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The use of yeast inoculation in fermentation for port production
The use of yeast inoculation in fermentation for port production

... Must fermentations and sampling Thermovinified must was employed as the fermentation medium; this was prepared during the 1993 vintage by heating unfermented red must to approximately 70°C for 20 minutes, the resulting must (hexose sugar content of 210 g/l) was stored at -8°C until use. Four ferment ...
The δ Subunit of DNA Polymerase III Holoenzyme Serves as a
The δ Subunit of DNA Polymerase III Holoenzyme Serves as a

... new primer and clamp to continue DNA replication (Fig. 1) (17, 18). This model mechanism predicts that if ␤ remains stably associated with DNA, the clamps abandoned by the polymerase will accumulate along the lagging strand DNA. Indeed, in vitro rolling circle assays performed with pol III* detect i ...
Reviews
Reviews

... perfect, non interactive stuffing” (p 8 of ref 23). While more recent approaches to HA structure in aqueous solutions will be discussed later on, in this section it is important to remark that this view came to an end by 1972, with the discovery of proteins that can specifically interact with HA. In ...
Temporal Order of DNA Replication
Temporal Order of DNA Replication

... most of these replication domains have not yet been mapped at the molecular level, but the availability of extensive cloned contigs, as well as recent advances in technology, should now make it possible to examine these units in detail. The replication time of a genomic DNA fragment can be measured ...
Cortical cell fate specification
Cortical cell fate specification

... differentiated cell types that can mediate distinct functions. During development, differentiated cell types are generated from dividing progenitor cells. The mechanisms by which the principal cell types of the nervous system, neurons and glia, are generated from the progenitor population has been a ...
Morphine Modulates Mouse Hippocampal Progenitor Cell Lineages
Morphine Modulates Mouse Hippocampal Progenitor Cell Lineages

... that Notch1 inhibits neurogenesis by turning CSL-SKIP into an activator and stimulating the transcription of Hes1 and Hes5 [17]. Moreover, transient activation of Notch1 and Notch3 was able to induce an irreversible switch from NPCs to astrocytes in a way independently of STAT3 [18]. This ability to ...
Agrobacterium tumefaciens: a natural tool for plant transformation
Agrobacterium tumefaciens: a natural tool for plant transformation

... right side of the att locus resulted in the irreversible loss of attachment capacity, which could not be restored by conditioned medium (Bradley, 1997). These results suggest that genes, placed at att left side, are involved in molecular signaling events, while the right side genes are likely to be ...
Studying Cell–Surface Interactions In Vitro: A Survey of
Studying Cell–Surface Interactions In Vitro: A Survey of

... respect to their specificity for different ECM proteins. Molecular recognition and binding of individual ECM proteins are generally mediated by rather short amino acid sequences (*4 – 10 amino acids) within the primary structure of ECM proteins. The most well-known amino acid sequence involved in in ...
Neural differentiation from embryonic stem cells in vitro: An overview
Neural differentiation from embryonic stem cells in vitro: An overview

... SB431542, Chambers et al[5] shown that these two inhibitors of SMAD signaling are sufficient to allow the induction of neural differentiation derived from hESCs. Genome-wide mapping was used to obtain plausible downstream candidates within the TGFβ/BMP pathway that are involved in ESCs differentiati ...
Microbiology of the Rumen - Iowa State University: Animal Science
Microbiology of the Rumen - Iowa State University: Animal Science

... 500-20,000 α-(1, 4)-D-glucose units with a few α-1, 6 branches. Amylose can form an extended shape. Hydrogen bonding occurs between aligned chains. The aligned chains may form double stranded crystallites that are resistant to amylases. Amylopectin is formed by non-random α-1, 6 branching of the amy ...
Gamete Handling and Fertilization
Gamete Handling and Fertilization

... potential of the oocytes leading to a change in the osmosis, making the oocytes hypotonic, and the integrity of the membrane, making it more permeable to ASW attributing to a change in osmosis or the pressure within the oocytes. As in previous studies LiCl incubation interfered with cell division in ...
Cells 1.2
Cells 1.2

... Click to Launch: ...
Spirochaeta isovalerica sp. nov., a Marine Anaerobe That Forms
Spirochaeta isovalerica sp. nov., a Marine Anaerobe That Forms

... the above-mentioned branched-chain fatty acids are derived from fermentation of L-leucine, Lisoleucine, and L-valine. Although these amino acids do not serve as growth substrates for the spirochetes, the dissimilation of these compounds provides the organisms with maintenance energy for cell functio ...
PDF
PDF

... Kewley, 2004). Members of the bHLH-PAS protein family are characterized by an N-terminal bHLH domain required for DNA binding and dimerization, as well as by a PAS domain that acts as a secondary dimerization interface (Crews and Fan, 1999; Taylor and Zhulin, 1999). Transcriptional control of target ...
insider - The Paleo Diet
insider - The Paleo Diet

... like what we found with the Newman study to make a strong case for the notion that diet is responsible for acne, and that diet can ameliorate acne symptoms. We now have three epidemiologic studies and one clinical trial already supporting the role of diet in acne. We still need to work out all the m ...
Disruption of early proximodistal patterning and AVE
Disruption of early proximodistal patterning and AVE

... et al., 1999), demonstrating a clear role for Wnt signaling in mesendoderm development. However, genes expressed in the AVE are correctly localized (Liu et al., 1999), suggesting that Wnt3–/– embryos initiate AP axis formation and that Wnt3 acts downstream of an earlier AP induction signal. ␤-Cateni ...
Small-molecule binding sites to explore new targets in the cancer
Small-molecule binding sites to explore new targets in the cancer

... The transcription factor HNF4A forms a homodimer complex to interact with DNA to control the expression of other genes. In the monomer structure, two binding sites were detected on the protein surface (Fig. S5D). One of these two sites is bound to a saturated fatty acid in multiple superimposed crys ...
Microalgae Synthesize Hydrocarbons from Long
Microalgae Synthesize Hydrocarbons from Long

... Heptadecene Content Varies with Growth Stage and Culture Conditions ...
HIV1 impairs in vitro priming of nave T cells and gives rise to
HIV1 impairs in vitro priming of nave T cells and gives rise to

... equivalents) of whole inf-HIV or AT-2-HIV (CXCR4-tropic HIV-MN or CCR5-tropic HIV-1BaL) were impaired in their ability to prime naı̈ve T cells. We established an allogeneic primary culture system consisting of DC and naı̈ve bulk T cells. Inf-HIV-1 and AT-2 HIV-1 were compared to determine whether pr ...
unravelling the therapeutic potential of transmembrane peptides
unravelling the therapeutic potential of transmembrane peptides

... has  long  been  slowed  down  by  technical  hurdles  associated  with  producing,  purifying  and  analyzing  membrane‐embedded  proteins12.  To  circumvent  these  obstacles  a  “divide  and  conquer  approach”  was  proposed  in  which  a  complex,  multi  span  membrane  protein  is  broken  do ...
Quorum sensing, communication and cross
Quorum sensing, communication and cross

... main acyl chain donor (acyl-CoA derivatives can also be substrates), and S-adenosylmethionine as the source for the homoserine lactone moiety (Fig. 4). Since LuxI proteins are not particularly closely related it has been impossible to predict the nature of the AHL produced by a given LuxI protein fr ...
Adipocyte lipolysis links obesity to breast cancer growth: adipocyte
Adipocyte lipolysis links obesity to breast cancer growth: adipocyte

... highly relevant in the breast where adipocytes, professional lipid storage cells, are the predominant cell population and are capable of secreting significant quantities of metabolic substrates such as glycerol and fatty acids. Further, there is close juxtaposition of adipocytes and breast cancer ce ...
Mudskipper gill MR cell ion-transport proteins
Mudskipper gill MR cell ion-transport proteins

... MR cells have a tubular system continuous with the basolateral membrane that is typical of teleost fishes (Pisam, 1981; Philpott, 1980). However, the MR cells of this species have deep apical crypts, and accessory-type cells were seldom observed (Wilson et al., 1999). Accessory cells are typically a ...
Two Distinct Sources of Elicited Reactive Oxygen
Two Distinct Sources of Elicited Reactive Oxygen

... phase I production of ROS appears to involve an elicitorreceptor interaction, the responses are not always correlated with plant disease resistance. Later ROS production (many hours) is termed phase II and correlates with the resistance or susceptibility of the plant to the pathogen. Phase I and II ...
Vesicular stomatitis virus infects resident cells of the central nervous
Vesicular stomatitis virus infects resident cells of the central nervous

... (data not shown). As shown in Fig. 2A, VSV-associated GFP fluorescence was detectable in astrocytes by 12 h p.i. and was broadly expressed at 24 h p.i. We also detected cellular accumulation of VSV N and P proteins 24 h p.i. in both microglia and astrocytes (data not shown). VSV replication in astroc ...
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