An A/ENTH Domain-Containing Protein Functions
... The Arabidopsis genome encodes 22 proteins with an A/ENTH domain. Phylogenetic analysis revealed that they can be grouped into five to six subfamilies (Supplemental Fig. S1; Holstein and Oliviusson, 2005). To gain insight into the role of these proteins in plant cells, the cellular localization of Ar ...
... The Arabidopsis genome encodes 22 proteins with an A/ENTH domain. Phylogenetic analysis revealed that they can be grouped into five to six subfamilies (Supplemental Fig. S1; Holstein and Oliviusson, 2005). To gain insight into the role of these proteins in plant cells, the cellular localization of Ar ...
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... PAP secondary antibody complex, most embryos were incubated with fluorescent secondary antibodies so that the fluorescent DNA-specific dye, Hoechst 33258, could be used to monitor nuclear and chromosomal morphology. Cells were classified as being in S phase on the basis of their nuclear incorporatio ...
... PAP secondary antibody complex, most embryos were incubated with fluorescent secondary antibodies so that the fluorescent DNA-specific dye, Hoechst 33258, could be used to monitor nuclear and chromosomal morphology. Cells were classified as being in S phase on the basis of their nuclear incorporatio ...
ASXL1 interacts with the cohesin complex to maintain chromatid
... chromatin disjunction Cohesin complex proteins embrace sister chromatids by forming a ring-like structure; the defective function of any of the core cohesin proteins disrupts the sister chromatid separation (32, 33). Myeloid cells with depleted Asxl1 exhibit a specific dysplastic feature as a pseudo ...
... chromatin disjunction Cohesin complex proteins embrace sister chromatids by forming a ring-like structure; the defective function of any of the core cohesin proteins disrupts the sister chromatid separation (32, 33). Myeloid cells with depleted Asxl1 exhibit a specific dysplastic feature as a pseudo ...
Respiratory
... The respiratory system structurally consists of paired lungs and the air conduction ways that connect to them. The latter includes the nasal cavity, pharynx, larynx, trachea and extra-pulmonary bronchi. As in all large organ systems, the respiratory system performs a number of functions: 1. Respirat ...
... The respiratory system structurally consists of paired lungs and the air conduction ways that connect to them. The latter includes the nasal cavity, pharynx, larynx, trachea and extra-pulmonary bronchi. As in all large organ systems, the respiratory system performs a number of functions: 1. Respirat ...
Certain Aspects of Cell Lineage and Morphogenesis
... mid-gut structure. By comparison with the controls (as shown in Text-fig. 2) it is obvious that irradiation at all three developmental stages has markedly reduced the number of pole cells which are eventually incorporated into the embryonic gonad. The results show quite definitely that it is those p ...
... mid-gut structure. By comparison with the controls (as shown in Text-fig. 2) it is obvious that irradiation at all three developmental stages has markedly reduced the number of pole cells which are eventually incorporated into the embryonic gonad. The results show quite definitely that it is those p ...
A Novel Plant Kinesin-Related Protein Specifically
... changed dynamically during the cell division cycle by using Arabidopsis root tip cells that were dividing actively (Figures 4 and 5). Dual localizations of AtPAKRP2 and tubulin were performed. During interphase, AtPAKRP2 was present throughout the cytoplasm and the nucleus, with a concentration of s ...
... changed dynamically during the cell division cycle by using Arabidopsis root tip cells that were dividing actively (Figures 4 and 5). Dual localizations of AtPAKRP2 and tubulin were performed. During interphase, AtPAKRP2 was present throughout the cytoplasm and the nucleus, with a concentration of s ...
Arabidopsis mutants in sphingolipid synthesis as tools to
... contiguous plant cells, originating interconnected symplastic domains. Communication arise through these intercellular pores that allow the exchange of small molecules, such as ions, sugars, phytohormones and macromolecules -RNA, transcription factors, even virus (Kim and Zambryski, 2005) and effect ...
... contiguous plant cells, originating interconnected symplastic domains. Communication arise through these intercellular pores that allow the exchange of small molecules, such as ions, sugars, phytohormones and macromolecules -RNA, transcription factors, even virus (Kim and Zambryski, 2005) and effect ...
The Dictyostelium LvsA Protein is Localized on the Contractile
... beige-like protein lba was shown to localize to vesicles in response to lipopolysaccharide stimulation (11). Biochemical studies have suggested that other beige proteins are cytosolic (2,4). This raises the interesting possibility that beige-related proteins associate with specific membrane compartm ...
... beige-like protein lba was shown to localize to vesicles in response to lipopolysaccharide stimulation (11). Biochemical studies have suggested that other beige proteins are cytosolic (2,4). This raises the interesting possibility that beige-related proteins associate with specific membrane compartm ...
The Expression of MHC Class II Genes in Macrophages Is Cell
... could also affect the translational apparatus and regulate in a CCD way the translation of several genes (7, 8). Therefore, there are genes induced in a CCD fashion that are not involved in cell cycle progression. This is the case of genes that control some cellular activities. For example, it has b ...
... could also affect the translational apparatus and regulate in a CCD way the translation of several genes (7, 8). Therefore, there are genes induced in a CCD fashion that are not involved in cell cycle progression. This is the case of genes that control some cellular activities. For example, it has b ...
The synthesis and migration of nuclear proteins during mitosis and
... the experimental method, the type of nucleus, or the amino-acid that they used. Evidence of a different type which shows that protein leaves the nucleus during mitosis is the loss of mass suffered by the nucleus during prophase. This has been demonstrated in endosperms by Richards and Bajer (1961) a ...
... the experimental method, the type of nucleus, or the amino-acid that they used. Evidence of a different type which shows that protein leaves the nucleus during mitosis is the loss of mass suffered by the nucleus during prophase. This has been demonstrated in endosperms by Richards and Bajer (1961) a ...
Effect of Butyric Acid on Lung-colonizing Ability
... noma cells (P-29) was enhanced by their in vitro treatment with butyric acid and its sodium salt, sodium butyrate. Of the short chain fatty acids tested, butyric acid was the most effective in enhancing the lung-colonizing ability of P-29 cells; propionic acid and valeric acid were slightly effectiv ...
... noma cells (P-29) was enhanced by their in vitro treatment with butyric acid and its sodium salt, sodium butyrate. Of the short chain fatty acids tested, butyric acid was the most effective in enhancing the lung-colonizing ability of P-29 cells; propionic acid and valeric acid were slightly effectiv ...
PPT - Altogen Biosystems
... Products > SK-MEL-28 Transfection Reagent (Melanoma Cells, HTB72) Altogen Biosystems offers the SK-MEL-28 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The SK-MEL-28 Transfection Reagent is a nanoparticle-based liposome formulation, and it has been develope ...
... Products > SK-MEL-28 Transfection Reagent (Melanoma Cells, HTB72) Altogen Biosystems offers the SK-MEL-28 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The SK-MEL-28 Transfection Reagent is a nanoparticle-based liposome formulation, and it has been develope ...
Cell Wall Loosening by Expansins
... but not in other tissues or species (pollen allergens from ragweed and other species outside the grass family are not homologous with the group-1 allergens); (b) vegetative homologs of the group-1 allergens expressed in grass seedlings (not in pollen), at least seven of which are expressed in young ...
... but not in other tissues or species (pollen allergens from ragweed and other species outside the grass family are not homologous with the group-1 allergens); (b) vegetative homologs of the group-1 allergens expressed in grass seedlings (not in pollen), at least seven of which are expressed in young ...
Why do muscles shorten? 112ch11
... Cardiac cells depolarize for long periods and do not have the quick twitches that are characteristic of skeletal muscle Cardiac cells are linked by Gap Junctions so when Na+ enters a cell it can cross into and depolarize the next cell and so-on. While each skeletal myofiber requires a depolarization ...
... Cardiac cells depolarize for long periods and do not have the quick twitches that are characteristic of skeletal muscle Cardiac cells are linked by Gap Junctions so when Na+ enters a cell it can cross into and depolarize the next cell and so-on. While each skeletal myofiber requires a depolarization ...
Expression and Endocytosis of Lysosomal
... from telencephalon of 14- to 16-d-old AGU mouse (Jalanko et al., 1998) embryos. Meningeal membranes were removed in an ice-cold solution of PBS –20 mM glucose, and the brain tissue was gently triturated with a 5 ml pipette in PBS –20 mM glucose containing trypsin-EDTA (0.1/0.04%) and DNase I (10 mg ...
... from telencephalon of 14- to 16-d-old AGU mouse (Jalanko et al., 1998) embryos. Meningeal membranes were removed in an ice-cold solution of PBS –20 mM glucose, and the brain tissue was gently triturated with a 5 ml pipette in PBS –20 mM glucose containing trypsin-EDTA (0.1/0.04%) and DNase I (10 mg ...
Processing of human cytomegalovirus envelope glycoproteins in
... 1.0~ NP40. Cells were extracted for 1 h with constant mixing. Insoluble material was removed by centrifugation and the supernatant used for immunoprecipitations. Immunoprecipitations were done using biotinylated MAbs and streptavidin-agarose as described (Gretch et aL, 1987). Plaque assay. Cultures ...
... 1.0~ NP40. Cells were extracted for 1 h with constant mixing. Insoluble material was removed by centrifugation and the supernatant used for immunoprecipitations. Immunoprecipitations were done using biotinylated MAbs and streptavidin-agarose as described (Gretch et aL, 1987). Plaque assay. Cultures ...
Yersinia pestis targets neutrophils via complement receptor 3
... Because there was a broad range of bacterial load in infected mice, particularly on day 3 post-infection, we chose to pool the data from all days and then categorize according to the bacterial loads using 1-log increments (Fig. 1C). In this way, the ability of the ail mutant to target immune cells c ...
... Because there was a broad range of bacterial load in infected mice, particularly on day 3 post-infection, we chose to pool the data from all days and then categorize according to the bacterial loads using 1-log increments (Fig. 1C). In this way, the ability of the ail mutant to target immune cells c ...
Ultrastructural studies of the mouse blastocyst
... blastocyst staging and description are apparent in the light of several recent investigations. For example, Stern (1972) and Stern & Wilson (1972) found that mouse blastocyst cells are developmentally labile, and Gardner (1972) reported that the continued presence of the inner cell mass is necessary ...
... blastocyst staging and description are apparent in the light of several recent investigations. For example, Stern (1972) and Stern & Wilson (1972) found that mouse blastocyst cells are developmentally labile, and Gardner (1972) reported that the continued presence of the inner cell mass is necessary ...
Defined, consistent quality SYNTHETIC BIOLOGY
... GeneArt® Algae Engineering Kits are designed and manufactured to help ensure that the starting materials are always consistent and the results are reproducible. • Algal cells arrive ready to resuscitate, grow, and transform or store at –80°C until ready to use • Every cell lot is manufactured using ...
... GeneArt® Algae Engineering Kits are designed and manufactured to help ensure that the starting materials are always consistent and the results are reproducible. • Algal cells arrive ready to resuscitate, grow, and transform or store at –80°C until ready to use • Every cell lot is manufactured using ...
Life Science Semester 1 Final Exam Review Sheet
... Prokaryotic and eukaryotic cells Parts and functions of cells Compare and contrast chart: animal, plant cells Anatomy of a cell Cell treasure hunt Structures of Life video clip Summarize organelle functions: 3 words per organelle Cell analogy Edible Cell Lab A visit to Cellville Organelle flashcards ...
... Prokaryotic and eukaryotic cells Parts and functions of cells Compare and contrast chart: animal, plant cells Anatomy of a cell Cell treasure hunt Structures of Life video clip Summarize organelle functions: 3 words per organelle Cell analogy Edible Cell Lab A visit to Cellville Organelle flashcards ...
Direct Visualization by Cryo-EM of the Mycobacterial Capsular
... antisera against known capsular components was performed. Whole bacteria were immobilized on EM grids and incubated with specific antibodies and gold probes. This method also allowed us to visualize and quantify the different components. Since a-glucan is described to be the major capsular polysacch ...
... antisera against known capsular components was performed. Whole bacteria were immobilized on EM grids and incubated with specific antibodies and gold probes. This method also allowed us to visualize and quantify the different components. Since a-glucan is described to be the major capsular polysacch ...
The Par3/Par6/aPKC Complex and Epithelial Cell Polarity
... In one-cell embryos such as C.elegans embryos where the anterior-posterior polarity is believed to be generated stochastically by actomyosin flow at the sperm entry site [2]. In multicellular system, “touching” by neighbors is a resemblance of fertilization process and is crucial for the later diffe ...
... In one-cell embryos such as C.elegans embryos where the anterior-posterior polarity is believed to be generated stochastically by actomyosin flow at the sperm entry site [2]. In multicellular system, “touching” by neighbors is a resemblance of fertilization process and is crucial for the later diffe ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.