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Development of bacterial oxidative stress assays
Development of bacterial oxidative stress assays

... cytotoxicity of an antibiotic, a dose-response curve can be generated by counting the number of CFUs remaining after incubation with a range of concentrations of the antibiotic in question. The optical density of the cells is measured using a spectrophotometer. However, both cells that are dead and ...
AtLSG1-2 Regulates Leaf Growth by Affecting Cell Proliferation and
AtLSG1-2 Regulates Leaf Growth by Affecting Cell Proliferation and

... were suppressed in the atlsg1-2 mutant. Because cell expansion is closely related with the endoreduplication process, we examined the ploidy distribution by flow cytometry in the first pair of wild-type and atlsg1-2 mutant leaves. Leaf samples were collected during cell division, cell expansion, and ...
The functions of the cytoskeleton and associated
The functions of the cytoskeleton and associated

... thaliana (Azimzadeh et al., 2008). TON1 Recruiting Motif proteins (TRMs) have been recently shown to interact with TON1 in Arabidopsis. One of TRMs (TRM1) is found to bind and recruit TON1 to the cortical MTs (Drevensek et al., 2012). Recently, it has been reported that the activity of a regulatory ...
3. Taxonomy and classification of Algae
3. Taxonomy and classification of Algae

... empty in the light microscope (whereas akinetes appear full of storage products). Heterocysts have been drastically altered to provide the necessary anoxygenic environment which is ideal for nitrogenase enzyme necessary for the process of nitrogen fixation as following:. o They are photosyntheticall ...
Leukemia Inhibitory Factor (LIF) is Produced by Myelin Reactive T
Leukemia Inhibitory Factor (LIF) is Produced by Myelin Reactive T

... chronic and a relapsing-remitting mouse EAE model (Butzkueven et al., 2002). The favorable clinical effect of LIF could be associated with increased oligodendrocyte survival in vivo and was unrelated to effects on inflammation or immune responses. In CNTF-deficient mice, EAE induction led to earlier ...
performance of nonlinear visual units in ocular hypertension
performance of nonlinear visual units in ocular hypertension

... (1983) should be consulted for an introduction to the use of this package and for a list of similar gratings we first submitted the six thresholds packages. Examination of the threshold data from each subject to a principal component obtained from both methods showed that the analysis. The first two ...
FREE Sample Here
FREE Sample Here

... 19) Cell membranes are asymmetrical. Which of the following statements is the most likely explanation for the membrane's asymmetrical nature? A) Because the cell membrane forms a border between one cell and another in tightly packed tissues such as epithelium, the membrane must be asymmetrical B) Be ...
How Europe Can Build on its Leading Position
How Europe Can Build on its Leading Position

... 2. Induced pluripotent stem cells derived by reprogramming adult cells are an important breakthrough and a potent new tool for drug discovery, disease research and studying pluripotency, but they are not suitable currently for use in cell therapies. 3. As the “gold standard” of pluripotency, hESC ce ...
Primary endothelial cells isolated from the yolk sac
Primary endothelial cells isolated from the yolk sac

... expansion in vivo. At present it is unclear which cells of the yolk sac microenvironment stimulate the HSC expansion. Several groups have isolated yolk sac and AGM endothelial cell lines and reported that these cell lines stimulate HSC and progenitor cell proliferation and differentiation in vitro.1 ...
Renal Allograft Dysfunction - Beth Israel Deaconess Medical Center
Renal Allograft Dysfunction - Beth Israel Deaconess Medical Center

...  Acute rejection = most common cause of allograft dysfunction during the early posttransplant period  Once occurred in 50-60% renal transplant patients, now estimated to occur in 15-25%  Heralded by diminished urine output and increasing blood pressure  Biopsy: similar to hyperacute rejection wi ...
Lesson Prepared Under MHRD project “National Mission on
Lesson Prepared Under MHRD project “National Mission on

... retained the capacity to divide and form new cells. Meristematic cells are compactly arranged without intercellular spaces. They are present at the apices of root and shoot (apical meristem), between xylem and phloem (lateral meristem) and at leaf and node bases (intercalary meristem) and do not sto ...
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... http://testbankcart.eu/Test-Bank-for-Comprehensive-Radiographic-Pathology-5th-Edi tion-by-Eisenberg ANS: B Staging refers to the extensiveness of a tumor at its primary site and the presence or absence of metastases to lymph nodes and distant organs, such as the liver, lungs, and skeleton. The stagi ...
Associative learning increases adult neurogenesis during a critical
Associative learning increases adult neurogenesis during a critical

... Learning increases the number of immature neurons that survive and mature in the adult hippocampus. One-week-old cells are more likely to survive in response to learning than cells in animals that are exposed to training but do not learn. Because neurogenesis is an ongoing and overlapping process, i ...
DELIVERY OF PROTEIN USING NANOPARTICLE Research Article  A.ROBIN
DELIVERY OF PROTEIN USING NANOPARTICLE Research Article A.ROBIN

... within the cell, and only those drugs can act which make their way into the cell for better efficiency. In order to increase the efficiency, various techniques are adopted to deliver drugs such as Triggered response, in which the drugs are in inactive form within the body and gets activated on sensi ...
GFP-tagging of cell components reveals the dynamics of subcellular
GFP-tagging of cell components reveals the dynamics of subcellular

... 2000; Mano et al., 1999) using GFP-tagging. This approach ß 2003 Blackwell Publishing Ltd ...
Cyclin synthesis and degradation and the embryonic cell cycle
Cyclin synthesis and degradation and the embryonic cell cycle

... cycle is shown (the nucleus is shown solid). ...
Microtubule cortical array organization and plant cell morphogenesis
Microtubule cortical array organization and plant cell morphogenesis

... degree of order. Likewise, when cortical microtubules are depolymerized with drugs then allowed to recover, the array is initially disorganized and gradually regains an ordered appearance, showing that microtubules are not polymerized into their final position [10–12]. Plant cells lack an obvious ce ...
The measurement of exocytosis in plant cells
The measurement of exocytosis in plant cells

... during the life of the cell is incorporated from the secretory vesicle membrane during exocytosis. For each of these processes (polysaccharide, protein and phospholipid delivery) there may be alternative routes, but the available evidence suggests that the exocytotic pathway is of major importance. ...
Localization and characterization of major histocompatibility
Localization and characterization of major histocompatibility

... veins) to a lymphatic system and are thus likely to be involved in lymphocyte trafficking to and from regional lymph nodes. Dendritic cells are normally absent from the brain,11 and it is likely that they are also lacking from the retina. The question, therefore, of how retinal antigenic pepiides ar ...
Expression of Cell Adhesion Molecule E
Expression of Cell Adhesion Molecule E

... yet to be identified. These cadherins are highly related at the amino acid sequence level and probably perform similar functions in different tissues (45). They seem to be particularly important for cell interactions that occur during embryological development, since changes in the expression of cad ...
Polycystin-2 takes different routes to the somatic and ciliary plasma
Polycystin-2 takes different routes to the somatic and ciliary plasma

... appeared after a digest with calf intestinal phosphatase (not depicted), this shows that full-length polycystin-2 becomes phosphorylated soon after it is synthesized. Immunoprecipitated polycystin-2 was also subjected to treatment with PNGase F and endoglycosidase H. Whereas PNGase F removes any N-l ...
Bactericidal activity of the organo
Bactericidal activity of the organo

... Testing the motility and the ability of E. cloacae to form a biofilm Motility assays were performed as described by Marr et al.20 and Overhage et al.21 with some minor modifications. Overnight cultures of E. cloacae were grown on Luria-Bertani (LB) agar plates (Difco). Single colonies of E. cloacae ...
Ectodermal progenitors derived from epiblast
Ectodermal progenitors derived from epiblast

... Present address: Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA ...
Article - Archive ouverte UNIGE
Article - Archive ouverte UNIGE

... dermal papillae after papilla removal, or lower follicle amputation (Oliver, 19666; Jahoda et al. 1991). The finding that cultured rat vibrissa dermal papilla cells (Jahoda and Oliver, 1981) retain their capacity to stimulate hair growth (Jahoda et al. 1984; Home et al. 1986) distinguishes them as a ...
The integrin–actin connection, an eternal love affair
The integrin–actin connection, an eternal love affair

... microtubular network and the directed movement of the cells (Etienne-Manneville and Hall, 2003). Finally, complexity is added by the fact that integrin-associated molecules are multifunctional. Integrin-linked actin binding proteins attach to signalling molecules and function as platforms, which bri ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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