Lysis-deficient phages as novel therapeutic agents for controlling
... determination in clinical situations. A phage that kills the target cell but is incapable of host cell lysis would alleviate these concerns without compromising efficacy. Results: We developed a recombinant lysis-deficient Staphylococcus aureus phage P954, in which the endolysin gene was rendered no ...
... determination in clinical situations. A phage that kills the target cell but is incapable of host cell lysis would alleviate these concerns without compromising efficacy. Results: We developed a recombinant lysis-deficient Staphylococcus aureus phage P954, in which the endolysin gene was rendered no ...
Matching catalytic activity to developmental function: Tolloid
... In vertebrates, Bmp1/Tld-like proteases regulate the activity of Chordin, a Sog homolog, similar to the way Tld regulates Sog activity in Drosophila embryos. Bmp1 family members can process Chordin (Piccolo et al., 1997; Blader et al., 1997) and co-overexpression of vertebrate Tld proteases with Cho ...
... In vertebrates, Bmp1/Tld-like proteases regulate the activity of Chordin, a Sog homolog, similar to the way Tld regulates Sog activity in Drosophila embryos. Bmp1 family members can process Chordin (Piccolo et al., 1997; Blader et al., 1997) and co-overexpression of vertebrate Tld proteases with Cho ...
POPCORN Functions in the Auxin Pathway to Regulate
... characteristic of this mutant and propagated pcn and genetic combinations involving pcn by first growing the plants at 228C and then shifting them to 298C at bolting unless specified otherwise. To determine at which embryo stage PCN function is essential, pcn plants were grown at 228C, and flower bu ...
... characteristic of this mutant and propagated pcn and genetic combinations involving pcn by first growing the plants at 228C and then shifting them to 298C at bolting unless specified otherwise. To determine at which embryo stage PCN function is essential, pcn plants were grown at 228C, and flower bu ...
Development 128, 4439-4447 (2001)
... dissociated in Ca2+/Mg2+ free saline, incubated for 3 hours, and the secreted Chordin protein analyzed by western blot. Two affinity-purified antibodies were used, one raised against the central region of Chordin and one specific for the N terminus (Fig. 2A). Microinjection of Tsg mRNA caused a mark ...
... dissociated in Ca2+/Mg2+ free saline, incubated for 3 hours, and the secreted Chordin protein analyzed by western blot. Two affinity-purified antibodies were used, one raised against the central region of Chordin and one specific for the N terminus (Fig. 2A). Microinjection of Tsg mRNA caused a mark ...
1337 Biochemical properties of plasminogen activator inhibitor
... available in 1986 (16, 17). cDNA for human PAI-1 was cloned by 4 groups in 1986 (Figure 1) (18-21). The amino acid sequence of PAI-1 is now known in numerous mammalian species but in contrast, very little is known about the protein in non-mammalian species. In fact, an experimentally verified full-l ...
... available in 1986 (16, 17). cDNA for human PAI-1 was cloned by 4 groups in 1986 (Figure 1) (18-21). The amino acid sequence of PAI-1 is now known in numerous mammalian species but in contrast, very little is known about the protein in non-mammalian species. In fact, an experimentally verified full-l ...
Tissue interactions in the developing chick diencephalon
... free from basal neural tissue suggests that ZLI formation does not depend on ventral signals after HH10. We confirmed that ZLI formation can occur independently from ventral tissues in larger explants that encompassed the entire thalamic anlage (pro-Pth + pro-ZLI + pro-Th). Such explants expressed S ...
... free from basal neural tissue suggests that ZLI formation does not depend on ventral signals after HH10. We confirmed that ZLI formation can occur independently from ventral tissues in larger explants that encompassed the entire thalamic anlage (pro-Pth + pro-ZLI + pro-Th). Such explants expressed S ...
Leukotriene B4 Triggers the In Vitro and In Vivo
... 5-lipoxygenase and the leukotriene A4-hydrolase enzymes (for review see Ref. 22). LTB4 activates, through autocrine and paracrine mechanisms, several leukocyte types by binding and signaling through at least two distinct cell surface G protein-coupled receptors, BLT1 and BLT2 (23–25). The LTB4 recep ...
... 5-lipoxygenase and the leukotriene A4-hydrolase enzymes (for review see Ref. 22). LTB4 activates, through autocrine and paracrine mechanisms, several leukocyte types by binding and signaling through at least two distinct cell surface G protein-coupled receptors, BLT1 and BLT2 (23–25). The LTB4 recep ...
Ca signaling and early embryonic patterning during zebrafish
... primary germ layers and body axes. 3. Data are presented to support our proposal that intracellular Ca2+ waves are an essential feature of embryonic cytokinesis, and that propagating intercellular Ca2+ waves (both long and short range), may play a crucial role in: (a) the establishment of the embryo ...
... primary germ layers and body axes. 3. Data are presented to support our proposal that intracellular Ca2+ waves are an essential feature of embryonic cytokinesis, and that propagating intercellular Ca2+ waves (both long and short range), may play a crucial role in: (a) the establishment of the embryo ...
Biological effects of Extracellular fibrinogen binding protein (Efb) in
... platelet, which did not involve the normal platelet fibrinogen receptor, GPIIb/IIIa. We propose that fibrinogen can also bind to the platelet via Efb and this generates the novel fibrinogen binding mediated by Efb. In the presence of Efb, platelet activation was diminished and Efb also inhibited pla ...
... platelet, which did not involve the normal platelet fibrinogen receptor, GPIIb/IIIa. We propose that fibrinogen can also bind to the platelet via Efb and this generates the novel fibrinogen binding mediated by Efb. In the presence of Efb, platelet activation was diminished and Efb also inhibited pla ...
Interrelations between translation and general mRNA degradation in
... decay en masse. Finally, recent results suggest that mRNA decay is not limited to non-translating messages and can occur when ribosomes are not initiating but are still elongating on mRNA. This review will discuss the models for the mechanisms of these processes and recent developments in understand ...
... decay en masse. Finally, recent results suggest that mRNA decay is not limited to non-translating messages and can occur when ribosomes are not initiating but are still elongating on mRNA. This review will discuss the models for the mechanisms of these processes and recent developments in understand ...
Superinfection Exclusion by P22 Prophage in Lysogens of Salmonella typhimurium. II. Genetic Evidence for Two Exclusion Systems.
... plate P22vir-3 with high efficiencies, but they still exclude MG178c about as well as t’he wild-type lysogen. LcII phage plate on these lysogens with an intermediate e.o.p. of 10m3, or KFfold higher t’han on t’he wild-type lysogen. As shown below, bhis phenotype apparently results from mutation in a ...
... plate P22vir-3 with high efficiencies, but they still exclude MG178c about as well as t’he wild-type lysogen. LcII phage plate on these lysogens with an intermediate e.o.p. of 10m3, or KFfold higher t’han on t’he wild-type lysogen. As shown below, bhis phenotype apparently results from mutation in a ...
Donohoe, B.S., B. - University of Colorado-MCDB
... The alternative ‘dock, pluck and go’ model (30) postulates that the coupling of Golgi stacks to ER export sites in plant cells is transient, and occurs only when an ER export site is actively producing COPII buds and vesicles for export to the Golgi. To this end, budding COPII vesicles are born with ...
... The alternative ‘dock, pluck and go’ model (30) postulates that the coupling of Golgi stacks to ER export sites in plant cells is transient, and occurs only when an ER export site is actively producing COPII buds and vesicles for export to the Golgi. To this end, budding COPII vesicles are born with ...
Plasma Gelsolin
... Both the cytoplasmic and plasma isoforms of gelsolin have at least three Ca2+-binding sites of nanomolar and micromolar affinity [17, 22], but gelsolin binds only two calcium ions in the presence of actin, and they bind cooperatively [23, 24] Fig. (1). A recent study reports a high resolution struct ...
... Both the cytoplasmic and plasma isoforms of gelsolin have at least three Ca2+-binding sites of nanomolar and micromolar affinity [17, 22], but gelsolin binds only two calcium ions in the presence of actin, and they bind cooperatively [23, 24] Fig. (1). A recent study reports a high resolution struct ...
RECENT ADVANCES IN COTTON FIBER DEVELOPMENT
... Transcriptional Regulation of Initiation Transcription is regulated by various small RNAs, such as small interfering RNAs (siRNAs), as well as the activity of transcription factors. Small RNAs occur in size classes that regulate gene expression differently. siRNAs are predominantly 24 nucleotides lo ...
... Transcriptional Regulation of Initiation Transcription is regulated by various small RNAs, such as small interfering RNAs (siRNAs), as well as the activity of transcription factors. Small RNAs occur in size classes that regulate gene expression differently. siRNAs are predominantly 24 nucleotides lo ...
Leukocyte lipid bodies — Biogenesis and functions in inflammation
... of the ER bilayer (Figure 2 A). After reaching a certain size, nascent lipid bodies loaded with proteins lacking trans-membrane spanning domains bud off the ER into the cytoplasm that ends up surrounded by a half-unit membrane of phospholipids directly derived from the cytoplasmic leaflet of the ER ...
... of the ER bilayer (Figure 2 A). After reaching a certain size, nascent lipid bodies loaded with proteins lacking trans-membrane spanning domains bud off the ER into the cytoplasm that ends up surrounded by a half-unit membrane of phospholipids directly derived from the cytoplasmic leaflet of the ER ...
E7.5 endoderm induction by adjacent germ layers
... 1997; St-Onge et al., 1997; Sussel et al., 1998). How these transcription factors interact to generate specific cell types is as yet unclear, although some target genes of Pdx1, including insulin and somatostatin (Goudet et al., 1999), have been identified. In the studies mentioned above, particular ...
... 1997; St-Onge et al., 1997; Sussel et al., 1998). How these transcription factors interact to generate specific cell types is as yet unclear, although some target genes of Pdx1, including insulin and somatostatin (Goudet et al., 1999), have been identified. In the studies mentioned above, particular ...
Neurotransmitter signaling through heterotrimeric G
... Neurotransmitters signal via two distinct classes of receptors, known within the neuroscience field as ionotropic and metabotropic receptors. Ionotropic receptors are neurotransmitter-gated ion channels, and most small-molecule neurotransmitters each have a number of such receptors (Lemoine et al., ...
... Neurotransmitters signal via two distinct classes of receptors, known within the neuroscience field as ionotropic and metabotropic receptors. Ionotropic receptors are neurotransmitter-gated ion channels, and most small-molecule neurotransmitters each have a number of such receptors (Lemoine et al., ...
Slow Ventricular Conduction in Mice Heterozygous for a
... The genotypes of all mice were determined by PCR, using primer sequences and protocols identical to those described by Reaume et al. (8) in their original report of the Cx43 2/2 mouse. Conduction studies. Conduction velocity was measured on the ventricular epicardial surface in superfused neonatal h ...
... The genotypes of all mice were determined by PCR, using primer sequences and protocols identical to those described by Reaume et al. (8) in their original report of the Cx43 2/2 mouse. Conduction studies. Conduction velocity was measured on the ventricular epicardial surface in superfused neonatal h ...
Pathways of NeurSospora crassa and Saccharomyces cerevisiaet
... encodes the enzyme proteins. The genetic work, together with more recent evidence, suggests that the kinase and reductase are derived from a single polypeptide. Over 60 mutant alleles of the S. cerev'isiae ARG5,6 locus could be classified into three complementation groups (125, 170). One group (arg6 ...
... encodes the enzyme proteins. The genetic work, together with more recent evidence, suggests that the kinase and reductase are derived from a single polypeptide. Over 60 mutant alleles of the S. cerev'isiae ARG5,6 locus could be classified into three complementation groups (125, 170). One group (arg6 ...
Sears JC and Broihier HT
... extensive branching suggests that anterograde MT polymerization may play a role in generating dendrite arbors. This hypothesis is supported by recent studies demonstrating a function for anterograde MT polymerization in facilitating nascent branch formation and stabilization (Ori-McKenney et al., 20 ...
... extensive branching suggests that anterograde MT polymerization may play a role in generating dendrite arbors. This hypothesis is supported by recent studies demonstrating a function for anterograde MT polymerization in facilitating nascent branch formation and stabilization (Ori-McKenney et al., 20 ...
Running Head: Superresolution of cortical
... Since details of microtubule organization have been reported using other microscopies, the first ...
... Since details of microtubule organization have been reported using other microscopies, the first ...
VARICOSE, a WD-domain protein, is required for leaf
... defects (Okada et al., 1991; Ruegger et al., 1997). Leaf development is also perturbed by growing wild-type plants in the presence of polar auxin transport inhibitors (Mattsson et al., 1999; Sieburth, 1999). Furthermore, some auxin-resistant mutants, such as axr1 and axr2-1, have disrupted auxin res ...
... defects (Okada et al., 1991; Ruegger et al., 1997). Leaf development is also perturbed by growing wild-type plants in the presence of polar auxin transport inhibitors (Mattsson et al., 1999; Sieburth, 1999). Furthermore, some auxin-resistant mutants, such as axr1 and axr2-1, have disrupted auxin res ...
presence of allhnase in isolated vacuoles and of alkyl
... Abbreviations: DTT, dithiothreitoi; FDA, fluorescein diaeetate; TLC, thin-layer chromato- ...
... Abbreviations: DTT, dithiothreitoi; FDA, fluorescein diaeetate; TLC, thin-layer chromato- ...
Spatial Reorganization of Glycogen Synthase upon Activation in 3T3
... (Herndon, VA), and fetal bovine serum (FBS) was obtained from Hyclone (Logan, UT.) All other chemicals were from Sigma (St. Louis, MO). UDP-[U-3H]glucose (60 Ci/mmol) was supplied by American Radiolabeled Chemicals (St. Louis, MO). ECL reagent was purchased from Amersham Pharmacia Biotech (Uppsala, ...
... (Herndon, VA), and fetal bovine serum (FBS) was obtained from Hyclone (Logan, UT.) All other chemicals were from Sigma (St. Louis, MO). UDP-[U-3H]glucose (60 Ci/mmol) was supplied by American Radiolabeled Chemicals (St. Louis, MO). ECL reagent was purchased from Amersham Pharmacia Biotech (Uppsala, ...
Effect of bile salts on the DNA and membrane integrity of enteric
... dangerous environments encountered in the gastrointestinal tract. This review will focus on several recently discovered mechanisms that allow for protection and continued proliferation in the stressful environment of high concentrations of bile salts for the Gram-negative enteric pathogenic bacteria ...
... dangerous environments encountered in the gastrointestinal tract. This review will focus on several recently discovered mechanisms that allow for protection and continued proliferation in the stressful environment of high concentrations of bile salts for the Gram-negative enteric pathogenic bacteria ...