Prions as protein-based genetic elements. - Lindquist Lab
... Strong support for the prion model derives, in part, from biochemical characterization of prion proteins. When crude yeast lysates are fractionated by differential sedimentation, Sup35 and Rnq1 partition differently, depending on whether the cells contain a prion. Sup35 and Rnq1 proteins are mostly ...
... Strong support for the prion model derives, in part, from biochemical characterization of prion proteins. When crude yeast lysates are fractionated by differential sedimentation, Sup35 and Rnq1 partition differently, depending on whether the cells contain a prion. Sup35 and Rnq1 proteins are mostly ...
Compare TIRF Geometries
... living cells. The method shows even the smallest vesicles made by cells, and can image the dynamics of single protein molecules.” [Steyer JA, Almers W. A real-time view of life within 100 nm of the plasma membrane. Nat Rev Mol Cell Biol. 2001, 2(4), 268]. TIRF systems can be implemented in different ...
... living cells. The method shows even the smallest vesicles made by cells, and can image the dynamics of single protein molecules.” [Steyer JA, Almers W. A real-time view of life within 100 nm of the plasma membrane. Nat Rev Mol Cell Biol. 2001, 2(4), 268]. TIRF systems can be implemented in different ...
as a PDF
... was performed, with the aim of understanding how central parameters such as quality, longevity, reliability, and efficacy of commercial bacterial inoculants for agricultural uses can be improved. After exposure of A. brasilense to stress and adverse conditions such as UV-irradiation, desiccation, an ...
... was performed, with the aim of understanding how central parameters such as quality, longevity, reliability, and efficacy of commercial bacterial inoculants for agricultural uses can be improved. After exposure of A. brasilense to stress and adverse conditions such as UV-irradiation, desiccation, an ...
Dentin-pulp complex
... in some cases the pulp chamber can be obliterated. An increase in calcification in the pulp occurs with age. ...
... in some cases the pulp chamber can be obliterated. An increase in calcification in the pulp occurs with age. ...
What is the function of neuroglobin?
... Soon after the discovery of Ngb there was speculation that – in analogy to Mb – it protects neurons from noxious ROS or RNS (Fig. 2B,C). Like any globin, Ngb associates with gaseous ligands other than O2. Under an excess of NO applied in vitro, an NgbFe2+-NO form is established by reductive nitrosyl ...
... Soon after the discovery of Ngb there was speculation that – in analogy to Mb – it protects neurons from noxious ROS or RNS (Fig. 2B,C). Like any globin, Ngb associates with gaseous ligands other than O2. Under an excess of NO applied in vitro, an NgbFe2+-NO form is established by reductive nitrosyl ...
Patterson - Iowa State University
... wild type to determine if the delayed abscission phenotype was heritable and controlled by a single locus. F1 plants of crosses between wild type and dab1-1, dab3-1, dab3-2, or dab3-3 were normal in appearance, whereas approximately 25% of the F2 progeny displayed delayed floral organ abscission. Th ...
... wild type to determine if the delayed abscission phenotype was heritable and controlled by a single locus. F1 plants of crosses between wild type and dab1-1, dab3-1, dab3-2, or dab3-3 were normal in appearance, whereas approximately 25% of the F2 progeny displayed delayed floral organ abscission. Th ...
A heritable switch in carbon source utilization driven
... prion formation are more likely to occur at higher protein concentrations (Chernoff et al. 1993; Ter-Avanesyan et al. 1993; Wickner 1994; Serio et al. 2000; Sondheimer and Lindquist 2000; Derkatch et al. 2001; Uptain and Lindquist 2002; Shorter and Lindquist 2005). The yeast prions also share a dist ...
... prion formation are more likely to occur at higher protein concentrations (Chernoff et al. 1993; Ter-Avanesyan et al. 1993; Wickner 1994; Serio et al. 2000; Sondheimer and Lindquist 2000; Derkatch et al. 2001; Uptain and Lindquist 2002; Shorter and Lindquist 2005). The yeast prions also share a dist ...
Characterization of Vincristine Transport by the Mr 190,000
... It has long been noted that in cells that overexpress P-glycoprotein, the degree of reduced drug accumulation seldom correlates well with the relative resistance of the cells. This is also true of cells that overexpress MRP, and a generally satisfactory explanation for these observations remains elu ...
... It has long been noted that in cells that overexpress P-glycoprotein, the degree of reduced drug accumulation seldom correlates well with the relative resistance of the cells. This is also true of cells that overexpress MRP, and a generally satisfactory explanation for these observations remains elu ...
Identification of a Nuclear Export Signal in the Catalytic Subunit of
... This Article is brought to you for free and open access by the Biology Department at Digital Commons @ Trinity. It has been accepted for inclusion in Biology Faculty Research by an authorized administrator of Digital Commons @ Trinity. For more information, please contact [email protected]. ...
... This Article is brought to you for free and open access by the Biology Department at Digital Commons @ Trinity. It has been accepted for inclusion in Biology Faculty Research by an authorized administrator of Digital Commons @ Trinity. For more information, please contact [email protected]. ...
A direct role for Sox10 in specification of neural crest
... neuron lineage in both mouse and zebrafish (Blader et al., 1997; Perez et al., 1999). Ngn genes are expressed in a subset of NCCs early during migration, but are rapidly downregulated in the nascent DRGs (Greenwood et al., 1999; Perez et al., 1999). DRG neuron specification was not examined in mouse ...
... neuron lineage in both mouse and zebrafish (Blader et al., 1997; Perez et al., 1999). Ngn genes are expressed in a subset of NCCs early during migration, but are rapidly downregulated in the nascent DRGs (Greenwood et al., 1999; Perez et al., 1999). DRG neuron specification was not examined in mouse ...
Neural induction in Xenopus requires early FGF signalling in
... al., 2000). Interestingly, some authors suggested that perhaps FGF signalling in the chick is primarily involved in repressing the expression of Bmp genes (Munoz-Sanjuan and Brivanlou, 2002; Wilson and Edlund, 2001). A similar role has been proposed for early β-catenin activity in the Xenopus ectode ...
... al., 2000). Interestingly, some authors suggested that perhaps FGF signalling in the chick is primarily involved in repressing the expression of Bmp genes (Munoz-Sanjuan and Brivanlou, 2002; Wilson and Edlund, 2001). A similar role has been proposed for early β-catenin activity in the Xenopus ectode ...
Get PDF file - Botanik in Bonn
... environmental signals and requirements. The arrangement and properties of the cytoskeleton are known to be regulated by associated proteins that bind either to monomeric or polymerized cytoskeletal proteins. The number of well studied and characterized cytoskeletonregulating proteins in plant cells ...
... environmental signals and requirements. The arrangement and properties of the cytoskeleton are known to be regulated by associated proteins that bind either to monomeric or polymerized cytoskeletal proteins. The number of well studied and characterized cytoskeletonregulating proteins in plant cells ...
Paraxial mesoderm specifies zebrafish primary motoneuron subtype
... paraxial mesoderm might specify different PMN subtypes. Consistent with this idea, transplantation experiments have shown that environmental signals can specify zebrafish PMN subtypes (Appel et al., 1995; Eisen, 1991). For example, when ...
... paraxial mesoderm might specify different PMN subtypes. Consistent with this idea, transplantation experiments have shown that environmental signals can specify zebrafish PMN subtypes (Appel et al., 1995; Eisen, 1991). For example, when ...
Coordination of microtubule and microfilament dynamics by
... crosslinking by SpireC in the presence of SpireD (data not shown). These new bundling and crosslinking activities raise the possibility that Capu and Spire regulate the onset of ooplasmic streaming by directly mediating coordination of actin assembly and microtubule architecture. Moreover, our find ...
... crosslinking by SpireC in the presence of SpireD (data not shown). These new bundling and crosslinking activities raise the possibility that Capu and Spire regulate the onset of ooplasmic streaming by directly mediating coordination of actin assembly and microtubule architecture. Moreover, our find ...
The Presynaptic Microtubule Cytoskeleton in Physiological and
... use of conventional protocols based on glutaraldehyde fixation and osmium tetroxide post-fixation (Gray and Willis, 1970; Scott and Guillery, 1974). Interestingly, the development of a novel sample preparation protocol, where tissue was incubated with an albumin solution prior to fixation, enabled t ...
... use of conventional protocols based on glutaraldehyde fixation and osmium tetroxide post-fixation (Gray and Willis, 1970; Scott and Guillery, 1974). Interestingly, the development of a novel sample preparation protocol, where tissue was incubated with an albumin solution prior to fixation, enabled t ...
Retinoblastoma Protein Contains a C-terminal - Bio
... polyvinylidene difluoride membrane (2). Western blot analysis of total soluble protein was performed as follows. At 36 h after transfection, U2OS or SAOS2 cells were washed once in cold PBS and scraped into 0.2 ml of EBC lysis buffer (50 mM Tris [pH 8.0], 120 mM NaCl, 0.5% Nonidet P-40). The 150 mg ...
... polyvinylidene difluoride membrane (2). Western blot analysis of total soluble protein was performed as follows. At 36 h after transfection, U2OS or SAOS2 cells were washed once in cold PBS and scraped into 0.2 ml of EBC lysis buffer (50 mM Tris [pH 8.0], 120 mM NaCl, 0.5% Nonidet P-40). The 150 mg ...
How autophagy both activates and inhibits cellular
... affects the microenvironment through the secretion of proinflammatory cytokines, chemokines, growth factors, and proteases, a feature termed the senescence-associated secretory phenotype (SASP). The SASP increases immune surveillance of damaged cells, thus maintaining tissue homeostasis. With age or ...
... affects the microenvironment through the secretion of proinflammatory cytokines, chemokines, growth factors, and proteases, a feature termed the senescence-associated secretory phenotype (SASP). The SASP increases immune surveillance of damaged cells, thus maintaining tissue homeostasis. With age or ...
Activin-mediated mesoderm induction requires FGF
... is to enhance the ability of activin to induce dorsal mesoderm (Green et al., 1992). We therefore asked whether an increased concentration of activin could overcome the ability of the truncated FGF receptor to inhibit the activin-mediated induction of cardiac actin. As shown in Fig. 4B, increasing t ...
... is to enhance the ability of activin to induce dorsal mesoderm (Green et al., 1992). We therefore asked whether an increased concentration of activin could overcome the ability of the truncated FGF receptor to inhibit the activin-mediated induction of cardiac actin. As shown in Fig. 4B, increasing t ...
The Notochord and Floor Plate Guide Growth Cones
... cords. For example, mistakes such as turning ipsilaterally rather than contralaterally or posteriorly rather than anteriorly in the floor plate region by some but not all axons would be difficult to detect. Since it is unclear whether commissural axons have abnormal trajectories at the ventral midli ...
... cords. For example, mistakes such as turning ipsilaterally rather than contralaterally or posteriorly rather than anteriorly in the floor plate region by some but not all axons would be difficult to detect. Since it is unclear whether commissural axons have abnormal trajectories at the ventral midli ...
Endogenous Drp1 Mediates Mitochondrial Autophagy and Protects
... A similar result was obtained in CMs transduced with adenovirus harboring LacZ (Ad-LacZ) (not shown). More than 50% of Ad-shDrp1-transduced CMs exhibited elongated mitochondria after 1 hour of GD. After 4 hours of GD, however, approximately 15% of Ad-shScr- or Ad-LacZ-transduced CMs exhibited foresh ...
... A similar result was obtained in CMs transduced with adenovirus harboring LacZ (Ad-LacZ) (not shown). More than 50% of Ad-shDrp1-transduced CMs exhibited elongated mitochondria after 1 hour of GD. After 4 hours of GD, however, approximately 15% of Ad-shScr- or Ad-LacZ-transduced CMs exhibited foresh ...
Interleukin 12 p40 Production by Barrier Epithelial Cells during
... streptavidin-conjugated alkaline phosphatase complex, and red chromogen. In these experiments, the BAL fluid (without concentration) was subjected to additional analyses of TNF-␣ levels by ELISA (with a sensitivity of 5 pg/ml) and IL-12 by Western blotting. For immunoblotting, cell-free BAL fluid wa ...
... streptavidin-conjugated alkaline phosphatase complex, and red chromogen. In these experiments, the BAL fluid (without concentration) was subjected to additional analyses of TNF-␣ levels by ELISA (with a sensitivity of 5 pg/ml) and IL-12 by Western blotting. For immunoblotting, cell-free BAL fluid wa ...
The Spemann Organizer Signal noggin Binds and
... We then compared the blocking antibody, MAb1A4, with the nonblocking antibodies 2C3 and 5B1 for their ability to immunoprecipitate a noggin-BMP4 complex. A 50-fold molar excess of each antibody was preincubated with noggin protein for 1 hr prior to the addition of 125I-BMP4. All three antibodies pre ...
... We then compared the blocking antibody, MAb1A4, with the nonblocking antibodies 2C3 and 5B1 for their ability to immunoprecipitate a noggin-BMP4 complex. A 50-fold molar excess of each antibody was preincubated with noggin protein for 1 hr prior to the addition of 125I-BMP4. All three antibodies pre ...
Fear-of-intimacy mediated zinc transport controls the
... organogenesis. It impacts on several aspects of myogenesis, including the correct specification and differentiation of founder cells (FCs) and fusion-competent myoblasts (FCMs) in both somatic and visceral mesoderms. Thus, in foi mutants the FCMs segregate but cannot fully differentiate and remain ...
... organogenesis. It impacts on several aspects of myogenesis, including the correct specification and differentiation of founder cells (FCs) and fusion-competent myoblasts (FCMs) in both somatic and visceral mesoderms. Thus, in foi mutants the FCMs segregate but cannot fully differentiate and remain ...
Stimulation Do Not Alter TTP Function Protein Kinase and
... granule localization of the TTP MK2 mutant under basal conditions (13). However, this localization was further enhanced by arsenite stress. This was in contrast to wild-type TTP, the localization of which decreased with arsenite treatment. Finally, we demonstrated that human TTP localizes to the pol ...
... granule localization of the TTP MK2 mutant under basal conditions (13). However, this localization was further enhanced by arsenite stress. This was in contrast to wild-type TTP, the localization of which decreased with arsenite treatment. Finally, we demonstrated that human TTP localizes to the pol ...
Protists - Dillman Biology
... • The word protist is from from the Greek word protistos, which mean “first.” • Knowing this makes it easier to remember that protists were the “first eukaryotes” to evolve. – Fossils indicate that Protists arose about 1.5 mya. ...
... • The word protist is from from the Greek word protistos, which mean “first.” • Knowing this makes it easier to remember that protists were the “first eukaryotes” to evolve. – Fossils indicate that Protists arose about 1.5 mya. ...