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Exosome Biogenesis, Regulation, and Function in Viral Infection
Exosome Biogenesis, Regulation, and Function in Viral Infection

... of mechanisms sustaining packaging of membrane-bound and soluble material to these vesicles and the understanding of the underlying processes directing MVBs for degradation or fusion with the plasma membrane. The investigation into the formation and roles of exosomes in viral infection is in its ear ...
Glycosyltransferase activity of Fringe modulates
Glycosyltransferase activity of Fringe modulates

... Notch–AP produced in cells co-expressing Fringe–GT–myc bound to Delta-expressing cells 20 times more effectively than Notch–AP produced in the absence of Fringe (Fig. 4b). This suggests that the observed binding relies solely on the EGF modules of Notch being present as a secreted soluble protein. T ...
Chapter 42 ppt
Chapter 42 ppt

... Concept 42.1: Circulatory systems link exchange surfaces with cells throughout the body • In small and/or thin animals, cells can exchange materials directly with the surrounding medium • In most animals, transport systems connect the organs of exchange with the body cells • Most complex animals ha ...
Molecular Imaging of the Initial Inflammatory Response in
Molecular Imaging of the Initial Inflammatory Response in

... initiation and progression of atherosclerosis and the susceptibility to acute ischemic syndromes. Novel methods for detecting vascular inflammation are being developed to better ascertain risk for adverse events. One strategy is to directly visualize the molecular or cellular components of the immun ...
Starvation Induces Vacuolar Targeting and Degradation of the
Starvation Induces Vacuolar Targeting and Degradation of the

... els of GAP1 transcription and GAP1 sorting and degradation (Stanbrough and Magasanik, 1995; Roberg et al., 1997b; Springael and Andre, 1998). The second class of amino acid permeases consists of mainly high affinity transporters that are specific for single amino acids or a small set of structurally ...
Subject Materials for Biology
Subject Materials for Biology

... Q7 .How does amoeba engulfs its food? Ans: Amoeba obtains food by phagocytosis. Amoeba engulfs the food by forming pseudopodia. Two pseudopodia start growing from the opposite sides of amoeba. They completely encircle the food and their lips touch each other. The membranes of both the pseudopodia di ...


... steps: protrusion of the front end, translocation of the cell body, and retraction of the rear end. For cells to migrate efficiently, these steps need to be well organized, and the actin cytoskeleton plays a critical role in each step. One of the actin structures important for cell motility is the s ...
The engrailed homeobox genes are required in multiple cell
The engrailed homeobox genes are required in multiple cell

... Available online 28 April 2012 ...
Celiac trunk: anatomical variants and pathological findings at MDCT
Celiac trunk: anatomical variants and pathological findings at MDCT

... Anatomical variants Anatomical variants of the celiac trunk were first classified by Adachi in 1928, based on 252 dissections of Japanese cadavers, where six types of divisions of the celiac trunk and superior mesenteric artery were described. In 1955, Michel created another classification, also inc ...
Identity and activity of marine microbial populations as
Identity and activity of marine microbial populations as

... activity. These approaches should optimally provide a resolution at the level of single populations or even cells as bulk activity measurements seldom correlate with total abundances of bacteria and specific microbial populations may mediate central biogeochemical processes. At the end of the 1990’s ...
FGF1 inhibits p53-dependent apoptosis and cell cycle arrest via an
FGF1 inhibits p53-dependent apoptosis and cell cycle arrest via an

... FGF1 inhibits p53-dependent apoptosis We investigated the relevance of fgf1 gene repression in the p53 pathway by assessing the impact of this factor on p53-dependent apoptosis and cell growth arrest. We transfected REtsAF cells with a pSVL-FGF1 expression vector (p267 (Jaye et al., 1988; Renaud et ...
PDF - Hormones.gr
PDF - Hormones.gr

... Figure 1. Autophagosmes and lysosomes. LC3 (green), a marker of mature autophagosomes, is not localized on CD63 (red) labeled organelles. CD63 primarily resides on lysosomes and plasma membrane. As yet, no reliable markers exist to monitor the transition to autophagolysosomes. ...
acid-base disturbances
acid-base disturbances

... • Hypoaldo (Type IV): ↑ K → ↓ NH3 synthesis/delivery → ↓ urine acid carrying capacity ...
BIOL 105 Example Midterm Exam 1 QA 140310
BIOL 105 Example Midterm Exam 1 QA 140310

... MUSCULAR SYSTEM ...
pdf, 1.1 MB - The Nebenführ Lab
pdf, 1.1 MB - The Nebenführ Lab

... Golgi stacks in different regions of the cells with the available volume of cytoplasm. For this purpose, cytoplasm was defined as the area covered by the combined fluorescence of our GFP-marker, which stains the Golgi and to some extent, the ER (Nebenführ et al., 1999), and the MitoTracker dye, whi ...
Discovery of the cardiovascular system: from
Discovery of the cardiovascular system: from

... circulation changed the intellectual system and worldview of physiology, disease and therapy. As modern-day clinician-scientists, why should we care about the history of the circulation? For one, the historical account reminds us that investigators from different eras should be judged in the context ...
Plant tropisms - Semantic Scholar
Plant tropisms - Semantic Scholar

... relative to an opposing position within that same organ. As most work in the area of tropic response has shown, curvature can only be properly manifested through the coordinated activity of hormones. Small fluctuations in the cellular concentration of hormones can have a drastic effect on whether or ...
Saccharomyces cerevisiae
Saccharomyces cerevisiae

... obtained higher enzyme yields. Of the different concentrations tested we obtained best results with 1 M-sucrose (10-20 times more activity than controls). Addition of 10% glycerol to breakage medium gave intermediate protection, whereas addition of sucrose after cell breakage was completely ineffect ...
Cell-cycle-regulated expression of STIL controls centriole number in
Cell-cycle-regulated expression of STIL controls centriole number in

... assembly of a so-called cartwheel structure, which presumably serves as an assembly platform for the outgrowth of the procentriole. Importantly, SAS-6 has recently been identified as a key component of the cartwheel (Nakazawa et al., 2007) and shown to confer ninefold symmetry to this structure (Kit ...
A Cellular Hypothesis for the Induction of Blossom
A Cellular Hypothesis for the Induction of Blossom

...  Background The incidence of blossom-end rot (BER) is generally associated with a calcium (Ca) deficiency in the distal portion of tomato fruits. The visible symptom is a necrotic lesion, which is presumed to be a consequence of cell death and the subsequent leakage of solutes into the extracellula ...
Mosses as model systems for the study of metabolism and
Mosses as model systems for the study of metabolism and

... their growth media (120, 122). Complementation analysis between 15 OVE mutants showed that 14 are recessive, and thus likely to be loss of function mutants. Seven of these mutants were grouped into three complementation groups (44). The identities of these genes remain to be elucidated. The isolatio ...
spp. Listeria Freeze-Thaw Tolerance of Role of Growth
spp. Listeria Freeze-Thaw Tolerance of Role of Growth

... and H7550, respectively, suggesting significantly lower cryotolerance of F2365 (P ⬍ 0.05) (Fig. 2). Plating of cell suspensions of F2365 (grown at 37 and 4°C in liquid and subjected to repeated freezing and thawing) on TSAYE and on modified Oxford selective medium did not provide evidence for injure ...
Origin of the zebrafish endocrine and exocrine
Origin of the zebrafish endocrine and exocrine

... model for the study of vertebrate organogenesis, combining powerful genetics with accessible, optically clear embryos that allow cell fates and movements to be traced. In recent years, several studies have revealed that the zebrafish pancreas shares many properties with the mammalian pancreas. Thus, ...
Effects of Ammonia and Lactate on Hybridoma Growth, Metabolism
Effects of Ammonia and Lactate on Hybridoma Growth, Metabolism

... metabolic deamination of glutamine to glutamate and by the conversion of glutamate to a-ketoglutarate. Concentration of ammonia in cell culture is influenced by the mode of reactor operation, cell and glutamine concentrations, and cellular activities. Although ammonia concentrations of 2-5 mM are ty ...
clinical flow cytometry – emerging applications
clinical flow cytometry – emerging applications

... Advances in patient management have often been closely linked to the development of critical quantitative analysis methods. Flow cytometry is such an important methodology. It can be applied to individual cells or organelles allowing investigators interested in obtaining information about the functi ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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