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... 393). Because all three channels can be activated by hyposmotic stress, this indicates that they are probably activated sequentially to provide a gradual efflux conduit (147, 168). Studies of the E. coli MscS channel structure demonstrated that it folds as heptamer with three transmembrane helices ( ...
The beneficial role of proteolysis in skeletal muscle growth and
The beneficial role of proteolysis in skeletal muscle growth and

... cleavage [10, 11]. In addition, recent evidence has suggested that the immunoproteasome—so called due to the interferon-γ-induced expression of three alternative proteasome β subunits—also plays a role in myogenesis, with its suppression leading to decreased myoblast differentiation [16]. The necess ...
Chloroplast DNA replication is regulated by the redox state
Chloroplast DNA replication is regulated by the redox state

... whether the replication is coupled with the timing of chloroplast division, even though certain studies have addressed this issue, as described below. Bacteria such as Escherichia coli and Bacillus subtilis possess a single circular chromosome. In these bacteria, the process of DNA replication is t ...
CYTOKINE-MEDIATED REGULATION OF BK VIRUS REPLICATION
CYTOKINE-MEDIATED REGULATION OF BK VIRUS REPLICATION

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PDF

... seen with two distinct morpholinos and the ability to rescue the phenotype indicate that these effects are specific for 3-OST-5, revealing its function in zebrafish LR development. As an important control for functional specificity within the 3-OST family, we knocked down three other family members ...
Molecular assembly of the period-cryptochrome circadian
Molecular assembly of the period-cryptochrome circadian

... DOI: 10.7554/eLife.03674.006 density at the center, hinting at the coordination of a Zn2+ ion at the end of the PER2–CRY2 interface (Figure 3A, Figure 3—figure supplement 1A). Indeed, we were able to validate the identity of the Zn2+ ion by both anomalous dispersion measurements and inductively coup ...
Guanylate cyclase in Dictyostelium discoideum with the topology of
Guanylate cyclase in Dictyostelium discoideum with the topology of

... myosin heavy-chain-specific protein kinase C, which in turn phosphorylate the different myosins, resulting in rearrangements of myosin filaments necessary for movement [14–19]. In order to study the cGMP-dependent pathways in Dictyostelium chemotaxis, we have begun to isolate the genes encoding enzy ...
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PDF

... formation in the mesenchyme. These include a rapid increase in cell proliferation between 12 and 24 h of culture and a shift in the extracellular matrix composition from a mesenchymal to an epithelial type. Based on these observations we have suggested that the responsiveness to transferrin is acqui ...
The Physiological Significance of Mitochondrial Proton Leak in
The Physiological Significance of Mitochondrial Proton Leak in

... compared to those taken from euthyroid controls (Harper & Brand, 1993). Hepatocytes taken from hyperthyroid rats have a 2-fold greater respiration rate compared to euthyroid controls (Harper & Brand, 1993). The proton permeability of mitochondria isolated from liver is 7-fold greater in hyperthyroid ...
Cutting the nonsense: the degradation of PTC containing mRNAs
Cutting the nonsense: the degradation of PTC containing mRNAs

View Full PDF - Essays in Biochemistry
View Full PDF - Essays in Biochemistry

... produced by AdoMetDC. Mammalian AdoMetDC belongs to the group of very few enzymes that uses pyruvate, instead of pyridoxal 5′-phosphate, as a cofactor [18]. The pyruvate is generated by an autocatalytical cleavage of the AdoMetDC protein after synthesis [19]. Mammalian AdoMetDC is produced as a pro- ...
ITO - Université catholique de Louvain
ITO - Université catholique de Louvain

Nuclease activities and cell death processes
Nuclease activities and cell death processes

... recycling of the substrate mycelium proteins of Streptomyces antibioticus ATCC 11891 (Miguelez et al., 1999). The hyphae of S. antibioticus undergo an orderly process of internal cell dismantling, including extensive genome digestion, that resembles the programmed cell death in animal development (M ...
Physical and functional link of the leukemia
Physical and functional link of the leukemia

... Figure 2. PML I isoform specifically interacts with AML1b. (A) Schematic representation of the PML protein and genomic structure of various PML transcripts. The PML protein contains the RBCC motif that consists of the RING finger domain (RING), the 2 B boxes (B1 and B2), and the helical coiled-coil ...
Article Title Authors Author affiliation Correspondence information
Article Title Authors Author affiliation Correspondence information

... autophagy activity in tumors cells correlates with Beclin1 expression. It is believed that Beclin1, an important ATG protein, its yeast homolog, autophagic gene Atg6/Vps30 (vacuolar protein sorting), is a “core” element in membrane formation. Beclin1 recruits and activates the hVps34 protein, they f ...
Role of Hoxa-2 in axon pathfinding and rostral hindbrain patterning
Role of Hoxa-2 in axon pathfinding and rostral hindbrain patterning

... 3694 A. Gavalas and others The Hox genes appear to be good candidates to establish rhombomeric segmental identities, as suggested by the results of the Hoxa-1 and Hoxb-1 knockouts (Lufkin et al., 1991; Chisaka et al., 1992; Mark et al., 1993; Carpenter et al., 1993; Dollé et al., 1993; Goddard et a ...
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PDF

... but become committed shortly before axogenesis (Eisen, 1991), around the time that the alternating pattern of islet2 and islet1 expression is established (Appel et al., 1995). Thus, it was surprising to learn that either Islet1 or Islet2 protein is sufficient to specify both CaP and MiP subtypes, su ...
Mitochondrial Antiviral Signaling Protein Innate Immunity by
Mitochondrial Antiviral Signaling Protein Innate Immunity by

... with TBK1 as well as IKKε leads to activation of IFN regulatory factor (IRF)-3. Coordinated activation of NF-kB and IRF-3 pathways leads to the assembly of a multiprotein enhancer complex that drives expression of IFN-b–and IFN-mediated antiviral immunity (7–11). As a countermeasure, many viruses ha ...
Slow axonal transport and the genesis of neuronal morphology
Slow axonal transport and the genesis of neuronal morphology

... transport model was that it appeared to demand motor proteins with much slower rates than any known class of motor protein. This was particularly worrisome because the motors that move cytoskeletal polymers must be abundant along the length of the axon, and it seemed increasingly unlikely over the y ...
Genetic Models of Mechanotransduction: The
Genetic Models of Mechanotransduction: The

... POPI SYNTICHAKI AND NEKTARIOS TAVERNARAKIS Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Crete, Greece ...
CHAPTER 2 Literature Review I   Bone Homeostasis
CHAPTER 2 Literature Review I Bone Homeostasis

... receptor (RXR), as shown. (Kersten S, Desvergne B, Wahli W. Roles of PPARs in health and disease. Nature 2000;405:421-4). ...
The Young Mouse Heart Is Composed of Myocytes Heterogeneous
The Young Mouse Heart Is Composed of Myocytes Heterogeneous

... straight lines. Cell shortening, maximal rate of contraction (dL/dt), and relaxation (⫹dL/dt) decreased, respectively, 0.32⫾0.058 ␮m, 5.91⫾1.20 ␮m/sec and 5.43⫾0.92 ␮m/sec per 10 000 ␮m3 increase in volume (Figure 3A and 3B). Relaxation was also prolonged in large myocytes but timeto-peak-shortening ...
Yeasts as a model for human diseases
Yeasts as a model for human diseases

... fact that fewer and fewer genes are of unknown function, it serves as a reference genome for the annotation of new ones. For all these reasons, several ‘omics’ approaches were developed using S. cerevisiae. This provided an immense wealth of information that has been used to study more complex organ ...
lecture43.pps
lecture43.pps

... The same mutations typically have major phenotypic consequences in an intact multicellular organism This implies that many of the major roles of cell surface/secreted glycans involve cellcell or extracellular interactions Nuclear/cytosolic glycans may play more cell-intrinsic roles e.g., in signa ...
Increased Agonist Affinity at the
Increased Agonist Affinity at the

... was ⬃2 times above background when mea- Figure 1. Imaging binding and unbinding of dermorphin A594. A, FLAG-MOR in HEK 293 cells were labeled with M1 anti-FLAG suring unbinding, but only 5–10% above back- antibody-conjugated Alexa-488 (M1 A488) to visualize receptors localized on the plasma membrane ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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