Calcium oscillations in higher plants Nicola H Evans*, Martin R
... 15. Pei ZM, Murata Y, Benning G, Phomine S, Klusener B, Allen GJ, Grill E, Schroeder JI: Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells. Nature ...
... 15. Pei ZM, Murata Y, Benning G, Phomine S, Klusener B, Allen GJ, Grill E, Schroeder JI: Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells. Nature ...
The Plant Membrane-Associated REMORIN1.3 Accumulates in
... underlying the biogenesis of the EHM are unknown. Remarkably, several plasma membrane-localized proteins are excluded from the EHM, but the remorin REM1.3 accumulates around P. infestans haustoria. Here, we used overexpression, colocalization with reporter proteins, and superresolution microscopy in ...
... underlying the biogenesis of the EHM are unknown. Remarkably, several plasma membrane-localized proteins are excluded from the EHM, but the remorin REM1.3 accumulates around P. infestans haustoria. Here, we used overexpression, colocalization with reporter proteins, and superresolution microscopy in ...
FGF signaling is essential for the early events in the development of
... cultured embryos with exogenous, human recombinant FGF led to abnormalities in neural induction and development, notochord formation and somitogenesis as studied by gross morphology and histology. Overall growth and development was also adversely affected as seen from the measurement of body axis le ...
... cultured embryos with exogenous, human recombinant FGF led to abnormalities in neural induction and development, notochord formation and somitogenesis as studied by gross morphology and histology. Overall growth and development was also adversely affected as seen from the measurement of body axis le ...
Lignin biosynthesis in Norway spruce: from a model system to the tree
... We used, as a model, a Norway spruce tissue culture line that produces extracellular lignin into the culture medium, and showed that lignin polymerisation in the tissue culture depends on peroxidase activity. We identified in the culture medium a significant NADH oxidase activity that could generate ...
... We used, as a model, a Norway spruce tissue culture line that produces extracellular lignin into the culture medium, and showed that lignin polymerisation in the tissue culture depends on peroxidase activity. We identified in the culture medium a significant NADH oxidase activity that could generate ...
RIBOSOMES, PEPTIDES AND ANTIBIOTIC RESISTANCE
... ORF. Such stalling occurs at codon 6 of the cat leader ORF and at codon 9 of the leader ORFs of cmlA and erm genes. Curiously, ribosome stalling depends on the amino acid sequence of the translated short peptide; missense mutations in the leader ORF changing the nature of the encoded amino acids red ...
... ORF. Such stalling occurs at codon 6 of the cat leader ORF and at codon 9 of the leader ORFs of cmlA and erm genes. Curiously, ribosome stalling depends on the amino acid sequence of the translated short peptide; missense mutations in the leader ORF changing the nature of the encoded amino acids red ...
Compare TIRF Geometries
... TIRF is a powerful analytical technique with numerous applications in the area of life sciences [1-3]. In particular, TIRF is “...a method uniquely suited to image the plasma membrane with its associated organelles and macromolecules in living cells. The method shows even the smallest vesicles made ...
... TIRF is a powerful analytical technique with numerous applications in the area of life sciences [1-3]. In particular, TIRF is “...a method uniquely suited to image the plasma membrane with its associated organelles and macromolecules in living cells. The method shows even the smallest vesicles made ...
Downloaded - Stem-cell and Brain Research Institute
... for bmal1 and clock genes in regulating glucose homeostasis (24) and lipid metabolism (25–28). Moreover, the cross talk between ROR␣ and Rev-erb␣ was shown to be physiologically important for the control of cholesterol and triglyceride metabolism (29–31). In turn, the peripheral clocks can be coordi ...
... for bmal1 and clock genes in regulating glucose homeostasis (24) and lipid metabolism (25–28). Moreover, the cross talk between ROR␣ and Rev-erb␣ was shown to be physiologically important for the control of cholesterol and triglyceride metabolism (29–31). In turn, the peripheral clocks can be coordi ...
The Role Of The Planar Cell Polarity Pathway In The Second Heart
... allowed us to put forth a novel mechanism to understand how SHF progenitors may be uni-directionally and cohesively deployed in a PCP-dependent fashion to elongate the ...
... allowed us to put forth a novel mechanism to understand how SHF progenitors may be uni-directionally and cohesively deployed in a PCP-dependent fashion to elongate the ...
The amoebae plate test implicates a paralogue of lpxB in the
... Most of the genes of the icm/dot loci are predicted to encode membrane-spanning proteins (Segal & Shuman, 1999a). Interestingly, the IcmG protein contains a t-SNARE domain, and thus this membrane protein might play a direct role in altering host cell vesicle trafficking (Morozova et al., 2004). Bioc ...
... Most of the genes of the icm/dot loci are predicted to encode membrane-spanning proteins (Segal & Shuman, 1999a). Interestingly, the IcmG protein contains a t-SNARE domain, and thus this membrane protein might play a direct role in altering host cell vesicle trafficking (Morozova et al., 2004). Bioc ...
Arabidopsis HAP2 (GCS1) is a sperm-specific gene
... half of the ovules, as expected (116/234, 50%, Fig. 1B,C). By contrast, when hap2-1/HAP2 anthers were used, a smaller proportion of GUS+ pollen tubes entered a micropyle and burst (115/505, 23%; Fig. 1B,D). Furthermore, hap2-1 pollen tubes were observed growing on portions of the ovule where wild-ty ...
... half of the ovules, as expected (116/234, 50%, Fig. 1B,C). By contrast, when hap2-1/HAP2 anthers were used, a smaller proportion of GUS+ pollen tubes entered a micropyle and burst (115/505, 23%; Fig. 1B,D). Furthermore, hap2-1 pollen tubes were observed growing on portions of the ovule where wild-ty ...
Binding properties of YjeQ (RsgA), RbfA, RimM and Era to assembly
... dead-end products of assembly and still progress into mature 30S subunits that assemble into 70S ribosomes, however, maturation occurs at a much slower pace in the absence of YjeQ or RimM. Quantitative mass spectrometry analysis (qMS) revealed these immature 30S subunits when purified under non-diss ...
... dead-end products of assembly and still progress into mature 30S subunits that assemble into 70S ribosomes, however, maturation occurs at a much slower pace in the absence of YjeQ or RimM. Quantitative mass spectrometry analysis (qMS) revealed these immature 30S subunits when purified under non-diss ...
1 Calcium at the Cell Wall
... (1967), applying autoradiography to pollen tubes that had been administered with 45Ca2+, showed extensive labeling of the tube apex, which he interpreted as Ca2+ binding by the acidic pectic residues. A similar study by Jaffe et al. (1975) on lily pollen tubes yielded similar results, but the author ...
... (1967), applying autoradiography to pollen tubes that had been administered with 45Ca2+, showed extensive labeling of the tube apex, which he interpreted as Ca2+ binding by the acidic pectic residues. A similar study by Jaffe et al. (1975) on lily pollen tubes yielded similar results, but the author ...
Can fast-growing plantation trees escape biochemical down
... is no increase in carbon retention by the trees under elevated [CO2] (Körner et al. 2005). If mature natural forests are unable to accumulate more C, then this leaves plantation forests as the only option for the partial mitigation of rising [CO2] by woody systems (Eamus & Jarvis 1989). Fastgrowing ...
... is no increase in carbon retention by the trees under elevated [CO2] (Körner et al. 2005). If mature natural forests are unable to accumulate more C, then this leaves plantation forests as the only option for the partial mitigation of rising [CO2] by woody systems (Eamus & Jarvis 1989). Fastgrowing ...
The actin cytoskeleton is a target of the self
... for the signals triggered by the SI response is discussed. It is suggested that these F-actin alterations may be Ca2+-mediated and that this could be a mechanism whereby SI-induced tip growth inhibition is achieved. The potential for actin-binding proteins to act as key mediators of this response is ...
... for the signals triggered by the SI response is discussed. It is suggested that these F-actin alterations may be Ca2+-mediated and that this could be a mechanism whereby SI-induced tip growth inhibition is achieved. The potential for actin-binding proteins to act as key mediators of this response is ...
Microtubule Independent Vesiculation of Golgi Membranes and the
... of cdc2 kinase that alter the dynamics of the Golgi membranes have yet to be identified. We have recently reported the identification of a novel nat- ...
... of cdc2 kinase that alter the dynamics of the Golgi membranes have yet to be identified. We have recently reported the identification of a novel nat- ...
Arabidopsis DET1 degrades HFR1 but stabilizes PIF1 to precisely
... phenotypes (Fig. S2 and Fig. 1A). In addition, overexpression of COP10 did not germinate even under the red light (R) condition (with an additional 5 min of red light irradiation before dark incubation) (Fig. S2), indicating the crucial roles of the CDD complex in regulating seed germination. Then w ...
... phenotypes (Fig. S2 and Fig. 1A). In addition, overexpression of COP10 did not germinate even under the red light (R) condition (with an additional 5 min of red light irradiation before dark incubation) (Fig. S2), indicating the crucial roles of the CDD complex in regulating seed germination. Then w ...
Activin-mediated mesoderm induction requires FGF
... activated by tyrosine kinase receptors, has been reported to eliminate only the FGF signaling pathway (MacNicol et al., 1993). Similarly, FGF can activate MAP kinase, a key regulator in the intracellular signal transduction process, whereas activin cannot (Graves, 1994; LaBonne and Whitman, 1994). W ...
... activated by tyrosine kinase receptors, has been reported to eliminate only the FGF signaling pathway (MacNicol et al., 1993). Similarly, FGF can activate MAP kinase, a key regulator in the intracellular signal transduction process, whereas activin cannot (Graves, 1994; LaBonne and Whitman, 1994). W ...
PDF
... To generate clones of cells mutant for tup, y, w, hs-FLP1.22; tup, FRT40A/CyO males were crossed with either y, w, hs-FLP1.22; ubi-GFP, FRT40A/CyO or y, w, hs-FLP1.22; P{y+}25F, ck13, FRT40A/CyO or f, hsFLP1.22; P{f+}30, ck, FRT40A/CyO females. Homozygous tup clones were induced at different develop ...
... To generate clones of cells mutant for tup, y, w, hs-FLP1.22; tup, FRT40A/CyO males were crossed with either y, w, hs-FLP1.22; ubi-GFP, FRT40A/CyO or y, w, hs-FLP1.22; P{y+}25F, ck13, FRT40A/CyO or f, hsFLP1.22; P{f+}30, ck, FRT40A/CyO females. Homozygous tup clones were induced at different develop ...
The Calpain-Calpastatin System and the Calcium Paradox in the
... enzyme is a millimolar isoform (m-calpain). As shown in Table 1, at 5 mM, only Mn2+ and Sr2+, activate calpain, substituting for Ca 2+, which activates calpain to a maximum extent. Furthermore, in the presence of 1 mM Ca2+, Mn2+ and Sr2+, show a synergistic effect (Table 1). Among the other cations ...
... enzyme is a millimolar isoform (m-calpain). As shown in Table 1, at 5 mM, only Mn2+ and Sr2+, activate calpain, substituting for Ca 2+, which activates calpain to a maximum extent. Furthermore, in the presence of 1 mM Ca2+, Mn2+ and Sr2+, show a synergistic effect (Table 1). Among the other cations ...
Mcm10: A Dynamic Scaffold at Eukaryotic Replication Forks
... The NTD is common among Mcm10 proteins from yeast to humans, but is not essential and less well conserved than the central ID (Figures 1 and 3) [74,75]. Functionally, the NTD contributes to self-oligomerization and partner protein interaction [39,50]. Homocomplex formation of Xenopus and human Mcm10 ...
... The NTD is common among Mcm10 proteins from yeast to humans, but is not essential and less well conserved than the central ID (Figures 1 and 3) [74,75]. Functionally, the NTD contributes to self-oligomerization and partner protein interaction [39,50]. Homocomplex formation of Xenopus and human Mcm10 ...
Evaluating rRNA as an indicator of microbial activity in
... cell growth rate consistently lagged behind rRNA concentration or no discernible relationship. The latter was observed in Vibrio fischeri, and included periods during which growth rate decreased while rRNA content increased. Again, since growth activity likely accounts for much of total activity, th ...
... cell growth rate consistently lagged behind rRNA concentration or no discernible relationship. The latter was observed in Vibrio fischeri, and included periods during which growth rate decreased while rRNA content increased. Again, since growth activity likely accounts for much of total activity, th ...
Par3/Baz levels control epithelial folding at actomyosin
... PSBs) first enrich actomyosin at gastrulation, during germ-band extension (Tetley et al., 2016). Wingless signaling on one side of the compartmental boundary maintains these enrichments at germband extended stage, where they act as mechanical barriers to keep dividing cells in their compartment of o ...
... PSBs) first enrich actomyosin at gastrulation, during germ-band extension (Tetley et al., 2016). Wingless signaling on one side of the compartmental boundary maintains these enrichments at germband extended stage, where they act as mechanical barriers to keep dividing cells in their compartment of o ...
The yolk syncytial layer regulates myocardial
... 1 (Sigma) at 1:200. For whole-mount anti-Laminin 1 staining, we used the ABC staining kit (Vector Laboratory) after treating with an anti-rabbit IgG biotin-conjugated antibody. Fluorescence confocal images were acquired using a Zeiss LSM5 Pascal confocal microscope. ...
... 1 (Sigma) at 1:200. For whole-mount anti-Laminin 1 staining, we used the ABC staining kit (Vector Laboratory) after treating with an anti-rabbit IgG biotin-conjugated antibody. Fluorescence confocal images were acquired using a Zeiss LSM5 Pascal confocal microscope. ...
Biology I End-of-Course
... A. The procedure for DNA fingerprinting is an accurate series of simple techniques. This answer is not correct. How simple or complex the technique is does not indicate whether this procedure is a reliable way to identify individuals. B. The probability of two people having similar DNA fingerprints ...
... A. The procedure for DNA fingerprinting is an accurate series of simple techniques. This answer is not correct. How simple or complex the technique is does not indicate whether this procedure is a reliable way to identify individuals. B. The probability of two people having similar DNA fingerprints ...