Direct Visualization by Cryo-EM of the Mycobacterial Capsular
... antisera against known capsular components was performed. Whole bacteria were immobilized on EM grids and incubated with specific antibodies and gold probes. This method also allowed us to visualize and quantify the different components. Since a-glucan is described to be the major capsular polysacch ...
... antisera against known capsular components was performed. Whole bacteria were immobilized on EM grids and incubated with specific antibodies and gold probes. This method also allowed us to visualize and quantify the different components. Since a-glucan is described to be the major capsular polysacch ...
Newly Synthesized Catalytic and Regulatory Components of
... postnatal) were excised initially into enriched Ll5 (Gibco) plating medium (prepared as described by Hawrot and Patterson, 1979) without addedglucose. Ganglia were desheathed and transferred to final plating medium containing 50 rllml 30% glucose (0.75% final concentration). Ganglia could be stored ...
... postnatal) were excised initially into enriched Ll5 (Gibco) plating medium (prepared as described by Hawrot and Patterson, 1979) without addedglucose. Ganglia were desheathed and transferred to final plating medium containing 50 rllml 30% glucose (0.75% final concentration). Ganglia could be stored ...
HYPERTROPHY OF THE HUMAN HEART AT THE LEVEL OF FINE
... solution (13) so that myofibrils could be distinguished with certainty from other cell constituents. Photomicrographs were taken through an apochromatic objective with a numerical aperture of 1.4. Counts of the number of myofibrils per cell were made on suitable enlargements of cross-sectional image ...
... solution (13) so that myofibrils could be distinguished with certainty from other cell constituents. Photomicrographs were taken through an apochromatic objective with a numerical aperture of 1.4. Counts of the number of myofibrils per cell were made on suitable enlargements of cross-sectional image ...
Principles of Bacterial Toxin Entry
... Members of a second group of AB toxins access cytosolic substrates by exploiting membrane transport complexes located in the endoplasmic reticulum (ER) (Fig. 3). Upon entering their target cells, these toxins exploit one of several “retrograde” trafficking pathways destined for the ER. Within the lu ...
... Members of a second group of AB toxins access cytosolic substrates by exploiting membrane transport complexes located in the endoplasmic reticulum (ER) (Fig. 3). Upon entering their target cells, these toxins exploit one of several “retrograde” trafficking pathways destined for the ER. Within the lu ...
Identification of novel MYO18A interaction partners - HAL
... Unconventional myosins do not form the structure of myofibrils, however, they have been shown to play important roles in the regulation of a wide range of cellular functions, including cell migration, intracellular trafficking, adhesion and cytokinesis10, although their implication in muscle cell fu ...
... Unconventional myosins do not form the structure of myofibrils, however, they have been shown to play important roles in the regulation of a wide range of cellular functions, including cell migration, intracellular trafficking, adhesion and cytokinesis10, although their implication in muscle cell fu ...
The Arabidopsis sku6-spiral1 gene encodes a plus end
... (I) and (J) Wild-type (I) and spr1-6 (J) plants growing in the greenhouse. In (J), note the two spr1-6 leaves (indicated by arrows) that are not laying flat because of right-handed twisted petioles; the top right leaf is sideways, whereas the bottom left leaf is upside down. (K) and (L) Wild-type (K ...
... (I) and (J) Wild-type (I) and spr1-6 (J) plants growing in the greenhouse. In (J), note the two spr1-6 leaves (indicated by arrows) that are not laying flat because of right-handed twisted petioles; the top right leaf is sideways, whereas the bottom left leaf is upside down. (K) and (L) Wild-type (K ...
Mechanistic investigation into the actions of taurine on beta cells
... Taurine (2-aminoethanesulphonic acid) is a semi-essential amino acid, which is not incorporated into proteins. In mammalian tissues, taurine is ubiquitous and is the most abundant free amino acid in the heart, retina, skeletal muscle, brain, and leukocytes, exhibiting multiple functions and tissue-p ...
... Taurine (2-aminoethanesulphonic acid) is a semi-essential amino acid, which is not incorporated into proteins. In mammalian tissues, taurine is ubiquitous and is the most abundant free amino acid in the heart, retina, skeletal muscle, brain, and leukocytes, exhibiting multiple functions and tissue-p ...
Resident and infiltrating immune cells in the uveal tract in the
... (DC). This peptide-MHC class II complex interacts with the T-cell receptor14 and is aided by accessory cell adhesion and co-stimulatory molecules.1516 Although it is well known that uveitis is T cell-mediated, the cell(s) within the eye responsible for presentation of retinal autoantigens and induct ...
... (DC). This peptide-MHC class II complex interacts with the T-cell receptor14 and is aided by accessory cell adhesion and co-stimulatory molecules.1516 Although it is well known that uveitis is T cell-mediated, the cell(s) within the eye responsible for presentation of retinal autoantigens and induct ...
Biodiversity
... Viruses clear out the waste in our bodies when they are not severe such as the common cold. Viruses like bacteriophages kill spectrums on bacteria that is harmful to the human body. Viruses can be helpful to humans by helping us find cures to diseases, but their main role in nature is causing dise ...
... Viruses clear out the waste in our bodies when they are not severe such as the common cold. Viruses like bacteriophages kill spectrums on bacteria that is harmful to the human body. Viruses can be helpful to humans by helping us find cures to diseases, but their main role in nature is causing dise ...
Construction of high-density bacterial colony arrays and
... cells are more fragile and heat-sensitive than most other E. coli strains. However, our results showed that although the temperature of the heated plate can reach 200– 300jC, the system still reliably delivered viable cells onto the agarcoated surfaces. One possible explanation for their survival i ...
... cells are more fragile and heat-sensitive than most other E. coli strains. However, our results showed that although the temperature of the heated plate can reach 200– 300jC, the system still reliably delivered viable cells onto the agarcoated surfaces. One possible explanation for their survival i ...
Syndecan-1 regulates αvß5 integrin activity in B82L fibroblasts
... on antibodies specific to syndecan-2 (Sdc2) (Park et al., 2002). When grown on serum-coated substrata, these cells exhibit a highly spread morphology, but revert to a rounded morphology when treated with recombinant Sdc2 ectodomain, suggesting an important role for this region of the core protein. S ...
... on antibodies specific to syndecan-2 (Sdc2) (Park et al., 2002). When grown on serum-coated substrata, these cells exhibit a highly spread morphology, but revert to a rounded morphology when treated with recombinant Sdc2 ectodomain, suggesting an important role for this region of the core protein. S ...
The Cytology of the Gram-positive Cocci
... bore a close resemblance, in their minute structure, to long-chained streptococci. Well-marked transverse septa were observed (PI. 2, fig. 8 ) their position being indicated in the Giemsa preparations by an unstained bar across the middle of the coccus (Pl. 2, fig. 9). Unlike the streptococci, howev ...
... bore a close resemblance, in their minute structure, to long-chained streptococci. Well-marked transverse septa were observed (PI. 2, fig. 8 ) their position being indicated in the Giemsa preparations by an unstained bar across the middle of the coccus (Pl. 2, fig. 9). Unlike the streptococci, howev ...
30Reasons Why You Need a Shot a Day!
... damage the DNA of cells! Ultimately this scavenging increases our ability to live healthier, longer lives. 14) Ningxia Wolfberries free radical scavengers help to strengthen and support the cell walls. This supports efficient transport of flavonoid nutrients across the cell membrane or wall. 15) Str ...
... damage the DNA of cells! Ultimately this scavenging increases our ability to live healthier, longer lives. 14) Ningxia Wolfberries free radical scavengers help to strengthen and support the cell walls. This supports efficient transport of flavonoid nutrients across the cell membrane or wall. 15) Str ...
Movements and stepwise fusion of endodermal precursor cells in
... lineage tracers leak from one macromere into the others during stages 7–8. We have now found that the fusion of the macromeres occurs in a two-step process with a set time course. The first step is the fusion of A′′′ and B′′′ to form a cell we designate as A/B. When we injected β-galactosidase into ...
... lineage tracers leak from one macromere into the others during stages 7–8. We have now found that the fusion of the macromeres occurs in a two-step process with a set time course. The first step is the fusion of A′′′ and B′′′ to form a cell we designate as A/B. When we injected β-galactosidase into ...
IP3R-mediated Ca2+ release regulates protein metabolism in
... itprku might therefore be a strategy to accumulate sufficient protein, and also explain why it does not result in increased body size. Abnormal protein/TAG ratios suggested perturbed insulin signaling in itprku. We therefore measured transcript levels of Drosophila insulin-like peptides (dILPs) 2, 3 ...
... itprku might therefore be a strategy to accumulate sufficient protein, and also explain why it does not result in increased body size. Abnormal protein/TAG ratios suggested perturbed insulin signaling in itprku. We therefore measured transcript levels of Drosophila insulin-like peptides (dILPs) 2, 3 ...
Primordial germ cells migration: morphological and molecular aspects
... embryology is exactly which mechanisms maintain the PGCs during their migration as well as which factors control PGC migration and homing within the genital ridges. This process requires integrated signals involving contact of PGCs with extracellular matrix molecules and cellular substrates or repul ...
... embryology is exactly which mechanisms maintain the PGCs during their migration as well as which factors control PGC migration and homing within the genital ridges. This process requires integrated signals involving contact of PGCs with extracellular matrix molecules and cellular substrates or repul ...
A differential requirement for SUMOylation in proliferating and non
... Animal development is achieved through the coordination of cell growth, cell division and cell death. During the early embryogenesis of Drosophila melanogaster, cells divide rapidly, which is facilitated by maternal deposition of components of the cell cycle machinery (Foe et al., 1993). After the b ...
... Animal development is achieved through the coordination of cell growth, cell division and cell death. During the early embryogenesis of Drosophila melanogaster, cells divide rapidly, which is facilitated by maternal deposition of components of the cell cycle machinery (Foe et al., 1993). After the b ...
Project Details - School of Biomedical Sciences
... Taurine (2-aminoethanesulphonic acid) is a semi-essential amino acid, which is not incorporated into proteins. In mammalian tissues, taurine is ubiquitous and is the most abundant free amino acid in the heart, retina, skeletal muscle, brain, and leukocytes, exhibiting multiple functions and tissue-p ...
... Taurine (2-aminoethanesulphonic acid) is a semi-essential amino acid, which is not incorporated into proteins. In mammalian tissues, taurine is ubiquitous and is the most abundant free amino acid in the heart, retina, skeletal muscle, brain, and leukocytes, exhibiting multiple functions and tissue-p ...
Sporangiophores' Technique Study Transpiration Pressure Probe
... The following procedure was used for the experiments conducted. Initially, a large stage III sporangiophore was carefully removed from the mycelium with tweezers (plucked), and transferred to a small chamber. Plucked stage IV sporangiophores continue to grow at almost normal rates after their base i ...
... The following procedure was used for the experiments conducted. Initially, a large stage III sporangiophore was carefully removed from the mycelium with tweezers (plucked), and transferred to a small chamber. Plucked stage IV sporangiophores continue to grow at almost normal rates after their base i ...
Diefenbach, A., J.K. Hsia, M.Y. Hsiung, and D.H. Raulet. 2003. A novel ligand for the NKG2D receptor activates NK cells and macrophages and induces tumor immunity. Eur J Immunol 33:381-391.
... NK cells also express a variety of stimulatory receptors. Until recently the significance of these receptors was unclear mainly because the specificity of these receptors was unknown. One of these receptors is NKG2D. This is a homodimeric C-type lectin-like protein that is expressed by all NK cells, ...
... NK cells also express a variety of stimulatory receptors. Until recently the significance of these receptors was unclear mainly because the specificity of these receptors was unknown. One of these receptors is NKG2D. This is a homodimeric C-type lectin-like protein that is expressed by all NK cells, ...
Gumbo JR and Cloete TE
... cell death upon exposure to B. mycoides i.e. cell membrane lysis and shadowing of algal cells leading to photo-inhibition. There were ultra-structural changes that occurred in bacteria treated M. aeruginosa cells. SEM images showed swollen M. aeruginosa cells due to cell membrane damage and increase ...
... cell death upon exposure to B. mycoides i.e. cell membrane lysis and shadowing of algal cells leading to photo-inhibition. There were ultra-structural changes that occurred in bacteria treated M. aeruginosa cells. SEM images showed swollen M. aeruginosa cells due to cell membrane damage and increase ...
Essays in Biochemistry Volume 39 Chapter 1
... The morphology of vertebrate and invertebrate cells undergoing apoptosis was similar regardless of the initial apoptotic signal. This suggested that a common apoptotic pathway that is activated in response to many different initial stimuli exists in cells. Previous studies with C. elegans in the 198 ...
... The morphology of vertebrate and invertebrate cells undergoing apoptosis was similar regardless of the initial apoptotic signal. This suggested that a common apoptotic pathway that is activated in response to many different initial stimuli exists in cells. Previous studies with C. elegans in the 198 ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.