Early developmental specification of the thyroid gland depends on
... Zebrafish hands off mutant embryos have defective thyroid development In zebrafish larvae, the thyroid gland can be visualised using an antibody detecting thyroid hormone (T4) at the apical membrane of follicles from approximately 60 hours post fertilisation (hpf). We found that the hands off mutant ...
... Zebrafish hands off mutant embryos have defective thyroid development In zebrafish larvae, the thyroid gland can be visualised using an antibody detecting thyroid hormone (T4) at the apical membrane of follicles from approximately 60 hours post fertilisation (hpf). We found that the hands off mutant ...
lecture43.pps
... The same mutations typically have major phenotypic consequences in an intact multicellular organism This implies that many of the major roles of cell surface/secreted glycans involve cellcell or extracellular interactions Nuclear/cytosolic glycans may play more cell-intrinsic roles e.g., in signa ...
... The same mutations typically have major phenotypic consequences in an intact multicellular organism This implies that many of the major roles of cell surface/secreted glycans involve cellcell or extracellular interactions Nuclear/cytosolic glycans may play more cell-intrinsic roles e.g., in signa ...
Chediak-Higashi–Like Granules in Acute
... powerful tool for better understanding of happenings inside cells, and showing different processes affecting blood and bone marrow cells. Two years ago Blood honored my devotion to this morphology by publishing one picture of a cytoplasmic bridge between basophilic normoblasts. (Delayed Cytokinesis ...
... powerful tool for better understanding of happenings inside cells, and showing different processes affecting blood and bone marrow cells. Two years ago Blood honored my devotion to this morphology by publishing one picture of a cytoplasmic bridge between basophilic normoblasts. (Delayed Cytokinesis ...
Vibrio cholerae 01 Can Assume a Chlorine
... been accepted (although without overwhelming data) that rough strains have decreased virulence [14, 15], and there has been a tendency to assume that virulence was confined to smooth V. cholerae variants. In prior studies at the Center for Vaccine Development, great care has always been taken to sel ...
... been accepted (although without overwhelming data) that rough strains have decreased virulence [14, 15], and there has been a tendency to assume that virulence was confined to smooth V. cholerae variants. In prior studies at the Center for Vaccine Development, great care has always been taken to sel ...
Distinct patterns of collagen gene expression are seen in normal
... strains (A1 and A2) from atrophic scars exhibited an expression pattern similar to that of normal fibroblasts (Table 5). In contrast, cells from hypertrophic scars showed a steady increase in mRNA expression of proa1(I) and proa1(III) collagens. In keloid fibroblasts, mRNA levels reached a peak at 2 ...
... strains (A1 and A2) from atrophic scars exhibited an expression pattern similar to that of normal fibroblasts (Table 5). In contrast, cells from hypertrophic scars showed a steady increase in mRNA expression of proa1(I) and proa1(III) collagens. In keloid fibroblasts, mRNA levels reached a peak at 2 ...
Chen Tenwek 2015 Bullous Keratopathy
... George R et al. Comparison of Endothelial cell loss and surgically induced astigmatism following conventional extracapsular cataract surgery, manual small-incision surgery and phacoemulsification Gogate P et al. Comparison of endothelial cell loss after cataract surgery: phacoemulsification versus m ...
... George R et al. Comparison of Endothelial cell loss and surgically induced astigmatism following conventional extracapsular cataract surgery, manual small-incision surgery and phacoemulsification Gogate P et al. Comparison of endothelial cell loss after cataract surgery: phacoemulsification versus m ...
Role of Sodium Channel Deglycosylation in the Genesis of Cardiac
... samples were loaded onto 5– 6% discontinuous gels and resolved with SDS-polyacrylamide gel electrophoresis. Equal amounts of MLP"/" and WT proteins were loaded in multiple lanes. Two control and two MLP"/" mice were used in these experiments. Proteins were transferred to polyvinylidene difluoride-pl ...
... samples were loaded onto 5– 6% discontinuous gels and resolved with SDS-polyacrylamide gel electrophoresis. Equal amounts of MLP"/" and WT proteins were loaded in multiple lanes. Two control and two MLP"/" mice were used in these experiments. Proteins were transferred to polyvinylidene difluoride-pl ...
Hemoglobin Receptor in Leishmania Is a Hexokinase Located in the
... 0.72 mM NADP, and 2 units/ml Leuconostoc mesenteroides glucose-6phosphate dehydrogenase (type XXIV from L. mesenteroides; Sigma). The reaction was initiated by the addition of 10 g of cell extract or recombinant HbR, and the initial velocity of NADP reduction (⑀NADPH ⫽ 6.22 ⫻ 106 cm⫺2) was measured ...
... 0.72 mM NADP, and 2 units/ml Leuconostoc mesenteroides glucose-6phosphate dehydrogenase (type XXIV from L. mesenteroides; Sigma). The reaction was initiated by the addition of 10 g of cell extract or recombinant HbR, and the initial velocity of NADP reduction (⑀NADPH ⫽ 6.22 ⫻ 106 cm⫺2) was measured ...
Multiple Wnts and Frizzled Receptors Regulate Anteriorly Directed
... Wnts and Frizzleds Function in A/P Guidance of the AVM and PVM Mechanosensory Neurons In addition to neuronal cell migrations, we also examined guidance of the processes of two mechanosensory neurons, AVM and PVM. A single process from each neuron extends ventrally, enters the ventral nerve cord, an ...
... Wnts and Frizzleds Function in A/P Guidance of the AVM and PVM Mechanosensory Neurons In addition to neuronal cell migrations, we also examined guidance of the processes of two mechanosensory neurons, AVM and PVM. A single process from each neuron extends ventrally, enters the ventral nerve cord, an ...
The Role of Cyclin B in Meiosis I
... thought to interact with the yeast homolog of the invertebrate cyclins (Booher and Beach, 1987, 1988; Solomon et al., 1988; Goebl and Byers, 1988; Reed et al., 1988). Unfortunately, a direct immunochemical test of the idea that cyclins are components of highly purified, active MPF is precluded at th ...
... thought to interact with the yeast homolog of the invertebrate cyclins (Booher and Beach, 1987, 1988; Solomon et al., 1988; Goebl and Byers, 1988; Reed et al., 1988). Unfortunately, a direct immunochemical test of the idea that cyclins are components of highly purified, active MPF is precluded at th ...
b-Arrestin1 and Distinct CXCR4 Structures Are Required for Stromal
... the Mayo Clinic Robert D. and Patricia E. Kern Predoctoral Fellowship for Neuroscience Research (to I.C.C.). dx.doi.org/10.1124/mol.113.089714. s This article has supplemental material available at molpharm.aspetjournals. org. ...
... the Mayo Clinic Robert D. and Patricia E. Kern Predoctoral Fellowship for Neuroscience Research (to I.C.C.). dx.doi.org/10.1124/mol.113.089714. s This article has supplemental material available at molpharm.aspetjournals. org. ...
QNQKE Targeting Motif for the SMN-Gemin Multiprotein Complex in Neurons *
... motor neurons in vivo, but the significance of this peripheral cytoplasmic localization has been largely controversial. Insofar as SMN is involved in snRNP assembly, why would a neuron require this activity to occur so far away from the cell soma? We have applied techniques of fluorescence microscopy ...
... motor neurons in vivo, but the significance of this peripheral cytoplasmic localization has been largely controversial. Insofar as SMN is involved in snRNP assembly, why would a neuron require this activity to occur so far away from the cell soma? We have applied techniques of fluorescence microscopy ...
Saccharomyces cerevisiae
... into P-glucan in 1 min; specific activity was calculated per mg protein. When analysis of residual substrate and by-products was intended, incubation mixtures were treated with 2 vol. ethanol and insoluble glucan was recovered by centrifugation. Supernatants were subjected to paper chromatography an ...
... into P-glucan in 1 min; specific activity was calculated per mg protein. When analysis of residual substrate and by-products was intended, incubation mixtures were treated with 2 vol. ethanol and insoluble glucan was recovered by centrifugation. Supernatants were subjected to paper chromatography an ...
Vesicle-mediated Protein Transport: Regulatory
... Vps34p. In addition, characterization of a temperaturesensitive allele of VPS34 demonstrates that inactivation of Vps34p leads to the immediate missorting of soluble vacuolar proteins (e.g., carboxypeptidase Y) without an apparent defect in the sorting of the vacuolar membrane protein alkaline phosp ...
... Vps34p. In addition, characterization of a temperaturesensitive allele of VPS34 demonstrates that inactivation of Vps34p leads to the immediate missorting of soluble vacuolar proteins (e.g., carboxypeptidase Y) without an apparent defect in the sorting of the vacuolar membrane protein alkaline phosp ...
Bugging the cell wall of bacteria
... elongation and sporulation, respectively [19]. It would therefore be logical that these enzymes are involved in Lipid II translocation during the respective stages of cell growth. At the exterior of the membrane, Lipid II is utilized as the substrate for polymerization reactions resulting in the for ...
... elongation and sporulation, respectively [19]. It would therefore be logical that these enzymes are involved in Lipid II translocation during the respective stages of cell growth. At the exterior of the membrane, Lipid II is utilized as the substrate for polymerization reactions resulting in the for ...
Effects of Ammonia and Lactate on Hybridoma Growth, Metabolism
... all the cultures and accelerated sharply during the decline phase (Fig. 1B). Initially, the drop in viability for batches containing 3.75 mM ammonia was more rapid, but with time these cultures actually displayed higher viabilities. About 75% of the glucose was consumed, producing primarily lactate ...
... all the cultures and accelerated sharply during the decline phase (Fig. 1B). Initially, the drop in viability for batches containing 3.75 mM ammonia was more rapid, but with time these cultures actually displayed higher viabilities. About 75% of the glucose was consumed, producing primarily lactate ...
PDF - The Kaeberlein Lab
... effect of more than 50 mutations and interventions on RLS in the long-lived strain, BY4742. The S288C strain background, from which BY4742 is derived, has not been used extensively in yeast aging analysis. It was chosen: (1) because nearly all non-essential yeast gene deletions have already been cre ...
... effect of more than 50 mutations and interventions on RLS in the long-lived strain, BY4742. The S288C strain background, from which BY4742 is derived, has not been used extensively in yeast aging analysis. It was chosen: (1) because nearly all non-essential yeast gene deletions have already been cre ...
Long-Distance Axonal Regeneration in the Transected Adult Rat
... times. They were then incubated with supernatants of cultured 192 hybridoma cells containing p-75 NGFR at 1:1 dilution in PBS with 5% bovine serum albumin (BSA) for 12 hr at 4°C. After washing with PBS, the dishes were incubated with PBS –5% BSA for 4 hr at room temperature. After dishes were washed ...
... times. They were then incubated with supernatants of cultured 192 hybridoma cells containing p-75 NGFR at 1:1 dilution in PBS with 5% bovine serum albumin (BSA) for 12 hr at 4°C. After washing with PBS, the dishes were incubated with PBS –5% BSA for 4 hr at room temperature. After dishes were washed ...
Molecular and Metabolic Imaging of Atherosclerosis*
... measure plaque metabolism, and numerous studies are currently under way to assess its role in predicting clinical events and monitoring changes in plaque composition (21,22). Despite its promise, it is an expensive and invasive technique that is unlikely to gain widespread clinical application. In s ...
... measure plaque metabolism, and numerous studies are currently under way to assess its role in predicting clinical events and monitoring changes in plaque composition (21,22). Despite its promise, it is an expensive and invasive technique that is unlikely to gain widespread clinical application. In s ...
Auxin and other signals on the move in plants
... Short-range transport. Membranes of plant cells are not in direct contact with each other because of the presence of a cellulose-based cell wall. Thus, molecules have three possibilities for short-range movement: (i) transport inside the cell wall, without entering the cell (apoplastic transport); ( ...
... Short-range transport. Membranes of plant cells are not in direct contact with each other because of the presence of a cellulose-based cell wall. Thus, molecules have three possibilities for short-range movement: (i) transport inside the cell wall, without entering the cell (apoplastic transport); ( ...
Diversification of epithelial adherens junctions with independent
... classic cadherins (referred to simply as cadherins), whose extracellular domain organization displays marked variety among species examined so far. In this study, we attempted to reconstruct the evolution of cadherin by analyzing new data from several arthropods (two insects, one noninsect hexapod, ...
... classic cadherins (referred to simply as cadherins), whose extracellular domain organization displays marked variety among species examined so far. In this study, we attempted to reconstruct the evolution of cadherin by analyzing new data from several arthropods (two insects, one noninsect hexapod, ...
Glucose transporter gene expression in early mouse
... domains. There is considerable sequence conservation in these domains between the five members of the GLUT family characterized so far. It is probable that the transmembrane domains are necessary to form the channel through which glucose is carried. The specific mechanism of glucose transport and th ...
... domains. There is considerable sequence conservation in these domains between the five members of the GLUT family characterized so far. It is probable that the transmembrane domains are necessary to form the channel through which glucose is carried. The specific mechanism of glucose transport and th ...
The transcription factor FoxB mediates temporal
... inductive events depends on the presence of not only an inductive signal but also on cellular competence, i.e. the ability of cells to receive and respond to inductive signals. These properties are temporally and spatially regulated in a coordinated manner, ensuring that tissues are induced at the r ...
... inductive events depends on the presence of not only an inductive signal but also on cellular competence, i.e. the ability of cells to receive and respond to inductive signals. These properties are temporally and spatially regulated in a coordinated manner, ensuring that tissues are induced at the r ...
Effects of cytochalasin B on meiosis and development of fertilized
... In normal development a polar lobe develops at the vegetal pole of the egg, long before the indication of the first cleavage furrow. During cytochalasin B treatment we do not observe any attempt of the egg to produce such a formation. This holds true not only for eggs treated from the onset of meios ...
... In normal development a polar lobe develops at the vegetal pole of the egg, long before the indication of the first cleavage furrow. During cytochalasin B treatment we do not observe any attempt of the egg to produce such a formation. This holds true not only for eggs treated from the onset of meios ...