Classification of Structural Protein Domain Based on Hidden Markov
... P.O. Box 80204, Jeddah 21589, Saudi Arabia. 1ORCID: ...
... P.O. Box 80204, Jeddah 21589, Saudi Arabia. 1ORCID: ...
eXtra Botany - Oxford Academic
... compound, now known to be indoleacetic acid (auxin) (see Ayres, 2008; Leyser, 2010). The functioning of auxin relates in part to its polar transport and, for tropisms, to its lateral transport. A widely accepted mechanism for polar auxin transport (PAT) is the chemiosmotic hypothesis (Rubery and Sh ...
... compound, now known to be indoleacetic acid (auxin) (see Ayres, 2008; Leyser, 2010). The functioning of auxin relates in part to its polar transport and, for tropisms, to its lateral transport. A widely accepted mechanism for polar auxin transport (PAT) is the chemiosmotic hypothesis (Rubery and Sh ...
Twins take the job
... elevated levels of hRRP6, arguing that these enzymes have partially redundant functions. ...
... elevated levels of hRRP6, arguing that these enzymes have partially redundant functions. ...
Increased and controlled expression of the Rickettsia
... identified in immunoblots of SDS-solubilized membranes of R. prowaxekii and translocase-expressing E. coli using antibodies against a synthetic peptide corresponding to the carboxyl-terminal 17 amino acids of the deduced protein (Plano & Winkler, 1991). These antibodies also bound to inside-out memb ...
... identified in immunoblots of SDS-solubilized membranes of R. prowaxekii and translocase-expressing E. coli using antibodies against a synthetic peptide corresponding to the carboxyl-terminal 17 amino acids of the deduced protein (Plano & Winkler, 1991). These antibodies also bound to inside-out memb ...
Biogenesis of hepatitis C virus envelope glycoproteins
... participate in the assembly of the infectious particle and also play a crucial role in virus entry by binding to a receptor present on the host cell and inducing fusion between the viral envelope and a membrane of the host cell. To fulfil these functions, viral envelope proteins have to adopt dramat ...
... participate in the assembly of the infectious particle and also play a crucial role in virus entry by binding to a receptor present on the host cell and inducing fusion between the viral envelope and a membrane of the host cell. To fulfil these functions, viral envelope proteins have to adopt dramat ...
Molecular Chaperones - Cellular Machines for Protein Folding
... 1.3.3. Controlled Release of Bound Polypeptides Hydrophobic interactions not only contribute to the stability of the folded structure of a protein, they are also important for the stability of oligomeric proteins and protein complexes. The contact areas often contain hydrophobic residues that become ...
... 1.3.3. Controlled Release of Bound Polypeptides Hydrophobic interactions not only contribute to the stability of the folded structure of a protein, they are also important for the stability of oligomeric proteins and protein complexes. The contact areas often contain hydrophobic residues that become ...
The ATP-Binding Cassette Transporters
... (zig-zag), and the NBD, containing the nucleotide binding fold, are shown for the three main types of full-size ABC transporters. B, Putative catalytic mechanism. The figures schematize an ABC transporter with its four domains, two of which (TMDs) are embedded in the membrane. A substrate (S) coming ...
... (zig-zag), and the NBD, containing the nucleotide binding fold, are shown for the three main types of full-size ABC transporters. B, Putative catalytic mechanism. The figures schematize an ABC transporter with its four domains, two of which (TMDs) are embedded in the membrane. A substrate (S) coming ...
The ATP-Binding Cassette Transporters: Structure, Function, and
... (zig-zag), and the NBD, containing the nucleotide binding fold, are shown for the three main types of full-size ABC transporters. B, Putative catalytic mechanism. The figures schematize an ABC transporter with its four domains, two of which (TMDs) are embedded in the membrane. A substrate (S) coming ...
... (zig-zag), and the NBD, containing the nucleotide binding fold, are shown for the three main types of full-size ABC transporters. B, Putative catalytic mechanism. The figures schematize an ABC transporter with its four domains, two of which (TMDs) are embedded in the membrane. A substrate (S) coming ...
Differential actin binding along the PEVK domain of skeletal muscle
... (PEVKIII). The nucleotide sequence boundaries of the PEVK internal volume of ~10 µl (Kellermayer, 1997; Kellermayer and segments, based on GenBank accession no. X90569 (version Granzier, 1996). First, 10 µl of HMM-PEVK mixture in assay buffer X90569.1) (Labeit and Kolmerer, 1995) are as follows: PEV ...
... (PEVKIII). The nucleotide sequence boundaries of the PEVK internal volume of ~10 µl (Kellermayer, 1997; Kellermayer and segments, based on GenBank accession no. X90569 (version Granzier, 1996). First, 10 µl of HMM-PEVK mixture in assay buffer X90569.1) (Labeit and Kolmerer, 1995) are as follows: PEV ...
Small-molecule binding sites to explore new targets in the cancer
... The transcription factor HNF4A forms a homodimer complex to interact with DNA to control the expression of other genes. In the monomer structure, two binding sites were detected on the protein surface (Fig. S5D). One of these two sites is bound to a saturated fatty acid in multiple superimposed crys ...
... The transcription factor HNF4A forms a homodimer complex to interact with DNA to control the expression of other genes. In the monomer structure, two binding sites were detected on the protein surface (Fig. S5D). One of these two sites is bound to a saturated fatty acid in multiple superimposed crys ...
Determination of Binding Sites of Cadherin Peptides on the EC1
... normally restrict the crossing of hydrophilic molecules with a molecular weight higher than 180 Da [20]. The binding between the complex of natural killer cell receptor KLRG1 and the EC1 domain of E-cadherin has been determined using X-ray crystallography and residue mutation experiments [21]. Solut ...
... normally restrict the crossing of hydrophilic molecules with a molecular weight higher than 180 Da [20]. The binding between the complex of natural killer cell receptor KLRG1 and the EC1 domain of E-cadherin has been determined using X-ray crystallography and residue mutation experiments [21]. Solut ...
The Role of Vacuole and Vacuolar H+
... investigate the roles of vacuolar H+-ATPase, plasma membrane H+-ATPase, and mitochondrial H+-ATPase in tolerance to cadmium, we examined the growth of yeast deletion mutants lacking different types of H+-ATPase in YPD media containing 80 μM of cadmium. Our results showed that a number of mutants lac ...
... investigate the roles of vacuolar H+-ATPase, plasma membrane H+-ATPase, and mitochondrial H+-ATPase in tolerance to cadmium, we examined the growth of yeast deletion mutants lacking different types of H+-ATPase in YPD media containing 80 μM of cadmium. Our results showed that a number of mutants lac ...
Chapter # PLASMA MEMBRANE PHOSPHOLIPID ASYMMETRY
... membrane biogenesis. Most of the enzymes responsible for synthesis of phospholipids and their subsequent turnover are located at sites accessible to the cytoplasmic surface of the membrane. Evidence for this has come from various approaches including the action of regulatory factors like c-Fos. cFos ...
... membrane biogenesis. Most of the enzymes responsible for synthesis of phospholipids and their subsequent turnover are located at sites accessible to the cytoplasmic surface of the membrane. Evidence for this has come from various approaches including the action of regulatory factors like c-Fos. cFos ...
Full-Text PDF
... An essential step in the determination of structure-function relationships at a molecular level is to investigate the intrinsic properties of isolated proteins, via molecular biology, biochemical and structural approaches [1–3]. However, it is also necessary to study protein function in an integrate ...
... An essential step in the determination of structure-function relationships at a molecular level is to investigate the intrinsic properties of isolated proteins, via molecular biology, biochemical and structural approaches [1–3]. However, it is also necessary to study protein function in an integrate ...
Supplementary information
... are presented in figure Sup. 1. It is clear that not only the nuclear mobility of any p53 variant is lower than this of ECFP-EYFP but also that the kinetics of fluorescence recovery is different. While p53 recovery curves are best described by the two phase exponential association model (with the ex ...
... are presented in figure Sup. 1. It is clear that not only the nuclear mobility of any p53 variant is lower than this of ECFP-EYFP but also that the kinetics of fluorescence recovery is different. While p53 recovery curves are best described by the two phase exponential association model (with the ex ...
Functions of the cytoplasmic domain of the βPS
... (4) Four highly conserved amino acid residues found in the N-terminal portion of the cytoplasmic tail are important but not critical for the developmental functions of βPS; furthermore, the efficiencies with which these mutant proteins function during different morphogenetic processes vary greatly, ...
... (4) Four highly conserved amino acid residues found in the N-terminal portion of the cytoplasmic tail are important but not critical for the developmental functions of βPS; furthermore, the efficiencies with which these mutant proteins function during different morphogenetic processes vary greatly, ...
lec31_2013 - Andrew.cmu.edu
... a. glycogen synthase (inactivating it) b. glycogen phosphorylase, activating it, leading to glycogen breakdown. B. High Blood Sugar: Proteins dephosphorylated -Glycogen synthesized, Glucose used for energy production (if needed). O H3C To reverse the above effects we need to de-phosphorylate the pro ...
... a. glycogen synthase (inactivating it) b. glycogen phosphorylase, activating it, leading to glycogen breakdown. B. High Blood Sugar: Proteins dephosphorylated -Glycogen synthesized, Glucose used for energy production (if needed). O H3C To reverse the above effects we need to de-phosphorylate the pro ...
Semester 2 - Jefferson County Public Schools
... Name the 7 taxonomic levels in Linnaeus’s hierarchy in order from largest to smallest A: What is Kingdom, Phylum, Class, Order, Family, Genus, Species ...
... Name the 7 taxonomic levels in Linnaeus’s hierarchy in order from largest to smallest A: What is Kingdom, Phylum, Class, Order, Family, Genus, Species ...
Identification and Characterization of the Acid Phosphatase HppA in
... 2 h with gentle stirring, the insoluble factions were removed by centrifugation (10,000 × g, 10 min). The supernatant was then collected as the solubilized membrane fractions. The OGP extract was concentrated and then chromatographed on a DEAE-Sepharose CLB column that was previously equilibrated wi ...
... 2 h with gentle stirring, the insoluble factions were removed by centrifugation (10,000 × g, 10 min). The supernatant was then collected as the solubilized membrane fractions. The OGP extract was concentrated and then chromatographed on a DEAE-Sepharose CLB column that was previously equilibrated wi ...
Classification Jeopardy #1
... Diagram that shows evolutionary relationships based on derived characters A: What is a cladogram? S2C06 Jeopardy Review ...
... Diagram that shows evolutionary relationships based on derived characters A: What is a cladogram? S2C06 Jeopardy Review ...
Semester 2 - Paint Valley Local Schools Home
... Diagram that shows evolutionary relationships based on derived characters A: What is a cladogram? S2C06 Jeopardy Review ...
... Diagram that shows evolutionary relationships based on derived characters A: What is a cladogram? S2C06 Jeopardy Review ...
The advantages of being small Stockholm University
... Since the 1950´s the minimal genome concept has been a challenging idea for many researchers, that is the genome believed to describe life. What is life? As stated in many textbooks, living organisms should be able to grow by themselves, react to stimuli, and self replicate, but what genes and prote ...
... Since the 1950´s the minimal genome concept has been a challenging idea for many researchers, that is the genome believed to describe life. What is life? As stated in many textbooks, living organisms should be able to grow by themselves, react to stimuli, and self replicate, but what genes and prote ...
Mapping allosteric connections from the receptor G proteins
... eterotrimeric G protein ␣ subunits function in the cell as molecular switch proteins, cycling between an inactive GDPbound heterotrimeric conformation and an active GTP-bound conformation. Heptahelical receptors in the cell membrane activate G proteins by catalyzing GTP for GDP exchange on G␣, leadi ...
... eterotrimeric G protein ␣ subunits function in the cell as molecular switch proteins, cycling between an inactive GDPbound heterotrimeric conformation and an active GTP-bound conformation. Heptahelical receptors in the cell membrane activate G proteins by catalyzing GTP for GDP exchange on G␣, leadi ...
Nitrogen Assimilation
... Glutamine Synthetase is a Primary Regulatory Point in Nitrogen Metabolism Properties of Bacterial Enzyme 12 identical 50,000 mwt subunits Combined Mwt of 600,000 ...
... Glutamine Synthetase is a Primary Regulatory Point in Nitrogen Metabolism Properties of Bacterial Enzyme 12 identical 50,000 mwt subunits Combined Mwt of 600,000 ...
Role of lipids in the translocation of proteins across membranes
... also give rise to the protonation of negatively charged side chains, facilitating their insertion into the membrane [34]. Furthermore, the size of the headgroup is of importance, i.e. bulky headgroups leave less space for proteins to insert. Regulation of acyl chain composition is important to keep ...
... also give rise to the protonation of negatively charged side chains, facilitating their insertion into the membrane [34]. Furthermore, the size of the headgroup is of importance, i.e. bulky headgroups leave less space for proteins to insert. Regulation of acyl chain composition is important to keep ...
P-type ATPase
The P-type ATPases, also known as E1-E2 ATPases, are a large group of evolutionarily related ion and lipid pumps that are found in bacteria, archaea, and eukaryotes. They are α-helical bundle primary transporters referred to as P-type ATPases because they catalyze auto- (or self-) phosphorylation of a key conserved aspartate residue within the pump. In addition, they all appear to interconvert between at least two different conformations, denoted by E1 and E2.Most members of this transporter family are specific for the pumping of a large array of cations, however one subfamily is involved in flipping phospholipids to maintain the asymmetric nature of the biomembrane.Prominent examples of P-type ATPases are the sodium-potassium pump (Na+,K+-ATPase), the plasma membrane proton pump (H+-ATPase), the proton-potassium pump (H+,K+-ATPase), and the calcium pump (Ca2+-ATPase).