Expression of target protein and Affibody molecule in Pichia pastoris
... There are several reasons for choosing the Z domain as scaffold. It has proven very stable under alkaline conditions (Girot et al., 1990) as well as at high temperatures (Nord et al., 1995). It is small, highly soluble in aqueous solution (Samuelsson et al., 1994) and it contains no paired cysteines ...
... There are several reasons for choosing the Z domain as scaffold. It has proven very stable under alkaline conditions (Girot et al., 1990) as well as at high temperatures (Nord et al., 1995). It is small, highly soluble in aqueous solution (Samuelsson et al., 1994) and it contains no paired cysteines ...
Aging and Mitochondria - SENS Research Foundation
... •Arrange fibers properly in place •Secrete enzymes to digest old ECM Alberts, et.al. Molecular Biology of the Cell. (4th Ed. New York: Garland Publishing, Inc. 2002.) ...
... •Arrange fibers properly in place •Secrete enzymes to digest old ECM Alberts, et.al. Molecular Biology of the Cell. (4th Ed. New York: Garland Publishing, Inc. 2002.) ...
RNA binding proteins ZFP36L1 and ZFP36L2 promote cell quiescence
... DCKO late pre-B cells (Fig. 3A). Increased mRNA abundance in DCKO late pre-B cells was also associated with AREs in the 3’UTRs of mRNAs (fig. S8A), and with Zfp36 binding sites in the human homologs of mouse transcripts (15) (fig. S8B, C). Thus, the specificity of ZFP36 family proteins is generally ...
... DCKO late pre-B cells (Fig. 3A). Increased mRNA abundance in DCKO late pre-B cells was also associated with AREs in the 3’UTRs of mRNAs (fig. S8A), and with Zfp36 binding sites in the human homologs of mouse transcripts (15) (fig. S8B, C). Thus, the specificity of ZFP36 family proteins is generally ...
Identification of Diphtheria Toxin Receptor and a
... concentrations of octylglucoside, and the inhibitory activity recovered in the supernatant after centrifugation was determined. The inhibitory activity was partially released with octylglucoside at a concentration 15 mM, and maximal release was found at concentrations greater than 30 mM. The solubil ...
... concentrations of octylglucoside, and the inhibitory activity recovered in the supernatant after centrifugation was determined. The inhibitory activity was partially released with octylglucoside at a concentration 15 mM, and maximal release was found at concentrations greater than 30 mM. The solubil ...
Biology I Syllabus
... Identify and describe how subatomic particles are arranged in atoms? Describe the difference in ions and atoms and their importance in biological processes? Compare the types of bonding between atoms to form molecules? Explain the fundamental principles of the pH scale and the consequences o ...
... Identify and describe how subatomic particles are arranged in atoms? Describe the difference in ions and atoms and their importance in biological processes? Compare the types of bonding between atoms to form molecules? Explain the fundamental principles of the pH scale and the consequences o ...
Induction of apoptosis by the Amsacta moorei entomopoxvirus
... or mitochondrial (intrinsic) pathways, which result in the activation of effector caspases. The active effector caspases, through cleavage of specific host proteins, are instrumental in directing subsequent morphological and biochemical changes associated with apoptosis. These changes include reduct ...
... or mitochondrial (intrinsic) pathways, which result in the activation of effector caspases. The active effector caspases, through cleavage of specific host proteins, are instrumental in directing subsequent morphological and biochemical changes associated with apoptosis. These changes include reduct ...
potist power point
... Death by Amoeba: Some twin cities youth lost their lives to an amoeba which infected their brain after swimming in a certain lake ...
... Death by Amoeba: Some twin cities youth lost their lives to an amoeba which infected their brain after swimming in a certain lake ...
Syrosingopine sensitizes cancer cells to killing by metformin
... liver and muscle, results in reduced blood glucose levels and restoration of insulin responsiveness at the whole-body level. Metformin is a well-tolerated drug that can be taken for years with few adverse effects, such as rare cases of lactic acidosis. There is increasing evidence that metformin, in ...
... liver and muscle, results in reduced blood glucose levels and restoration of insulin responsiveness at the whole-body level. Metformin is a well-tolerated drug that can be taken for years with few adverse effects, such as rare cases of lactic acidosis. There is increasing evidence that metformin, in ...
Worksheet 1: Foundations—crossword
... Pot plant 1 is the control—it represents the ‘untreated’ plant that has been exposed to regular conditions. ...
... Pot plant 1 is the control—it represents the ‘untreated’ plant that has been exposed to regular conditions. ...
Fukuda, Annu. Rev. Plant Physiol. Plant Mol. Biol
... flow that starts initially by diffusion induces the formation of a polar auxin transport cell system, which in turn promotes auxin transport and leads to canalization of auxin flow along a narrow file of cells. This continuous polar transport of auxin through cells finally results in the differentia ...
... flow that starts initially by diffusion induces the formation of a polar auxin transport cell system, which in turn promotes auxin transport and leads to canalization of auxin flow along a narrow file of cells. This continuous polar transport of auxin through cells finally results in the differentia ...
The functions of cell wall polysaccharides in composition and
... Mutants with altered cellulose deposition have been identified directly by loss of birefringence, for example in trichomes (Potikha and Delmer, 1995). Other cell-wall mutations have appeared serendipitously in the course of screens for developmental phenotypes, such as a temperature-sensitive radial ...
... Mutants with altered cellulose deposition have been identified directly by loss of birefringence, for example in trichomes (Potikha and Delmer, 1995). Other cell-wall mutations have appeared serendipitously in the course of screens for developmental phenotypes, such as a temperature-sensitive radial ...
Glutamine breakdown in rapidly dividing cells: waste or investment?
... cells may require large amounts of glutamine for biosynthetic purposes, only a limited amount (<5%) of the extracted glutamine is used for such pathways.(8) However, it is noteworthy that, in rapidly dividing cells, the flux through glutaminase greatly exceeds the capacity of the mitochondria to oxi ...
... cells may require large amounts of glutamine for biosynthetic purposes, only a limited amount (<5%) of the extracted glutamine is used for such pathways.(8) However, it is noteworthy that, in rapidly dividing cells, the flux through glutaminase greatly exceeds the capacity of the mitochondria to oxi ...
Vesicle traffic in the endomembrane system: a tale of COPs, Rabs
... endosomes of mammalian cells. The membrane coats that are involved in vesicle formation are indicated next to the arrows where known. Dense vesicles (DV) are not formed by coats but by aggregation of cargo molecules. An alternative route into the PSV for certain storage proteins leads directly from ...
... endosomes of mammalian cells. The membrane coats that are involved in vesicle formation are indicated next to the arrows where known. Dense vesicles (DV) are not formed by coats but by aggregation of cargo molecules. An alternative route into the PSV for certain storage proteins leads directly from ...
Cells 1.2
... • The Golgi apparatus gathers simple molecules and combines them to make molecules that are more complex. • It then takes those big molecules, packages them in vesicles, and either stores them for later use or sends them out of the cell. • It is also the organelle that builds lysosomes (cell ...
... • The Golgi apparatus gathers simple molecules and combines them to make molecules that are more complex. • It then takes those big molecules, packages them in vesicles, and either stores them for later use or sends them out of the cell. • It is also the organelle that builds lysosomes (cell ...
Heart regeneration
... that a more recent study has called into question the effects of periostin on cell-cycle activity or cardiac repair21.) A recent study of the mitogenic effects of NRG1 showed that simple systemic injection of this growth factor into adult mice enhanced infarct scar shrinkage and improved mechanical ...
... that a more recent study has called into question the effects of periostin on cell-cycle activity or cardiac repair21.) A recent study of the mitogenic effects of NRG1 showed that simple systemic injection of this growth factor into adult mice enhanced infarct scar shrinkage and improved mechanical ...
Multiple Inducers of the Drosophila Heat Shock Locus 93D (hsro
... Figure I. The pattern of h,ro~ transcripts changes with time during heat shock. (A) Schematic diagram showing the genomic organization of the hsr~o locus (GENOMIC) and the structure of its transcripts (OMEGA 1, 2, and 3) in the Drosophila species studied. HSE/CTS indicates a region containing heat s ...
... Figure I. The pattern of h,ro~ transcripts changes with time during heat shock. (A) Schematic diagram showing the genomic organization of the hsr~o locus (GENOMIC) and the structure of its transcripts (OMEGA 1, 2, and 3) in the Drosophila species studied. HSE/CTS indicates a region containing heat s ...
The perichromatin region of the plant cell nucleus is the area with
... Fang et al. 2004), as speckles similar to those seen in animal cells. Plant speckles are morphologically diverse structures, and their shape and size depend on the species, cell type, and stage of development (Ali et al. 2003; Fang et al. 2004; Lorković et al. 2004). Treatment of plant cells with t ...
... Fang et al. 2004), as speckles similar to those seen in animal cells. Plant speckles are morphologically diverse structures, and their shape and size depend on the species, cell type, and stage of development (Ali et al. 2003; Fang et al. 2004; Lorković et al. 2004). Treatment of plant cells with t ...
RESPIRATION AND PROTEIN SYNTHESIS IN ESCHERICHIA COLI
... a N2 atmosphere. The reason for employing anaerobic conditions was so that we could study protein synthesis under conditions where it is not dependent on respiration . Tubes containing appropriate additions were flushed with N2 and stoppered . To start incubations 0 .3-1 .0 nil of the cell fraction ...
... a N2 atmosphere. The reason for employing anaerobic conditions was so that we could study protein synthesis under conditions where it is not dependent on respiration . Tubes containing appropriate additions were flushed with N2 and stoppered . To start incubations 0 .3-1 .0 nil of the cell fraction ...
Hemodynamic disorders p.1 - Patho
... 11 Kidney infarct 11a Early phase of the myocardial infarct 12 Lung infarct 10 Cancer cell embolism 290 Thrombus ...
... 11 Kidney infarct 11a Early phase of the myocardial infarct 12 Lung infarct 10 Cancer cell embolism 290 Thrombus ...
Higher Human Biology Exemplar Question Paper
... 10. Familial hypercholesterolaemia (FH) is caused by an autosomal dominant allele. The family history below shows the inheritance of FH through three generations. ...
... 10. Familial hypercholesterolaemia (FH) is caused by an autosomal dominant allele. The family history below shows the inheritance of FH through three generations. ...
Oncogenic ras-driven cancer cell vesiculation leads to emission of
... observed that addition of such EVs to cultures of RAT-1 cells leads to fluorescent labelling of the latter cells (Fig. 4A), a finding indicative of an avid uptake of EVs by these cells. To assess whether this interaction led to a transfer of H-ras DNA, recipient RAT-1 cells were lysed at different t ...
... observed that addition of such EVs to cultures of RAT-1 cells leads to fluorescent labelling of the latter cells (Fig. 4A), a finding indicative of an avid uptake of EVs by these cells. To assess whether this interaction led to a transfer of H-ras DNA, recipient RAT-1 cells were lysed at different t ...
Distribution, function, and prognostic value of cytotoxic T lymphocytes
... blood or from lymph node tissue were cocultured with either EBVtransformed B cells (JY)23 or with autologous FL B cells, respectively, which were either unpulsed or pulsed with bacterial superantigen cocktail (SAg, 100 ng/mL TSST-1, SEC 1, SEB, SEE). After 1 hour of culture, cells were stained with ...
... blood or from lymph node tissue were cocultured with either EBVtransformed B cells (JY)23 or with autologous FL B cells, respectively, which were either unpulsed or pulsed with bacterial superantigen cocktail (SAg, 100 ng/mL TSST-1, SEC 1, SEB, SEE). After 1 hour of culture, cells were stained with ...
Shininger, AnnRev. Plant Physiol. 1979 30:313-37
... differentiates with a variety of flexible arrangements(within and between organisms)into vascular and, in somecases, pith tissue. At a youngstage the cells withinthe central cyclinderdo not haveuniquecytologicalfeatures at the electron microscopelevel whichmightsuggest their early commitmentto diffe ...
... differentiates with a variety of flexible arrangements(within and between organisms)into vascular and, in somecases, pith tissue. At a youngstage the cells withinthe central cyclinderdo not haveuniquecytologicalfeatures at the electron microscopelevel whichmightsuggest their early commitmentto diffe ...
Second Harmonic Imaging of Plant Polysaccharides
... Cheng et al. 10 showed that plant chloroplasts are very susceptible to damage from femtosecond titanium sapphire laser irradiation. They used 760nm excitation, substantially shorter than the 830 or 840nm used here, and found that even a single 3.3 second scan or a small area, under their imaging con ...
... Cheng et al. 10 showed that plant chloroplasts are very susceptible to damage from femtosecond titanium sapphire laser irradiation. They used 760nm excitation, substantially shorter than the 830 or 840nm used here, and found that even a single 3.3 second scan or a small area, under their imaging con ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.