![Nance et al gastrulation paper - The Hardin Lab](http://s1.studyres.com/store/data/000097609_1-cc9bf84ca69e92c2146eca22a1f47c3c-300x300.png)
Nance et al gastrulation paper - The Hardin Lab
... ingression is associated with changes in the shape of the ingressing cells and a redistribution of non-muscle myosin. Finally, we present evidence that cell fate, rather than cell position, is the predominant factor regulating ingression, although steric interactions can play an important role. ...
... ingression is associated with changes in the shape of the ingressing cells and a redistribution of non-muscle myosin. Finally, we present evidence that cell fate, rather than cell position, is the predominant factor regulating ingression, although steric interactions can play an important role. ...
Stem cells as a source of insulin
... respectively. Remaining cells were transdifferentiated to insulin-expressing cells by culturing them in serum free medium with GLP1 for three hours and treating them later with ABNG cocktail (Activin-A, betacellulin, nicotinamide and glucose). Insulin expression was significantly enhanced by transfe ...
... respectively. Remaining cells were transdifferentiated to insulin-expressing cells by culturing them in serum free medium with GLP1 for three hours and treating them later with ABNG cocktail (Activin-A, betacellulin, nicotinamide and glucose). Insulin expression was significantly enhanced by transfe ...
Exine dehiscing induces rape microspore polarity
... (Tian and Sun, 2003; Tang et al., 2006). Further studies indicated that the first division of the EDMs, unlike that in intact microspores, could be asymmetric. It was also reported that after mild heat stress treatment, cultured microspores could develop into embryos with a suspensor after slightly ...
... (Tian and Sun, 2003; Tang et al., 2006). Further studies indicated that the first division of the EDMs, unlike that in intact microspores, could be asymmetric. It was also reported that after mild heat stress treatment, cultured microspores could develop into embryos with a suspensor after slightly ...
Human Anatomy and Histology course Lecturer: Anna Barlasov PhD
... Description: Consists of blood plasma (55%) and formed elements (45%): red blood cells (erythrocytes), white blood cells (leukocytes), and platelets (thrombocytes). Location: Within blood vessels (arteries, arterioles, capillaries, venules, and veins) and within the chambers of the heart. Function: ...
... Description: Consists of blood plasma (55%) and formed elements (45%): red blood cells (erythrocytes), white blood cells (leukocytes), and platelets (thrombocytes). Location: Within blood vessels (arteries, arterioles, capillaries, venules, and veins) and within the chambers of the heart. Function: ...
PD-1 promotes immune exhaustion by inducing antiviral T cell
... versus persistent infection. (A) TPM was used to examine the dynamics of CD8+ P14 (red) and CD4+ SMARTA (green) T cells in the spleen after an acute (Arm) or a persistent (CL13) infection (n = 5 mice). Representative 3D reconstructions of two-photon z stacks are shown for infected mice at day 7 afte ...
... versus persistent infection. (A) TPM was used to examine the dynamics of CD8+ P14 (red) and CD4+ SMARTA (green) T cells in the spleen after an acute (Arm) or a persistent (CL13) infection (n = 5 mice). Representative 3D reconstructions of two-photon z stacks are shown for infected mice at day 7 afte ...
Phenotypic Modulation of Smooth Muscle Cells
... siRNA enhances myocardin activity and SMC differentiation. We conclude that signal-dependent interaction of Foxo4 with myocardin couples extracellular signals with the transcriptional program for SMC differentiation. Introduction Unlike skeletal and cardiac muscle cells, smooth muscle cells (SMCs) d ...
... siRNA enhances myocardin activity and SMC differentiation. We conclude that signal-dependent interaction of Foxo4 with myocardin couples extracellular signals with the transcriptional program for SMC differentiation. Introduction Unlike skeletal and cardiac muscle cells, smooth muscle cells (SMCs) d ...
Teacher support material
... There are more activities than time to put them into practice. So teachers have to choose which ones they are going to use. The aim of those activities is to give teachers different ideas on how to recap everything learnt by pupils and let them try those ones more suitable for their pupils. Some of ...
... There are more activities than time to put them into practice. So teachers have to choose which ones they are going to use. The aim of those activities is to give teachers different ideas on how to recap everything learnt by pupils and let them try those ones more suitable for their pupils. Some of ...
Signaling-dependent immobilization of acylated proteins in the inner
... Lyn directs myristoylation and palmitoylation of the chimera, which targets the fluorescent protein to the inner monolayer of the plasmalemma (Teruel et al., 1999). Transverse (x vs. y; Fig. 1 A) and sagittal (x vs. z; Fig. 1 B) sections of the cells confirm that PM-GFP is largely plasmalemmal, alth ...
... Lyn directs myristoylation and palmitoylation of the chimera, which targets the fluorescent protein to the inner monolayer of the plasmalemma (Teruel et al., 1999). Transverse (x vs. y; Fig. 1 A) and sagittal (x vs. z; Fig. 1 B) sections of the cells confirm that PM-GFP is largely plasmalemmal, alth ...
Anatomy of Cells
... other anatomical structures, cells exhibit a particular size or form because they are intended to perform a certain activity. A nerve cell, for example, may have threadlike extensions over a meter in length! Such a cell is ideally suited to transmit nervous impulses from one area of the body to anot ...
... other anatomical structures, cells exhibit a particular size or form because they are intended to perform a certain activity. A nerve cell, for example, may have threadlike extensions over a meter in length! Such a cell is ideally suited to transmit nervous impulses from one area of the body to anot ...
Macromolecular biophysics of the plant cell wall: Concepts and
... Much of this fine detail is lost when cell-wall polymers are extracted into solution, as is necessary before they can be examined by the classical techniques of polysaccharide, protein and lignin chemistry. There is therefore an important role for spectroscopic techniques that can be used to examine ...
... Much of this fine detail is lost when cell-wall polymers are extracted into solution, as is necessary before they can be examined by the classical techniques of polysaccharide, protein and lignin chemistry. There is therefore an important role for spectroscopic techniques that can be used to examine ...
Polycystin-2 functions as an intracellular calcium release channel.
... lost after carboxy-terminal truncation or by the introduction of a disease-causing missense mutation. These data suggest that polycystin-2 functions as a calcium-activated intracellular calcium release channel in vivo and that polycystic kidney disease results from the loss of a regulated intracellu ...
... lost after carboxy-terminal truncation or by the introduction of a disease-causing missense mutation. These data suggest that polycystin-2 functions as a calcium-activated intracellular calcium release channel in vivo and that polycystic kidney disease results from the loss of a regulated intracellu ...
Cell Structure Tumor Microenvironment
... “is the functional and smallest unit in every living organism ” • In 1665, the cell was discovered by Robert Hooke. • In 1839, the cell theroy was developed by Matthias J. Schleiden and Theodor Schwann (Ref: The Molecular Probes® Handbook-11th Edition, 2010, Invitrogen) ...
... “is the functional and smallest unit in every living organism ” • In 1665, the cell was discovered by Robert Hooke. • In 1839, the cell theroy was developed by Matthias J. Schleiden and Theodor Schwann (Ref: The Molecular Probes® Handbook-11th Edition, 2010, Invitrogen) ...
Protista: nature`s superstore
... A Volvox colony is composed of hundreds, or thousands, of flagellated cells arranged in a single layer forming a hollow ball-shaped structure. The cells are connected by strands of cytoplasm, and the flagella of individual cells face outward. The flagella can beat in a coordinated fashion, spinning ...
... A Volvox colony is composed of hundreds, or thousands, of flagellated cells arranged in a single layer forming a hollow ball-shaped structure. The cells are connected by strands of cytoplasm, and the flagella of individual cells face outward. The flagella can beat in a coordinated fashion, spinning ...
Ciprofloxacin Hydrochloride
... gram negative bacteria1, and is most well known for its effectiveness against mycoplasma. Ciprofloxacin HCl works by interfering with the bacterial enzyme DNA gyrase, an enzyme necessary for bacterial synthesis, replication, and transcription in both the active and non-active growth phases of the ba ...
... gram negative bacteria1, and is most well known for its effectiveness against mycoplasma. Ciprofloxacin HCl works by interfering with the bacterial enzyme DNA gyrase, an enzyme necessary for bacterial synthesis, replication, and transcription in both the active and non-active growth phases of the ba ...
Gene nuc2 - The Journal of Cell Biology
... 4 h and examined by DAPI stain for whether chromosomes of ts mutant cells remain condensed without separation. In the wild-type S. pombe, chromosomes separate after condensation (e.g., Toda et al., 1981). Therefore, if mutations specifically prevent anaphase, mutant cells should uniformly arrest, sh ...
... 4 h and examined by DAPI stain for whether chromosomes of ts mutant cells remain condensed without separation. In the wild-type S. pombe, chromosomes separate after condensation (e.g., Toda et al., 1981). Therefore, if mutations specifically prevent anaphase, mutant cells should uniformly arrest, sh ...
of Limb Morphogenesis in a Model System
... as are chondrocytes from stage 30, or morphogenetic expression, as are stage 19 cells, they must contain either some components of both cell types, or an intermediate cell type with the properties of neither. If this population contained a subpopulation of stage 19 cells, then morphogenesis should r ...
... as are chondrocytes from stage 30, or morphogenetic expression, as are stage 19 cells, they must contain either some components of both cell types, or an intermediate cell type with the properties of neither. If this population contained a subpopulation of stage 19 cells, then morphogenesis should r ...
Embryonic stem cells form an organized, functional - AJP
... undifferentiated ES cells containing both vector constructs (minKlacZ and cGATA6-ERFP) were generated, they were differentiated using the “hanging-drop” method as previously described (32). Briefly, 20-l drops containing 200 ES cells each in differentiation medium (growth medium without leukemia in ...
... undifferentiated ES cells containing both vector constructs (minKlacZ and cGATA6-ERFP) were generated, they were differentiated using the “hanging-drop” method as previously described (32). Briefly, 20-l drops containing 200 ES cells each in differentiation medium (growth medium without leukemia in ...
Plant Cell Walls: Basics of Structure, Chemistry, Accessibility and the
... larger and longer fibrils [9]. The cellulose microfibrils are hydrophobic and can be highly crystalline, features that contribute greatly to the recalcitrance of biomass. For example, the 100 crystal face of a microfibril is more hydrophobic than the other faces and selectively binds the CBD (cellul ...
... larger and longer fibrils [9]. The cellulose microfibrils are hydrophobic and can be highly crystalline, features that contribute greatly to the recalcitrance of biomass. For example, the 100 crystal face of a microfibril is more hydrophobic than the other faces and selectively binds the CBD (cellul ...
Hungry for Power: Elimination of Mitochondria by Mitophagy
... It is often said that nothing in life is free, and the role played by the mitochondrion in eukaryotic cells is no exception. Through oxidative phosphorylation, mitochondria provide large amounts of energy to the cell, which has made possible an energy-intensive way of life and, ultimately, the evolu ...
... It is often said that nothing in life is free, and the role played by the mitochondrion in eukaryotic cells is no exception. Through oxidative phosphorylation, mitochondria provide large amounts of energy to the cell, which has made possible an energy-intensive way of life and, ultimately, the evolu ...
Unit 1 Cells and System
... • Tissue is a group of cells that have the same structure and function. • Four types of body tissue: Muscle tissue Assists in body movement Nerve tissue Transfers signals to and from brain Connective tissue Holds together and supports other tissue Epithelial tissue Covers the surface ...
... • Tissue is a group of cells that have the same structure and function. • Four types of body tissue: Muscle tissue Assists in body movement Nerve tissue Transfers signals to and from brain Connective tissue Holds together and supports other tissue Epithelial tissue Covers the surface ...
Targets of Oncogenes and Tumor Suppressors: Key for
... tumor cells. Activities of sequential cyclin-dependent kinases are the motor of the cell cycle [16] (Fig. 1). Each cyclin-dependent kinase (Cdk) is a catalytic subunit of the holoenzyme complex that requires the presence of the activating subunit, cyclin, for manifestation of catalytic activity. R ...
... tumor cells. Activities of sequential cyclin-dependent kinases are the motor of the cell cycle [16] (Fig. 1). Each cyclin-dependent kinase (Cdk) is a catalytic subunit of the holoenzyme complex that requires the presence of the activating subunit, cyclin, for manifestation of catalytic activity. R ...
N-terminal and C-terminal plasma membrane
... (CHO-2B cells) were maintained as described previously [25]. CHO-2B cells were stably transfected with either wild-type cPLA2/pcDNA3, myc-cPLA2/pcDNA3, Lck-cPLA2/pcDNA3 or cPLA2-Ras/pcDNA3 using the calcium phosphate precipitation method. Resistant clones were selected in medium supplemented with 0. ...
... (CHO-2B cells) were maintained as described previously [25]. CHO-2B cells were stably transfected with either wild-type cPLA2/pcDNA3, myc-cPLA2/pcDNA3, Lck-cPLA2/pcDNA3 or cPLA2-Ras/pcDNA3 using the calcium phosphate precipitation method. Resistant clones were selected in medium supplemented with 0. ...
Formation of the leading edge boundary
... Noselli, 1997; Martin-Blanco et al., 1998; Ring and Martinez Arias, 1993). The transgenic insert, pucE69, leads to loss of Puc function, but in the heterozygous state, expresses nuclear localized β-galactosidase (β-gal) in a pattern restricted to the LE cells. These heterozygous animals appear wild ...
... Noselli, 1997; Martin-Blanco et al., 1998; Ring and Martinez Arias, 1993). The transgenic insert, pucE69, leads to loss of Puc function, but in the heterozygous state, expresses nuclear localized β-galactosidase (β-gal) in a pattern restricted to the LE cells. These heterozygous animals appear wild ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.