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Bacillus anthracis produces membrane-derived vesicles containing biologically active toxins
Bacillus anthracis produces membrane-derived vesicles containing biologically active toxins

... Many Gram-negative pathogenic bacterial species, including Pseudomonas aeruginosa, produce vesicles that contain toxins or other virulence factors and, in several cases, vesicles have been proposed to be vehicles for toxin delivery to eukaryotic cells (14– 17). Significantly less is known about the r ...
Enhancers as non-coding RNA transcription units: recent
Enhancers as non-coding RNA transcription units: recent

The Role Of The Planar Cell Polarity Pathway In The Second Heart
The Role Of The Planar Cell Polarity Pathway In The Second Heart

... microcosm, from where all vigor and strength does flow. —William Harvey, De Motu Cordis, 1628 ...
Cornell University,  1989 M.S. Environmental Engineering Stanford University,  1990
Cornell University, 1989 M.S. Environmental Engineering Stanford University, 1990

... Olson for their patience and devotion to my work over these many years. Through them, I have been able to gain many different insights into the world of oceanography, and science in general. Over my final year, I have benefited tremendously from involvements with two other scientists. Dave Karl open ...
Vertebrate limb development--the early stages in chick - ICB-USP
Vertebrate limb development--the early stages in chick - ICB-USP

... (Fgf8 is never expressed). A strikingly similar limb phenotype was observed more recently in the knockout of FGF receptor 2-IIIb [12•], for which FGF10 had previously been suggested to be the major ligand [13]. Mice deficient for this particular receptor isoform show agenesis of limbs but, surprisin ...
Synaptic Mechanisms of Forward Suppression in Rat
Synaptic Mechanisms of Forward Suppression in Rat

... forward suppression (a 66% reduction in the P2/P1 ratio, Figure 3B). Inhibitory duration was similarly increased in all cells held through intraperitoneal injection. On average, the time constant of inhibitory decay increased by 643% ± 165% (n = 4 cells, mean ± SEM). We conclude that the prolonged i ...
Plant Growth Regulators II: Cytokinins, their Analogues and
Plant Growth Regulators II: Cytokinins, their Analogues and

... Chapter 4); amongst which have been found the natural cytokinin zeatin, and 1,3-diphenylurea (11) (Shantz and Steward, 1955). The latter compound, and many other substituted ureas, have cytokinin activity and can promote the growth of dormant buds (Kefford et al., 1966), and induce cell division in ...
Temporal Regulation of RNA Polymerase II by Srb10 and Kin28
Temporal Regulation of RNA Polymerase II by Srb10 and Kin28

... Lu et al., 1992; Serizawa et al., 1992). The Srb10 kinase has been proposed to be a CTD kinase based upon genetic evidence for an interaction with the CTD and evidence that holoenzyme preparations lacking Srb10 activity have substantially reduced CTD kinase activity (Liao et al., 1995). We tested wh ...
The Shwachman-Diamond SBDS protein localizes to the nucleolus
The Shwachman-Diamond SBDS protein localizes to the nucleolus

... the effects of hypomorphic alleles and that complete loss of SBDS function was likely to be lethal.16 This observation provides a possible rationale for the prevalence of this splice site mutation in patients with SDS. In further support of the antibody specificity, the introduction of a wild-type S ...
Engineering microfluidic concentration gradient generators for
Engineering microfluidic concentration gradient generators for

... A microfluidic gradient generator manipulates small volumes of reagents, usually in nanolitres or microlitres, through networks of sub-micron to micrometer sized channels. The advances in microfabrication technology led to the continuous miniaturization of channel geometries, thereby reducing gradie ...
Engineering microfluidic concentration gradient generators for
Engineering microfluidic concentration gradient generators for

... A microfluidic gradient generator manipulates small volumes of reagents, usually in nanolitres or microlitres, through networks of sub-micron to micrometer sized channels. The advances in microfabrication technology led to the continuous miniaturization of channel geometries, thereby reducing gradie ...
Spatiotemporal Patterns of Expression of NGF and the Low
Spatiotemporal Patterns of Expression of NGF and the Low

... ends of the limb persisted throughout limb development, with some of the highest levels of expression occurring at the limb axillary sites, which were not highly innervated. We have also found ~75”” expression at sites of mesenchymal/epithelial interactions in several developing organs that do not a ...
Involvement of the Sieve Element Cytoskeleton in
Involvement of the Sieve Element Cytoskeleton in

... Figure 2. Immunocytochemical visualization of a parietal actin network in SEs of fava bean. For indirect immunolocalization, ultrathin sections were labeled with clone C4 anti-actin (dilution in A–C, 1:100; dilution in D, 1:200) or with buffer (control; E) and treated with 5-nm gold-labeled secondar ...
RA/FGF control Meis in PD limb development
RA/FGF control Meis in PD limb development

... with Meis activation in the apical ectoderm (Fig. 1I,J). To avoid the negative effect upon the AER, we inserted the beads into the distal mesenchyme about 150 µm apart from the AER, a position referred hereafter as subapical. When RA beads were applied subapically in the distal mesenchyme, limb grow ...
The glycocalyx is present as soon as blood flow is initiated and is
The glycocalyx is present as soon as blood flow is initiated and is

... The stage at which both the glycocalyx and the ESL form during vascular development is not known. Many of the proteins that produce the GAGs of the glycocalyx are expressed before the onset of blood flow (Klewer et al., 2006). Lectins are proteins that bind to specific disaccharides in glycosaminoglyc ...
Masking the Pathogen: Evolutionary Strategies of Fungi and Their
Masking the Pathogen: Evolutionary Strategies of Fungi and Their

... influenzae [27,28], and is known to share antigenic determinants with certain pneumococcal PSs [29,30]. In addition, the presence of capsule has been implicated in biofilm formation, which correlates with the ability of capsular PS to bind polystyrene solid supports [31]. The primary structure of th ...
Bradykinin stimulates IL-6 and IL-8 production by human lung
Bradykinin stimulates IL-6 and IL-8 production by human lung

... generated from kininogens by the action of plasma and tissue kallikreins. It has been postulated that the kallikreinkinin system is important in the pathophysiology of acute lung injury, acute (adult) respiratory distress syndrome (ARDS) and multiple organ failure. Experimental endotoxin shock, bact ...
What`s the Difference? Efficient Set Reconciliation without
What`s the Difference? Efficient Set Reconciliation without

... We start with a simple model of set reconciliation. For two sets SA , SB each containing elements from a universe, U = [0, u), we want to compute the set difference, DA−B and DB−A , where DA−B = SA − SB such that for all s ∈ DA−B , s ∈ SA and s∈ / SB . Likewise, DB−A = SB −SA . We say that D = DA−B ...
Subcellular Trafficking of Mammalian Lysosomal Proteins: An
Subcellular Trafficking of Mammalian Lysosomal Proteins: An

... and enrichment level over total proteins in each fraction) differs from those reported for proteins located in other cellular structures led to the discovery of lysosomes ([1], reviewed by Sabatini and Adesnik [2]). Today, proteomic analyses have revealed that the lumen of lysosomes contains approxi ...
Keystone Standards
Keystone Standards

... BIO.B.4. 1. Describe ecological levels of organization in the biosphere. BIO.B.4. 1.1. Describe the levels of ecological organization (i.e., organism, population, community, ecosystem, biome, and biosphere). BIO.B.4. 1.2. Describe characteristic biotic and abiotic components of aquatic and terrestri ...
a cells- secrete glucagon
a cells- secrete glucagon

... cell ultimately leads to membrane depolarization and an influx of extracellular calcium. The resulting increase in intracellular calcium is thought to be one of the primary triggers for exocytosis of insulin-containing secretory granules. ...
Brachyury and SMAD signalling collaboratively
Brachyury and SMAD signalling collaboratively

... Genomic targets of BRA orthologues have previously been identified in zebrafish (Morley et al., 2009) and Xenopus embryos (Gentsch et al., 2013), and in mouse embryoid bodies (Evans et al., 2012; Lolas et al., 2014) using ChIP-chip or ChIP-seq approaches. Recently, BRA target genes were identified i ...
Dopamine transporter: involvement in selective dopaminergic
Dopamine transporter: involvement in selective dopaminergic

... and it is believed to control the intensity and duration of DAergic neurotransmission by setting the concentration of DA in the extracellular space. Depending on cellular conditions, DAT is able to transport DA bi-directionally, but at normal membrane potential and Naþ gradient the inward transport ...
The Panicum mosaic virus-like 3` cap
The Panicum mosaic virus-like 3` cap

... In canonical eukaryotic translation initiation, the recognition of mRNA by the translation machinery is facilitated through the binding of the 5’ m7G cap structure to eukaryotic initiation factor 4E (eIF4E). This recognition is necessary for efficient translation of the message to occur. In contrast ...
The PDZ-GEF Dizzy regulates cell shape of migrating macrophages
The PDZ-GEF Dizzy regulates cell shape of migrating macrophages

... From worms to humans, cell migration is an essential cellular process: during embryonic development cells migrate to specific locations, where they subsequently differentiate to form tissues and organs. Then later in adult organisms, cell migration is implicated in tissue renewal and immune response ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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