Detection of Apoptosis in Paraffin Embedded Tissues: the Influence
... With increasing importance of apoptosis, many assays have been developed to detect apoptosis in cell populations and individual cells. Apoptotic cells are often proved using TUNEL assay based on detection of DNA strand breaks that occur during apoptosis. The method enables quantification of apoptoti ...
... With increasing importance of apoptosis, many assays have been developed to detect apoptosis in cell populations and individual cells. Apoptotic cells are often proved using TUNEL assay based on detection of DNA strand breaks that occur during apoptosis. The method enables quantification of apoptoti ...
Network structure determines patterns of network
... They develop synaptic inputs from GABAergic interneurons whose activity promotes the development of neural processes (9; 10). After weeks, they form glutamergic synapses onto mossy cells and interneurons (11). Studies have found new granule cells to be preferentially activated by stimulation (12) an ...
... They develop synaptic inputs from GABAergic interneurons whose activity promotes the development of neural processes (9; 10). After weeks, they form glutamergic synapses onto mossy cells and interneurons (11). Studies have found new granule cells to be preferentially activated by stimulation (12) an ...
Lecture 19. secondary phloem-2
... • Companion Cells are derived from the same cell which forms a Sieve Tube Member. They control the physiological processes involved in phloem transport. • Albuminous Cells perform a similar function for Sieve Cells. However, they are not a direct descendent of the cell which produces the Sieve Cell. ...
... • Companion Cells are derived from the same cell which forms a Sieve Tube Member. They control the physiological processes involved in phloem transport. • Albuminous Cells perform a similar function for Sieve Cells. However, they are not a direct descendent of the cell which produces the Sieve Cell. ...
RNA nuclear export is blocked by poliovirus 2A protease and is
... death (p53) and in cell proliferation (c-myc) was analyzed in these cells. As expected, the most abundant mRNA in the total fraction of control cells was -actin, whereas IL-2 mRNA was not detected (supplementary material Fig. S1E). Steady-state levels of IL-6, cmyc and p53 mRNAs increased in cells ...
... death (p53) and in cell proliferation (c-myc) was analyzed in these cells. As expected, the most abundant mRNA in the total fraction of control cells was -actin, whereas IL-2 mRNA was not detected (supplementary material Fig. S1E). Steady-state levels of IL-6, cmyc and p53 mRNAs increased in cells ...
Human Body Review
... 37. The human body has different types of muscle tissue made of protein. The way the protein is arranged determines what the muscle does. What does this most likely mean about the structure of the muscles? A. ...
... 37. The human body has different types of muscle tissue made of protein. The way the protein is arranged determines what the muscle does. What does this most likely mean about the structure of the muscles? A. ...
Heading forwards: anterior visceral endoderm migration in
... Basal projections driven by Rho-GTPases and the WAVE complex activity are sent out in the direction of migration forming new cell–cell contact sites (blue gradients). Progressively, apical junctional complexes are turned over and remodelled at the leading edge and back of the cell as the cortical ac ...
... Basal projections driven by Rho-GTPases and the WAVE complex activity are sent out in the direction of migration forming new cell–cell contact sites (blue gradients). Progressively, apical junctional complexes are turned over and remodelled at the leading edge and back of the cell as the cortical ac ...
Small airway-on-a-chip enables analysis of human lung
... adhesion to ICAM-1 (ref: 16) and this dynamic shear stress-dependent response cannot be studied in a physiologically relevant way using static cell cultures. Advances in microsystems engineering have recently made it possible to create biomimetic microfluidic cell culture devices, known as ‘organs-o ...
... adhesion to ICAM-1 (ref: 16) and this dynamic shear stress-dependent response cannot be studied in a physiologically relevant way using static cell cultures. Advances in microsystems engineering have recently made it possible to create biomimetic microfluidic cell culture devices, known as ‘organs-o ...
The constant beat: cardiomyocytes adapt their forces by equal
... mammalian cells. The consequences extend from simple, small adaptations of rather isolated cellular structures all the way to initiation of very complex differentiation pathways. One of the most potent mechanical signals is the elasticity of the environment. Various cell types respond with simple re ...
... mammalian cells. The consequences extend from simple, small adaptations of rather isolated cellular structures all the way to initiation of very complex differentiation pathways. One of the most potent mechanical signals is the elasticity of the environment. Various cell types respond with simple re ...
An ARL1 mutation affected autophagic cell death in yeast
... themselves in 7 days by activating autophagic machinery. In parallel, the cell-proliferative activity decreases progressively after about 48 h. We have previously referred to this phenomenon as autophagic death. In the present study, we isolated and characterized a recessive mutant strain, dlp2, whi ...
... themselves in 7 days by activating autophagic machinery. In parallel, the cell-proliferative activity decreases progressively after about 48 h. We have previously referred to this phenomenon as autophagic death. In the present study, we isolated and characterized a recessive mutant strain, dlp2, whi ...
Biofilm-grown Mycoplasma mycoides subsp. mycoides SC
... flatter biofilm which was still several cells deep, and with microcolonies around the edge of the ...
... flatter biofilm which was still several cells deep, and with microcolonies around the edge of the ...
Mutations in a novel gene, myoblast city, provide evidence
... cells are only slightly elongated, but from about 15 hours AEL In embryos mutant for mbc, myosin expression begins on they stretch and send out long processes (Fig. 1C). These two schedule at 9 hours AEL, in cells that appear by their position myoblast populations and their behaviours are reminiscen ...
... cells are only slightly elongated, but from about 15 hours AEL In embryos mutant for mbc, myosin expression begins on they stretch and send out long processes (Fig. 1C). These two schedule at 9 hours AEL, in cells that appear by their position myoblast populations and their behaviours are reminiscen ...
Targeted disruption of fibroblast growth factor receptor
... showed very similar tendency. Only high-affinity binding of 125I-FGF-2 in wildtype ES cells was statistically significant. This binding was not observed in the corresponding mutant cells. Standard deviations are indicated. (*, p < 0.05 versus binding in the presence of unlabeled FGF-2). Fig. 3. (Rig ...
... showed very similar tendency. Only high-affinity binding of 125I-FGF-2 in wildtype ES cells was statistically significant. This binding was not observed in the corresponding mutant cells. Standard deviations are indicated. (*, p < 0.05 versus binding in the presence of unlabeled FGF-2). Fig. 3. (Rig ...
Multiple Mechanisms of Resistance to Polyglutamatable and
... included 100 mM Pyr. Before experiments, PyrR100 cells were transferred at least three passages without Pyr. In other experiments, AA8 and PyrR100 cells were maintained in folate-deplete RPMI medium consisting of folate-free RPMI-1640 medium, 10% dialyzed FCS, 2 mM L-glutamine, and 100 units/ml of b ...
... included 100 mM Pyr. Before experiments, PyrR100 cells were transferred at least three passages without Pyr. In other experiments, AA8 and PyrR100 cells were maintained in folate-deplete RPMI medium consisting of folate-free RPMI-1640 medium, 10% dialyzed FCS, 2 mM L-glutamine, and 100 units/ml of b ...
Induction of Mac-2BP by nerve growth factor is
... regulate cell adhesion by binding to cellular matrix proteins including β1-integrin, collagens, and fibronectins (13). Therefore, Mac-2BP may favor the establishment of new tumor colonies via enhancement of adhesive interactions between tumor cells and the extracellular matrix (14,15). Neurotrophins ...
... regulate cell adhesion by binding to cellular matrix proteins including β1-integrin, collagens, and fibronectins (13). Therefore, Mac-2BP may favor the establishment of new tumor colonies via enhancement of adhesive interactions between tumor cells and the extracellular matrix (14,15). Neurotrophins ...
regulator of cell-plate formation in plant cytokinesis The NPK1
... The antibodies against NPK1 that had been preincubated with the above-mentioned oligopeptide did not stain this region (Fig. 2B, telophase). During expansion of the phragmoplast from early to late telophase, the signal caused by NPK1 was consistently detected in the MTinterdigitating region of the r ...
... The antibodies against NPK1 that had been preincubated with the above-mentioned oligopeptide did not stain this region (Fig. 2B, telophase). During expansion of the phragmoplast from early to late telophase, the signal caused by NPK1 was consistently detected in the MTinterdigitating region of the r ...
Early transcription in Caenorhabditis elegans embryos
... mRNAs, an estimated 20 to 30 of which are expressed at high levels only during this period (Schauer and Wood, 1990). However, these experiments provided only an approximation of the time of transcription onset. We have carried out in vivo assays of early transcription to define more precisely when i ...
... mRNAs, an estimated 20 to 30 of which are expressed at high levels only during this period (Schauer and Wood, 1990). However, these experiments provided only an approximation of the time of transcription onset. We have carried out in vivo assays of early transcription to define more precisely when i ...
Ameba Coloring
... There are two types of nuclei (plural of nucleus). The large nucleus is called the macronucleus which controls cell activities such as respiration, protein synthesis and digestion. Color the macronucleus red. The much smaller micronucleus is used only during reproduction, color the micronucleus pink ...
... There are two types of nuclei (plural of nucleus). The large nucleus is called the macronucleus which controls cell activities such as respiration, protein synthesis and digestion. Color the macronucleus red. The much smaller micronucleus is used only during reproduction, color the micronucleus pink ...
1. Characteristics of living organisms Core • List and describe the
... • List the principal sources of, and describe the • carbohydrates • proteins • mineral salts (calcium and iron only) • water • vitamins (C and D only) • Describe the use of microorganisms in the single cell protein hazards associated with food additives, 6.2 Plant nutrition Core process by which pla ...
... • List the principal sources of, and describe the • carbohydrates • proteins • mineral salts (calcium and iron only) • water • vitamins (C and D only) • Describe the use of microorganisms in the single cell protein hazards associated with food additives, 6.2 Plant nutrition Core process by which pla ...
File
... In multicellular organisms the eukaryotic cells are specialised to perform different functions. Thousands of different types of cell have been described, such as red blood cells, smooth muscle cells, adipose cells, B lymphocytes, osteocytes, motor neurones, ova, ciliated epithelial cells, endocrine ...
... In multicellular organisms the eukaryotic cells are specialised to perform different functions. Thousands of different types of cell have been described, such as red blood cells, smooth muscle cells, adipose cells, B lymphocytes, osteocytes, motor neurones, ova, ciliated epithelial cells, endocrine ...
A Dictyostelium Mutant with Reduced Lysozyme Levels
... types, it accumulates non-degradable material and retains it for the lifetime of the cell and organism. In amoebae, endocytosed cargo transits through the cell. Any material that cannot be degraded because specific enzymes are lacking is released from the cell by exocytosis after an hour. Despite th ...
... types, it accumulates non-degradable material and retains it for the lifetime of the cell and organism. In amoebae, endocytosed cargo transits through the cell. Any material that cannot be degraded because specific enzymes are lacking is released from the cell by exocytosis after an hour. Despite th ...
AQA GCSE (9-1)
... calculating magnifications. Students will describe how cells divide by mitosis and applications of this in cloning techniques. The use of stem cells in producing new tissues and organs will be evaluated in terms of ethical and moral considerations. Students will consider the differences between aero ...
... calculating magnifications. Students will describe how cells divide by mitosis and applications of this in cloning techniques. The use of stem cells in producing new tissues and organs will be evaluated in terms of ethical and moral considerations. Students will consider the differences between aero ...
Expression and activity of platelet-derived growth factor receptor
... Cell culture. HepG2 cells were cultured on Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 100 U/ml penicilin, 100 µg/ml streptomycin and 10% fetal bovine serum (Biochrom, Germany). The HepG2 cell line, which does not express endogenous PDGF receptor-β, was used to create a cell line to ...
... Cell culture. HepG2 cells were cultured on Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 100 U/ml penicilin, 100 µg/ml streptomycin and 10% fetal bovine serum (Biochrom, Germany). The HepG2 cell line, which does not express endogenous PDGF receptor-β, was used to create a cell line to ...
New Views on the Plant Cytoskeleton
... tagged cytoskeletal elements. However, care still needs to be exercised when undertaking such work. Placing live samples on horizontal stages can elicit touch and gravity responses that are likely to alter cytoskeletal dynamics and organization. Incorporation into or decoration by live probes will p ...
... tagged cytoskeletal elements. However, care still needs to be exercised when undertaking such work. Placing live samples on horizontal stages can elicit touch and gravity responses that are likely to alter cytoskeletal dynamics and organization. Incorporation into or decoration by live probes will p ...
Exploring Bioinorganic Pattern Formation in Diatoms. A Story of
... pleuralin-1, is precisely restricted to the most terminal girdle band (known as the pleural band) of the epitheca (Fig. 2), in the region of overlap with the hypotheca. During cell division it also becomes associated with the pleural band of the hypotheca, at a time when the parental hypotheca is fu ...
... pleuralin-1, is precisely restricted to the most terminal girdle band (known as the pleural band) of the epitheca (Fig. 2), in the region of overlap with the hypotheca. During cell division it also becomes associated with the pleural band of the hypotheca, at a time when the parental hypotheca is fu ...
Biology Class IX for SA-I 2016-17
... The cell membrane is selectively permeable and regulates themovement of substances in and out of the cell. Movement of CO2: CO2 is produced during cellular respiration. Therefore, it is present in high concentrations inside the cell. This CO2 must be excreted out of the cell. In the cell’s external ...
... The cell membrane is selectively permeable and regulates themovement of substances in and out of the cell. Movement of CO2: CO2 is produced during cellular respiration. Therefore, it is present in high concentrations inside the cell. This CO2 must be excreted out of the cell. In the cell’s external ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.