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Seed-coat Dormancy in Grevillea linearifolia
Seed-coat Dormancy in Grevillea linearifolia

... † Background and Aims Seeds of Grevillea linearifolia germinate following fire, and have seed-coat dormancy broken by smoke and heat shock. Smoke breaks seed coat dormancy in Emmenanthe penduliflora by altering the permeability of the seed coat to an internal germination inhibitor, which subsequentl ...
Muscle precursor cells isolated from aged rats exhibit
Muscle precursor cells isolated from aged rats exhibit

... These findings are significant due to the fact that TRAF-2 is both involved as a key adapter molecule for signaling downstream of TNFR1 and TNFR2 and a known target of NF-κB (Gupta & Gollapudi, 2005). We next measured the signaling events downstream of TNF receptors and their adaptor proteins. IκBα ...
Analysis of Connexin43 phosphorylated at S325, S328 and S330 in
Analysis of Connexin43 phosphorylated at S325, S328 and S330 in

... although development of coupling was delayed. Fully opened channels were only rarely observed in Cx43-TM cells, and Lucifer-Yellow-dye-coupling was significantly reduced compared with wild-type cells. These data suggest that phosphorylation of Cx43 at serine residues 325, 328 and/or 330 influences c ...
Journal of Bacteriology
Journal of Bacteriology

... investigated during various phases of bacterial growth in yeast extract-mannitol medium. Direct cell counting revealed that growth of the rhizobia transiently stopped three successive times during batch culture in yeast extract-mannitol medium. These interruptions of growth, as well as the simultane ...
XMNfRenal_Transplant
XMNfRenal_Transplant

... nephrectomy is a study of intraperitoneal and retroperitoneal anatomy, and each living donor nephrectomy is a surgical procedure on a kidney, procedures that are decreasing in frequency because of the development of nonoperative methods of treating renal stones and obstruction. Nowhere in urology mu ...
Curriculum Guide Template DRAFT
Curriculum Guide Template DRAFT

... Goal 2: Learner will develop an understanding of the physical, chemical and cellular basis of life. Objective: 2.03 Investigate and analyze the cell as a living system including: a. Maintenance of homeostasis. b. Movement of materials into and out of cells. c. Energy use and release in biochemical r ...
Quantifying bacteria internalization by macrophage
Quantifying bacteria internalization by macrophage

... Streptococcus agalactiae (Group B Streptococcus, GBS) causes serious invasive infections in newborns and adults, particularly in the elderly and individuals with underlying chronic disease. These infections are hard to fight because GBS has evolved several ways to evade the first line of immune defe ...
Suppressor analysis of the protein kinase Elm1p, an enzyme
Suppressor analysis of the protein kinase Elm1p, an enzyme

... known as invasive growth (116). Haploid cells undergoing this differentiation alter their bud site selection, slightly elongate, and require many of the same elements used in the mating MAP kinase cascade. Diploid yeast cells can enter an alternative growth mode when there are adequate sources of ca ...
Body Organization
Body Organization

... divides into a small cluster of cells. After about 5 days, a small ball of a few hundred cells is formed, which encloses a mass of embryonic stem cells. These early, undifferentiated cells will give rise to all of the types of cells of the developing body. Embryonic stem cells are immortal—that is, ...
Ca2+ signalling and control of guard-cell volume in stomatal
Ca2+ signalling and control of guard-cell volume in stomatal

... in the past four years, have yielded some of the most exciting new findings in plant cell signalling. Stomatal movements are achieved through changes in guard-cell volume, which, in turn, are driven by the accumulation (during opening) and loss (during closing) of osmotically active solutes (i.e. KC ...
isolation and characterization of membranes from the cells of maize
isolation and characterization of membranes from the cells of maize

... in 18% (w/v) trichloroacetic acid. The remainder fraction was precipitated with 18% (w/v) trichloroacetic acid. The insoluble material was washed 4 times with 18 % (w/v) trichloracetic acid followed by acetone which was removed by heating in an oven at 60 °C for 5 min. It was then dissolved in 50 fi ...
Escaping Underground Nets: Extracellular DNases Degrade
Escaping Underground Nets: Extracellular DNases Degrade

... The growing tip of a plant root is uniquely vulnerable to infection as it moves through the dense microbial community of the soil, unprotected by cuticle or bark. However, roots are defended by tiles of loosely attached secretory cells called root border cells, which produce a matrix of proteins, po ...
Neurofilaments Help Maintain Normal Morphologies and Support
Neurofilaments Help Maintain Normal Morphologies and Support

... antiserum to localize XNIF in cells injected with FITC-DX required modifications in the fixation protocols, since the epitope of the XNIF antiserum is sensitive to aldehydes, which were necessary for proper fixation of the lysinated dextran. Cultures were first fixed by dropwise addition of methanol ...
Functional Analysis of Cellulose and Xyloglucan
Functional Analysis of Cellulose and Xyloglucan

... stomata, we measured CSC density and speed in stomatal guard cells from 6-d-old seedlings expressing GFP-CESA3 in the cesa3je5 mutant background (Desprez et al., 2007) using time-lapse live-cell imaging. Young seedlings were used in this experiment because preliminary analyses demonstrated that ther ...
Effects of Human Ageing on Periodontal Tissues
Effects of Human Ageing on Periodontal Tissues

Characterization of cell mismatch in photovoltaic modules using
Characterization of cell mismatch in photovoltaic modules using

... eye, to be detected allowing the reduced performance observed in I-V curves to be explained. It was seen that the cracked cells have a significant effect on the current produced by a string, while the effect of delaminated areas is less severe. Hot spots are observed on weak cells indicating they ar ...
Vertebrate limb development--the early stages in chick - ICB-USP
Vertebrate limb development--the early stages in chick - ICB-USP

... phenotype was reported a few years ago in Fgf10 knockouts. Both forelimbs and hindlimbs are almost completely absent [2,3] and, depending on genetic background, no buds form at all or limb buds are initiated but do not grow out. However, importantly, in both cases, the apical ectodermal ridge does n ...
Many ways to telomere dysfunction: in vivo studies using
Many ways to telomere dysfunction: in vivo studies using

... Department of Immunology and Oncology, Centro Nacional de BiotecnologõÂa-CSIC, Campus Cantoblanco, E-28049, Madrid, Spain ...
1 The LF1 Gene of Chlamydomonas reinhardtii Encodes
1 The LF1 Gene of Chlamydomonas reinhardtii Encodes

... deflagellation, more than 35% of the lf1 cells remained aflagellate (data not shown) indicating that in addition to the flagellar length defect, these cells have either a flagellar assembly defect or a defect in the response to flagellar amputation. The lf1 mutation also resulted in abnormal cell mo ...
Reciprocal regulation of IL-23 and IL-12 following co
Reciprocal regulation of IL-23 and IL-12 following co

... in DC has been demonstrated before [8, 10], our data differ from previous findings in that we show that optimal IL-10, IL-23 and IL-6 production requires co-ligation of Dectin-1 and TLR, as opposed to ligation of Dectin-1 alone. Our data compliment recent studies that demonstrated that large particu ...
Guidelinesrelating to solid organ transplants from non
Guidelinesrelating to solid organ transplants from non

... regarding suitability for organ donation. The potential for tissue donation should be explored with competant authorities. If next of kin or family are present a verbal lack of objection will be obtained prior to cannula insertion and perfusion. If family members are not present cannulation can stil ...
Temporal and Spatial Distribution of DNA Topoisomerase II Alters
Temporal and Spatial Distribution of DNA Topoisomerase II Alters

... changes in DNA topology by passing a double-stranded DNA helix through a transient double-strand break site and then rejoining the strand break.1,2 Conditional yeast mutants in the top2 gene showed that this enzymatic activity is required for segregation of daughter chromosomes during anaphase.3 Bio ...
Do Plant Cell Walls Extend?
Do Plant Cell Walls Extend?

... chamber and applying the minimum air pressure required to block cell extension. This is called the pressure-block method. As the walls relax, greater pressure must be applied to block the cells from taking up water and extending. These relaxation methods confirmed experimentally that cell enlargemen ...
Universitas Scientiarum
Universitas Scientiarum

... Because the use of bacteria for biotechnological processes requires maintaining their viability and genetic stability, preserving them becomes essential. Here, we evaluated three preservation methods for A. chroococcum C26 and A. vinelandii C27; preservation methods: cryopreservation and immobilizat ...
Lecture Exam 3 Study Guide Sponges 1. Explain why sponges are
Lecture Exam 3 Study Guide Sponges 1. Explain why sponges are

... Homeostatic Mechanisms and Energy Balance 17. Explain where and in what form energy rich molecules may be stored in the human body. 18. Explain why fat hoarding may have provided a fitness advantage to our hunter-gatherer ancestors. CHAPTER 42 CIRCULATION AND GAS EXCHANGE Circulatory Systems of Anim ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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