Collagen Binding Proteins Derived from the Embryonic Fibroblast
... affinity chromatography. Certain of these proteins (Mr = 120,000, 90,000 and 47,000) were specifically eluted from collagen affinity columns by synthetic peptides containing the amino acid sequence arginyl-glycyl-aspartic acid (RGD). These data show that a number of collagen binding proteins exist o ...
... affinity chromatography. Certain of these proteins (Mr = 120,000, 90,000 and 47,000) were specifically eluted from collagen affinity columns by synthetic peptides containing the amino acid sequence arginyl-glycyl-aspartic acid (RGD). These data show that a number of collagen binding proteins exist o ...
Encapsulated Choroid Plexus Epithelial Cells Actively Protect
... Before transfection, CPECs were maintained in culture medium supplemented with 10 % fetal bovine serum and allowed to reach 60–70 % confluency. Transfection was carried out using the calcium phosphate method (Calphos transfection kit, Clontech). The medium was replaced 4 h before the transfection. T ...
... Before transfection, CPECs were maintained in culture medium supplemented with 10 % fetal bovine serum and allowed to reach 60–70 % confluency. Transfection was carried out using the calcium phosphate method (Calphos transfection kit, Clontech). The medium was replaced 4 h before the transfection. T ...
Distribution, function, and prognostic value of cytotoxic T lymphocytes
... blood or from lymph node tissue were cocultured with either EBVtransformed B cells (JY)23 or with autologous FL B cells, respectively, which were either unpulsed or pulsed with bacterial superantigen cocktail (SAg, 100 ng/mL TSST-1, SEC 1, SEB, SEE). After 1 hour of culture, cells were stained with ...
... blood or from lymph node tissue were cocultured with either EBVtransformed B cells (JY)23 or with autologous FL B cells, respectively, which were either unpulsed or pulsed with bacterial superantigen cocktail (SAg, 100 ng/mL TSST-1, SEC 1, SEB, SEE). After 1 hour of culture, cells were stained with ...
PDF
... Fig. 1. Characterization of the long isoforms (UNC-53L) of unc-53. (A) Structure of the unc-53 gene. The start of the various UNC-53L and UNC-53S isoforms are indicated by arrows. The promoter for UNC-53SA is between exons 5 and 8, and the promoter for UNC-53SB is located between exons 8 and 13 (Cho ...
... Fig. 1. Characterization of the long isoforms (UNC-53L) of unc-53. (A) Structure of the unc-53 gene. The start of the various UNC-53L and UNC-53S isoforms are indicated by arrows. The promoter for UNC-53SA is between exons 5 and 8, and the promoter for UNC-53SB is located between exons 8 and 13 (Cho ...
Prokaryotes
... variety of ways. In fact, prokaryotes have just about every possible type of metabolism. They may get energy from light (photo) or chemical compounds (chemo). They may get carbon from carbon dioxide (autotroph) or other living things (heterotroph). Table below shows all the possible types of metabol ...
... variety of ways. In fact, prokaryotes have just about every possible type of metabolism. They may get energy from light (photo) or chemical compounds (chemo). They may get carbon from carbon dioxide (autotroph) or other living things (heterotroph). Table below shows all the possible types of metabol ...
by Permanent DNA Rearrangements The Ontogeny and Fate of NK
... a discontinuous Percoll gradient (20). Before the sorting of specific NK cell subsets, BM or spleen cells were enriched using the NK cell isolation kit (Miltenyi Biotec). Cells were incubated with primary Abs for 20 min, washed three times, incubated with streptavidin reagents for 15 min, and then w ...
... a discontinuous Percoll gradient (20). Before the sorting of specific NK cell subsets, BM or spleen cells were enriched using the NK cell isolation kit (Miltenyi Biotec). Cells were incubated with primary Abs for 20 min, washed three times, incubated with streptavidin reagents for 15 min, and then w ...
division plane orientation in plant cells
... While the centering of the nucleus by a MT-based mechanism appears to be an important part of the division plane selection process in some cells, nuclear position alone cannot be sufficient to determine the division plane. For example, an elongated cell may divide symmetrically in either transverse ...
... While the centering of the nucleus by a MT-based mechanism appears to be an important part of the division plane selection process in some cells, nuclear position alone cannot be sufficient to determine the division plane. For example, an elongated cell may divide symmetrically in either transverse ...
Cloning, Expression in Escherichia coli, and Characterization of
... When tested for utilization of carbon sources, Azoarcus cells were grown on KW plates for 4 days, scraped off, and washed twice in saline. They were used as an inoculum (at 6 [ig of protein per ml) for test cultures in liquid KW medium without yeast extract (25 ml in 125-ml Erlenmeyer flasks) and we ...
... When tested for utilization of carbon sources, Azoarcus cells were grown on KW plates for 4 days, scraped off, and washed twice in saline. They were used as an inoculum (at 6 [ig of protein per ml) for test cultures in liquid KW medium without yeast extract (25 ml in 125-ml Erlenmeyer flasks) and we ...
Diefenbach, A., and D.H. Raulet. 2002. The innate immune response to tumors and its role in the induction of T cell immunity. Immunological Reviews 188:9-21.
... complex combinatorial repertoire of NK specificities for MHC class I molecules (54). The inhibitory receptor repertoire is shaped by an education mechanism that guarantees that NK cells are self-tolerant but are unleashed against target cells that down-regulate some or all self-class I molecules (54 ...
... complex combinatorial repertoire of NK specificities for MHC class I molecules (54). The inhibitory receptor repertoire is shaped by an education mechanism that guarantees that NK cells are self-tolerant but are unleashed against target cells that down-regulate some or all self-class I molecules (54 ...
Section 20–1 The Kingdom Protista (pages 497–498)
... you have read. Look carefully at Figure 20–1 on page 497. What important idea do these photographs communicate? Do your work on a separate sheet of paper. Students should infer that the main idea these photographs communicate is that the kingdom Protista consists of an extremely diverse group of org ...
... you have read. Look carefully at Figure 20–1 on page 497. What important idea do these photographs communicate? Do your work on a separate sheet of paper. Students should infer that the main idea these photographs communicate is that the kingdom Protista consists of an extremely diverse group of org ...
THE PLANT CELL CYCLE Walter Dewitte and James A.H. Murray
... certain cellular aspects, including the intracellular localization of proteins, can be studied in individual cells and therefore do not require consistent timing of division between cells, the biochemical and molecular analysis of cell division is predicated on the availability of cell systems that ...
... certain cellular aspects, including the intracellular localization of proteins, can be studied in individual cells and therefore do not require consistent timing of division between cells, the biochemical and molecular analysis of cell division is predicated on the availability of cell systems that ...
PITT pGLO Transformation Lab Protocol
... of a gene by removing the repressor protein from the gene’s specific “ara” promoter indicator medium a growth medium that causes some cells to appear differently than other cells, indicating the presence or absence of certain traits ...
... of a gene by removing the repressor protein from the gene’s specific “ara” promoter indicator medium a growth medium that causes some cells to appear differently than other cells, indicating the presence or absence of certain traits ...
The Molecular Basis of Self-Avoidance
... another. In addition to these exons, a pair of alternatively spliced exons encode different transmembrane domains and some adjacent sequences; these differentially control localization of Dscam1 proteins to axons and dendrites (Wang et al. 2004, Shi et al. 2007, Yang et al. 2008). Finally, alternati ...
... another. In addition to these exons, a pair of alternatively spliced exons encode different transmembrane domains and some adjacent sequences; these differentially control localization of Dscam1 proteins to axons and dendrites (Wang et al. 2004, Shi et al. 2007, Yang et al. 2008). Finally, alternati ...
Connective Tissue
... • Local blood vessels become more permeable, allowing white blood cells, fluid, clotting proteins and other plasma proteins to seep into the injured area. • Clotting occurs; surface dries and forms a scab. ...
... • Local blood vessels become more permeable, allowing white blood cells, fluid, clotting proteins and other plasma proteins to seep into the injured area. • Clotting occurs; surface dries and forms a scab. ...
View/Open - Digital Collections
... form to the anomocytic type (e-g., Fig. 14, 16?), and often contain dark contents (Fig. 2 1). They but in others there is a ring of weakly special- show numerous similarities in size, shape, and ized cells and hence the stomata might be clas- appearance of contents to oil cells illustrated sified as ...
... form to the anomocytic type (e-g., Fig. 14, 16?), and often contain dark contents (Fig. 2 1). They but in others there is a ring of weakly special- show numerous similarities in size, shape, and ized cells and hence the stomata might be clas- appearance of contents to oil cells illustrated sified as ...
Document
... During the M phase of cell division, the chromatids are condensed by proteins called? (a) cohesins (b) condensins (c) cytochromes (d) selectins (e) None of the above ...
... During the M phase of cell division, the chromatids are condensed by proteins called? (a) cohesins (b) condensins (c) cytochromes (d) selectins (e) None of the above ...
Mary Ann Thompson Arildsen, M
... focused on teaching, and the other will be focused on instrumentation. We are very fortunate to have 4 strong internal candidates to choose from. I plan to work with the teaching MTIII to strengthen the bi-annual proficiency testing of the technologists, and I plan to work with the instrumentation M ...
... focused on teaching, and the other will be focused on instrumentation. We are very fortunate to have 4 strong internal candidates to choose from. I plan to work with the teaching MTIII to strengthen the bi-annual proficiency testing of the technologists, and I plan to work with the instrumentation M ...
Pseudolaric Acid B Induced Cell Cycle Arrest, Au
... stage, then autophagy, lastly senescence. We could observe that from 12 h, PAB begun to induce obvious G2/M cell cycle arrest, but there was no subdiploid peak of apoptosis marker. In control group, the G2/M cell cycle arrest ratio was 12.5%, at 12 h after PAB treatment it was 52.79%, at 24 h it was ...
... stage, then autophagy, lastly senescence. We could observe that from 12 h, PAB begun to induce obvious G2/M cell cycle arrest, but there was no subdiploid peak of apoptosis marker. In control group, the G2/M cell cycle arrest ratio was 12.5%, at 12 h after PAB treatment it was 52.79%, at 24 h it was ...
Cortical cell fate specification
... made using a vibratome, and 4-6 slices were then placed on poly-llysine- and laminin-coated membrane inserts in 6-well plates using an air-interface protocol (Polleux et al., 1998; Polleux et al., 2000). In each experiment, approximately 105 dissociated GFP cells were plated per well. The medium use ...
... made using a vibratome, and 4-6 slices were then placed on poly-llysine- and laminin-coated membrane inserts in 6-well plates using an air-interface protocol (Polleux et al., 1998; Polleux et al., 2000). In each experiment, approximately 105 dissociated GFP cells were plated per well. The medium use ...
Article
... levels of the ITO (–4.7 eV) and the Nc-TiO2 (–4.4 eV) are raised to a level higher than the energy of the counter electrode, probably by pinning with the excited-state of EB. The Nc-TiO 2 film, in addition to its use as a matrix for the growth of PAni, participates in the light absorption (in the sh ...
... levels of the ITO (–4.7 eV) and the Nc-TiO2 (–4.4 eV) are raised to a level higher than the energy of the counter electrode, probably by pinning with the excited-state of EB. The Nc-TiO 2 film, in addition to its use as a matrix for the growth of PAni, participates in the light absorption (in the sh ...
Chapter 28 Animal Tissue and Organ Systems
... Cultured Skin Products • Skin is the only organ that is grown artificially for widespread medical uses – Cells from infant foreskins are grown in culture to produce thin sheets of cells that can be used to cover wounds, burns, or sores ...
... Cultured Skin Products • Skin is the only organ that is grown artificially for widespread medical uses – Cells from infant foreskins are grown in culture to produce thin sheets of cells that can be used to cover wounds, burns, or sores ...
Blimp1 regulates development of the posterior forelimb, caudal
... blood pooling within the dorsal aortae. The primitive heart seems to function normally, but placental development is severely compromised. Blimp1 mutants fail to expand the labyrinthine region where the bulk of foetal and maternal exchange takes place leading to placental insufficiency. In the embry ...
... blood pooling within the dorsal aortae. The primitive heart seems to function normally, but placental development is severely compromised. Blimp1 mutants fail to expand the labyrinthine region where the bulk of foetal and maternal exchange takes place leading to placental insufficiency. In the embry ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.