localization of products of atp hydrolysis in mammalian smooth
... several changes were made in the constitution of the medium and in the incubating conditions. These modifications of the Wachstein-Meisel protocol for ATPase demonstration (16) were predicated on the hypothesis that the ATPase in smooth muscle has relatively low activity, requiring deviations from t ...
... several changes were made in the constitution of the medium and in the incubating conditions. These modifications of the Wachstein-Meisel protocol for ATPase demonstration (16) were predicated on the hypothesis that the ATPase in smooth muscle has relatively low activity, requiring deviations from t ...
Beginnings of a Good Apoptotic Meal: The Find-Me and
... through phagolysosomal processing (Kinchen et al., 2008; Kinchen and Ravichandran, 2008; Park et al., 2011; Zhou and Yu, 2008). In recent years, several new discoveries have been reported on all of the above aspects of engulfment. This review specifically covers recent discoveries that pertain to fi ...
... through phagolysosomal processing (Kinchen et al., 2008; Kinchen and Ravichandran, 2008; Park et al., 2011; Zhou and Yu, 2008). In recent years, several new discoveries have been reported on all of the above aspects of engulfment. This review specifically covers recent discoveries that pertain to fi ...
Triton X-100 Extraction of P815 Tumor Cells
... In contrast, many nucleated cells have extensive cytoskeletal systems that include microtubules, microfilaments, and intermediate filaments (13, 14). There is some evidence for associations between transmembrane proteins and these cytoskeletal elements but it has remained unclear whether the plasma ...
... In contrast, many nucleated cells have extensive cytoskeletal systems that include microtubules, microfilaments, and intermediate filaments (13, 14). There is some evidence for associations between transmembrane proteins and these cytoskeletal elements but it has remained unclear whether the plasma ...
Extracellular Trapping of Soil Contaminants by Root Border Cells
... Abstract: Soil and water pollution by metals and other toxic chemicals is difficult to measure and control, and, as such, presents an ongoing global threat to sustainable agriculture and human health. Efforts to remove contaminants by plant-mediated pathways, or “phytoremediation”, though widely stu ...
... Abstract: Soil and water pollution by metals and other toxic chemicals is difficult to measure and control, and, as such, presents an ongoing global threat to sustainable agriculture and human health. Efforts to remove contaminants by plant-mediated pathways, or “phytoremediation”, though widely stu ...
m5zn_7e2104c47c4f1d9
... Filtration another passive process for moving materials in and out of cell. Filtration, is the process by which movement of solvents “such as water” and solutes “such as glucose” across a selectively permeable membrane as a result of gravity or hydrostatic “water” pressure from an area of higher to ...
... Filtration another passive process for moving materials in and out of cell. Filtration, is the process by which movement of solvents “such as water” and solutes “such as glucose” across a selectively permeable membrane as a result of gravity or hydrostatic “water” pressure from an area of higher to ...
Comparative Biochemistry of the Oxidative Burst Produced by Rose
... An oxidative burst is a common response of plant cells to physical or biological stress. The production of ROS such as superoxide and H2O2 has been noted when plants are challenged with particular viral, bacterial, or fungal pathogens (Mehdy, 1994; Low and Merida, 1996; Wojtaszek, 1997; Bolwell and ...
... An oxidative burst is a common response of plant cells to physical or biological stress. The production of ROS such as superoxide and H2O2 has been noted when plants are challenged with particular viral, bacterial, or fungal pathogens (Mehdy, 1994; Low and Merida, 1996; Wojtaszek, 1997; Bolwell and ...
Ca - AJP - Renal Physiology - American Journal of Physiology
... The other difference between RNCX and SNCX occurs within the cytosolic loop at the alternative splice site. This site is encoded by 6 different exons denoted A-F (16, 29). In the RNCX clone, the alternative splice site is encoded by exons B and D, and in SNCX it is encoded by exons B, D, and F (33). ...
... The other difference between RNCX and SNCX occurs within the cytosolic loop at the alternative splice site. This site is encoded by 6 different exons denoted A-F (16, 29). In the RNCX clone, the alternative splice site is encoded by exons B and D, and in SNCX it is encoded by exons B, D, and F (33). ...
Generation of CD19-chimeric antigen receptor
... with retroviruses that encode either a truncated cell-surface CD19 molecule or full-length HLA-A*0201 or B*0702 (Phoenix-Ampho system; Orbigen), and sorted twice to obtain cells of ⬎ 95% purity that expressed CD19 or HLA class I, respectively (supplemental Figure 1, available on the Blood Web site; ...
... with retroviruses that encode either a truncated cell-surface CD19 molecule or full-length HLA-A*0201 or B*0702 (Phoenix-Ampho system; Orbigen), and sorted twice to obtain cells of ⬎ 95% purity that expressed CD19 or HLA class I, respectively (supplemental Figure 1, available on the Blood Web site; ...
A Model for Cell Proliferation in a Developing Organism
... Proliferation. A cell is selected uniformly at random. If the cell at site i is selected, it moves to site i + 1 pushing all cells to the right of it up by one. A new unmarked cell now occupies site i. Times between proliferation events are iid exp(λ). Equivalently. You can think of independent homo ...
... Proliferation. A cell is selected uniformly at random. If the cell at site i is selected, it moves to site i + 1 pushing all cells to the right of it up by one. A new unmarked cell now occupies site i. Times between proliferation events are iid exp(λ). Equivalently. You can think of independent homo ...
Terminal Cytokinesis Events Uncovered after an RNAi Screen
... separation of daughter cells. Rather, as first described by Flemming in 1891 (see [7]), a persistent intercellular bridge forms around the spindle remnant; this bridge is marked at its center by a darkly staining structure, or midbody (e.g., Figure 1A, insert). This bridge remains long after furrowi ...
... separation of daughter cells. Rather, as first described by Flemming in 1891 (see [7]), a persistent intercellular bridge forms around the spindle remnant; this bridge is marked at its center by a darkly staining structure, or midbody (e.g., Figure 1A, insert). This bridge remains long after furrowi ...
Introduction - UniMAP Portal
... Growth of biomolecules usually by bacterial or mammalian cell lines & harvest - BIOREACTOR ...
... Growth of biomolecules usually by bacterial or mammalian cell lines & harvest - BIOREACTOR ...
localization of products of atp hydrolysis in
... several changes were made in the constitution of the medium and in the incubating conditions. These modifications of the Wachstein-Meisel protocol for ATPase demonstration (16) were predicated on the hypothesis that the ATPase in smooth muscle has relatively low activity, requiring deviations from t ...
... several changes were made in the constitution of the medium and in the incubating conditions. These modifications of the Wachstein-Meisel protocol for ATPase demonstration (16) were predicated on the hypothesis that the ATPase in smooth muscle has relatively low activity, requiring deviations from t ...
Transforming growth factor‐β1 suppresses hepatitis B virus
... replication noncytopathically. Clinical observations indeed have revealed several cytokines to be elevated during the progression of hepatitis.8 These cytokines are likely to be involved in the regulation of immune responses against viral infection and may directly inhibit viral replication. Recentl ...
... replication noncytopathically. Clinical observations indeed have revealed several cytokines to be elevated during the progression of hepatitis.8 These cytokines are likely to be involved in the regulation of immune responses against viral infection and may directly inhibit viral replication. Recentl ...
Raven/Johnson Biology 8e
... b. Therapeutic cloning produces an embryo that can be implanted into a uterus. c. Therapeutic cloning produces whole tissues or organs. d. Therapeutic cloning provides a source of proteins. The correct answer is a—Therapeutic cloning provides a source of embryonic stem cells. A. Answer a is correct. ...
... b. Therapeutic cloning produces an embryo that can be implanted into a uterus. c. Therapeutic cloning produces whole tissues or organs. d. Therapeutic cloning provides a source of proteins. The correct answer is a—Therapeutic cloning provides a source of embryonic stem cells. A. Answer a is correct. ...
Raven/Johnson Biology 8e Chapter 19 Answers 1.
... b. Therapeutic cloning produces an embryo that can be implanted into a uterus. c. Therapeutic cloning produces whole tissues or organs. d. Therapeutic cloning provides a source of proteins. The correct answer is a—Therapeutic cloning provides a source of embryonic stem cells. A. Answer a is correct. ...
... b. Therapeutic cloning produces an embryo that can be implanted into a uterus. c. Therapeutic cloning produces whole tissues or organs. d. Therapeutic cloning provides a source of proteins. The correct answer is a—Therapeutic cloning provides a source of embryonic stem cells. A. Answer a is correct. ...
Identification of Amino Acid Residues within GABAA Receptor
... expression of homomeric b subunits. Live A293 cells were labeled by immunofluorescence using FL AG antibody followed by an Alexa 488-conjugated secondary antibody and analyzed by FACS. Figure 4 A shows typical results for mock-transfected A293 cells or cells expressing (FL AG)b2 or (FL AG)b3. E xpre ...
... expression of homomeric b subunits. Live A293 cells were labeled by immunofluorescence using FL AG antibody followed by an Alexa 488-conjugated secondary antibody and analyzed by FACS. Figure 4 A shows typical results for mock-transfected A293 cells or cells expressing (FL AG)b2 or (FL AG)b3. E xpre ...
FRET Applications to IOn Channels
... that has a known pharmacological activity. Because the probe occupies a known binding site, the technique is most effective when detecting molecules that bind to the same site. Although other sites are often allosterically coupled to the ligand site, these assays are not ideal for probing weakly cou ...
... that has a known pharmacological activity. Because the probe occupies a known binding site, the technique is most effective when detecting molecules that bind to the same site. Although other sites are often allosterically coupled to the ligand site, these assays are not ideal for probing weakly cou ...
The Dielectric Response of Spherical Live Cells in Suspension: An
... Dielectric spectroscopy has been successfully used in the past to characterize biological matter (1–8). These types of measurements probe the collective dielectric response of many live cells in suspensions or in tissues, but by using more or less elaborated models, useful information about the stat ...
... Dielectric spectroscopy has been successfully used in the past to characterize biological matter (1–8). These types of measurements probe the collective dielectric response of many live cells in suspensions or in tissues, but by using more or less elaborated models, useful information about the stat ...
Discreteness of chromosome territories
... metaphase chromosomes contain two chromatids labeled with IdUrd, incorporated into one strand of the DNA double helix (thick gray lines). (B) After a second S-phase in the presence of CldUrd, metaphase chromosomes contain one chromatid labeled with both IdUrd and CldUrd, and one chromatid labeled by ...
... metaphase chromosomes contain two chromatids labeled with IdUrd, incorporated into one strand of the DNA double helix (thick gray lines). (B) After a second S-phase in the presence of CldUrd, metaphase chromosomes contain one chromatid labeled with both IdUrd and CldUrd, and one chromatid labeled by ...
Embryology (Animal
... Sea urchin eggs have small amounts of yolk. The yolk tends to be in the lower part of the egg. This is called the “vegetal” half. The opposite end is called the “animal” half. The first cleavage and second cleavages of the sea urchin egg cut through both the animal and the vegetal poles of the egg. ...
... Sea urchin eggs have small amounts of yolk. The yolk tends to be in the lower part of the egg. This is called the “vegetal” half. The opposite end is called the “animal” half. The first cleavage and second cleavages of the sea urchin egg cut through both the animal and the vegetal poles of the egg. ...
Midbodies and phragmoplasts: analogous structures
... anaphase. It can have roles in spindle elongation and cytokinesis in various organisms. The microtubules are most likely remnants of the mitotic spindle that were not attached to chromosomes. Midbody: a compact, dense matrix of proteins embedded in the region of microtubule overlap that is formed fr ...
... anaphase. It can have roles in spindle elongation and cytokinesis in various organisms. The microtubules are most likely remnants of the mitotic spindle that were not attached to chromosomes. Midbody: a compact, dense matrix of proteins embedded in the region of microtubule overlap that is formed fr ...
A Golgi and tonoplast localized Sacyl transferase is involved in cell
... cells and poor viability when grown at 37°C. At 25°C near normal growth occurs (Feng & Davis, 2000). To determine if AtPAT10 is an S-acyl transferase, AtPAT10 and its point mutated variant AtPAT10C192A were expressed in akr1 yeast cells. Figure 1(a) shows that at the nonpermissive temperature of 37° ...
... cells and poor viability when grown at 37°C. At 25°C near normal growth occurs (Feng & Davis, 2000). To determine if AtPAT10 is an S-acyl transferase, AtPAT10 and its point mutated variant AtPAT10C192A were expressed in akr1 yeast cells. Figure 1(a) shows that at the nonpermissive temperature of 37° ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.