Functional differences between kindlin-1 and kindlin
... directly bind to the membrane-distal NPxY motifs within the b integrin tail. Only two of the three mammalian kindlins are expressed in keratinocytes: the epithelial-specific kindlin-1 and the essential ubiquitously expressed kindlin-2 (Lai-Cheong et al., 2008). Mutations in the kindlin-1 gene cause ...
... directly bind to the membrane-distal NPxY motifs within the b integrin tail. Only two of the three mammalian kindlins are expressed in keratinocytes: the epithelial-specific kindlin-1 and the essential ubiquitously expressed kindlin-2 (Lai-Cheong et al., 2008). Mutations in the kindlin-1 gene cause ...
Title of the Project Cellular and molecular mechanisms of ozone
... be involved in the action mechanism of ozone therapy since they can induce epigenetic changes in gene or protein expression, which persist over time and play important regulatory roles in a variety of biological processes (8). The use of in vitro systems will ensure controlled and easily reproducibl ...
... be involved in the action mechanism of ozone therapy since they can induce epigenetic changes in gene or protein expression, which persist over time and play important regulatory roles in a variety of biological processes (8). The use of in vitro systems will ensure controlled and easily reproducibl ...
BMP Induction of Id Proteins Suppresses Differentiation
... The self-renewal action of LIF is mediated via the transcription factor STAT3 (Matsuda et al., 1999; Niwa et al., 1998). BMP alone does not activate STAT3 measured by phosphorylation of tyrosine 705 (Figure 3C), nor does it increase STAT3 activation by LIF. Erk activation downstream of gp130 is not ...
... The self-renewal action of LIF is mediated via the transcription factor STAT3 (Matsuda et al., 1999; Niwa et al., 1998). BMP alone does not activate STAT3 measured by phosphorylation of tyrosine 705 (Figure 3C), nor does it increase STAT3 activation by LIF. Erk activation downstream of gp130 is not ...
PPT - Altogen Biosystems
... Products > Transfection Reagent for C6 Cells (Glioma Cells, CCL-107) Altogen Biosystems offers the C6 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The C6 Transfection Reagent is an advanced lipid formulation reagent, and it has been developed to provide hi ...
... Products > Transfection Reagent for C6 Cells (Glioma Cells, CCL-107) Altogen Biosystems offers the C6 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The C6 Transfection Reagent is an advanced lipid formulation reagent, and it has been developed to provide hi ...
November 2013 Life Science Strand
... brief explanation of how the document is organized. As you use it, it will become clearer. First, the document is organized by topics. These topics include: Cell Biology, Genetics, Anatomy and Physiology, Ecology, and Evolution & Biodiversity. Each topic presents a standard. Standards are what learn ...
... brief explanation of how the document is organized. As you use it, it will become clearer. First, the document is organized by topics. These topics include: Cell Biology, Genetics, Anatomy and Physiology, Ecology, and Evolution & Biodiversity. Each topic presents a standard. Standards are what learn ...
the effect of 5-bromodeoxyuridine on dna replication and
... from the medium at the time of addition of thymidine . To follow the DNA replication in the population after the addition of BUdR and again after the removal of BUdR, the DNA was uniformly labeled after several generations of growth in the presence of [ 3 H]thymidine . These labeled cells were used ...
... from the medium at the time of addition of thymidine . To follow the DNA replication in the population after the addition of BUdR and again after the removal of BUdR, the DNA was uniformly labeled after several generations of growth in the presence of [ 3 H]thymidine . These labeled cells were used ...
Journal of Microbiological Methods
... this might be the high variability of the nitrite reductase even in strains of the same species. This made an overall detection of active denitrifiers impossible by immunological tools. Using a newly described monoclonal antibody (mAb) (Metz et al., 2002), it was possible to overcome this problem at ...
... this might be the high variability of the nitrite reductase even in strains of the same species. This made an overall detection of active denitrifiers impossible by immunological tools. Using a newly described monoclonal antibody (mAb) (Metz et al., 2002), it was possible to overcome this problem at ...
Simple microwave field imaging technique using hot atomic vapor
... to buffer gas is known to decrease optical density,22 but the decrease we observe is much higher than expected for pure Ne. We are confident that these issues can be resolved using a different vapor cell fabrication technique. As reported in ref.15 , microfabricated alkali vapor cells with Ne buffer ...
... to buffer gas is known to decrease optical density,22 but the decrease we observe is much higher than expected for pure Ne. We are confident that these issues can be resolved using a different vapor cell fabrication technique. As reported in ref.15 , microfabricated alkali vapor cells with Ne buffer ...
That bacteria may be able to mutate in response to
... Using the techniques of Chicago colleague Malcolm Casadaban, Shapiro worked with an E. coli strain which was engineered to study hybrid proteins. Shapiro's construct had an arabinose regulatory gene followed by an arabinose structural gene into which was inserted a Mu prophage. Further downstream wa ...
... Using the techniques of Chicago colleague Malcolm Casadaban, Shapiro worked with an E. coli strain which was engineered to study hybrid proteins. Shapiro's construct had an arabinose regulatory gene followed by an arabinose structural gene into which was inserted a Mu prophage. Further downstream wa ...
hormone
... • The word hormone is derived from a Greek verb meaning “to excite.” • Found in all multicellular organisms, hormones are chemical signals that are produced in one part of the body, transported to other parts, bind to specific receptors, and trigger responses in targets cells and tissues. • Only min ...
... • The word hormone is derived from a Greek verb meaning “to excite.” • Found in all multicellular organisms, hormones are chemical signals that are produced in one part of the body, transported to other parts, bind to specific receptors, and trigger responses in targets cells and tissues. • Only min ...
Spatial regularity among retinal neurons
... somata are often called ‘neuronal mosaics’ even if precise dendritic tiling has not been shown directly, and the term ‘mosaic’ is used in this sense throughout the chapter. It is important to be aware that geneticists and developmental biologists exploit the analogy of the tiled mosaic in another wa ...
... somata are often called ‘neuronal mosaics’ even if precise dendritic tiling has not been shown directly, and the term ‘mosaic’ is used in this sense throughout the chapter. It is important to be aware that geneticists and developmental biologists exploit the analogy of the tiled mosaic in another wa ...
Differential roles for the low-affinity phosphate transporters Pho87
... for expression of the so-called STRE (stress-responsive element) genes and the PDS (post-diauxic shift) genes respectively [12–14]. Rim15 acts as a nutritional integrator in yeast, since it is regulated by at least four nutrient-regulated kinases: PKA (protein kinase A) inhibits Rim15 activity [15], ...
... for expression of the so-called STRE (stress-responsive element) genes and the PDS (post-diauxic shift) genes respectively [12–14]. Rim15 acts as a nutritional integrator in yeast, since it is regulated by at least four nutrient-regulated kinases: PKA (protein kinase A) inhibits Rim15 activity [15], ...
Aggresomes: A Cellular Response to Misfolded Proteins
... Given the potentially pathogenic consequences of intracellular protein aggregation, it is not surprising that eukaryotic cells have evolved mechanisms to prevent their formation or accumulation. Molecular chaperones bind nonnative protein conformations, sequestering misfolded protein or aggregation- ...
... Given the potentially pathogenic consequences of intracellular protein aggregation, it is not surprising that eukaryotic cells have evolved mechanisms to prevent their formation or accumulation. Molecular chaperones bind nonnative protein conformations, sequestering misfolded protein or aggregation- ...
COMMENTARY REDOX SIGNALLING BY TRANSCRIPTION
... of the catalase and SOD gene [17]. In plants, salicylic acid (SA) is known as a mediator for systemic acquired resistance, an equivalent of the vertebrate immune response. Recently, the SA-binding protein has been shown to contain catalase activity, which decreases significantly upon ligand binding ...
... of the catalase and SOD gene [17]. In plants, salicylic acid (SA) is known as a mediator for systemic acquired resistance, an equivalent of the vertebrate immune response. Recently, the SA-binding protein has been shown to contain catalase activity, which decreases significantly upon ligand binding ...
Asymmetries in Cell Division, Cell Size, and Furrowing in the
... Xenopus laevis—a model used in developmental biology research for more than a century—allowed discovering basic principles of early development in vertebrates including asymmetric distribution of cell fate determinants, which leads to dorsalization of the embryo. Although the asymmetric cell-size di ...
... Xenopus laevis—a model used in developmental biology research for more than a century—allowed discovering basic principles of early development in vertebrates including asymmetric distribution of cell fate determinants, which leads to dorsalization of the embryo. Although the asymmetric cell-size di ...
Centrosome Biology: A SAS-sy Centriole in the Cell Cycle Dispatch
... pericentriolar material that nucleate and anchor microtubules. It should be noted that eukaryotes present a continuum of microtubule organization strategies. In yeast and other fungi, all microtubules grow from highly ordered centrosome-like organelles. In contrast, higher plants lack anything resem ...
... pericentriolar material that nucleate and anchor microtubules. It should be noted that eukaryotes present a continuum of microtubule organization strategies. In yeast and other fungi, all microtubules grow from highly ordered centrosome-like organelles. In contrast, higher plants lack anything resem ...
Overexpression of vinculin suppresses cell motility in BALB/c 3T3 cells
... overexpression of vinculin in 3T3 cells by about 20%, following transfection, brings about a major change in the dynamic properties of cells. This was manifested by an extensive decrease in the migration of the transfected cells into an artificial wound and in a significant reduction in locomotory a ...
... overexpression of vinculin in 3T3 cells by about 20%, following transfection, brings about a major change in the dynamic properties of cells. This was manifested by an extensive decrease in the migration of the transfected cells into an artificial wound and in a significant reduction in locomotory a ...
PDF
... In situ hybridizations were carried out according to a previously established protocol (Essner et al., 2000) using a Biolane HTI in situ machine (Huller and Huttner AG, Tübingen, Germany). Embryos were cleared in 70% glycerol in PBST and photographed with a Nikon SMZ1000 on a Leica MZ12 dissecting m ...
... In situ hybridizations were carried out according to a previously established protocol (Essner et al., 2000) using a Biolane HTI in situ machine (Huller and Huttner AG, Tübingen, Germany). Embryos were cleared in 70% glycerol in PBST and photographed with a Nikon SMZ1000 on a Leica MZ12 dissecting m ...
Embryonic stem cells form an organized, functional
... undifferentiated ES cells containing both vector constructs (minKlacZ and cGATA6-ERFP) were generated, they were differentiated using the “hanging-drop” method as previously described (32). Briefly, 20-l drops containing 200 ES cells each in differentiation medium (growth medium without leukemia in ...
... undifferentiated ES cells containing both vector constructs (minKlacZ and cGATA6-ERFP) were generated, they were differentiated using the “hanging-drop” method as previously described (32). Briefly, 20-l drops containing 200 ES cells each in differentiation medium (growth medium without leukemia in ...
Overexpression of a truncated cyclin B gene arrests Dictyostelium
... used to transform exponential phase Ax-2 cells by the CaPO4 DNA precipitation method (Nellen et al., 1984) in bis-Tris HL-5 (Egelhoff et al., 1989). Stable transformants were selected and grown clonally in HL-5 medium containing 10 µg/ml of G418, 50 µg/ml of streptomycin, 50 µg/ml of ampicillin and ...
... used to transform exponential phase Ax-2 cells by the CaPO4 DNA precipitation method (Nellen et al., 1984) in bis-Tris HL-5 (Egelhoff et al., 1989). Stable transformants were selected and grown clonally in HL-5 medium containing 10 µg/ml of G418, 50 µg/ml of streptomycin, 50 µg/ml of ampicillin and ...
Topic B1
... Activity 1 from the Cell Level Systems Delivery Guide here can be used to see what learners remember about DNA structure from previous learning. Introduce more about the structure of DNA by describing the role of modelling used by Watson and Crick in their determination of the structure. Show video ...
... Activity 1 from the Cell Level Systems Delivery Guide here can be used to see what learners remember about DNA structure from previous learning. Introduce more about the structure of DNA by describing the role of modelling used by Watson and Crick in their determination of the structure. Show video ...
S-layer and cytoplasmic membrane – exceptions from the typical
... With a second, outermost membrane, you get at least two separated compartments like in Gram-negative bacteria: the cytoplasm and the (pseudo)periplasm (Rigel and Silhavy, 2012). In Gramnegative bacteria, the periplasm can make up about 10% of the cell volume and constitutes an oxidizing environment, ...
... With a second, outermost membrane, you get at least two separated compartments like in Gram-negative bacteria: the cytoplasm and the (pseudo)periplasm (Rigel and Silhavy, 2012). In Gramnegative bacteria, the periplasm can make up about 10% of the cell volume and constitutes an oxidizing environment, ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.