Induction of stable ER–plasma-membrane junctions by Kv2.1
... <20 nm gap between the two membranes (Carrasco and Meyer, 2011; Orci et al., 2009). Thus, in order to examine the spatial relationship between Kv2.1 clusters on the plasma membrane and the underlying cER at high resolution, we used immuno-electron microscopy. HEK cells were either mock transfected ( ...
... <20 nm gap between the two membranes (Carrasco and Meyer, 2011; Orci et al., 2009). Thus, in order to examine the spatial relationship between Kv2.1 clusters on the plasma membrane and the underlying cER at high resolution, we used immuno-electron microscopy. HEK cells were either mock transfected ( ...
Dissection of molecular interactions of replication protein A in
... of RPA only modestly affected the affinity of RPA but these aromatic residue mutants were unable to support DNA repair while functioning in DNA replication. To understand the molecular basis of this phenotype, I have characterized the interactions of the aromatic mutants with different length ssDNAs ...
... of RPA only modestly affected the affinity of RPA but these aromatic residue mutants were unable to support DNA repair while functioning in DNA replication. To understand the molecular basis of this phenotype, I have characterized the interactions of the aromatic mutants with different length ssDNAs ...
Lateral root initiation is a probabilistic event whose frequency is set
... the initial events that lead to LRI. They also noted that the peaks of auxin response correlate with the waving pattern of growth that their plants displayed. Such waving growth depends on auxin transporters and has been hypothesized to depend on gravity (Oliva and Dunand, 2007); however, roots grow ...
... the initial events that lead to LRI. They also noted that the peaks of auxin response correlate with the waving pattern of growth that their plants displayed. Such waving growth depends on auxin transporters and has been hypothesized to depend on gravity (Oliva and Dunand, 2007); however, roots grow ...
The Presynaptic Microtubule Cytoskeleton in Physiological and
... DeLorenzo, 1981). Such phosphorylation is stimulated by activity (Burke and DeLorenzo, 1982), suggesting a functional link between neurotransmitter release and tubulin post-translational modifications. Also MTs directly bind with the SV membrane protein Synaptotagmin I, where the interaction occurs ...
... DeLorenzo, 1981). Such phosphorylation is stimulated by activity (Burke and DeLorenzo, 1982), suggesting a functional link between neurotransmitter release and tubulin post-translational modifications. Also MTs directly bind with the SV membrane protein Synaptotagmin I, where the interaction occurs ...
Sequestration of host metabolism by an intracellular pathogen | eLife
... host cell. Thus, C.trachomatis has developed ways to import these nutrients into inclusions, and, more generally, to take the control of its interactions with the host cell. The inclusions built up by C. trachomatis contain a high amount of glycogen, a carbohydrate that generally acts as an energy s ...
... host cell. Thus, C.trachomatis has developed ways to import these nutrients into inclusions, and, more generally, to take the control of its interactions with the host cell. The inclusions built up by C. trachomatis contain a high amount of glycogen, a carbohydrate that generally acts as an energy s ...
PDF
... (A-D) A series of photographs showing in vitro development of isolated 14-day palatal epithelium cultured with its basal surface against a Millipore filter. The photographs show the living culture at 0, 24, 48 and 72 h of incubation. At 0 h (Fig. A), the medial region is indicated by an anterior and ...
... (A-D) A series of photographs showing in vitro development of isolated 14-day palatal epithelium cultured with its basal surface against a Millipore filter. The photographs show the living culture at 0, 24, 48 and 72 h of incubation. At 0 h (Fig. A), the medial region is indicated by an anterior and ...
Bactericidal/Permeability-Increasing Protein Release in Whole
... BPI, at concentrations as low as 10 pg/mL. Since PMNL express two receptors for TNF-a, a 55-kDa (TNFRss ) and a 75kDa (TNFR75 ) receptor, which are both able to mediate part of TNF-a activities [27, 29], we analyzed which receptor was involved in TNF-a-induced BPI release. To this end, whole blood w ...
... BPI, at concentrations as low as 10 pg/mL. Since PMNL express two receptors for TNF-a, a 55-kDa (TNFRss ) and a 75kDa (TNFR75 ) receptor, which are both able to mediate part of TNF-a activities [27, 29], we analyzed which receptor was involved in TNF-a-induced BPI release. To this end, whole blood w ...
Effects of Episodic Turbulence on Diatoms, with Comments on the Use of Evans Blue Stain for Live-Dead Determinations
... example, NO3 uptake increased 10% in Ditylum brightwellii cultures exposed to intermediate shear levels in a Couette cylinder, but uptake of 14C-bicarbonate decreased (Pasciack and Gavis 1975). Turbulent motion is one of the mechanisms that cause cell aggregation. If turbulence induces aggregate for ...
... example, NO3 uptake increased 10% in Ditylum brightwellii cultures exposed to intermediate shear levels in a Couette cylinder, but uptake of 14C-bicarbonate decreased (Pasciack and Gavis 1975). Turbulent motion is one of the mechanisms that cause cell aggregation. If turbulence induces aggregate for ...
1 bio. hints of aipmt mt – viii
... (c) Echinoderms have calcareous endoskeleton. 26. Ans. (c) Hint: Failure of cytokinesis after telophase results in polyploidy. In sickle cell anaemia, GAG is changed to GUG. 28. Ans. (c) Hint: Human genome contains 3164.7 million nucleotide bases. 33. Ans. (a) Hint: In Meselson & Stahl experiment th ...
... (c) Echinoderms have calcareous endoskeleton. 26. Ans. (c) Hint: Failure of cytokinesis after telophase results in polyploidy. In sickle cell anaemia, GAG is changed to GUG. 28. Ans. (c) Hint: Human genome contains 3164.7 million nucleotide bases. 33. Ans. (a) Hint: In Meselson & Stahl experiment th ...
Airway surface liquid pH in well-differentiated airway - AJP-Cell
... by brief sonication) was added onto the ASL to change pH by 0.4–0.5 pH units. In some experiments, transport inhibitors were added to the perfusate or to both the perfusate and the ASL as described in RESULTS. Measurements in mouse trachea in vivo. Mice (25–35 g body wt) were anesthetized with ketam ...
... by brief sonication) was added onto the ASL to change pH by 0.4–0.5 pH units. In some experiments, transport inhibitors were added to the perfusate or to both the perfusate and the ASL as described in RESULTS. Measurements in mouse trachea in vivo. Mice (25–35 g body wt) were anesthetized with ketam ...
Regulation of reactive oxygen species homeostasis
... as by ABA and SA hormones (Lee et al., 1999; Pei et al., 2000; Khokon et al., 2011). Therefore, we tested whether H2O2 is also an important secondary messenger in CK-mediated stomatal immunity. We monitored H2O2 production in epidermal strips treated with 1 µM t-Z or 5 µM flg22 using the fluorescen ...
... as by ABA and SA hormones (Lee et al., 1999; Pei et al., 2000; Khokon et al., 2011). Therefore, we tested whether H2O2 is also an important secondary messenger in CK-mediated stomatal immunity. We monitored H2O2 production in epidermal strips treated with 1 µM t-Z or 5 µM flg22 using the fluorescen ...
Evidence for an apical Na–Cl cotransporter involved in ion uptake in
... Cation–chloride cotransporters, such as the Na+/K+/2Cl– cotransporter (NKCC) and Na+/Cl– cotransporter (NCC), are localized to the apical or basolateral plasma membranes of epithelial cells and are involved in active ion absorption or secretion. The objectives of this study were to clone and identif ...
... Cation–chloride cotransporters, such as the Na+/K+/2Cl– cotransporter (NKCC) and Na+/Cl– cotransporter (NCC), are localized to the apical or basolateral plasma membranes of epithelial cells and are involved in active ion absorption or secretion. The objectives of this study were to clone and identif ...
Ribosomal Stalk Protein L12: Structure, Function and
... factors during various steps of translation. The P-site is located next to the A-site on the ribosome. During initiation of protein synthesis, initiator tRNA (fMet-tRNAfMet) binds directly to the P-site on the 30S subunit. Other than that, peptidyl tRNAs occupy the P-site after translocation until t ...
... factors during various steps of translation. The P-site is located next to the A-site on the ribosome. During initiation of protein synthesis, initiator tRNA (fMet-tRNAfMet) binds directly to the P-site on the 30S subunit. Other than that, peptidyl tRNAs occupy the P-site after translocation until t ...
VegT initiates mesoderm induction - Development
... was stripped and reprobed for a loading control, plakoglobin. (b) Northern blot of oocytes, either uninjected (Un) or injected with 2.5 or 5 ng of the antisense oligo used in Zhang et al. (1998) (old), or the same oligo HPLC purified (new). The HPLC-purifed oligo depletes the mRNA more substantially ...
... was stripped and reprobed for a loading control, plakoglobin. (b) Northern blot of oocytes, either uninjected (Un) or injected with 2.5 or 5 ng of the antisense oligo used in Zhang et al. (1998) (old), or the same oligo HPLC purified (new). The HPLC-purifed oligo depletes the mRNA more substantially ...
SOX9 specifies the pyloric sphincter epithelium
... malrotation (Houston et al., 1983). Owing to the high mortality, only a few studies have addressed GI diseases or malformations in individuals with CD (Piper et al., 2002). In this study, we have investigated the expression and function of SOX9 in visceral pattern formation using the chick embryo as ...
... malrotation (Houston et al., 1983). Owing to the high mortality, only a few studies have addressed GI diseases or malformations in individuals with CD (Piper et al., 2002). In this study, we have investigated the expression and function of SOX9 in visceral pattern formation using the chick embryo as ...
neural crest specification: migrating into genomics
... break-up of ADHERENS and TIGHT JUNCTIONS, respectively. The ability of Slug/Snail to regulate the junctional proteins that are expressed in the neural crest has not been examined, but it is likely that they are also direct regulators of EMT in the neural crest. Curiously, Slug RNA is expressed in th ...
... break-up of ADHERENS and TIGHT JUNCTIONS, respectively. The ability of Slug/Snail to regulate the junctional proteins that are expressed in the neural crest has not been examined, but it is likely that they are also direct regulators of EMT in the neural crest. Curiously, Slug RNA is expressed in th ...
Artk96_CyclGMPReview_2016 - Pure
... Well established in animals, protozoa and prokaryotes, the signalling role of the cyclic nucleotide mono phosphates (cNMPs), cAMP and cGMP in plants was long doubted but the drastically improved analytical techniques have unequivocally shown the presence of both cAMP and cGMP in several plant specie ...
... Well established in animals, protozoa and prokaryotes, the signalling role of the cyclic nucleotide mono phosphates (cNMPs), cAMP and cGMP in plants was long doubted but the drastically improved analytical techniques have unequivocally shown the presence of both cAMP and cGMP in several plant specie ...
Dissecting stimulus-specific Ca2+ signals in amyloplasts and
... Calcium is used by plants as an intracellular messenger in the detection of and response to a plethora of environmental stimuli and contributes to a fine-tuned internal regulation. Interest in the role of different subcellular compartments in Ca2+ homeostasis and signalling has been growing in recen ...
... Calcium is used by plants as an intracellular messenger in the detection of and response to a plethora of environmental stimuli and contributes to a fine-tuned internal regulation. Interest in the role of different subcellular compartments in Ca2+ homeostasis and signalling has been growing in recen ...
Receptor Regulation of the Volume-Sensitive Efflux of Taurine and
... activity of protein kinase C (PKC). However, although PAR-1stimulated taurine efflux was attenuated by either a depletion of intracellular Ca2⫹ or inhibition of PKC by chelerythrine, the enhanced release of 125I⫺ was independent of both parameters. Stimulated efflux of 125I⫺ after activation of musc ...
... activity of protein kinase C (PKC). However, although PAR-1stimulated taurine efflux was attenuated by either a depletion of intracellular Ca2⫹ or inhibition of PKC by chelerythrine, the enhanced release of 125I⫺ was independent of both parameters. Stimulated efflux of 125I⫺ after activation of musc ...
Receptor Regulation of the Volume-Sensitive Efflux of Taurine and
... activity of protein kinase C (PKC). However, although PAR-1stimulated taurine efflux was attenuated by either a depletion of intracellular Ca2⫹ or inhibition of PKC by chelerythrine, the enhanced release of 125I⫺ was independent of both parameters. Stimulated efflux of 125I⫺ after activation of musc ...
... activity of protein kinase C (PKC). However, although PAR-1stimulated taurine efflux was attenuated by either a depletion of intracellular Ca2⫹ or inhibition of PKC by chelerythrine, the enhanced release of 125I⫺ was independent of both parameters. Stimulated efflux of 125I⫺ after activation of musc ...
Fibronectin and Other Adhesive Glycoproteins
... mass spectrometric techniques should allow localization of modifications to specific residues and may reveal additional sites of modification. ...
... mass spectrometric techniques should allow localization of modifications to specific residues and may reveal additional sites of modification. ...
The anaphase promoting complex/ cyclosome: a
... been suspected that the essential role of co-activator proteins in APC/C activation might be to recruit substrates to APC/C, analogously to the role of adaptor proteins in SCF complexes. This view was supported by the observations that Cdc20 and Cdh1 can confer a limited degree of substrate specific ...
... been suspected that the essential role of co-activator proteins in APC/C activation might be to recruit substrates to APC/C, analogously to the role of adaptor proteins in SCF complexes. This view was supported by the observations that Cdc20 and Cdh1 can confer a limited degree of substrate specific ...
Microbiology
... respectively, from their corresponding wild-type strains. In addition to complementation of the R-165 mutant with ompLC from Edw. ictaluri EILO, the R-165 mutant was also complemented with ompLC from Edw. ictaluri Alg-08-183, ML-08-116, ML-08-117 and Alg-08199. The ompLC gene with its predicted prom ...
... respectively, from their corresponding wild-type strains. In addition to complementation of the R-165 mutant with ompLC from Edw. ictaluri EILO, the R-165 mutant was also complemented with ompLC from Edw. ictaluri Alg-08-183, ML-08-116, ML-08-117 and Alg-08199. The ompLC gene with its predicted prom ...
Apical-basal patterning in Arabidopsis - Development
... along the stem was reduced (Przemeck et al., 1996). The deduced MP protein (Hardtke and Berleth, 1998) is related to a transcription factor, ARF, that binds to auxin-response elements (Ulmasov et al., 1997). These findings raised the ...
... along the stem was reduced (Przemeck et al., 1996). The deduced MP protein (Hardtke and Berleth, 1998) is related to a transcription factor, ARF, that binds to auxin-response elements (Ulmasov et al., 1997). These findings raised the ...