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... 1980). Stock solutions of sterols at 50 mg/ml benzene were diluted into 5 % BSA in 0-14 M-NaCl, pH 7 0 , vortexed and cleared by centrifugation to give a sterol solution of 500/*g/ml. This solution was then diluted into M16 + 2 % foetal calf serum (M16 + FCS) to give the appropriate sterol concentra ...
... 1980). Stock solutions of sterols at 50 mg/ml benzene were diluted into 5 % BSA in 0-14 M-NaCl, pH 7 0 , vortexed and cleared by centrifugation to give a sterol solution of 500/*g/ml. This solution was then diluted into M16 + 2 % foetal calf serum (M16 + FCS) to give the appropriate sterol concentra ...
1 - AState.edu
... • The endoplasmic reticulum (ER) is one of the main manufacturing facilities in a cell. • The ER • produces an enormous variety of molecules, • is connected to the nuclear envelope, and • is composed of interconnected rough and smooth ER that have different structures and functions. ...
... • The endoplasmic reticulum (ER) is one of the main manufacturing facilities in a cell. • The ER • produces an enormous variety of molecules, • is connected to the nuclear envelope, and • is composed of interconnected rough and smooth ER that have different structures and functions. ...
Human Corneal Endothelial Cells Expanded In Vitro Are a Powerful
... to Disruption of Cell-Cell Junctions Is the Obstacle for in Vitro Growth of HCECs Contact-inhibition is present in non-transformed normal cells when neighboring cells are in contact with one another. This fact causes low regenerative capability of in vivo human corneal endothelial cells. The convent ...
... to Disruption of Cell-Cell Junctions Is the Obstacle for in Vitro Growth of HCECs Contact-inhibition is present in non-transformed normal cells when neighboring cells are in contact with one another. This fact causes low regenerative capability of in vivo human corneal endothelial cells. The convent ...
Upstream-binding factor is sequestered into herpes simplex virus
... different levels (Hiscox, 2007). We recently showed that adenovirus, which has a linear double-stranded DNA genome, interacts with the nucleolar component called upstream-binding factor (UBF). UBF is normally associated with rDNA at the rRNA promoters and plays a role in the recruitment of RNA pol I ...
... different levels (Hiscox, 2007). We recently showed that adenovirus, which has a linear double-stranded DNA genome, interacts with the nucleolar component called upstream-binding factor (UBF). UBF is normally associated with rDNA at the rRNA promoters and plays a role in the recruitment of RNA pol I ...
Therapies for sarcopenia and regeneration of old skeletal muscles
... formation of myotubes at 7 d in any grafts. Subsequent crosstransplantation studies using much older mice (female C57Bl/6J) aged 27–29 mo13 showed a marked delay in inflammation (and hence no initiation of myogenesis) at 5 d after transplantation for old autografts, a time when new myotubes were con ...
... formation of myotubes at 7 d in any grafts. Subsequent crosstransplantation studies using much older mice (female C57Bl/6J) aged 27–29 mo13 showed a marked delay in inflammation (and hence no initiation of myogenesis) at 5 d after transplantation for old autografts, a time when new myotubes were con ...
Saccharomyces cerevisiae adaptation to weak acids involves the
... deletion mutant strains to inhibitory concentrations of the different weak organic acids was compared by spot assays or by comparing the growth curves and the concentration of viable cells in MM4 liquid medium (pH 4.0). In spot assays, the cell suspensions used to inoculate the agar plates were prepa ...
... deletion mutant strains to inhibitory concentrations of the different weak organic acids was compared by spot assays or by comparing the growth curves and the concentration of viable cells in MM4 liquid medium (pH 4.0). In spot assays, the cell suspensions used to inoculate the agar plates were prepa ...
Inglés
... It has been also observed that phages release their progeny using their own lytic enzymes, which, in the pneumococcal system, have features common to the lytic enzymes of the bacterium, such as the dependence of the lytic enzymes of both the host and its phage on the presence of choline in teichoic ...
... It has been also observed that phages release their progeny using their own lytic enzymes, which, in the pneumococcal system, have features common to the lytic enzymes of the bacterium, such as the dependence of the lytic enzymes of both the host and its phage on the presence of choline in teichoic ...
Cytoplasmic streaming enables the distribution of molecules
... physiological consequences of streaming fall naturally into the two categories of intra- and intercellular roles. These potential roles are easily seen using as a model the alga, Chara corallina, which has historically been among the organisms of choice for studies of cytoplasmic streaming due to it ...
... physiological consequences of streaming fall naturally into the two categories of intra- and intercellular roles. These potential roles are easily seen using as a model the alga, Chara corallina, which has historically been among the organisms of choice for studies of cytoplasmic streaming due to it ...
FREE Sample Here
... Ans: Living organisms (1) are chemically complex and highly organized; (2) extract, transform, and use energy from their environment; (3) have the capacity to precisely self-replicate and self-assemble; (4) exploit a chemical interplay with their environment; (5) possess programmatically defined fun ...
... Ans: Living organisms (1) are chemically complex and highly organized; (2) extract, transform, and use energy from their environment; (3) have the capacity to precisely self-replicate and self-assemble; (4) exploit a chemical interplay with their environment; (5) possess programmatically defined fun ...
FREE Sample Here
... Ans: Living organisms (1) are chemically complex and highly organized; (2) extract, transform, and use energy from their environment; (3) have the capacity to precisely self-replicate and self-assemble; (4) exploit a chemical interplay with their environment; (5) possess programmatically defined fun ...
... Ans: Living organisms (1) are chemically complex and highly organized; (2) extract, transform, and use energy from their environment; (3) have the capacity to precisely self-replicate and self-assemble; (4) exploit a chemical interplay with their environment; (5) possess programmatically defined fun ...
Cytoplasmic streaming enables the distribution of molecules
... physiological consequences of streaming fall naturally into the two categories of intra- and intercellular roles. These potential roles are easily seen using as a model the alga, Chara corallina, which has historically been among the organisms of choice for studies of cytoplasmic streaming due to it ...
... physiological consequences of streaming fall naturally into the two categories of intra- and intercellular roles. These potential roles are easily seen using as a model the alga, Chara corallina, which has historically been among the organisms of choice for studies of cytoplasmic streaming due to it ...
Sample
... 1. Unless delivered intravenously, a drug must cross several semipermeable cell membranes before it reaches the systemic circulation. Identify the different mechanisms used by a molecule to cross a cell membrane. Depending upon its properties, a molecule may move into a cell via diffusion, facilitat ...
... 1. Unless delivered intravenously, a drug must cross several semipermeable cell membranes before it reaches the systemic circulation. Identify the different mechanisms used by a molecule to cross a cell membrane. Depending upon its properties, a molecule may move into a cell via diffusion, facilitat ...
Protist Characteristics
... helps the cell move; also in some protists. • motility: The ability to move. • pseudopod: Temporary, foot-like extension of the cytoplasm that some cells use for movement or feeding. • spore: A reproductive cell produced by various organisms; can withstand harsh environmental conditions. • symbiosis ...
... helps the cell move; also in some protists. • motility: The ability to move. • pseudopod: Temporary, foot-like extension of the cytoplasm that some cells use for movement or feeding. • spore: A reproductive cell produced by various organisms; can withstand harsh environmental conditions. • symbiosis ...
Removal of SDS from biological protein digests for proteomic
... the suitable selection of their building blocks (metal and organic linker). Apart from these features, the lower acidity of the active centers in MOFs makes these materials even very attractive compared Zeolites (highly acidic centers). Also, MOFs may be changing the interactions of adsorbing reacta ...
... the suitable selection of their building blocks (metal and organic linker). Apart from these features, the lower acidity of the active centers in MOFs makes these materials even very attractive compared Zeolites (highly acidic centers). Also, MOFs may be changing the interactions of adsorbing reacta ...
The polar flagellum mediates Azuspidlum brasifense
... plate. The bacteria were pelleted by centrifugation (4000 r.p.m., 15 min) and resuspended in 100ml of the same bufler. This suspension was mixcd for 40 s at maximum speed in a Waring Blender to break the flagella. The bacteria and bacterial cell debris were removed from the mixed cell suspension by ...
... plate. The bacteria were pelleted by centrifugation (4000 r.p.m., 15 min) and resuspended in 100ml of the same bufler. This suspension was mixcd for 40 s at maximum speed in a Waring Blender to break the flagella. The bacteria and bacterial cell debris were removed from the mixed cell suspension by ...
Feature selection using a one dimensional naıve
... stimulates innate immunity and hence drives effective antigen presentation via T cell co-stimulation (Ibrahim et al., 1995; Janeway, 1989). We therefore wished to extend our investigation to analyze the response to a well-studied model antigen, ovalbumin (OVA), when delivered in the context of CFA. ...
... stimulates innate immunity and hence drives effective antigen presentation via T cell co-stimulation (Ibrahim et al., 1995; Janeway, 1989). We therefore wished to extend our investigation to analyze the response to a well-studied model antigen, ovalbumin (OVA), when delivered in the context of CFA. ...
The impact of Spemann`s concepts on molecular embryology
... In 1927 Hans Spemann wrote in his article New work on the organizer: "What has been achieved is but the first step; we still stand in the presence of riddles, but not without hope of solving them. And riddles with the hope of solution-what more can a scientist desire?" The problem referred to, which ...
... In 1927 Hans Spemann wrote in his article New work on the organizer: "What has been achieved is but the first step; we still stand in the presence of riddles, but not without hope of solving them. And riddles with the hope of solution-what more can a scientist desire?" The problem referred to, which ...
Document
... - two examples with different gap penalties These values are copied from the PAM250 matrix (see earlier slide), after being made nonnegative by adding 8 to each PAM250 matrix cell (-8 is the lowest number in the PAM250 matrix) ...
... - two examples with different gap penalties These values are copied from the PAM250 matrix (see earlier slide), after being made nonnegative by adding 8 to each PAM250 matrix cell (-8 is the lowest number in the PAM250 matrix) ...
Parenchyma cells
... Parenchyma – Ground tissue Parenchyma cells contain a nucleus and retain the ability for future cell division. When they are first formed, they are densely cytoplasmic and have several small vacuoles. As the cells enlarge, the vacuole size increases and intercellular spaces can form between cell wal ...
... Parenchyma – Ground tissue Parenchyma cells contain a nucleus and retain the ability for future cell division. When they are first formed, they are densely cytoplasmic and have several small vacuoles. As the cells enlarge, the vacuole size increases and intercellular spaces can form between cell wal ...
Biology Unit Title: Basic Biological Principles Timeline (approximate
... cytokenesis, DNA Describe the events that occur replication, gene during meiotic and mitotic division recombination, interphase, meiosis, Compare the processes and simulation lab to compare mitosis mitosis, mutation, outcomes of mitotic and meiotic and meiosis nondisjunction, point nuclear divisions ...
... cytokenesis, DNA Describe the events that occur replication, gene during meiotic and mitotic division recombination, interphase, meiosis, Compare the processes and simulation lab to compare mitosis mitosis, mutation, outcomes of mitotic and meiotic and meiosis nondisjunction, point nuclear divisions ...
Proteolysis and orientation in Dictyostelium slugs
... the slug tip would take a sharp turn away from the side where the bead was placed (Fig. 2 a ; Table 1). Because it was known from previous work that papain digested the slime sheath (Wlutfield, 1964; Takeuchi & Yabuno, 1970) it is reasonable that in the experiment here the papain caused proteolysis ...
... the slug tip would take a sharp turn away from the side where the bead was placed (Fig. 2 a ; Table 1). Because it was known from previous work that papain digested the slime sheath (Wlutfield, 1964; Takeuchi & Yabuno, 1970) it is reasonable that in the experiment here the papain caused proteolysis ...
A Tertiary Plastid Uses Genes from Two Endosymbionts
... unique among eukaryotic algae in that they have taken the process of endosymbiosis one step further: in several independent lineages, the peridinin plastid has been replaced, either with a successive secondary plastid8 or with a plastid from another secondary alga, resulting in tertiary plastids.9–1 ...
... unique among eukaryotic algae in that they have taken the process of endosymbiosis one step further: in several independent lineages, the peridinin plastid has been replaced, either with a successive secondary plastid8 or with a plastid from another secondary alga, resulting in tertiary plastids.9–1 ...
Coombes JL, Han SJ, van Rooijen N, Raulet DH, Robey EA. 2012. Infection-induced regulation of natural killer cells by macrophages and collagen at the lymph node subcapsular sinus. Cell rep 2(1):124-135.
... observed that a substantial fraction of the slow-moving NK cells closely associated with second harmonic signals indicative of collagen fibers (Figure 3A; Movie S2). Antibody staining of lymph node tissue sections confirmed that these structures contained type I collagen (Figure 3B). Interactions be ...
... observed that a substantial fraction of the slow-moving NK cells closely associated with second harmonic signals indicative of collagen fibers (Figure 3A; Movie S2). Antibody staining of lymph node tissue sections confirmed that these structures contained type I collagen (Figure 3B). Interactions be ...
The Rx Gene from Potato Controls Separate Virus Resistance and
... that are themselves antibiotics or disinfectants (Lamb and Dixon, 1997). This latter view is supported by earlier studies on tobacco N gene–mediated resistance to tobacco mosaic virus (TMV). TMV particles could be found in cells surrounding the necrotic HR lesion, even when lesion expansion had stop ...
... that are themselves antibiotics or disinfectants (Lamb and Dixon, 1997). This latter view is supported by earlier studies on tobacco N gene–mediated resistance to tobacco mosaic virus (TMV). TMV particles could be found in cells surrounding the necrotic HR lesion, even when lesion expansion had stop ...
Mechanism of Inhibition of Cell Proliferation by
... "Discussion"]. This may be due to the fact that the Vinca alkaloids are concentrated intracellularly (14, 15) and accumu late differentially in some cells (14). Alternatively, one or all of the Vinca alkaloids may act on targets other than tubulin or microtubules. The Vinca alkaloids are known to af ...
... "Discussion"]. This may be due to the fact that the Vinca alkaloids are concentrated intracellularly (14, 15) and accumu late differentially in some cells (14). Alternatively, one or all of the Vinca alkaloids may act on targets other than tubulin or microtubules. The Vinca alkaloids are known to af ...