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View/Open - Queen Mary University of London
... glycolytic programme, which indeed is essential for effective acquisition of a pluripotent state. These findings suggested that not only are metabolic phenotype and stemness intrinsically linked, but rather cellular metabolism actually controls stemness properties. Thus, it was postulated that activ ...
... glycolytic programme, which indeed is essential for effective acquisition of a pluripotent state. These findings suggested that not only are metabolic phenotype and stemness intrinsically linked, but rather cellular metabolism actually controls stemness properties. Thus, it was postulated that activ ...
Isolation and characterization of an anaerobic benzoate
... and energy source. A Gram-negative motile rodshaped bacterium, which produced gas vacuoles 'during sporulation was isolated. It degraded alcohols, aromatic and n-fatty acids (up to CI8) except for propionate, completely to CO,. Sulfate, sulfite, thiosulfate or elementaj sulfur served as electron acc ...
... and energy source. A Gram-negative motile rodshaped bacterium, which produced gas vacuoles 'during sporulation was isolated. It degraded alcohols, aromatic and n-fatty acids (up to CI8) except for propionate, completely to CO,. Sulfate, sulfite, thiosulfate or elementaj sulfur served as electron acc ...
Motion of red blood cells in a capillary with an - AJP
... shape with changing driving pressures. The cell shape is described by the coordinates [r(t),z(t)] of 200 nodal points fixed in the cell membrane. The system of differential equations is solved for dr/dt, dz/dt, p, and ts by a finite difference method; then the cell shape at the next time step is com ...
... shape with changing driving pressures. The cell shape is described by the coordinates [r(t),z(t)] of 200 nodal points fixed in the cell membrane. The system of differential equations is solved for dr/dt, dz/dt, p, and ts by a finite difference method; then the cell shape at the next time step is com ...
DNA asymmetry in stem cells – immortal or mortal?
... (Yadlapalli et al., 2011) and neocortical precursor cells (Fei and Huttner, 2009) showed that stem cells do not inherit the immortal strands. It should be noted that because BrdU is incorporated into the DNA strand of all the chromosomes, one can only address non-random sister chromatid segregation ...
... (Yadlapalli et al., 2011) and neocortical precursor cells (Fei and Huttner, 2009) showed that stem cells do not inherit the immortal strands. It should be noted that because BrdU is incorporated into the DNA strand of all the chromosomes, one can only address non-random sister chromatid segregation ...
Cell Death Suppressor, Arabidopsis BI
... penetration by Blumeria graminis (Hückelhoven et al., 2003), suggesting that AtBI-1 and Mlo may possess similar functions in cellular defense and cell death modulation. Moreover, it was demonstrated that the barley Mlo protein binds to calmodulin (HvCaM3) and the interaction is related to the contro ...
... penetration by Blumeria graminis (Hückelhoven et al., 2003), suggesting that AtBI-1 and Mlo may possess similar functions in cellular defense and cell death modulation. Moreover, it was demonstrated that the barley Mlo protein binds to calmodulin (HvCaM3) and the interaction is related to the contro ...
Vesicle Trafficking during Somatic Cytokinesis
... during early and/or late stages of cell plate formation. Genetic interference of the endocytic pathway during cytokinesis, using a dominant negative (GDP-locked) form of the Rab5 homolog ARA7, or overexpression of the C-terminal part of clathrin heavy chain inhibited FM4-64 internalization and cause ...
... during early and/or late stages of cell plate formation. Genetic interference of the endocytic pathway during cytokinesis, using a dominant negative (GDP-locked) form of the Rab5 homolog ARA7, or overexpression of the C-terminal part of clathrin heavy chain inhibited FM4-64 internalization and cause ...
PGC specification from epiblast
... gastrulation for PGC formation is still unclear. Microsurgical grafting experiments have indicated that posterior primitive streak cells of 7 dpc embryos grafted orthotopically to the same site in other embryos yield PGCs (Copp et al., 1986). In contrast, lateral ectoderm/mesoderm cells yield somati ...
... gastrulation for PGC formation is still unclear. Microsurgical grafting experiments have indicated that posterior primitive streak cells of 7 dpc embryos grafted orthotopically to the same site in other embryos yield PGCs (Copp et al., 1986). In contrast, lateral ectoderm/mesoderm cells yield somati ...
Perspectives in the Coordinate Regulation of Cell Cycle Events in
... must be under genetic control such that the transcription of the genes appear in an orderly manner. It is likely, for example, that r-proteins operons (rps and rpl) would be transcribed in the order of the binding sequence of the r-proteins onto the nascent rRNA during the ribosome assembly process ...
... must be under genetic control such that the transcription of the genes appear in an orderly manner. It is likely, for example, that r-proteins operons (rps and rpl) would be transcribed in the order of the binding sequence of the r-proteins onto the nascent rRNA during the ribosome assembly process ...
Recent Progress in Research of the Mechanism of Fertilization in
... THE CELL CYCLE OF GAMETES AND FERTILIZATION Cell cycle regulation is important to the growth and development of plants. In the 1950’s, the first examinations of plant gamete nuclei DNA contents were performed, using the Feulgen reaction. Preliminary results lead to the rule of DNA content of male an ...
... THE CELL CYCLE OF GAMETES AND FERTILIZATION Cell cycle regulation is important to the growth and development of plants. In the 1950’s, the first examinations of plant gamete nuclei DNA contents were performed, using the Feulgen reaction. Preliminary results lead to the rule of DNA content of male an ...
Augmin Plays a Critical Role in Organizing the
... complex has been isolated from human cells for its role in centrosome-independent MT generation in spindles and chromosome segregation (Lawo et al., 2009; Uehara et al., 2009; Hutchins et al., 2010). Augmin and the g-tubulin complex appear to interact via the WD40 repeat protein NEDD1/GCP-WD (Zhu et ...
... complex has been isolated from human cells for its role in centrosome-independent MT generation in spindles and chromosome segregation (Lawo et al., 2009; Uehara et al., 2009; Hutchins et al., 2010). Augmin and the g-tubulin complex appear to interact via the WD40 repeat protein NEDD1/GCP-WD (Zhu et ...
Mechanism of Phage-induced Lysis in Pneumococci
... Expression of PAL activity in Dp-1 infected cultures of the autolysin-defective mutant The PAL activity was fully expressed in cwl cultures treated with 5-fluorodeoxyuridine in spite of the fact that the titre of viable progeny phage was suppressed by about 65% (Fig. 4). Addition of streptomycin to ...
... Expression of PAL activity in Dp-1 infected cultures of the autolysin-defective mutant The PAL activity was fully expressed in cwl cultures treated with 5-fluorodeoxyuridine in spite of the fact that the titre of viable progeny phage was suppressed by about 65% (Fig. 4). Addition of streptomycin to ...
Down-Regulation of DNA Topoisomerase IIα Leads to Prolonged
... human topoisomerase II␣ or human topoisomerase II have been extensively characterized (Larsen and Jacquemin-Sablon, 1989; Dereuddre et al., 1997; Khelifa et al., 1999). The cells were maintained in minimal essential medium supplemented with 8% fetal calf serum, 100 units/ml penicillin, and 100 g/m ...
... human topoisomerase II␣ or human topoisomerase II have been extensively characterized (Larsen and Jacquemin-Sablon, 1989; Dereuddre et al., 1997; Khelifa et al., 1999). The cells were maintained in minimal essential medium supplemented with 8% fetal calf serum, 100 units/ml penicillin, and 100 g/m ...
Progressive lineage analysis by cell sorting and culture identifies
... dishes were used throughout this study. No exogenous factors were included in the culture, while 10% fetal calf serum was added. Fig. 2 presents the shift of surface marker expression during 4 days of ES cell culture under this condition. ES cells maintained with LIF in gelatin-coated dishes express ...
... dishes were used throughout this study. No exogenous factors were included in the culture, while 10% fetal calf serum was added. Fig. 2 presents the shift of surface marker expression during 4 days of ES cell culture under this condition. ES cells maintained with LIF in gelatin-coated dishes express ...
Teacher Guide for Amplify Cell Structure and Function Module
... organelle. They can focus on one molecule at a time by hiding all but one molecule. » Simulation Tip: Students can use the molecule panel to hide and show particular molecules; this is a way to reduce the noise in the simulation and allow students to study a particular molecule (students will someti ...
... organelle. They can focus on one molecule at a time by hiding all but one molecule. » Simulation Tip: Students can use the molecule panel to hide and show particular molecules; this is a way to reduce the noise in the simulation and allow students to study a particular molecule (students will someti ...
lithium-metal-polymer batteries: from the electrochemical
... the medium for ionic transfer between the two electrodes. When the electrodes are connected together by an external, electrically conductive circuit, the battery’s chemical reaction is activated at both electrodes, liberating and transporting electrons from one electrode to the other. The cells conv ...
... the medium for ionic transfer between the two electrodes. When the electrodes are connected together by an external, electrically conductive circuit, the battery’s chemical reaction is activated at both electrodes, liberating and transporting electrons from one electrode to the other. The cells conv ...
Cell Cycle - University of Bath
... of the early response genes, which are upregulated following mitogen stimulation of proliferation (Figure 4). Cyclin-dependent phosphorylation is functionally linked to activation and suppression of both p53 and RB-related tumour suppressor genes, which mediate transcriptional events involved with p ...
... of the early response genes, which are upregulated following mitogen stimulation of proliferation (Figure 4). Cyclin-dependent phosphorylation is functionally linked to activation and suppression of both p53 and RB-related tumour suppressor genes, which mediate transcriptional events involved with p ...
Cell-cycle regulation
... is controlled intrinsically by the cell-cycle machinery. The basic components of this machinery are conserved in all eukaryotes. Consequently, findings based on genetics in yeast, biochemistry in frog eggs and tissue culture of mammalian cells have all come together and generated a substantial molec ...
... is controlled intrinsically by the cell-cycle machinery. The basic components of this machinery are conserved in all eukaryotes. Consequently, findings based on genetics in yeast, biochemistry in frog eggs and tissue culture of mammalian cells have all come together and generated a substantial molec ...
Protein Kinase C Activators Inhibit Receptor
... cell. Finally, the closed caveolae open to expose the receptors for another round of folate uptake. Evidence is beginning to accumulate that caveolae are not only a membrane vehicle for potocytosis; they also appear to play an important role in coordinating the receipt and the transmission of cellul ...
... cell. Finally, the closed caveolae open to expose the receptors for another round of folate uptake. Evidence is beginning to accumulate that caveolae are not only a membrane vehicle for potocytosis; they also appear to play an important role in coordinating the receipt and the transmission of cellul ...
Stem Cells – general characteristic and sources
... The method of obtaining pluripotent stem cells from banked embryons, derived by fertilization for clinical purposes, was abandoned because of the fact that banked embryos cells has incompatibility with the potential recipient cells, and therefore embryonic cell lines will differentiate into cells wi ...
... The method of obtaining pluripotent stem cells from banked embryons, derived by fertilization for clinical purposes, was abandoned because of the fact that banked embryos cells has incompatibility with the potential recipient cells, and therefore embryonic cell lines will differentiate into cells wi ...
Production of macrophage activating factors by the mitogen
... supernatants were collected. Thyoglycolate induced, allogenic, peritoneal macrophages were incubated with or without stimulated cell supernatants. Then, the phagocytic activity against inactivated yeast and peroxidase production were measured. Stimulated cell supernatants increased the phagocytic ac ...
... supernatants were collected. Thyoglycolate induced, allogenic, peritoneal macrophages were incubated with or without stimulated cell supernatants. Then, the phagocytic activity against inactivated yeast and peroxidase production were measured. Stimulated cell supernatants increased the phagocytic ac ...
Blm10 binds to preactivated proteasome core particles with open
... 2000). Thus, CP activity is inhibited or latent under physiological conditions. In vitro, latent CP activity is de-repressed by detergent and mild chemical treatments (Wilk & Orlowski, 1983), which influence the equilibrium between the open and closed CP status. In vivo, activators such as the ATPas ...
... 2000). Thus, CP activity is inhibited or latent under physiological conditions. In vitro, latent CP activity is de-repressed by detergent and mild chemical treatments (Wilk & Orlowski, 1983), which influence the equilibrium between the open and closed CP status. In vivo, activators such as the ATPas ...
Transcription Domain-Associated Repair in Human Cells
... is targeted to the transcribed strand in expressed genes. We now report that the previously termed differentiation-associated repair (DAR) depends upon transcription, but not simply upon RNA polymerase II (RNAPII) encountering a lesion: proficient repair of both DNA strands can occur in a part of a ...
... is targeted to the transcribed strand in expressed genes. We now report that the previously termed differentiation-associated repair (DAR) depends upon transcription, but not simply upon RNA polymerase II (RNAPII) encountering a lesion: proficient repair of both DNA strands can occur in a part of a ...
Chapter 3 PDF
... The German scientist Matthias Schleiden also used compound microscopes to study plant tissue. In 1838, he proposed that plants are made of cells. Schleiden discussed the results of his work with another German scientist, Theodor Schwann, who was struck by the structural similarities between plant ce ...
... The German scientist Matthias Schleiden also used compound microscopes to study plant tissue. In 1838, he proposed that plants are made of cells. Schleiden discussed the results of his work with another German scientist, Theodor Schwann, who was struck by the structural similarities between plant ce ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.