced amino acid sequence shares 69% similarity and 59% identity with FRA8 (Fig. 1A); therefore, we designated it as F8H. To examine the expression patter ...
... that catalyzes the transfer of glucuronic acid onto rhamnogalacturonan II (Iwai et al. 2002). At5g22940 is the closest homolog of FRA8, and its deduced amino acid sequence shares 69% similarity and 59% identity with FRA8 (Fig. 1A); therefore, we designated it as F8H. To examine the expression patter ...
Lymphoma Vaccines for Active Immunotherapy of T Cell Are More
... The ability to target multiple tumor Ags may increase the magnitude and diversity of anti-tumor responses, thus preventing tumor from escaping responses limited in repertoire (30). Loading DC with proteins from tumor cell lysates may result in the presentation of a broader array of tumor-specific Ag ...
... The ability to target multiple tumor Ags may increase the magnitude and diversity of anti-tumor responses, thus preventing tumor from escaping responses limited in repertoire (30). Loading DC with proteins from tumor cell lysates may result in the presentation of a broader array of tumor-specific Ag ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.