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Innate antiviral defense of zebrafish : from signalling to specialized
Innate antiviral defense of zebrafish : from signalling to specialized

... Firstly, I studied a transcription factor that was recently described as a novel participant in IFN signalling. Mammalian promyelocytic leukaemia zinc finger protein (PLZF) could be considered as one of the transcription factors that tune ISG profile specificity to a virus, as it participates in th ...
Ceman, S, O Donnell, WT, Reed, M, Patton, S, Pohl, J and Warren, ST: Phosphorylation regulates translation state of FMRP-associated polyribosomes. Human Molecular Genetics 12:3295-3305 (2003).
Ceman, S, O Donnell, WT, Reed, M, Patton, S, Pohl, J and Warren, ST: Phosphorylation regulates translation state of FMRP-associated polyribosomes. Human Molecular Genetics 12:3295-3305 (2003).

... (compare the upper panels of Fig. 5 where S6 is found in fractions 2–10, to the lower panels which show that following sodium azide treatment, S6 falls back primarily to fractions 2–6). Examination of the sodium azide-treated cells by electron microscopy revealed a loss of polyribosomes upon treatme ...
Organization of cardiac chamber progenitors in the zebrafish blastula
Organization of cardiac chamber progenitors in the zebrafish blastula

... (E-L) Assessment of cardiac contributions at 44 hpf. Lateral views of the heart, anterior towards the left. Labeled myocardial (E,F,I,J) and endocardial (G,H,K,L) progeny are marked with a blue precipitate, and myocardium is counterstained with a pink precipitate (see Materials and methods). Identit ...
Chapter 16—Probes for Endocytosis, Receptors and Ion Channels
Chapter 16—Probes for Endocytosis, Receptors and Ion Channels

... 16.1 Probes for Following Receptor Binding and Phagocytosis The plasma membrane defines the inside and outside of the cell. It not only encloses the cytosol to maintain the intracellular environment but also serves as a formidable barrier to the extracellular environment. Because cells require input ...
A Novel Form of DAP5 Protein Accumulates in Apoptotic Cells as a
A Novel Form of DAP5 Protein Accumulates in Apoptotic Cells as a

RESEALED ERYTHROCYTES: A PROMISING DRUG CARRIER  Review Article   PRAGYA
RESEALED ERYTHROCYTES: A PROMISING DRUG CARRIER  Review Article   PRAGYA

... either  transmission  (TEM)  or  Scanning  electron  microscopy  (SEM).  Other  methods  like  phase  contrast  microscopy  can  also  be  used.  These  techniques  are  done  to  detect  the  morphological  changes  in  the erythrocytes induced by encapsulation methods. 3  Drug Content  Drug conten ...
The N-Terminal Domain of ERK1 Accounts for the Functional
The N-Terminal Domain of ERK1 Accounts for the Functional

Endoplasmic reticulum–mitochondria contacts: function of the junction
Endoplasmic reticulum–mitochondria contacts: function of the junction

... The contact sites between the ER and mitochondria have been measured to be 10–30 nm wide3,4. This distance is close enough to suggest that the two organelles are tethered together by proteins located on the apposing membranes. Ribosomes are also excluded from the ER membrane at contact sites, which ...
TETRASPORE is required for male meiotic
TETRASPORE is required for male meiotic

... condensed chromatin and few organelles, and a larger vegetative cell. The generative cell moves away from the inner wall of the microspore, becoming completely enclosed in the vegetative cytoplasm, and at pollen mitosis II (PMII) divides to form two sperm. In some species, including Arabidopsis, PMI ...
Retinoic acid and chick limb bud development
Retinoic acid and chick limb bud development

... (Thaller and Eichele, 1990). It appears that the signalling system generated by vitamin A metabolism may be more complex than at first envisaged. An important question is whether retinoic acid (or another retinoid) directly acts as a positional signal. Although a gradient of retinoic acid is more ef ...
- Smithsonian Tropical Research Institute
- Smithsonian Tropical Research Institute

... is partly controlled in the retina by the photoreceptor transduction cascade but higher circuits that sum photons for even longer periods of time are also possible. A disadvantage of making vision slower, however, is that the internal spatial details of quickly moving objects cannot be perceived (Sr ...
TETRASPORE is required for male meiotic
TETRASPORE is required for male meiotic

... through the style and into the ovary. The two sperm are transported along the pollen tube into the embryo sac, where one fertilizes the haploid egg to produce the embryo and the other fuses with the diploid central cell to form the nutritive endosperm (Maheshwari, 1950; Knox, 1984). Thus, from the t ...
- Journal of Clinical Investigation
- Journal of Clinical Investigation

... activation was not due to an abnormality in the membrane fraction, as the CGD membranes were capable of generating normal rates of superoxide when activated in the presence of control cytosol. Instead, there was a marked abnormality in cytosol factor activity. Control membranes activated in the pres ...
Cellular functions of the BRCA tumour
Cellular functions of the BRCA tumour

... to the loss of terminal bases that may be removed in order for ligation to occur efficiently. Also, NHEJ does not rely on extensive sequence recognition for repair to proceed so has the capacity to ligate DNA ends from non-homologous chromosomes. This type of aberrant repair may result in acentric o ...
Deep mutational scanning reveals tail anchor
Deep mutational scanning reveals tail anchor

... However, how proteins are targeted to mitochondria by TAs is not understood (Lee et ...
Anopheles  gambiae Drosophila melanogaster Bo Lindberg
Anopheles gambiae Drosophila melanogaster Bo Lindberg

... anophelis, a predominant gut symbiont of An. gambiae recently described in our lab and subsequently found in another laboratory strain of the mosquito. The genome data were then annotated in Paper IV to gain insights into the symbiotic characteristics of the bacterium, as well as the genetic backgro ...
Identification and localization of the multiple bacterial
Identification and localization of the multiple bacterial

... The hindgut of wood-feeding lower termites is densely colonized by a multitude of symbiotic micro-organisms. While it is well established that the eukaryotic flagellates play a major role in the degradation of lignocellulose, much less is known about the identity and function of the prokaryotic symb ...
A three-stage model of Golgi structure and function
A three-stage model of Golgi structure and function

... for the cisternal structure of the Golgi is that this organelle serves as a ‘‘delay timer’’ (Glick and Malhotra 1998). Biosynthetic cargo proteins take some time to traverse the Golgi, and this delay provides an opportunity for resident ER proteins to be retrieved and for cargo proteins to be fully ...
Nucleolar localization of early tRNA processing
Nucleolar localization of early tRNA processing

... et al. 1991) and intron removal in turn preceding exit from nucleus to cytoplasm. In addition, multiple nucleotide modifications occur in the nucleus (Etcheverry et al. 1979; Hopper and Martin 1992). Although the order of processing events is not uniform for all pre-tRNAs and is not absolutely oblig ...
Genetic and Molecular Mechanisms Controlling Reactive
Genetic and Molecular Mechanisms Controlling Reactive

... and signalling of systemic acquired acclimation (SAA), in response to environmentally induced oxidative stress in Arabidopsis are characterized. We used the lesion simulating disease1 (lsd1) mutant as a genetic model system that is deregulated in light acclimation and programmed cell death (PCD). In ...
Nonsense-mediated mRNA decay - Case Western Reserve University
Nonsense-mediated mRNA decay - Case Western Reserve University

... in understanding substrate recognition and in characterizing the protein players required for NMD. However, an increased comprehension of the molecular interactions between the Upf proteins, translational termination and initiation factors, and the decay machinery is needed to reveal the actual mole ...
Definition of a p53 transactivation function-deficient mutant
Definition of a p53 transactivation function-deficient mutant

... suppressor activity is underscored by the fact that the vast majority of p53 mutations derived from tumors map within the sequence-speci®c DNA binding domain (Hollstein et al., 1991; Pavletich et al., 1993; Cho et al., 1994). Growth arrest by p53 seems clearly due to SST of several p53 target genes, ...
Invariant computations in local cortical networks with balanced
Invariant computations in local cortical networks with balanced

... the OSI of the local input region (Fig. 3d), suggesting a relationship between the tuning of synaptic inputs and the orientation representation in the local circuit across a cortical distance of only a few hundred microns. A similar relationship exists for gi and ge (see Fig. 5g,h), and indeed betwe ...
Oxygen Limitation Suppresses Reactive Oxygen Species Formation
Oxygen Limitation Suppresses Reactive Oxygen Species Formation

... difference in size or morphology was evident between treated or untreated samples at 60 minutes and 120 minutes after initiation of treatment. Plate counts were not performed since plating step exposes the bacteria to air where the presence of oxygen could induce hydroxyl radical-mediated killing, a ...
The recovery and purification of fermentation products
The recovery and purification of fermentation products

... The extraction and purification of fermentation products may be difficult and costly. Ideally, one is trying to obtain a high-quality product as quickly as possible at an efficient recovery rate using minimum plant investment operated at minimal costs. Unfortunately, recovery costs of microbial prod ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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