Dynamin 2 mediates fluid-phase micropinocytosis in epithelial cells
... material Fig. S2B⬘), treatment with either the pooled mixture of Dyn2-siRNA duplexes or Dyn2-siRNA duplex #2 reduced Dyn2 protein levels as well as transferrin uptake by ~70-80% (supplementary material Fig. S2A,C,D). In support of the microinjection experiments shown in Fig. 1, cells treated with Dy ...
... material Fig. S2B⬘), treatment with either the pooled mixture of Dyn2-siRNA duplexes or Dyn2-siRNA duplex #2 reduced Dyn2 protein levels as well as transferrin uptake by ~70-80% (supplementary material Fig. S2A,C,D). In support of the microinjection experiments shown in Fig. 1, cells treated with Dy ...
Course Outline
... These are structures surrounding the outside of the cell envelope. When more defined, they are referred to as a capsule when less defined as a slime layer or glycocalyx. They usually consist of polysaccharide; however, in certain bacilli they are composed of a polypeptide (polyglutamic acid). They a ...
... These are structures surrounding the outside of the cell envelope. When more defined, they are referred to as a capsule when less defined as a slime layer or glycocalyx. They usually consist of polysaccharide; however, in certain bacilli they are composed of a polypeptide (polyglutamic acid). They a ...
mrsa
... was finally isolated and synthesized in large quantities in 1943. Penicillin works by interfering with the bacterial cell wall synthesis. Without a cell wall, the bacterial cells cannot maintain their shape in changing osmotic conditions. This puts significant selective pressure on the microbes to e ...
... was finally isolated and synthesized in large quantities in 1943. Penicillin works by interfering with the bacterial cell wall synthesis. Without a cell wall, the bacterial cells cannot maintain their shape in changing osmotic conditions. This puts significant selective pressure on the microbes to e ...
Final presentation
... Jagera People, the Traditional Owners of the land on which this event is taking place and Elders both past and present. ...
... Jagera People, the Traditional Owners of the land on which this event is taking place and Elders both past and present. ...
Lecture 1 Part I Nordström 13.9.
... • Some make fimbria-like structures called hami • Function to attach archaea to surfaces © 2015 Pearson Education, Ltd. ...
... • Some make fimbria-like structures called hami • Function to attach archaea to surfaces © 2015 Pearson Education, Ltd. ...
Differentiation of Cardiac Myocytes after Mitogen Withdrawal
... By days 2 and 3, the contractile myocytes developed a characteristic morphology consisting of a coarse granular cytoplasm with dense inclusions and a dense round nucleus. By the criteria of morphology and spontaneous contraction, the ventricular myocytes were readily distinguished from nonmuscle cel ...
... By days 2 and 3, the contractile myocytes developed a characteristic morphology consisting of a coarse granular cytoplasm with dense inclusions and a dense round nucleus. By the criteria of morphology and spontaneous contraction, the ventricular myocytes were readily distinguished from nonmuscle cel ...
ANIMAL TISSUES
... matrix with relatively few cells. Connective tissue cells are able to reproduce but not as rapidly as epithelial cells. Most connective tissues have a good blood supply and nerve supply but some do not have them, e.g. cartilage. The matrix of the connective tissue is usually secreted by the connect ...
... matrix with relatively few cells. Connective tissue cells are able to reproduce but not as rapidly as epithelial cells. Most connective tissues have a good blood supply and nerve supply but some do not have them, e.g. cartilage. The matrix of the connective tissue is usually secreted by the connect ...
Shield formation at the onset of zebrafish gastrulation
... Cell transplantations Cells from donors fluorescently labeled with Rhodamine- or Fluorescein-dextran (Molecular Probes) and expressing membranebound (GAP43-) YFP mRNA were transplanted into unlabeled hosts, as described (Reim and Brand, 2002), and monitored using a ...
... Cell transplantations Cells from donors fluorescently labeled with Rhodamine- or Fluorescein-dextran (Molecular Probes) and expressing membranebound (GAP43-) YFP mRNA were transplanted into unlabeled hosts, as described (Reim and Brand, 2002), and monitored using a ...
Shield formation at the onset of zebrafish gastrulation - MPI
... transition. Experiments in which single mesendodermal progenitors were transplanted into places of the gastrula where no other mesendodermal progenitors can be found have shown that these cells ingress in a cell-autonomous manner, suggesting that single-cell ingression is the primary mode for mesend ...
... transition. Experiments in which single mesendodermal progenitors were transplanted into places of the gastrula where no other mesendodermal progenitors can be found have shown that these cells ingress in a cell-autonomous manner, suggesting that single-cell ingression is the primary mode for mesend ...
A Comprehensive Gene Expression Analysis Toward the
... found in mitotic BY-2 cells (McCutcheon et al. 2001), was found in group B. Many kinesin-like proteins that show higher expression at the log phase are unique to plants. Thus, tobacco homologs of some of these, especially those that show the expression pattern classified as group C, might be involve ...
... found in mitotic BY-2 cells (McCutcheon et al. 2001), was found in group B. Many kinesin-like proteins that show higher expression at the log phase are unique to plants. Thus, tobacco homologs of some of these, especially those that show the expression pattern classified as group C, might be involve ...
University of Groningen Bromodeoxyuridine does not contribute to
... rates, we first established baseline SCE rates in both normal and BS cells. We used primary fibroblasts and EBVtransformed lymphoblasts from healthy donors and BS patients for our analysis. Logarithmically growing cells were pulsed with 40 M for one cell division (18 h for fibroblasts, 24 h for lym ...
... rates, we first established baseline SCE rates in both normal and BS cells. We used primary fibroblasts and EBVtransformed lymphoblasts from healthy donors and BS patients for our analysis. Logarithmically growing cells were pulsed with 40 M for one cell division (18 h for fibroblasts, 24 h for lym ...
Meiotic long non-coding meiRNA accumulates as a dot at its genetic
... (longer than 1.0 kb) [10,12]. Mei2 physically interacts with meiRNA and forms a dot structure at the sme2 locus on chromosome II, depending on the transcription of meiRNA [10,11,13]. These observations suggest that the transcribed meiRNA is tethered to its own genetic locus and may play a role as a ...
... (longer than 1.0 kb) [10,12]. Mei2 physically interacts with meiRNA and forms a dot structure at the sme2 locus on chromosome II, depending on the transcription of meiRNA [10,11,13]. These observations suggest that the transcribed meiRNA is tethered to its own genetic locus and may play a role as a ...
Diffusion, Osmosis, and Movement across a Membrane
... Occurs via random kinetic movement (random movement of particles, which is faster at higher temperatures) Net diffusion stops when the concentration of particles on both sides is equal (if crossing a membrane) or when there is a uniform distribution of particles… this is called equilibrium o When eq ...
... Occurs via random kinetic movement (random movement of particles, which is faster at higher temperatures) Net diffusion stops when the concentration of particles on both sides is equal (if crossing a membrane) or when there is a uniform distribution of particles… this is called equilibrium o When eq ...
Select Acetophenones Modulate Flagellar Motility in Chlamydomonas
... In an attempt to gain insight into the workings of the cilium, we sought to identify novel chemical inhibitors of motility. Accordingly, we screened a chemical library of acetophenones (all obtained from Sigma-Aldrich) for inhibitory effects on ciliary motility. Acetophenones were selected for their ...
... In an attempt to gain insight into the workings of the cilium, we sought to identify novel chemical inhibitors of motility. Accordingly, we screened a chemical library of acetophenones (all obtained from Sigma-Aldrich) for inhibitory effects on ciliary motility. Acetophenones were selected for their ...
The immunological synapse: a focal point for endocytosis and
... The immunological synapse bears striking similarities not only to neurological synapses but also to other structures in which an area of the plasma membrane becomes a focal zone for endo cytosis and exocytosis, in particular during cilia formation and cytokinesis. Immunological synapses, sites of c ...
... The immunological synapse bears striking similarities not only to neurological synapses but also to other structures in which an area of the plasma membrane becomes a focal zone for endo cytosis and exocytosis, in particular during cilia formation and cytokinesis. Immunological synapses, sites of c ...
Tet System Approved FBS
... The problem: Trace tetracycline contaminants in standard Fetal Bovine Serum (FBS) can alter experimental results by enabling background expression in Tet-On® Systems and suppressing maximum expression levels in Tet-Off® systems. The solution: Tet System Approved FBS from Clontech, the only functiona ...
... The problem: Trace tetracycline contaminants in standard Fetal Bovine Serum (FBS) can alter experimental results by enabling background expression in Tet-On® Systems and suppressing maximum expression levels in Tet-Off® systems. The solution: Tet System Approved FBS from Clontech, the only functiona ...
Phosphoinositide Signaling Regulates the Exocyst Complex and
... During migration the reorganization of the actin cytoskeleton, microtubules, and the Golgi apparatus induces polarity in the direction of migration, resulting in polarized membrane trafficking toward the leading edge (Caswell and Norman, 2008; Caswell et al., 2009; Ulrich and Heisenberg, 2009). In d ...
... During migration the reorganization of the actin cytoskeleton, microtubules, and the Golgi apparatus induces polarity in the direction of migration, resulting in polarized membrane trafficking toward the leading edge (Caswell and Norman, 2008; Caswell et al., 2009; Ulrich and Heisenberg, 2009). In d ...
The regulation of Krox-20 expression reveals important steps in the
... heterozygous for the Krox-20 mutation. When placed in standard culture conditions, these Schwann cells quickly lost Krox-20/lacZ expression, with β-galactosidase activity no longer detectable after 24 hours (Fig. 1A,B and data not shown). Since the axon is known to regulate a number of important ste ...
... heterozygous for the Krox-20 mutation. When placed in standard culture conditions, these Schwann cells quickly lost Krox-20/lacZ expression, with β-galactosidase activity no longer detectable after 24 hours (Fig. 1A,B and data not shown). Since the axon is known to regulate a number of important ste ...
Influence of Deformability of Human Red Cells upon Blood Viscosity
... after filtration was markedly reduced. The morphology of the red cell population before filtration showed an increased proportion of crenated disks and crenated spheres with increasing osmolarity. Even at 450 milliosmols/ liter, from 2 to 10% of the cells were seen to be biconcave. After passage thr ...
... after filtration was markedly reduced. The morphology of the red cell population before filtration showed an increased proportion of crenated disks and crenated spheres with increasing osmolarity. Even at 450 milliosmols/ liter, from 2 to 10% of the cells were seen to be biconcave. After passage thr ...
The role of F-cadherin in localizing cells during neural tube
... that subdivide the neural tube into different regions along the neuraxis (Espeseth et al., 1995). One of these boundaries, termed the sulcus limitans, divides the caudal neural tube into a dorsal half, involved in sensory function (the alar plate), and a ventral half involved in motor function (the ...
... that subdivide the neural tube into different regions along the neuraxis (Espeseth et al., 1995). One of these boundaries, termed the sulcus limitans, divides the caudal neural tube into a dorsal half, involved in sensory function (the alar plate), and a ventral half involved in motor function (the ...
Suppression of a mitotic mutant by tRNA
... of scn mutations to suppress the phenotype of cut9-665. The previously identified scn1+ was actually a high copy suppressor for scn1 and scn2 mutations so that it was renamed as scn3+. We will discuss the implication of these unexpected findings on the mitotic defects of dominant tRNA mutations. Mat ...
... of scn mutations to suppress the phenotype of cut9-665. The previously identified scn1+ was actually a high copy suppressor for scn1 and scn2 mutations so that it was renamed as scn3+. We will discuss the implication of these unexpected findings on the mitotic defects of dominant tRNA mutations. Mat ...
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... half of gastrulation should not occur in shaved explants having one layer of deep cells. Nevertheless, some was observed, probably because local regions temporarily become two-layered. The daughters of the single round of cell division before stage 10.5 usually change in shape such that they still o ...
... half of gastrulation should not occur in shaved explants having one layer of deep cells. Nevertheless, some was observed, probably because local regions temporarily become two-layered. The daughters of the single round of cell division before stage 10.5 usually change in shape such that they still o ...
Staying alive Department of Science
... Plastids are organelles that only exist in plant cells. They are found in the cytoplasm of the cell and have a double membrane surrounding them. The number of plastids in a cell varies. This number depends on the changing of environmental conditions that the plant encounters and how the plant adjust ...
... Plastids are organelles that only exist in plant cells. They are found in the cytoplasm of the cell and have a double membrane surrounding them. The number of plastids in a cell varies. This number depends on the changing of environmental conditions that the plant encounters and how the plant adjust ...
Hydrocortisone perturbs the cell proliferation pattern during feather
... decreased in the interplumar areas (compare Rg. 3F and Rg. 3B-D). Serial longitudinal sections showed that more developed rudiments displayed features of E 6.5 feather buds, i.e., unlabeled epidermal placodes and labeled aggregating dermal cells (Fig. 4C). Both low and high magnifications corroborat ...
... decreased in the interplumar areas (compare Rg. 3F and Rg. 3B-D). Serial longitudinal sections showed that more developed rudiments displayed features of E 6.5 feather buds, i.e., unlabeled epidermal placodes and labeled aggregating dermal cells (Fig. 4C). Both low and high magnifications corroborat ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.