Shaping the metaphase chromosome: coordination of cohesion and
... The cohesin complex purified from HeLa cells can bind to double-stranded DNA and induce the formation of large protein±DNA aggregates in vitro.(10) In the presence of topoisomerase II, cohesin stimulates intermolecular catenation of circular DNA molecules. This activity is in striking contrast to th ...
... The cohesin complex purified from HeLa cells can bind to double-stranded DNA and induce the formation of large protein±DNA aggregates in vitro.(10) In the presence of topoisomerase II, cohesin stimulates intermolecular catenation of circular DNA molecules. This activity is in striking contrast to th ...
RNAi (PDF) (1.14 MB)
... RNA interference (RNAi) has emerged as one of the most promising tools for biological research. It is thought that RNAi may have evolved originally as a defense mechanism against foreign parasitic nucleic acid sequences and functions by specifically knocking down the activity of target genes. RNAi ha ...
... RNA interference (RNAi) has emerged as one of the most promising tools for biological research. It is thought that RNAi may have evolved originally as a defense mechanism against foreign parasitic nucleic acid sequences and functions by specifically knocking down the activity of target genes. RNAi ha ...
Pdf - Text of NPTEL IIT Video Lectures
... Now, if we see the entire bacterial world based on its shape, the bacteria can be categorized. And, if we see its shape, you will find that, some of this bacteria are round or spherical in nature, some are rod and some are spiral in nature. Now, this are the, this, this are some of this classificat ...
... Now, if we see the entire bacterial world based on its shape, the bacteria can be categorized. And, if we see its shape, you will find that, some of this bacteria are round or spherical in nature, some are rod and some are spiral in nature. Now, this are the, this, this are some of this classificat ...
Somite-derived cells replace ventral aortic
... and provide aortic smooth muscle cells of the trunk Claire Pouget1, Rodolphe Gautier1, Marie-Aimee Teillet1,2 and Thierry Jaffredo1,* We have previously shown that endothelial cells of the aortic floor give rise to hematopoietic cells, revealing the existence of an aortic hemangioblast. It has been ...
... and provide aortic smooth muscle cells of the trunk Claire Pouget1, Rodolphe Gautier1, Marie-Aimee Teillet1,2 and Thierry Jaffredo1,* We have previously shown that endothelial cells of the aortic floor give rise to hematopoietic cells, revealing the existence of an aortic hemangioblast. It has been ...
Isolation and purification of cell wall polysaccharide of Bacillus
... using gas liquid chromatography (GLC) and 13CN M R spectroscopy. Samples for GLC were prepared by suspending 1 mg of polysaccharide in 250 pA 2M trifluoroacetic acid and heated at 120°C for 1 h. The samples were cooled and acid removed under a flow of air and by co-distillation with isopropyl alcoho ...
... using gas liquid chromatography (GLC) and 13CN M R spectroscopy. Samples for GLC were prepared by suspending 1 mg of polysaccharide in 250 pA 2M trifluoroacetic acid and heated at 120°C for 1 h. The samples were cooled and acid removed under a flow of air and by co-distillation with isopropyl alcoho ...
the Cytoskeleton in Plant Development1
... stimuli (refer to section III for details). The ensuing mitosis is heralded by a displacement of the nucleus to the cell centre, i.e., to the site where the prospective cell plate will be formed. Simultaneously, radial microtubules emanate from the nuclear surface and merge with the cortical cytoske ...
... stimuli (refer to section III for details). The ensuing mitosis is heralded by a displacement of the nucleus to the cell centre, i.e., to the site where the prospective cell plate will be formed. Simultaneously, radial microtubules emanate from the nuclear surface and merge with the cortical cytoske ...
IN VITRO BARK EXTRACTS
... is a Gram-positive cocci and Escherichia coli is a Gram-negative enterobacteria. The findings of this study showed that Cinnamomum zeylanicum bark extracts had inhibited both Gram-positive bacteria and Gram-negative bacteria indicating broad spectrum inhibitory effect. Gram positive bacteria were mo ...
... is a Gram-positive cocci and Escherichia coli is a Gram-negative enterobacteria. The findings of this study showed that Cinnamomum zeylanicum bark extracts had inhibited both Gram-positive bacteria and Gram-negative bacteria indicating broad spectrum inhibitory effect. Gram positive bacteria were mo ...
Simplified protocols for the preparation of genomic DNA
... by chelating agents, which makes them applicable in combination with EDTA. The application of achromopeptidase has been limited to the disruption of Grampositive cells, principally staphylococci [9], although applications with other bacteria have been reported. Cell disruption by detergent treatmen ...
... by chelating agents, which makes them applicable in combination with EDTA. The application of achromopeptidase has been limited to the disruption of Grampositive cells, principally staphylococci [9], although applications with other bacteria have been reported. Cell disruption by detergent treatmen ...
Eubacterial sigma
... that interact with the 310 region and residues involved in promoter melting. The c70 -protein family can be divided into three di¡erent functionally and structurally related groups [18] (Fig. 3, Table 1). Group 1 comprises the primary c-factors responsible for the transcription of most genes express ...
... that interact with the 310 region and residues involved in promoter melting. The c70 -protein family can be divided into three di¡erent functionally and structurally related groups [18] (Fig. 3, Table 1). Group 1 comprises the primary c-factors responsible for the transcription of most genes express ...
Methods for distinguishing between protein-coding and
... Introduction – the gray area between coding and noncoding transcripts Genome-wide surveys of transcription in mammalians [1-8] and more recently in other vertebrates [9-13] have shown that vertebrate genomes are pervasively transcribed and produce a large variety of processed transcripts, many of wh ...
... Introduction – the gray area between coding and noncoding transcripts Genome-wide surveys of transcription in mammalians [1-8] and more recently in other vertebrates [9-13] have shown that vertebrate genomes are pervasively transcribed and produce a large variety of processed transcripts, many of wh ...
REVIEW ARTICLE Mechanisms of pattern formation in development
... Division of an heterogeneous egg: different parts of the egg bind different molecules (indicated by different shading) resulting in different blastomere cells. Asymmetric mitosis: molecules are differentially transported into different parts of a cell resulting in different daughter cells. Internal ...
... Division of an heterogeneous egg: different parts of the egg bind different molecules (indicated by different shading) resulting in different blastomere cells. Asymmetric mitosis: molecules are differentially transported into different parts of a cell resulting in different daughter cells. Internal ...
glycolytic and associated enzymes of rainbow trout (oncorhynchus
... anaerobic oxidation of glucose to lactate. Red cell pyruvate kinase (PK) was inhibited by ATP (/50 =5mmoll~ 1 ), but was not sensitive to alanine inhibition or fructose-1,6-bisphosphate activation. The properties of red cell PK were similar to those of the muscle-type enzyme. Lactate dehydrogenase ( ...
... anaerobic oxidation of glucose to lactate. Red cell pyruvate kinase (PK) was inhibited by ATP (/50 =5mmoll~ 1 ), but was not sensitive to alanine inhibition or fructose-1,6-bisphosphate activation. The properties of red cell PK were similar to those of the muscle-type enzyme. Lactate dehydrogenase ( ...
Microbial Cell Structure and Function
... – some (type IV fimbriae) required for twitching motility or gliding motility that occurs in some bacteria ...
... – some (type IV fimbriae) required for twitching motility or gliding motility that occurs in some bacteria ...
Cnidarians: An evolutionarily conserved model system for
... 1984). An important consequence is that the passively displaced cells have to assess their relative position in the organism continuously. Hence, patterning systems necessary to provide this information are continuously active in Hydra polyps. By contrast, in mammals, most of these morphogenetic sig ...
... 1984). An important consequence is that the passively displaced cells have to assess their relative position in the organism continuously. Hence, patterning systems necessary to provide this information are continuously active in Hydra polyps. By contrast, in mammals, most of these morphogenetic sig ...
Formation Costimulation in Immunological Synapse Live
... terminus, allowing the peptide to load efficiently into the peptidebinding groove (29, 30). With this system, we can follow the movement of specific MHC:peptide complexes upon T cell-APC interaction by video microscopy. Because all specific moth cytochrome c peptide (MCC)-loaded MHC molecules and on ...
... terminus, allowing the peptide to load efficiently into the peptidebinding groove (29, 30). With this system, we can follow the movement of specific MHC:peptide complexes upon T cell-APC interaction by video microscopy. Because all specific moth cytochrome c peptide (MCC)-loaded MHC molecules and on ...
A Cytochemical Study of the Stem Cell Concept in Specimens o£ a
... and that multipolar figures are of secondary origin." The number of atypical mitoses in tissues may vary from the infrequent occurrence of somatic aneuploidy and polyploidy in some normal (5) and tumor tissues to the more extensive occurrence of such types of aberrancy in mammalian liver, many tumor ...
... and that multipolar figures are of secondary origin." The number of atypical mitoses in tissues may vary from the infrequent occurrence of somatic aneuploidy and polyploidy in some normal (5) and tumor tissues to the more extensive occurrence of such types of aberrancy in mammalian liver, many tumor ...
Transmembrane Domain (TM) 9 Represents a Novel
... represent two different regions within Pgp1 the amino sequences of which, when altered, affect cross-resistance patterns in similar ways. When the two mutant regions are expressed together in a chimeric protein, the level of ActD resistance, relative to DC-3F, is 10-fold higher than that observed fr ...
... represent two different regions within Pgp1 the amino sequences of which, when altered, affect cross-resistance patterns in similar ways. When the two mutant regions are expressed together in a chimeric protein, the level of ActD resistance, relative to DC-3F, is 10-fold higher than that observed fr ...
Full-Text PDF
... to five simultaneous gene deletions identified by the SL finder for Yeast 7.11. Figure 1 pictorially classifies the agreement of in silico lethality information with in vivo gene deletion information (see Supplemental File S4: Table 2). The vertical axis refers to in silico predictions while the hor ...
... to five simultaneous gene deletions identified by the SL finder for Yeast 7.11. Figure 1 pictorially classifies the agreement of in silico lethality information with in vivo gene deletion information (see Supplemental File S4: Table 2). The vertical axis refers to in silico predictions while the hor ...
Structure and function of steroid receptor AF1 transactivation domains
... autonomous function of this domain required the PR-DBD, suggesting intra-domain communication [57]. A second transactivation domain, termed AF3, is present in the PR-B isoform, and consists of the first 165 amino acids [59] (Figure 1). Comparison of the PR sequences from mammalian species and chicke ...
... autonomous function of this domain required the PR-DBD, suggesting intra-domain communication [57]. A second transactivation domain, termed AF3, is present in the PR-B isoform, and consists of the first 165 amino acids [59] (Figure 1). Comparison of the PR sequences from mammalian species and chicke ...
Embodied Computation Applying the Physics of Computation to Artificial Morphogenesis
... intelligence and cognitive science to suppose that intelligence requires complex internal models of the body and the world in order to behave competently, it has been found that embodied agents can function competently without these complex models by exploiting their bodies and their physical enviro ...
... intelligence and cognitive science to suppose that intelligence requires complex internal models of the body and the world in order to behave competently, it has been found that embodied agents can function competently without these complex models by exploiting their bodies and their physical enviro ...
Ultrastructure of diaphragm from dystrophic α-sarcoglycan
... (DAG) complex which forms a mechanical link between the intracellular cytoskeleton and extracellular matrix. The DAG complex function is to protect the muscle membrane from the stress of contractile activity and as a structure for the docking of signalling proteins. Genetic defects of DAG components ...
... (DAG) complex which forms a mechanical link between the intracellular cytoskeleton and extracellular matrix. The DAG complex function is to protect the muscle membrane from the stress of contractile activity and as a structure for the docking of signalling proteins. Genetic defects of DAG components ...
progress and problems with the use of viral vectors for
... In April 2000, a paper was published in the journal Science that marked the highest point in the turbulent history of gene therapy. In a paper entitled “Gene therapy of human severe combined immunodeficiency (SCID)-XI disease”, Maria Cavazzana-Calvo, Alain Fischer and colleagues at the Necker Hospit ...
... In April 2000, a paper was published in the journal Science that marked the highest point in the turbulent history of gene therapy. In a paper entitled “Gene therapy of human severe combined immunodeficiency (SCID)-XI disease”, Maria Cavazzana-Calvo, Alain Fischer and colleagues at the Necker Hospit ...
Scrape Procedure
... others, the parasites are situated within a clearly defined parasitophorous vacuole (PV) or lay in direct contact with the PV membrane within the new host cell. The earliest developmental stage seen are the meronts which are irregular in outline and bounded by a plasma membrane, and occur randomly i ...
... others, the parasites are situated within a clearly defined parasitophorous vacuole (PV) or lay in direct contact with the PV membrane within the new host cell. The earliest developmental stage seen are the meronts which are irregular in outline and bounded by a plasma membrane, and occur randomly i ...