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Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > SK-MEL-28 Transfection Reagent (Melanoma Cells, HTB72)
Altogen Biosystems offers the SK-MEL-28 Transfection
Reagent among a host of 100+ cell line specific In Vitro
Transfection Kits.
The SK-MEL-28 Transfection Reagent is a nanoparticle-based
liposome formulation, and it has been developed to provide high
transfection efficiency with the SK-MEL-28 cell line.
This cell line is a good host for studying skin cancer. When
cultured in vitro, SK-MEL-28 cells are a monolayer and adhere
to the surface of the culture flask.
Other applications include drug discovery, gene expression
studies, molecular and cell biology research applications.
Purchase SK-MEL-28 Transfection Kit at www.Altogen.com
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > SK-MEL-28 Transfection Reagent (Melanoma Cells, HTB72)
SK-MEL-28 Cell Line Description and Applications
SK-MEL-28 cells
ezbiosystems.com
The SK-MEL-28 cell line originates from the skin
tissue of a 51-year old male patient suffering from
malignant melanoma skin cancer. The cell line
displays polygonal cell morphology. The SK-MEL-28
cell line has a modal number of 90 and is a
hypotetraploid. The name “SK-MEL” is an umbrella
for several melanoma cell lines, and SK-MEL-28 is
one particular strain. All of the SK-MEL lines,
including SK-MEL-28 are useful in research related to
cell and molecular biology, but the SK-MEL-28 cell
line is also useful for studying skin cancer in
particular.
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > SK-MEL-28 Transfection Reagent (Melanoma Cells, HTB72)
SK-MEL-28 Transfection Protocol
1. Plate 10,000 - 15,000 SK-MEL-28 cells per well in 0.5 ml of complete growth medium 12–24 hours prior to
transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent,
and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for 15 - 30 minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex,
therefore increasing transfection efficiency; however it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with SK-MEL-28 cells (from
step 2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression 48 - 72 hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of
Transfection Enhancer reagent 12-24 hours after transfection
If the viability of SK-MEL-28 cells being transfected is affected at 16 - 24 hours post-transfection, the level of
cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to
transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > SK-MEL-28 Transfection Reagent (Melanoma Cells, HTB72)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > SK-MEL-28 Transfection Reagent (Melanoma Cells, HTB72)
SK-MEL-28 Transfection Kit Product Details
•
A nanoparticle-based liposome formulation
•
Optimized for intracellular delivery of plasmid DNA, siRNA, microRNA, and mRNA
•
High transfection efficiency of both siRNA and plasmid DNA without compromising cell viability
•
Achieve robust siRNA uptake for dependable gene silencing
•
Effective transfection under conditions of up to 40% serum
•
Transfection kit includes Transfection Enhancer reagent
•
Gentle enough to be used for single cell analysis
Developed and manufactured by Altogen Biosystems (www.Altogen.com)
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > SK-MEL-28 Transfection Reagent (Melanoma Cells, HTB72)
Data
Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into SKMEL-28 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed
by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin
A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > SK-MEL-28 Transfection Reagent (Melanoma Cells, HTB72)
Data
Figure 2. Protein expression of Lamin A/C in SK-MEL-28 cells. DNA plasmid expressing Lamin A/C or siRNA
targeting Lamin A/C were transfected into SK-MEL-28 cells following Altogen Biosystems transfection protocol.
At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized
by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > SK-MEL-28 Transfection Reagent (Melanoma Cells, HTB72)
SK-MEL-28 Transfection Kit Benefits
• Pre-optimized transfection protocol for SK-MEL-28 cell line
• Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
• Free of serum and protein of animal origin
• Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
• Easy to use SK-MEL-28 transfection protocol ensures great performance with expedited experimental
timeline
• Equally efficient for single or multiple transfections
• Can be used for transient transfection and development of stable SK-MEL-28 cell lines
• Bio-degradable after endocytosis
• Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]