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Bioreactor landfills: experimental and field results
Bioreactor landfills: experimental and field results

... distribution of the recirculated leachate. The nominal size of the gravel used for both filter layers range from 19 to 38 mm. Finally, the bioreactor cell covers were placed on top of each cell, and the whole unit was sealed using construction type sealant. The protruding ends of the gas collection w ...
Production and consumption of nitrous oxide in nitrate
Production and consumption of nitrous oxide in nitrate

... some denitrifying organisms lack N2O reductase activity and release N2O as an end product. Recently, nitrate- (or nitrite-) ammonifying cells have also been reported to release N2O (Stremińska et al., 2012; Giles et al., 2012). In bacterial denitrification and in denitrifying nitrifiers, NO is redu ...
Nucleic Acid Carriers Based on Precise Polymer
Nucleic Acid Carriers Based on Precise Polymer

... weight and isomers, which significantly influences physicochemical properties, biological efficiency, and cytotoxicity.2528 Moreover, random attachment of one or even several different functional domains results in heterogeneous conjugates that might be useful in experimental approaches, but are rather ...
Screening and Identification of Yeasts Antagonistic to Pathogenic
Screening and Identification of Yeasts Antagonistic to Pathogenic

... Microbes have evolved ways of interference competition to gain advantage over their ecological competitors. The use of secreted antagonistic compounds by yeast cells is one of the prominent examples. Although this killer behavior has been thoroughly studied in laboratory yeast strains, our knowledge ...
Gain and Loss of Photosynthetic Membranes during
Gain and Loss of Photosynthetic Membranes during

... chloroplasts are already found. A developmental gradient was thus predicted to exist between these two regions of the shoot apex. In this study, we applied different microscopic techniques to characterize the maturation state of the thylakoid membrane in plastids dispersed throughout the shoot apex ...
Applications of Microscopy in Bacteriology
Applications of Microscopy in Bacteriology

... bacteriology which includes a large group of typically unicellular prokaryotic and eukaryotic bacteria widely distributed in air, water, soil etc. and therefore bacteriology requires many varied techniques and instruments. Microscopy is a great achievement in this respect [2] [3]. Microscopy promise ...
Mini Review An Overview on Bacterial Motility Detection
Mini Review An Overview on Bacterial Motility Detection

... preparation could be the most used method in some motility determination cases unless the use of depression slide. Basically, hanging drop is prepared by applying a little vaseline or stopcock grease to the four corners of the coverslip and transferring a loopful of fresh liquid culture to the centr ...
INTRODUCTION the dorsal spinal cord (Muroyama et al., 2002) and for... Wnts are signaling molecules regulating different developmental
INTRODUCTION the dorsal spinal cord (Muroyama et al., 2002) and for... Wnts are signaling molecules regulating different developmental

... Lipofectamine Plus reagent (Invitrogen) with the expression vector for the full-length cDNA of mouse Lef1-HA, ␤-catenin (gifts of Dr Grosschedl, Max Planck Institute of Immunology, Germany), Tcf3 (gift of Dr Piccolo, University of Padua, Italy), Gli1 and human GLI3 (gifts of Dr Sasaki, Center for De ...
Deep Insight Section Mechanisms and regulation of autophagy in mammalian cells
Deep Insight Section Mechanisms and regulation of autophagy in mammalian cells

... organelles. There are two major systems in eukaryotic cells that degrade cellular components: the ubiquitin proteasome system (UPS) and the lysosome. The UPS only degrades proteins, mainly short-lived proteins that have to be tagged by ubiquitin to be recognized by the proteasome (Ciechanover et al. ...
Placental Histopathologic Changes Associated with Subclinical
Placental Histopathologic Changes Associated with Subclinical

... the maternal side of the placenta, and the placental blood that pooled in these areas was collected by syringe aspiration into a tube containing EDTA. The tissue sections were then used to make impression smears. A full-thickness section of the placenta was taken approximately one-third of the dista ...
Novel Insights into Vacuole-mediated Control of Plant Growth and
Novel Insights into Vacuole-mediated Control of Plant Growth and

... References ...
Sharp, K. A microscopic investigation of purple sulfur bacterial
Sharp, K. A microscopic investigation of purple sulfur bacterial

... pool. It was my original hypothesis that SRBs produced sulfide and presented a “nucleating center” for the PSBs to attach and start building an aggregate. However, the majority of the cells in the berries seem to be accounted for with the microscopy. The clusters are PSBs, and the small unicellular ...
The ATP synthase is involved in generating mitochondrial cristae
The ATP synthase is involved in generating mitochondrial cristae

... and DTIM11 mutant cells grew using lactate as carbon source either at 28 or 37°C, thus indicating that they were able to generate ATP via oxidative phosphorylation. However, they had generation times longer than that of the wild-type strain. Indeed, 40% of DATP20 and DTIM11 mutant cells spontaneousl ...
Early and late endosomal compartments of Entamoeba histolytica
Early and late endosomal compartments of Entamoeba histolytica

... inactive (GDP-bound) states; this switching is controlled, in part, by the transient association of the Rab protein with regulatory proteins [16]. By alternating between membrane-bound and cytosolic forms and by binding and hydrolyzing GTP, Rab GTPases can function as “molecular switches” to regulat ...
ICAM-1 - The Journal of Cell Biology
ICAM-1 - The Journal of Cell Biology

... A mixture of two mouse mAb, RR1/1 (46) and R6.5 (49) or CL203 (31), was used to detect ICAM-1. LFA-1 was stained with a combination of three mAb N217, N225, and N226 obtained from IVth Leukocyte Typing Workshop (28). CD43 was detected with mAb N23, CD44 with mAb N85, and CD45 with mAb N88 all obtain ...
Peer-reviewed Article PDF - e
Peer-reviewed Article PDF - e

... The sarco/endoplasmic Ca2+ATPase (SERCA) belong to a family of P-type ion pumps [23]. The growing family of SERCA isoforms is coded by 3 ATP2A1-3 genes located on 3 different chromosomes, encoding for SERCA1, SERCA2 and SERCA3 isoforms respectively; further diversity is generated by alternative spli ...
The chick somitogenesis oscillator is arrested before all paraxial
The chick somitogenesis oscillator is arrested before all paraxial

... streak (or tail bud) and come to lie alongside the notochord. Groups of cells at the most rostral end of each PSM bud off with a remarkable periodicity and synchronisation as an epithelial sphere of cells to form the new somite. It is widely accepted that this process is controlled by a molecular os ...
Chapter 4 - Neurasync
Chapter 4 - Neurasync

... situation for macroautophagy, since starvation also fails to promote membraneassociated LC3-II formation in GFP-LC3 mice (25). This of course does not negate the importance of macroautophagy in the nervous system, which is now widely appreciated. In fact, we have found Lamp2a levels in the brain, a ...
Receptor Regulation of the Volume-Sensitive Efflux of Taurine and
Receptor Regulation of the Volume-Sensitive Efflux of Taurine and

... comes from studies in which RVD, volume-sensitive Cl⫺ current, and organic osmolyte release can all be blocked by broad-spectrum anion channel inhibitors, such as DDF or NPPB, and by a highly selective agent, DCPIB (Decher et al., 2001; Abdullaev et al., 2006). Similarities in the pharmacological in ...
Effects of nitric oxide on red blood cell deformability
Effects of nitric oxide on red blood cell deformability

... study was designed to further explore this possibility. Human RBCs in autologous plasma were incubated for 1 h with ...
β-catenin controls differentiation of the retinal pigment epithelium in
β-catenin controls differentiation of the retinal pigment epithelium in

... formation. Subsequent to RPE specification, a period of differentiation and maturation follows, resulting in dramatic morphological, structural and functional changes (Rizzolo, 2007; Strauss, 2005). Interestingly, the RPE fate is reversible for several days following the initial activation of differ ...
Receptor Regulation of the Volume-Sensitive Efflux of Taurine and
Receptor Regulation of the Volume-Sensitive Efflux of Taurine and

... comes from studies in which RVD, volume-sensitive Cl⫺ current, and organic osmolyte release can all be blocked by broad-spectrum anion channel inhibitors, such as DDF or NPPB, and by a highly selective agent, DCPIB (Decher et al., 2001; Abdullaev et al., 2006). Similarities in the pharmacological in ...
Reverse migration of neutrophils: Where, when, how and why
Reverse migration of neutrophils: Where, when, how and why

... detailed in Box 1. Specifically, through the use of genetically modified mice exhibiting RFPcherrypericytes (under the control of the αSMA promoter) and GFP-neutrophils (LysM-GFP-ki mice), realtime imaging has revealed that post TEM, neutrophils exhibit significant crawling along pericyte processes ...
The Crucial Role of Biofilms in Cryptococcus neoformans Survival
The Crucial Role of Biofilms in Cryptococcus neoformans Survival

... and undetected by the host immune system, the cryptococci are able to disseminate and invade different organ systems, having particular affinity for the central nervous system (CNS). Furthermore, C. neoformans has developed the intricate strategy of phagosomal extrusion where the fungus can exit mac ...
Cellular Pathology
Cellular Pathology

... pigment seen in the hepatocytes here is lipochrome (lipofuscin) which accumulates over time in cells (particularly liver and heart) as a result of "wear and tear" with aging. It is of no major consequence, but illustrates the end result of the process of autophagocytosis in which intracellular debri ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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