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375 Na+/Ca2+ ANTIPORT IN THE MAMMALIAN HEART
375 Na+/Ca2+ ANTIPORT IN THE MAMMALIAN HEART

... The reasons for the existence of tissue-specific isoforms are uncertain, especially since the kinetic properties of the antiporter are not likely to be affected by alterations in the hydrophilic domain (Matsuoka et al. 1993). However, as discussed below, interactions with the cytoskeleton may be imp ...
Spectrin functions upstream of ankyrin in a spectrin cytoskeleton
Spectrin functions upstream of ankyrin in a spectrin cytoskeleton

... 2004c). From these results, the authors concluded that ankyrin was upstream of spectrin in the spectrin cytoskeleton assembly pathway and that its function was independent of spectrin. However, a study of βIV spectrin knockout mice revealed that βIV spectrin and ankyrin-G are dependent on one anothe ...
Development of Competitive Direct Enzyme
Development of Competitive Direct Enzyme

The role of c-di-GMP signaling in an Aeromonas veronii biovar
The role of c-di-GMP signaling in an Aeromonas veronii biovar

... niches (hospital sewage water, lagoon where duckweed is grown for sewage water purification, fish culture pond where sewage-grown duckweed was used as fish feed and control environmental pond). MT-G was not only detected 2007 Federation of European Microbiological Societies Published by Blackwell Pu ...
Receptor-regulated Dynamic S-Nitrosylation of Endothelial Nitric
Receptor-regulated Dynamic S-Nitrosylation of Endothelial Nitric

Evaluating rRNA as an indicator of microbial activity in
Evaluating rRNA as an indicator of microbial activity in

... breaks down altogether. For example, the relationship between growth rate and rRNA content is not linear or consistent across all measured growth rates. Under balanced growth conditions, Synechococcus and Prochlorococcus strains can have a three-phase relationship between growth and rRNA concentrati ...
Palmitoylethanolamide improves colon
Palmitoylethanolamide improves colon

Regulation of reactive oxygen species homeostasis
Regulation of reactive oxygen species homeostasis

... that AHK3 and ARR2 mediate t-Z- and flg22-induced ROS accumulation in guard cells. We also observed higher levels of ROS in guard cells of IPT3 and ARR2 overexpression lines as compared to Col-0 WT (Figure 3B and Supplemental Figure 3A), which could explain their constitutive closed stomata phenotyp ...
Calcium Oscillations and its Functional Significance in
Calcium Oscillations and its Functional Significance in

... DNA cleavage and contribute to cell killing80, Uncontrolled steady-state rise of [Ca2+]i can also induce Ca2+dependent activation of several genes that characterize many types of acute lethal injury. These genes can be induced within 15 min or less, as in the case of c-fos66 and c-jun, to trigger ad ...
Regulation of Nerve Growth Factor Receptor Gene Expression by
Regulation of Nerve Growth Factor Receptor Gene Expression by

... postnatal days 2-11, and isolated RNA from the superior cervical ganglia (SCG) at postnatal day 12. Northern blot analysis revealed that levels of NGF receptor mRNA increased 5-10-fold relative to total RNA synthesis in NGF-treated versus control SCG (Fig. 1 a). No significant differences were obser ...
Donohoe, B.S., B. - University of Colorado-MCDB
Donohoe, B.S., B. - University of Colorado-MCDB

... is actively producing COPII buds and vesicles for export to the Golgi. To this end, budding COPII vesicles are born within a 40 nm thick scaffold layer that contains Atp115 (Arabidopsis ortholog of p115/Uso1) and appears to have an affinity for the cis-side of the Golgi-encompassing Golgi scaffold/m ...
Evidence for chloroplast control of external Ca -induced
Evidence for chloroplast control of external Ca -induced

... transit peptide were fused to the GFP protein and expressed transiently in Arabidopsis protoplasts. Confocal microscopy revealed co-localization of GFP fluorescence with chlorophyll autofluorescence in chloroplasts (Figure 2a), suggesting that the N-terminal sequence of CAS acts as a chloroplast tra ...
Aalborg Universitet AVP-induced increase in AQP2 and p
Aalborg Universitet AVP-induced increase in AQP2 and p

... Heart failure (HF) is associated with activation of the renin-angiotensin system (RAS) and sustained increased vasopressin (AVP) release from the pituitary gland [1–5]. RAS and AVP have been shown to play a role in the kidneys by taking part in the development of hyponatremia and water retention. Hy ...
Epithelium and mucus
Epithelium and mucus

... bacteria, making it a protective barrier for the enormous bacterial load. The mucus is through this mechanism a part of the innate immunity to keep the homeostasis in colon. The protective function of mucus argues for that defects in the mucus can be a cause of inflammation. In fact, mice with the M ...
Figure 17. Ptc1 expression in Tulp3 mutant limb buds
Figure 17. Ptc1 expression in Tulp3 mutant limb buds

... 4.3 Analysis of limb patterning in Tulp3-/- embryos .................................................................. 93 4.3.1 Loss of Tulp3 affects formation of the ZPA but the AER appears intact ..................... 93 4.3.2 Altered A-P patterning of the autopod in Tulp3 mutant limb buds........ ...
Identification of proteins localized to the contractile vacuole of
Identification of proteins localized to the contractile vacuole of

... bloodstream, acidic phagolysosomes, and host cell cytosol. Thus, the parasites have mechanisms to respond to both hypo-osmotic and hyper-osmotic stresses. The contractile vacuole complex is an osmoregulatory organelle, which controls intracellular water balance by accumulating excess water and expel ...
Penetration of human skin by the cercariae of
Penetration of human skin by the cercariae of

... penetration (Wilson & Lawson, 1980; Wilson, 1987; Wilson et al., 1990; Fusco et al., 1993). Results from these experiments cannot, however, be directly extrapolated to the events that occur on the surface of human skin, because the skin of other mammals is very different from human skin in terms of ...
Superinfection Exclusion by P22 Prophage in Lysogens of Salmonella typhimurium. II. Genetic Evidence for Two Exclusion Systems.
Superinfection Exclusion by P22 Prophage in Lysogens of Salmonella typhimurium. II. Genetic Evidence for Two Exclusion Systems.

... only if the cultures are started by inoculation with colonies taken from solid media; if liquid cultures are serially subcultured, they rapidly accumulate high levels of free phage, many of which are clear-plaque, virulent mutants. These virulent mutants apparently arise during growth of the primary ...
Aalborg Universitet
Aalborg Universitet

... Heart failure (HF) is associated with activation of the renin-angiotensin system (RAS) and sustained increased vasopressin (AVP) release from the pituitary gland [1–5]. RAS and AVP have been shown to play a role in the kidneys by taking part in the development of hyponatremia and water retention. Hy ...
SCL and associated proteins distinguish active from
SCL and associated proteins distinguish active from

PDF Version - Weizmann Institute of Science
PDF Version - Weizmann Institute of Science

... the earliest stages of embryogenesis. Lines with ubiquitous Cre activity will be also useful to process transgenic or targeted mutations by genetic crosses instead of in vitro transfection. This is an advantage, because protracted culture endangers the pluripotentiality of recombinant ES cells and d ...
C-terminal processing of yeast Spt7 occurs in the absence of
C-terminal processing of yeast Spt7 occurs in the absence of

... export machinery [35] suggesting that SAGA may have a broader role in nuclear processes than previously envisioned. Two forms of Spt7 have been described. The full-length protein of 1332 amino acid residues is found within the SAGA complex [2]. The second, a C-terminally truncated form likely arisin ...
Liposomal Formulations for Nucleic Acid Delivery
Liposomal Formulations for Nucleic Acid Delivery

... Liposomes have received attention not only for their utility as model membrane systems, but also for use in drug delivery. Typically, liposomes are used as drug carriers, with the solubilized drug encapsulated in the internal aqueous space formed by the liposomal lamellae. Liposomal drug formulation ...
Meclofenamic acid selectively inhibits FTO demethylation of m A
Meclofenamic acid selectively inhibits FTO demethylation of m A

... make FTO an increasingly interesting target with respect to its functional links to human diseases. Another member of the AlkB family, ALKBH5, was also identified as an m6 A demethylase of mRNA using Fe2+ and cofactor 2OG, which together function to oxidatively remove the methyl group in m6 A-contai ...
The Cultural, Spiritual and Ethical Aspects of Xenotransplantation
The Cultural, Spiritual and Ethical Aspects of Xenotransplantation

... a person who has died as a result of brain damage, for example through a stroke or accident, but it may also be a living donor who has donated, for example, a kidney or some bone marrow. This type of transplant, where the donor and the recipient are both of the same species (in this case human), is ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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