Divergent Functional Properties of the Ribosome
... engineered just upstream of the ATG by PCR. This site was then used to clone a 3.2-kb fragment containing SSB into the BamHI site of pRSETB (Invitrogen, Carlsbad, CA), which contains six histidines and an anti-express antibody (Invitrogen) epitope. The gene encoding His-Ssb1 was moved as a 3.4-kb Xb ...
... engineered just upstream of the ATG by PCR. This site was then used to clone a 3.2-kb fragment containing SSB into the BamHI site of pRSETB (Invitrogen, Carlsbad, CA), which contains six histidines and an anti-express antibody (Invitrogen) epitope. The gene encoding His-Ssb1 was moved as a 3.4-kb Xb ...
Specific Aims – Background and Gap, Aim 1 and 2 Schizophrenia is
... Omega. CRISPR-Cas9 will be used to knock-out the most conserved areas of different domains in zebrafish embryos and adult trials, then synaptic pruning is visualized by fluorescence imaging of dendritic proteins in the cortex neurons. Knocked out domains which reduce the amount of pruning in adults ...
... Omega. CRISPR-Cas9 will be used to knock-out the most conserved areas of different domains in zebrafish embryos and adult trials, then synaptic pruning is visualized by fluorescence imaging of dendritic proteins in the cortex neurons. Knocked out domains which reduce the amount of pruning in adults ...
Modulation of the Antigenic Peptide Transporter TAP by
... linear epitope was identified corresponding to the very last five C-terminal amino acid residues of TAP1 ( 744 ADAPE 748 ) (Figure 2(b) and (c)). Here, the binding efficiency was fully preserved when compared to the 15mer peptide. Further mutational analyses demonstrate that the residues D745 and E7 ...
... linear epitope was identified corresponding to the very last five C-terminal amino acid residues of TAP1 ( 744 ADAPE 748 ) (Figure 2(b) and (c)). Here, the binding efficiency was fully preserved when compared to the 15mer peptide. Further mutational analyses demonstrate that the residues D745 and E7 ...
Ribozyme catalysis: not different, just worse
... water molecule to the leaving group oxygen48. In the precursor active site, C75 is positioned near the 2′-hydroxyl nucleophile, suggesting possible general-base function to initiate the reaction by deprotonating the 2′-hydroxyl nucleophile (Fig. 1c). The situation is murkier still for the hairpin ri ...
... water molecule to the leaving group oxygen48. In the precursor active site, C75 is positioned near the 2′-hydroxyl nucleophile, suggesting possible general-base function to initiate the reaction by deprotonating the 2′-hydroxyl nucleophile (Fig. 1c). The situation is murkier still for the hairpin ri ...
This Article Abstract Full Text (PDF) Alert me when this article is cited
... were reported for a number of Wzc homologues (16, 27, 37). In E. coli K-12, five of the six C-terminal tyrosine residues can be phosphorylated in vitro (16). However, it is not clear at this point whether all of the Wzc C-terminal tyrosine residues or one or more specific tyrosine residues are acces ...
... were reported for a number of Wzc homologues (16, 27, 37). In E. coli K-12, five of the six C-terminal tyrosine residues can be phosphorylated in vitro (16). However, it is not clear at this point whether all of the Wzc C-terminal tyrosine residues or one or more specific tyrosine residues are acces ...
Peer-reviewed Article PDF - e
... Muscle Cell: SERCA2a/SERCA2b Switch as a Key Difference between the Contractile and Synthetic ...
... Muscle Cell: SERCA2a/SERCA2b Switch as a Key Difference between the Contractile and Synthetic ...
Microenvironment analysis and identification of magnesium binding
... the electrostatic ®eld (44). We believe that Mg2+ binding sites can be differentiated not only by charge alone, but also by the biochemical and structural properties surrounding the binding site. We used FEATURE to study the differences between site-bound and diffusely bound Mg2+ ions in complex RNA ...
... the electrostatic ®eld (44). We believe that Mg2+ binding sites can be differentiated not only by charge alone, but also by the biochemical and structural properties surrounding the binding site. We used FEATURE to study the differences between site-bound and diffusely bound Mg2+ ions in complex RNA ...
A defined subset of adenylyl cyclases is regulated by bicarbonate ion.
... To define the extent to which class III ACs may be stimulated by HCO3- we have utilized the cyaB1 AC gene of the nitrogen fixing freshwater cyanobacterium Anabaena sp. PCC7120 as a model system. Cyanobacteria are dependent upon the accumulation of intracellular HCO3- for growth but the mechanism by ...
... To define the extent to which class III ACs may be stimulated by HCO3- we have utilized the cyaB1 AC gene of the nitrogen fixing freshwater cyanobacterium Anabaena sp. PCC7120 as a model system. Cyanobacteria are dependent upon the accumulation of intracellular HCO3- for growth but the mechanism by ...
Protein phosphorylation in chloroplasts – a survey of
... could find a significant peptide match to a spectrum while the same spectrum does not produce a significant match at different settings or with other software. By no means should two search strategies result in different significant matches for the same peptide. However, with the up-to-date software ...
... could find a significant peptide match to a spectrum while the same spectrum does not produce a significant match at different settings or with other software. By no means should two search strategies result in different significant matches for the same peptide. However, with the up-to-date software ...
Poster
... regulated proliferation. Its misregulation by APC, a protein utilized in the Wnt pathway involved in marking β-catenin for degradation, is an important event in colon cancer5. Although several binding affinities of β-catenin have been described, several questions remain. In particular, the function ...
... regulated proliferation. Its misregulation by APC, a protein utilized in the Wnt pathway involved in marking β-catenin for degradation, is an important event in colon cancer5. Although several binding affinities of β-catenin have been described, several questions remain. In particular, the function ...
Antibody domain exchange is an immunological solution to
... shielded. • Carbohydrate is made by host (not virus enzymes) hence may be “self” (though the dense cluster of oligomannose residues has not been described on mammalian glycoproteins). ...
... shielded. • Carbohydrate is made by host (not virus enzymes) hence may be “self” (though the dense cluster of oligomannose residues has not been described on mammalian glycoproteins). ...
Identification and Structural Characterization of the ATP/ADP
... Hsp70/DnaK. Although the apparent involvement of ATP in p23 binding remains unexplained, it has become widely accepted that unlike Hsp70/DnaK and Hsp60/ GroEL, Hsp90 does not bind ATP. Although the structure of the intact Hsp90 molecule has not yet been determined, crystal structures of an amino-ter ...
... Hsp70/DnaK. Although the apparent involvement of ATP in p23 binding remains unexplained, it has become widely accepted that unlike Hsp70/DnaK and Hsp60/ GroEL, Hsp90 does not bind ATP. Although the structure of the intact Hsp90 molecule has not yet been determined, crystal structures of an amino-ter ...
Structural insights into glycoside hydrolase family 32 and 68
... Structural insights into GH32 and GH68 | 729 (with an extra C-terminal domain: see next section) belong to GH32. Invertases (EC 3.2.1.26) split sucrose into glucose and fructose by cleavage of the a1-b2-glycosidic bond. Plant invertases are found in two separate GH families: acid invertases (cell w ...
... Structural insights into GH32 and GH68 | 729 (with an extra C-terminal domain: see next section) belong to GH32. Invertases (EC 3.2.1.26) split sucrose into glucose and fructose by cleavage of the a1-b2-glycosidic bond. Plant invertases are found in two separate GH families: acid invertases (cell w ...
Calretinin
... • Ca2+ binding protein present in the neurons of most vertebrates • Functions as a Ca2+ buffer • 6 EF hands but only four or five of appear to be functional and suitable for Ca2+ binding/buffering • The EF-hand Ca2+ binding site consists of an α-helix, a loop, and another αhelix ...
... • Ca2+ binding protein present in the neurons of most vertebrates • Functions as a Ca2+ buffer • 6 EF hands but only four or five of appear to be functional and suitable for Ca2+ binding/buffering • The EF-hand Ca2+ binding site consists of an α-helix, a loop, and another αhelix ...
Identify domains in LPHN3 crucial to synaptic density.
... Latrophilin Latrophilin Latrophilin Peptidase ...
... Latrophilin Latrophilin Latrophilin Peptidase ...
Computational and genetic evidence that different structural
... might adopt either conformation in vivo. Although folding only two of the eight predicted TMHs presents an underconstrained problem, the small energy difference between the “up” and “down” conformations suggests that there may be a binary state for this region. To validate our ab initio method, we g ...
... might adopt either conformation in vivo. Although folding only two of the eight predicted TMHs presents an underconstrained problem, the small energy difference between the “up” and “down” conformations suggests that there may be a binary state for this region. To validate our ab initio method, we g ...
PDF - Bezanilla Lab
... used a combination of loss-of-function and complementation analyses, together with biochemical characterization, to dissect the role and molecular functions of formins in plants. Results Silencing of All Class I Formins (For1) Affects Plant Size, but Not Polarized Growth. Moss For1 form a monophylet ...
... used a combination of loss-of-function and complementation analyses, together with biochemical characterization, to dissect the role and molecular functions of formins in plants. Results Silencing of All Class I Formins (For1) Affects Plant Size, but Not Polarized Growth. Moss For1 form a monophylet ...
The proposed channel-enzyme transient receptor potential
... eLife digest Ion channels are proteins that allow specific charged particles to move across the membranes of cells – for example to travel in or out of a cell, or between different parts of the same cell. Almost all ion channels are gated, meaning that they can open and close in response to differen ...
... eLife digest Ion channels are proteins that allow specific charged particles to move across the membranes of cells – for example to travel in or out of a cell, or between different parts of the same cell. Almost all ion channels are gated, meaning that they can open and close in response to differen ...
Chemotaxis in Bacteria
... Yet both the transport and chemotaxis systems have other, independent components, and transport is not required for chemotaxis. These relationships are diagramed in Figure 2. Transport and chemotaxisare thus very closely related ; but not all substances that are transported, or for which there are b ...
... Yet both the transport and chemotaxis systems have other, independent components, and transport is not required for chemotaxis. These relationships are diagramed in Figure 2. Transport and chemotaxisare thus very closely related ; but not all substances that are transported, or for which there are b ...
Functional Analysis of Subunit e of the F1Fo
... derivatives were expressed as C-terminally HA-tagged proteins, as our present Su e-specific antiserum was raised against the C-terminal 15 amino acid residues of Su e and hence would not have recognized the truncated Su e derivatives. As a control, we also expressed the full-length Su e protein with ...
... derivatives were expressed as C-terminally HA-tagged proteins, as our present Su e-specific antiserum was raised against the C-terminal 15 amino acid residues of Su e and hence would not have recognized the truncated Su e derivatives. As a control, we also expressed the full-length Su e protein with ...
SPA Enzyme Assay Design
... The source of enzyme for activity measurements in SPA assays can be obtained from either cell lysates (crude protein) or from purified preparations (e.g., recombinant protein). In the case of crude enzyme, competing activities may also be present in the cell lysate that could affect the assay perfor ...
... The source of enzyme for activity measurements in SPA assays can be obtained from either cell lysates (crude protein) or from purified preparations (e.g., recombinant protein). In the case of crude enzyme, competing activities may also be present in the cell lysate that could affect the assay perfor ...
Stockholm University
... in a binary alphabet, i.e. exposed and buried, with an approximately equal fraction in both classes, while SABLE, ASAPglob, and ASAPmem predict the relative accessibility. Therefore, for comparisons the real value predictions were transformed to binary states. The specific cutoffs for transformation ...
... in a binary alphabet, i.e. exposed and buried, with an approximately equal fraction in both classes, while SABLE, ASAPglob, and ASAPmem predict the relative accessibility. Therefore, for comparisons the real value predictions were transformed to binary states. The specific cutoffs for transformation ...
Archaea Topics in Biodiversity
... It was first thought that most archaea were extremophiles, existing at the environmental limits of abiotic factor ranges. Recently, it has been found that there are numerous archaea living in a broad range of habitats and environmental conditions. Some Haloarchaea undergo phenotypic switching and gr ...
... It was first thought that most archaea were extremophiles, existing at the environmental limits of abiotic factor ranges. Recently, it has been found that there are numerous archaea living in a broad range of habitats and environmental conditions. Some Haloarchaea undergo phenotypic switching and gr ...
F-actin Sequesters Elongation Factor from Interaction with
... organized in a high degree of spatial order and intermediates are transferred from one enzyme to another without mixing with the surrounding cytoplasm (Stapulionis and Deutscher, 1995). A correlation between cytoplasmic alkalinization and increases in protein synthesis has been observed in a number ...
... organized in a high degree of spatial order and intermediates are transferred from one enzyme to another without mixing with the surrounding cytoplasm (Stapulionis and Deutscher, 1995). A correlation between cytoplasmic alkalinization and increases in protein synthesis has been observed in a number ...
P-type ATPase
The P-type ATPases, also known as E1-E2 ATPases, are a large group of evolutionarily related ion and lipid pumps that are found in bacteria, archaea, and eukaryotes. They are α-helical bundle primary transporters referred to as P-type ATPases because they catalyze auto- (or self-) phosphorylation of a key conserved aspartate residue within the pump. In addition, they all appear to interconvert between at least two different conformations, denoted by E1 and E2.Most members of this transporter family are specific for the pumping of a large array of cations, however one subfamily is involved in flipping phospholipids to maintain the asymmetric nature of the biomembrane.Prominent examples of P-type ATPases are the sodium-potassium pump (Na+,K+-ATPase), the plasma membrane proton pump (H+-ATPase), the proton-potassium pump (H+,K+-ATPase), and the calcium pump (Ca2+-ATPase).