8/21/08 Transcript I
... muscles of the blood vessels contract and you have to pump a lot of blood through them. o Blood pressures rises because smooth muscle contractions are long term; you don’t have high blood pressure over a couple minutes and then it goes down. o We are constantly pumping blood through ever ever smalle ...
... muscles of the blood vessels contract and you have to pump a lot of blood through them. o Blood pressures rises because smooth muscle contractions are long term; you don’t have high blood pressure over a couple minutes and then it goes down. o We are constantly pumping blood through ever ever smalle ...
The energetics, chemistry, and mechanics of a
... conditions the hydrolysis of ATP makes about 22 kB T units of energy available. So we have an efficiency of about 50–60% for kinesin’s conversion of chemical to mechanical energy. Motor protein efficiencies of about 50% is generally what has been reported by experimentalists (Howard, 2001). The powe ...
... conditions the hydrolysis of ATP makes about 22 kB T units of energy available. So we have an efficiency of about 50–60% for kinesin’s conversion of chemical to mechanical energy. Motor protein efficiencies of about 50% is generally what has been reported by experimentalists (Howard, 2001). The powe ...
Chlamydia pneumoniae CdsQ functions as a multi
... the injectisome and play a role in the selection and delivery of effector proteins through the injectisome [23, 24]. A few T3S ATPases have been characterized, including EscN from E. coli [25, 26], YscN from Yersinia [27, 28], and InvC from Salmonella [29]. The ATPase of C. pneumoniae is enzymatical ...
... the injectisome and play a role in the selection and delivery of effector proteins through the injectisome [23, 24]. A few T3S ATPases have been characterized, including EscN from E. coli [25, 26], YscN from Yersinia [27, 28], and InvC from Salmonella [29]. The ATPase of C. pneumoniae is enzymatical ...
Pore-Forming Proteins and Adaptation of Living Organisms to
... detected in venom of bees, spiders, ants, and scorpions. Structurally, they are amphiphilic α-helical peptides. Melittin (H2N-GIGAVLKVLTTGLPALISTIKRKRQQCONH2), a typical member of this group of toxins is the basic component of Apis mellifera bee venom [5]. This polypeptide is capable of killing bact ...
... detected in venom of bees, spiders, ants, and scorpions. Structurally, they are amphiphilic α-helical peptides. Melittin (H2N-GIGAVLKVLTTGLPALISTIKRKRQQCONH2), a typical member of this group of toxins is the basic component of Apis mellifera bee venom [5]. This polypeptide is capable of killing bact ...
GRASP-DNA: A Web Application to Screen Prokaryotic Genomes for
... sequence will generally be positive if there is more information in the sequence than would be expected at random. Furthermore, the higher the total score, (S), becomes then the greater the similarity between the particular sequence and the list of sites used to perform the search. An entirely alter ...
... sequence will generally be positive if there is more information in the sequence than would be expected at random. Furthermore, the higher the total score, (S), becomes then the greater the similarity between the particular sequence and the list of sites used to perform the search. An entirely alter ...
RAD50, an SMC family member with multiple roles in DNA break
... common to SMC proteins and ABC transporters. How this ATP-dependent engagement–disengagement cycle facilitates the diverse functions of the different ABC ATPases has not yet been mechanistically defined. ATP hydrolysis is essential for the SMC complexes involved in chromosome segregation and condens ...
... common to SMC proteins and ABC transporters. How this ATP-dependent engagement–disengagement cycle facilitates the diverse functions of the different ABC ATPases has not yet been mechanistically defined. ATP hydrolysis is essential for the SMC complexes involved in chromosome segregation and condens ...
Serine/threonine/tyrosine phosphorylation regulates
... STKs, Stk1 and Stk2, both implicated in regulating virulence and antibiotic resistance (Ohlsen & Donat, 2010; Tamber et al., 2010). Recently, it was established that the S. aureus Stk1 phosphorylates and regulates the activity of the global TR MgrA. MgrA belongs to the SarA/MgrA family of TRs that c ...
... STKs, Stk1 and Stk2, both implicated in regulating virulence and antibiotic resistance (Ohlsen & Donat, 2010; Tamber et al., 2010). Recently, it was established that the S. aureus Stk1 phosphorylates and regulates the activity of the global TR MgrA. MgrA belongs to the SarA/MgrA family of TRs that c ...
Molecular Chaperones in the Cytosol: from Nascent Chain to Folded
... domain of DnaJ (and of other Hsp40s) functions as a chaperone in recognizing hydrophobic peptides and can thus recruit DnaK to nascent chains (15, 43, 44). GrpE induces the release of ADP from DnaK (40), and upon rebinding of ATP the DnaK-peptide complex dissociates, completing the reaction cycle (F ...
... domain of DnaJ (and of other Hsp40s) functions as a chaperone in recognizing hydrophobic peptides and can thus recruit DnaK to nascent chains (15, 43, 44). GrpE induces the release of ADP from DnaK (40), and upon rebinding of ATP the DnaK-peptide complex dissociates, completing the reaction cycle (F ...
3.6 Enzymes - SignatureIBBiology
... metabolic pathways Some enzymes act as structural components of membranes In eukaryotic cells, some enzymes reside in specific organelles; for example, enzymes for cellular respiration are located in ...
... metabolic pathways Some enzymes act as structural components of membranes In eukaryotic cells, some enzymes reside in specific organelles; for example, enzymes for cellular respiration are located in ...
trisphosphate specifically interacts with the phox homology domain
... PLD contains a N-terminal PX domain, which can bind phosphoinositide to modulate its localization and activity. Fig. 1A shows the sequence alignment of part of the PX domain of several PX-domain-containing proteins. The PX domains of PLD have high homology with those of other proteins. To determine ...
... PLD contains a N-terminal PX domain, which can bind phosphoinositide to modulate its localization and activity. Fig. 1A shows the sequence alignment of part of the PX domain of several PX-domain-containing proteins. The PX domains of PLD have high homology with those of other proteins. To determine ...
to the complete text
... Another type of vesicular transmembrane protein is referred to as the p24 family. These are type I membrane proteins, some of which have been found in COPII- [24] and COPI-coated vesicles [25,26]. They share a common structural organization: a large lumenal domain with the propensity to form coiled- ...
... Another type of vesicular transmembrane protein is referred to as the p24 family. These are type I membrane proteins, some of which have been found in COPII- [24] and COPI-coated vesicles [25,26]. They share a common structural organization: a large lumenal domain with the propensity to form coiled- ...
Characterization of a novel phosphatidylinositol 3
... The FYVE domains have been thought of as membranetargeting domains, much like the pleckstrin homology (PH) domains that also bind to inositol phospholipids [10–12]. However, in contrast with PH domains that bind to a variety of inositol phospholipids, PtdIns3P is the only inositol phospholipid that ...
... The FYVE domains have been thought of as membranetargeting domains, much like the pleckstrin homology (PH) domains that also bind to inositol phospholipids [10–12]. However, in contrast with PH domains that bind to a variety of inositol phospholipids, PtdIns3P is the only inositol phospholipid that ...
Glycolysis
... hydroxyl group on C1 forming fructose-1,6- bisphosphate. • Enzyme: phosphofructokinase. This allosteric enzyme regulates the pace of glycolysis. • Reaction is coupled to the hydrolysis of an ATP to ADP and Pi. • This is the second irreversible reaction of the glycolytic pathway. • Reaction 4: fructo ...
... hydroxyl group on C1 forming fructose-1,6- bisphosphate. • Enzyme: phosphofructokinase. This allosteric enzyme regulates the pace of glycolysis. • Reaction is coupled to the hydrolysis of an ATP to ADP and Pi. • This is the second irreversible reaction of the glycolytic pathway. • Reaction 4: fructo ...
Solutions
... • Integral membrane proteins that “carry” 1 or more small polar substances (monosaccharides, amino acids, nucleotides…) • The movement of only one substance across a membrane is called uniport • The movement of more than one substance across a membrane is called cotransport • change their shape betw ...
... • Integral membrane proteins that “carry” 1 or more small polar substances (monosaccharides, amino acids, nucleotides…) • The movement of only one substance across a membrane is called uniport • The movement of more than one substance across a membrane is called cotransport • change their shape betw ...
Modes of Membrane Transport
... • Integral membrane proteins that “carry” 1 or more small polar substances (monosaccharides, amino acids, nucleotides…) • The movement of only one substance across a membrane is called uniport • The movement of more than one substance across a membrane is called cotransport • change their shape betw ...
... • Integral membrane proteins that “carry” 1 or more small polar substances (monosaccharides, amino acids, nucleotides…) • The movement of only one substance across a membrane is called uniport • The movement of more than one substance across a membrane is called cotransport • change their shape betw ...
Regulation of ISWI -family of chromatin remodelling complexes
... that most of fundamental regulatory aspects of nucleosome remodelling are contained within the compact ATPase module of Drosophila ISWI. In this work the authors found that ISWI lacking its HSS domain can still remodel nucleosomes, with an intrinsic ability to bind nucleosomes and functionally inter ...
... that most of fundamental regulatory aspects of nucleosome remodelling are contained within the compact ATPase module of Drosophila ISWI. In this work the authors found that ISWI lacking its HSS domain can still remodel nucleosomes, with an intrinsic ability to bind nucleosomes and functionally inter ...
ATP
... ATP is a small packet of Energy that can be used a little at a time, like coins ATP is constantly reused and recycled You can’t buy things without $$, cells can’t work/move without Energy from ATP ...
... ATP is a small packet of Energy that can be used a little at a time, like coins ATP is constantly reused and recycled You can’t buy things without $$, cells can’t work/move without Energy from ATP ...
Computational Biophysics
... in the brains of individuals with Alzheimers. A library of structures was generated using replica exchange and these structures where then clustered based on potential energy and RMSD to derive a set of representative structures. These structures were then analyzed and their predicted scalar couplin ...
... in the brains of individuals with Alzheimers. A library of structures was generated using replica exchange and these structures where then clustered based on potential energy and RMSD to derive a set of representative structures. These structures were then analyzed and their predicted scalar couplin ...
Supplementary Table VI. List of Deletions/Hypomorphic Alleles
... Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; ...
... Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; ...
The Caenorhabditis elegans mRNA 5`
... sequential enzymatic activities are required to form the "cap 0" structure, m GpppN-. First, an RNA 5'-triphosphatase (RTPase) removes the gamma-phosphate from the 5’ end of the RNA substrate to leave a diphosphate end. Next, a GTP::mRNA guanylyltransferase (GTase) catalyzes transfer of GMP from GTP ...
... sequential enzymatic activities are required to form the "cap 0" structure, m GpppN-. First, an RNA 5'-triphosphatase (RTPase) removes the gamma-phosphate from the 5’ end of the RNA substrate to leave a diphosphate end. Next, a GTP::mRNA guanylyltransferase (GTase) catalyzes transfer of GMP from GTP ...
Transport functions and physiological significance of 76 kDa Ral
... Bacterially expressed RLIP76 showed catalytic properties similar to those of DNP-SG ATPase purified from human tissues. RLIP76 had constitutive ATPase activity that was stimulated by anionic (e.g., DNP-SG), as well as cationic (e.g., DOX) ligands (Awasthi et al., 2000; 2001c; Singhal et al., 2001). ...
... Bacterially expressed RLIP76 showed catalytic properties similar to those of DNP-SG ATPase purified from human tissues. RLIP76 had constitutive ATPase activity that was stimulated by anionic (e.g., DNP-SG), as well as cationic (e.g., DOX) ligands (Awasthi et al., 2000; 2001c; Singhal et al., 2001). ...
Red Blood Cell Glycophorins
... GPA. As a consequence, these mutant RBCs maintain a net negative surface charge that is only 20% less than normal, rather than a 60% decrease, which would be expected in a GPA-deficient RBC. Individuals with En(a-) and MkMk RBCs are clinically well. Thus, increased glycosylation of band 3 in En(a-) ...
... GPA. As a consequence, these mutant RBCs maintain a net negative surface charge that is only 20% less than normal, rather than a 60% decrease, which would be expected in a GPA-deficient RBC. Individuals with En(a-) and MkMk RBCs are clinically well. Thus, increased glycosylation of band 3 in En(a-) ...
Tubular reabsorption
... Thus the molecules moves through ion channels, transporters, pumps & exchangers. Pinocytosis/ exocytosis. ...
... Thus the molecules moves through ion channels, transporters, pumps & exchangers. Pinocytosis/ exocytosis. ...
Red blood cell glycophorins
... LYCOPHORIN-A (GPA), GPB, GPC, and GPD well-characterized, search for the protein encoded by this constitute a group of red blood cell (RBC) transmemgene has yielded negative results. All the expressed glycophbrane proteins that, although perhaps not widely appreciorins are 0-glycosylated proteins wi ...
... LYCOPHORIN-A (GPA), GPB, GPC, and GPD well-characterized, search for the protein encoded by this constitute a group of red blood cell (RBC) transmemgene has yielded negative results. All the expressed glycophbrane proteins that, although perhaps not widely appreciorins are 0-glycosylated proteins wi ...
P-type ATPase
The P-type ATPases, also known as E1-E2 ATPases, are a large group of evolutionarily related ion and lipid pumps that are found in bacteria, archaea, and eukaryotes. They are α-helical bundle primary transporters referred to as P-type ATPases because they catalyze auto- (or self-) phosphorylation of a key conserved aspartate residue within the pump. In addition, they all appear to interconvert between at least two different conformations, denoted by E1 and E2.Most members of this transporter family are specific for the pumping of a large array of cations, however one subfamily is involved in flipping phospholipids to maintain the asymmetric nature of the biomembrane.Prominent examples of P-type ATPases are the sodium-potassium pump (Na+,K+-ATPase), the plasma membrane proton pump (H+-ATPase), the proton-potassium pump (H+,K+-ATPase), and the calcium pump (Ca2+-ATPase).