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Diapositive 1
Diapositive 1

... Figure S3. Rodent Par-4 is phosphorylated by CK2 at residues S124 and S223 (A, B) The phosphopeptides observed in Fig.2A (phosphorylated by the recombinant CK2) were analyzed as described in Material and Methods by MALDI-TOFF mass spectrometry (A) and Edman degradation (B), thus identifying S124 and ...
Multiple mechanisms determine the order of APC/C substrate
Multiple mechanisms determine the order of APC/C substrate

... variability in the timing of APC/CCdc20 activation and anaphase onset. This timing and variability did not change significantly in any of the strains carrying GFP-tagged APC/C substrates (Fig. 2 A, one-way ANOVA, P = 0.47), which is consistent with the fact that GFP tagging had no effect on the doub ...
Emergency Care and Transportation of the Sick and Injured, Tenth
Emergency Care and Transportation of the Sick and Injured, Tenth

... 3. The cardiovascular system requires three components to keep the blood moving: a. Functioning pump (heart) b. Adequate fluid volume (blood and body fluids) c. Intact system of tubing capable of reflex adjustments in response to changes in pump output and fluid volume (blood vessels) 4. The heart’s ...
biology syllabus
biology syllabus

... Outline the role of condensation and hydrolysis in the relationships between monosaccharides, disaccharides and polysaccharides; between fatty acids, glycerol and triglycerides; and between amino acids and polypeptides. ...
Developing Technology-Based Biology Assessments for Cell
Developing Technology-Based Biology Assessments for Cell

... necessary connections between the structures and functions of cells. For a more comprehensive understanding, they need to grasp the concept of hierarchical organization of these microscopic entities (Berthelsen, 1999). Furthermore, many students think of organisms in terms of containing cells rather ...
Post renal oliguria
Post renal oliguria

... drugs in the dehydrated, prerenal patient. The most common cause of perioperative ARF is ATN caused by ischemia. Although this process is commonly called “necrosis”, tubular epithelial cell loss after ischemia results from both necrosis and apoptosis. Damaged tubular cells slough and obstruct the na ...
Ultrastructural immunocytochemical localization of
Ultrastructural immunocytochemical localization of

... controls were run: (i) fibronectin antiserum replaced by normal rabbit serum; (ii) fibronectin antiserum replaced by PBS; and (iii) all steps up to the DAB reagent replaced with PBS + g.s., with a wash in PBS alone immediately before the DAB treatment. Penetration of the PAP reagents into the tissue ...
Study of reactive oxygen species-induced ion transport in different models by using
Study of reactive oxygen species-induced ion transport in different models by using

... first group glutathione subcellular glutathione ...
Fig. 1 - The Journal of Cell Biology
Fig. 1 - The Journal of Cell Biology

... in the inner mitochondrial membrane and regulates gating of the Tim23 pore. A surprising and still unresolved observation is that a loss of Tim50 leads to robust import defects for matrix proteins, but has a much lesser effect on precursors sorted into the inner membrane (Geissler et al., 2002). To ...
A Role for the Cytoskeleton in Heart Looping
A Role for the Cytoskeleton in Heart Looping

... activating factors that can bind to cell surface receptors, as well as lead to ECM degradation. N-cadherin/βcatenin complex functions in association with connexins and regulates cyclic stretch by its interactions with the cytoskeleton, as well as neighboring cells through mediation of cell-cell adhe ...
Use of Malyugin pupil expansion device for IFIS
Use of Malyugin pupil expansion device for IFIS

... Inc.) and the Perfect Pupil (Milvella Ltd.) are disposable poly(methyl methacrylate) and polyurethane, respectively, pupil expansion rings.12 They are inserted with the use of a reusable metal injector but have a higher vertical profile within the anterior chamber than the Malyugin ring. Because of ...
Syntaxin of Plant Proteins SYP123 and SYP132 Mediate Root Hair
Syntaxin of Plant Proteins SYP123 and SYP132 Mediate Root Hair

... BFA is known to interfere with the recycling of several PM proteins, inhibiting the activity of the exchange factors for ARF GTPases (ARF-GEFs) and forming large aggregations called ‘BFA compartments’ inside cells (Satiat-Jeunemaitre et al. 1996, Staehelin and Driouich 1997). We treated the growing ...
Emergent Synchronous Bursting of Oxytocin Neuronal Network
Emergent Synchronous Bursting of Oxytocin Neuronal Network

... but within a bundle the dendrites are directly apposed to each other [4,14,15]. Dendritic oxytocin release in basal conditions in vivo is not much influenced by spike activity, but can be evoked by stimuli that mobilize intracellular Ca2+ [16]. When oxytocin is released, it acts at high-affinity rec ...
the physiology of mechanoelectrical transduction channels in hearing
the physiology of mechanoelectrical transduction channels in hearing

... of ion channel have been extensively characterized at a biophysical and molecular level over the last 20 years (98). They are typically composed of four to five membranespanning protein subunits clustered around a central aqueous conduit that can be opened or closed by the appropriate electrical or ...
worm - Montville.net
worm - Montville.net

... Worms have many unique characteristics different from sponges and cnidarians. ...
View PDF - G3: Genes | Genomes | Genetics
View PDF - G3: Genes | Genomes | Genetics

... 2). A 1:70 dilution was a pragmatic choice of dilution in the range used in low-throughput experiments. In low-throughput experiments where we carefully count cell number at each passage and dilute back to 5 · 105 cells per milliliter every day (Figure S3), the dilution ranges from 1:600 for healthy ...
Cells To Tolerate Higher Expression of Bim T+ Bcl
Cells To Tolerate Higher Expression of Bim T+ Bcl

... death of effector T cells (21). Recent work, however, has suggested a potentially more complex interplay between Bim and Bcl-2 (22), as it was shown that genetic ablation of Bim leads to decreased levels of Bcl-2 protein, whereas overexpression of Bcl-2 promotes increased expression of Bim mRNA and ...
CHAPTER
CHAPTER

... 20 per cent of the body weight, or about 14 liters in a normal 70-kilogram adult. The two largest compartments of the extracellular fluid are the interstitial fluid, which makes up more than three fourths of the extracellular fluid and the plasma, which makes up almost one fourth of the extracellula ...
PI3K and Rho GTPase activity are required for exogenous PIP3
PI3K and Rho GTPase activity are required for exogenous PIP3

... and independent of cortical actin polymerization and PI3K activity (e.g. Ras is the BSPI3K in the LEGI model). 2. Ras recruits and activates a small fraction of PI3K at the plasma membrane, resulting in AKT activation and other responses (including a secondary Rac activation-see below). 3. In parall ...
CHAPTER
CHAPTER

... 20 per cent of the body weight, or about 14 liters in a normal 70-kilogram adult. The two largest compartments of the extracellular fluid are the interstitial fluid, which makes up more than three fourths of the extracellular fluid and the plasma, which makes up almost one fourth of the extracellula ...
Barbiturates Directly Inhibit the Calmodulin/Calcineurin Complex: a
Barbiturates Directly Inhibit the Calmodulin/Calcineurin Complex: a

... specific band (data not shown). Moreover, inhibition of the DNA binding activity of other transcription factors, such as Oct-1, SP-1, or CREB, in response to barbiturates was variable and only marginal compared with the binding of nuclear proteins to the NFAT probe (Fig. 2), thus indicating a prefer ...
Symplasmic networks in secondary vascular tissues
Symplasmic networks in secondary vascular tissues

... leaves and terminal sinks (root, fruit, or seed) are separated by a great distance, while a strong axial sink, the vascular cambium and its rapidly expanding derivatives, is just several cells away from the phloem along the entire length of the stem. Parenchyma storing carbohydrates in both xylem an ...
Liver development
Liver development

... of gestation (e8.0) indicate that the embryonic liver originates from the ventral foregut endoderm (Tremblay and Zaret, 2005). The first morphological sign of the embryonic liver is the formation of the hepatic diverticulum, an out-pocket of thickened ventral foregut epithelium adjacent to the devel ...
l.The Respiratory System 2404
l.The Respiratory System 2404

... 5. lung cancer uncontrolled growth of cells crowd out normal cells ...
Modeled Osteopathic Manipulative Treatments: A Review of Their in
Modeled Osteopathic Manipulative Treatments: A Review of Their in

... Repetitive and forceful movements, awkward postures, and sustained forces often lead to repetitive motion strain (RMS), a common condition generally correlated to occupationrelated factors such as physical and psychological distress and monotonous work, but which is also correlated to non–work relat ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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