progress and problems with the use of viral vectors for
... In April 2000, a paper was published in the journal Science that marked the highest point in the turbulent history of gene therapy. In a paper entitled “Gene therapy of human severe combined immunodeficiency (SCID)-XI disease”, Maria Cavazzana-Calvo, Alain Fischer and colleagues at the Necker Hospit ...
... In April 2000, a paper was published in the journal Science that marked the highest point in the turbulent history of gene therapy. In a paper entitled “Gene therapy of human severe combined immunodeficiency (SCID)-XI disease”, Maria Cavazzana-Calvo, Alain Fischer and colleagues at the Necker Hospit ...
Uptake of glutamate, not glutamine synthetase, regulates adaptation
... Glutamine synthetase activity during cell growth Changes in GS activity have been associated with the concentration of glutamine in the medium and these were investigated. The intracellular glutamine synthetase activity of McCoy (Fig. 3A) and MDCK cells (Fig. 3B) was determined by colorimetric assay ...
... Glutamine synthetase activity during cell growth Changes in GS activity have been associated with the concentration of glutamine in the medium and these were investigated. The intracellular glutamine synthetase activity of McCoy (Fig. 3A) and MDCK cells (Fig. 3B) was determined by colorimetric assay ...
Early Cell Cycle Entry and Cell Division Murine Neonatal
... showed more rapid division in vivo, upon transfer to lymphopenic RAG2⫺/⫺ hosts (Fig. 2, right column). Together, these results indicate that faster kinetics of proliferation is a universal phenomenon among neonatal T cell populations—it occurs both in vivo and in vitro, it occurs in several strains ...
... showed more rapid division in vivo, upon transfer to lymphopenic RAG2⫺/⫺ hosts (Fig. 2, right column). Together, these results indicate that faster kinetics of proliferation is a universal phenomenon among neonatal T cell populations—it occurs both in vivo and in vitro, it occurs in several strains ...
Examination of the endosomal and lysosomal pathways in
... myoA or myoB, were found to have decreased rates of intracellular particle movement (Wessels et al., 1991; Titus et al., 1993), suggesting that these motors may play a role in vesicle movement. The family of Dictyostelium myosin Is, myoA-F, are homologous to their well-studied Acanthamoeba counterpa ...
... myoA or myoB, were found to have decreased rates of intracellular particle movement (Wessels et al., 1991; Titus et al., 1993), suggesting that these motors may play a role in vesicle movement. The family of Dictyostelium myosin Is, myoA-F, are homologous to their well-studied Acanthamoeba counterpa ...
synchronous pollen mitosis and the formation of the generative cell
... a few may enter it, but it is evident that the body itself is not simply a dense entanglement of microtubules, as is evident from the electron micrograph of the central region, (Fig. 20). The polar structure is seemingly a hemisphere, with a diameter of about o-6 /i, lying closely apposed to the pla ...
... a few may enter it, but it is evident that the body itself is not simply a dense entanglement of microtubules, as is evident from the electron micrograph of the central region, (Fig. 20). The polar structure is seemingly a hemisphere, with a diameter of about o-6 /i, lying closely apposed to the pla ...
Homeotic regulation of segment-specific
... resume proliferation as postembryonic NBs in the larval stages, 47 NBs are detected in each thoracic, about 12 in the two anterior abdominal neuromeres, but only six in central abdominal segments. Furthermore, postembryonic NBs in the thorax and anterior abdomen produce hundreds of daughter cells ea ...
... resume proliferation as postembryonic NBs in the larval stages, 47 NBs are detected in each thoracic, about 12 in the two anterior abdominal neuromeres, but only six in central abdominal segments. Furthermore, postembryonic NBs in the thorax and anterior abdomen produce hundreds of daughter cells ea ...
Voltage-dependent Ionic Conductances of Type I Spiral Ganglion
... voltage clamped (Fig. 9A). Despite fine voltage control, Na current densities show great variability. In addition, Figure 9B illustrates that voltage-dependent inactivation curves (hi,,) are similar for cells possessing large and small Na current densities. It is likely that current density differen ...
... voltage clamped (Fig. 9A). Despite fine voltage control, Na current densities show great variability. In addition, Figure 9B illustrates that voltage-dependent inactivation curves (hi,,) are similar for cells possessing large and small Na current densities. It is likely that current density differen ...
bacteriophage - Micro-Rao
... life cycle. Soon after the nucleic acid is injected, the phage cycle is said to be in eclipse period. During the eclipse phase, no infectious phage particles can be found either inside or outside the bacterial cell. Eclipse phase represents the interval between the entry of phage nucleic acid into b ...
... life cycle. Soon after the nucleic acid is injected, the phage cycle is said to be in eclipse period. During the eclipse phase, no infectious phage particles can be found either inside or outside the bacterial cell. Eclipse phase represents the interval between the entry of phage nucleic acid into b ...
Poster
... occur with anxiety and substance abuse. Tetrahydrocannabinol (THC), a compound in marijuana, is a cannabinoid chemical that binds to and activates cannabinoid receptors (CB1) in the pre-synaptic cell membrane as part of neuron-to-neuron transmission in the endocannabinoid system (ECS). Glutamate in ...
... occur with anxiety and substance abuse. Tetrahydrocannabinol (THC), a compound in marijuana, is a cannabinoid chemical that binds to and activates cannabinoid receptors (CB1) in the pre-synaptic cell membrane as part of neuron-to-neuron transmission in the endocannabinoid system (ECS). Glutamate in ...
Cryoelectron Tomography: Implications for Actin Cytoskeleton
... Disclosing undistorted spatial organization of the actin cytoskeleton with molecular resolution is fundamental for understanding the cellular ultrastructure. Whereas important insights into the architecture of microfilament networks have been gained by negative staining, critical point drying, and f ...
... Disclosing undistorted spatial organization of the actin cytoskeleton with molecular resolution is fundamental for understanding the cellular ultrastructure. Whereas important insights into the architecture of microfilament networks have been gained by negative staining, critical point drying, and f ...
Batesian Mimicry: Can a Leopard Change Its Spots — and Get
... something quite different: an earlier study showed that the best mimics were found near the edge of the area of sympatry, where coral snakes are least abundant [4]; mimicry then breaks down gradually in allopatry [6]. The shape of mimicry decline thus suggests selection for mimicry is far stronger t ...
... something quite different: an earlier study showed that the best mimics were found near the edge of the area of sympatry, where coral snakes are least abundant [4]; mimicry then breaks down gradually in allopatry [6]. The shape of mimicry decline thus suggests selection for mimicry is far stronger t ...
cop2007 - Purdue Agriculture
... regulon includes substantially fewer cold-responsive genes than the CBF regulon. These results support a major role for CBFs in configuring the low temperature transcriptome in Arabidopsis. It is interesting to note that the ectopic expression of CBF genes activates the expression of several other c ...
... regulon includes substantially fewer cold-responsive genes than the CBF regulon. These results support a major role for CBFs in configuring the low temperature transcriptome in Arabidopsis. It is interesting to note that the ectopic expression of CBF genes activates the expression of several other c ...
Shield formation at the onset of zebrafish gastrulation
... ring margin contains distinct populations of cells that express genes associated with ectodermal, mesodermal and endodermal cell identities (Gritsman et al., 2000). Irrespective of their actual fate, cells within the germ ring that have internalized are named ‘hypoblast cells’, whereas noninternaliz ...
... ring margin contains distinct populations of cells that express genes associated with ectodermal, mesodermal and endodermal cell identities (Gritsman et al., 2000). Irrespective of their actual fate, cells within the germ ring that have internalized are named ‘hypoblast cells’, whereas noninternaliz ...
Shield formation at the onset of zebrafish gastrulation - MPI
... ring margin contains distinct populations of cells that express genes associated with ectodermal, mesodermal and endodermal cell identities (Gritsman et al., 2000). Irrespective of their actual fate, cells within the germ ring that have internalized are named ‘hypoblast cells’, whereas noninternaliz ...
... ring margin contains distinct populations of cells that express genes associated with ectodermal, mesodermal and endodermal cell identities (Gritsman et al., 2000). Irrespective of their actual fate, cells within the germ ring that have internalized are named ‘hypoblast cells’, whereas noninternaliz ...
Structure and Function of the F Factor and Mechanism of Conjugation
... The nucleotide sequence of the entire 33.3-kb F-tra region has revealed its genetic structure at maximal resolution (GenBank accession number U01159) (103). Figure 3 shows the organization and functional classification of the 36 open reading frames (ORFs) known or deemed likely to encode products. W ...
... The nucleotide sequence of the entire 33.3-kb F-tra region has revealed its genetic structure at maximal resolution (GenBank accession number U01159) (103). Figure 3 shows the organization and functional classification of the 36 open reading frames (ORFs) known or deemed likely to encode products. W ...
Cell Structure and Function Chapter 4 Outline Cell Theory Cell Size
... Contain membrane-bounded nucleus. Contain specialized organelles. Contain plasma membrane. ...
... Contain membrane-bounded nucleus. Contain specialized organelles. Contain plasma membrane. ...
Satisfying the Immense Energy Demands of the Body, and the
... 2004). While protons flow through ATP6, they drive the rotation of the c-rings and consequently rotate the common shaft linking F1 and F0 (Elston et al., 1998; Noji and Yoshida, 2001). This rotational energy is used by the subunit in F1 to synthesize ATP from ADP (Noji and Yoshida, 2001; Alberts e ...
... 2004). While protons flow through ATP6, they drive the rotation of the c-rings and consequently rotate the common shaft linking F1 and F0 (Elston et al., 1998; Noji and Yoshida, 2001). This rotational energy is used by the subunit in F1 to synthesize ATP from ADP (Noji and Yoshida, 2001; Alberts e ...
Roles for Regulator of G Protein Signaling Proteins in Synaptic
... Hollinger and Hepler, 2002; Willars, 2006). RGS proteins are a structurally diverse family of signaling proteins with many identified signaling partners distinct from Ga and GPCRs. In this regard, considerable evidence shows that many RGS proteins have cell signaling roles in addition to their share ...
... Hollinger and Hepler, 2002; Willars, 2006). RGS proteins are a structurally diverse family of signaling proteins with many identified signaling partners distinct from Ga and GPCRs. In this regard, considerable evidence shows that many RGS proteins have cell signaling roles in addition to their share ...
New Insights on Plant Cell Elongation: A Role for Acetylcholine
... medium (data not shown); maximum elongation after 16 h was observed in hypocotyl segments treated with auxin alone. Our observation differs from Lawson and colleagues [38], who reported that higher concentration of ACh had an inhibitory effect on elongation. In any case, the literature does not prov ...
... medium (data not shown); maximum elongation after 16 h was observed in hypocotyl segments treated with auxin alone. Our observation differs from Lawson and colleagues [38], who reported that higher concentration of ACh had an inhibitory effect on elongation. In any case, the literature does not prov ...
necessary amino acids and vitamins
... The growth rate in the glutamine + cysteine medium was slightly less than that in Eagle's medium, but the in vitro life time was similar. Folic acid was the only vitamin needed to support cell growth in vitro. The addition of other vitamins had no stimulatory effect with the possible exception of ni ...
... The growth rate in the glutamine + cysteine medium was slightly less than that in Eagle's medium, but the in vitro life time was similar. Folic acid was the only vitamin needed to support cell growth in vitro. The addition of other vitamins had no stimulatory effect with the possible exception of ni ...
BioWire_Progress_Report,_Week_One
... Changing the strength of the RBS (weaker, weak, medium, strong) Altering repressor/operator affinity through mutation in the operator region Mutating promoter sequences Increasing AHL degradation rate through raising the pH of the medium Lowering plasmid copy number through mutations in replication ...
... Changing the strength of the RBS (weaker, weak, medium, strong) Altering repressor/operator affinity through mutation in the operator region Mutating promoter sequences Increasing AHL degradation rate through raising the pH of the medium Lowering plasmid copy number through mutations in replication ...
BYSTANDER HELP IN PRIMARY IMMUNE RESPONSES IN
... which secondary, hapten-specific antibody responses require that hapten be coupled to the same carrier used for initial priming (7-10) . On the other hand, in bystander help, Th cells primed to a particular carrier in the presence of that carrier support secondary antibody responses to hapten linked ...
... which secondary, hapten-specific antibody responses require that hapten be coupled to the same carrier used for initial priming (7-10) . On the other hand, in bystander help, Th cells primed to a particular carrier in the presence of that carrier support secondary antibody responses to hapten linked ...
Protein actors sustaining arbuscular mycorrhizal symbiosis
... generated an increasing depository of plant and fungal proteins eligible for sustaining AM accommodation and functioning, a bottleneck exists for their functional analysis as these experiments are difficult to carry out with mycorrhiza. Nonetheless, the expansion of gene-tophenotype reverse genetic ...
... generated an increasing depository of plant and fungal proteins eligible for sustaining AM accommodation and functioning, a bottleneck exists for their functional analysis as these experiments are difficult to carry out with mycorrhiza. Nonetheless, the expansion of gene-tophenotype reverse genetic ...
BMI - Network Protocols Lab
... neurotransmitter in the context of depressive disorders. Literature Review ...
... neurotransmitter in the context of depressive disorders. Literature Review ...
Poster
... more than one phosphate group, to tau, an intracellular microtubule protein. Thrombin is a serine protease involved in the final step of blood coagulation and is normally present in the brain’s neurons, where it cleaves tau. It is necessary for thrombin to cleave tau in order to prevent clumping of ...
... more than one phosphate group, to tau, an intracellular microtubule protein. Thrombin is a serine protease involved in the final step of blood coagulation and is normally present in the brain’s neurons, where it cleaves tau. It is necessary for thrombin to cleave tau in order to prevent clumping of ...