Sarcomere assembly in C. elegans muscle
... filaments to the cell membrane. Within each pathway, location of one protein above another with an intervening arrow means that the top protein is recruited to attachments independent of the protein below, or any of the other proteins further down the pathway. Reciprocally, the recruitment of the pr ...
... filaments to the cell membrane. Within each pathway, location of one protein above another with an intervening arrow means that the top protein is recruited to attachments independent of the protein below, or any of the other proteins further down the pathway. Reciprocally, the recruitment of the pr ...
Role of - Molecular Physiology and Biophysics
... experiment. Two additional experiments gave similar results. (B) Diagram showing the fusion proteins of a-DG used in this study. Two segments corresponding to the a-DG core protein, except amino acid residues 30 to 61, were expressed as glutathione S-transferase (GST ) fusion proteins (6). Fusion pr ...
... experiment. Two additional experiments gave similar results. (B) Diagram showing the fusion proteins of a-DG used in this study. Two segments corresponding to the a-DG core protein, except amino acid residues 30 to 61, were expressed as glutathione S-transferase (GST ) fusion proteins (6). Fusion pr ...
Chemotaxis in Bacteria
... as the concentration of attractant changes, the tumbling frequency is measured(25). [For a history of the use of temporal stimulation in the study of bacterial behavior, see the introduction to (25).] The fact that bacteria can "remember"that there is different concentration nowthan before has led t ...
... as the concentration of attractant changes, the tumbling frequency is measured(25). [For a history of the use of temporal stimulation in the study of bacterial behavior, see the introduction to (25).] The fact that bacteria can "remember"that there is different concentration nowthan before has led t ...
Life 9e - Garvness
... Textbook Reference: 16.3 How Is Eukaryotic Gene Transcription Regulated? Page: 353 Bloom’s Category: 2. Understanding 49. There are several proteins involved in transcription, including transcription factors, regulatory binding proteins, and activators. Their role is to tell RNA polymerase a. where ...
... Textbook Reference: 16.3 How Is Eukaryotic Gene Transcription Regulated? Page: 353 Bloom’s Category: 2. Understanding 49. There are several proteins involved in transcription, including transcription factors, regulatory binding proteins, and activators. Their role is to tell RNA polymerase a. where ...
Transcription Factor Veracity: 1s GBF3 Responsible for ABA
... GBF by using the G-box found within the context of the Adh promoter as a probe in a standard protein-DNA interaction screen. Although the cDNA clones extended existing information on GBF3, we failed to find any new or unique GBFs due to any novel features that the Adh G-box might have. However, by c ...
... GBF by using the G-box found within the context of the Adh promoter as a probe in a standard protein-DNA interaction screen. Although the cDNA clones extended existing information on GBF3, we failed to find any new or unique GBFs due to any novel features that the Adh G-box might have. However, by c ...
Role of Chitinase and Other Lysosomal Enzymes of
... vegetative mycelium appear to be chemically similar : thin layer chromatography of hydrolysates revealed only glucosamine. If isolated walls were incubated with autolysate liquor, N-acetylglucosamine was formed. Thus, chitin is a prominent wall constituent which has, in fact, been identified by Frey ...
... vegetative mycelium appear to be chemically similar : thin layer chromatography of hydrolysates revealed only glucosamine. If isolated walls were incubated with autolysate liquor, N-acetylglucosamine was formed. Thus, chitin is a prominent wall constituent which has, in fact, been identified by Frey ...
Gumbo JR and Cloete TE
... process. Also the B. mycoides species formed clumps, possible as a survival strategy in the event of nutrient limitations (Villain et al., 2006). It was very difficult to break these clumps except through ultrasonication. Some Bacillus species were reported to revert to spore formation, only germina ...
... process. Also the B. mycoides species formed clumps, possible as a survival strategy in the event of nutrient limitations (Villain et al., 2006). It was very difficult to break these clumps except through ultrasonication. Some Bacillus species were reported to revert to spore formation, only germina ...
5 | structure and function of plasma membranes
... interaction with its environment (see Table 5.1 for a summary). Cells exclude some substances, take in others, and excrete still others, all in controlled quantities. The plasma membrane must be very flexible to allow certain cells, such as red blood cells and white blood cells, to change shape as t ...
... interaction with its environment (see Table 5.1 for a summary). Cells exclude some substances, take in others, and excrete still others, all in controlled quantities. The plasma membrane must be very flexible to allow certain cells, such as red blood cells and white blood cells, to change shape as t ...
PDF - Bentham Open
... Abstract: Rationale: Mucus hypersecretion has been shown to be an important cause of airway obstruction in COPD and asthma, and has been associated with fatal asthma. Previous in-vitro and in-vivo studies have provided direct evidence that the myristoylated alanine-rich C kinase substrate (MARCKS) i ...
... Abstract: Rationale: Mucus hypersecretion has been shown to be an important cause of airway obstruction in COPD and asthma, and has been associated with fatal asthma. Previous in-vitro and in-vivo studies have provided direct evidence that the myristoylated alanine-rich C kinase substrate (MARCKS) i ...
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... timed experiments. Identified cells in 285 cleavagestage embryos (stages l-6c) were examined; the lengths and compositions of their cell cycles are presented in Figs 3 and 4. Limitations to the accuracy of our data are as follows: (1) Embryos were fixed at 30min intervals; thus, if homologous cells ...
... timed experiments. Identified cells in 285 cleavagestage embryos (stages l-6c) were examined; the lengths and compositions of their cell cycles are presented in Figs 3 and 4. Limitations to the accuracy of our data are as follows: (1) Embryos were fixed at 30min intervals; thus, if homologous cells ...
ATP-binding-cassette (ABC) transport systems: Functional and
... factor Tu) [30], and that of adenylate kinase only [1], respectively. According to these predictions, the consensus fragment of all ABC domains (approx. 250 residues) is folded in an alternating series of six K-helical subdomains and ¢ve L-strands. The Walker motifs A (K-helical, preceded by a glyci ...
... factor Tu) [30], and that of adenylate kinase only [1], respectively. According to these predictions, the consensus fragment of all ABC domains (approx. 250 residues) is folded in an alternating series of six K-helical subdomains and ¢ve L-strands. The Walker motifs A (K-helical, preceded by a glyci ...
Cellular lipidomics
... even within the lateral plane of the membrane. To incorporate function, cellular lipidomics must not only determine which lipids are present but also the concentration of each lipid at each specific intracellular location in time and the lipid’s interaction partners. Moreover, cellular lipidomics mus ...
... even within the lateral plane of the membrane. To incorporate function, cellular lipidomics must not only determine which lipids are present but also the concentration of each lipid at each specific intracellular location in time and the lipid’s interaction partners. Moreover, cellular lipidomics mus ...
Neuroprotective Effects of 17Я-Estradiol and Nonfeminizing
... have been demonstrated in a variety of models of acute cerebral ischemia. These include transient and permanent middle cerebral artery occlusion models (Simpkins et al., 1997; Alkayed et al., 1998; Dubal et al., 1998), global forebrain ischemia models (Sudo et al., 1997), photothrombotic focal ische ...
... have been demonstrated in a variety of models of acute cerebral ischemia. These include transient and permanent middle cerebral artery occlusion models (Simpkins et al., 1997; Alkayed et al., 1998; Dubal et al., 1998), global forebrain ischemia models (Sudo et al., 1997), photothrombotic focal ische ...
Retinoid signaling regulates primitive (yolk sac
... genes that are required for definitive hematopoiesis. For example, definitive but not primitive erythropoiesis is critically dependent on erythropoietin.3 Vitamin A-derived retinoids, including retinoic acid (RA), are required for normal embryogenesis and tissue maintenance.4 Retinoids function as s ...
... genes that are required for definitive hematopoiesis. For example, definitive but not primitive erythropoiesis is critically dependent on erythropoietin.3 Vitamin A-derived retinoids, including retinoic acid (RA), are required for normal embryogenesis and tissue maintenance.4 Retinoids function as s ...
Eukaryotic origins of DNA replication: could you please be more
... Precise duplication of the eukaryotic genome during each cell cycle is critically important for cellular survival. As such, mechanisms that control how replication initiates are highly regulated by the cell. Initial models of DNA duplication suggested that replication would begin when a sequence-spe ...
... Precise duplication of the eukaryotic genome during each cell cycle is critically important for cellular survival. As such, mechanisms that control how replication initiates are highly regulated by the cell. Initial models of DNA duplication suggested that replication would begin when a sequence-spe ...
How Have Plant Cell Walls Evolved?1
... many million years after the horsetails. This finding highlights the importance of wide-scale sampling that encompasses less-well-studied species and raises the question of whether or not the biosynthetic mechanisms of MLG biosynthesis are deeply conserved, or rather have emerged by convergent evolu ...
... many million years after the horsetails. This finding highlights the importance of wide-scale sampling that encompasses less-well-studied species and raises the question of whether or not the biosynthetic mechanisms of MLG biosynthesis are deeply conserved, or rather have emerged by convergent evolu ...
FROM THE BEAUTIFUL TO THE BIZARRE
... whether their forebears originated on land or in water, and the shape of their phylogenetic tree. But about one thing there can be no doubt: plants do the damndest things. A few cases in point: In 1999, the Massachusetts Museum of Contemporary Art (MASS MoCA) opened its doors. Much about the new com ...
... whether their forebears originated on land or in water, and the shape of their phylogenetic tree. But about one thing there can be no doubt: plants do the damndest things. A few cases in point: In 1999, the Massachusetts Museum of Contemporary Art (MASS MoCA) opened its doors. Much about the new com ...
A rapid and robust assay for detection of S
... effectively be determined by flow cytometry after isolation of nuclei. In animal cell cultures, high quality and intact nuclei preparations can be relatively easily obtained in hypotonic buffers with detergents. In plants, however, cell walls pose a significant barrier for nuclei isolation. To allev ...
... effectively be determined by flow cytometry after isolation of nuclei. In animal cell cultures, high quality and intact nuclei preparations can be relatively easily obtained in hypotonic buffers with detergents. In plants, however, cell walls pose a significant barrier for nuclei isolation. To allev ...
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... protrusions, they were plotted as falling into one of 18 sectors of 20 degrees each around the cell perimeter, with 90°and 270° representing medial and lateral positions. Cell shapes were traced from images on the video monitor and digitized on a Summasketch graphics tablet (Summagraphics, Inc.). Ar ...
... protrusions, they were plotted as falling into one of 18 sectors of 20 degrees each around the cell perimeter, with 90°and 270° representing medial and lateral positions. Cell shapes were traced from images on the video monitor and digitized on a Summasketch graphics tablet (Summagraphics, Inc.). Ar ...
Peeping in on the cytoskeleton: light microscopy
... spatio-temporal specificity. The relative regional autonomy of polymer assembly/disassembly and supramolecular organization endows the cytoskeletal systems with structural specificity, which in the context of cell biology is translated to functional specificity7,8. The length and timescales these sy ...
... spatio-temporal specificity. The relative regional autonomy of polymer assembly/disassembly and supramolecular organization endows the cytoskeletal systems with structural specificity, which in the context of cell biology is translated to functional specificity7,8. The length and timescales these sy ...
An Enhanced System for Unnatural Amino Acid
... was effective in decreasing protein truncation and in increasing overall yields of mutant proteins.21,27 A plasmid with a mid-copy-number to low-copynumber p15A origin27,28 was chosen to encode the Tyr pair. This origin allows target genes aaRS/tRNACUA of interest to be harbored in compatible colE1 ...
... was effective in decreasing protein truncation and in increasing overall yields of mutant proteins.21,27 A plasmid with a mid-copy-number to low-copynumber p15A origin27,28 was chosen to encode the Tyr pair. This origin allows target genes aaRS/tRNACUA of interest to be harbored in compatible colE1 ...
studies on the intracellular digestive process in mammalian tissue
... found within phagocytotic vacuoles formed by the invagination of the cell membrane (1). These vacuoles were shown to migrate toward the Golgi zone (1) and were most frequently observed in this region (Figs. 1 and 2). After extensive phagocytosis, the Golgi apparatus appeared more prominent owing to ...
... found within phagocytotic vacuoles formed by the invagination of the cell membrane (1). These vacuoles were shown to migrate toward the Golgi zone (1) and were most frequently observed in this region (Figs. 1 and 2). After extensive phagocytosis, the Golgi apparatus appeared more prominent owing to ...
Sample pages 1 PDF
... growth regulators, mainly auxins (Altamura et al. 2016). It is interesting that many species require an initial shot of auxins, but thereafter the auxin must be degraded for SE to proceed (Chap. 10) (Altamura et al. 2016). Clearly the onset of SE depends on a complex network of interactions among pl ...
... growth regulators, mainly auxins (Altamura et al. 2016). It is interesting that many species require an initial shot of auxins, but thereafter the auxin must be degraded for SE to proceed (Chap. 10) (Altamura et al. 2016). Clearly the onset of SE depends on a complex network of interactions among pl ...
A Role for Eosinophils in Adaptive Humoral Immunity
... interleukins, chemokines and RNAses (collectively known as eokines), which can be rapidly released during an inflammatory response to foreign proteins. Their accumulation and release of diverse eokines during the inflammation that accompanies an anamnestic response suggests an immunoregulatory funct ...
... interleukins, chemokines and RNAses (collectively known as eokines), which can be rapidly released during an inflammatory response to foreign proteins. Their accumulation and release of diverse eokines during the inflammation that accompanies an anamnestic response suggests an immunoregulatory funct ...
(pdf-file 1,2 Mb)
... Enp1 and Rps3 from the 40S pre-ribosome is regulated by the Hrr25 protein kinase. To determine whether Hrr25 controls Rps3 assembly in vivo, 40S subunits were isolated from the Hrr25-depleted cells by sucrosegradient centrifugation and treated with a high salt concentration. Rps3 was released from t ...
... Enp1 and Rps3 from the 40S pre-ribosome is regulated by the Hrr25 protein kinase. To determine whether Hrr25 controls Rps3 assembly in vivo, 40S subunits were isolated from the Hrr25-depleted cells by sucrosegradient centrifugation and treated with a high salt concentration. Rps3 was released from t ...