REVIEWS Structural insights into the function of the
... (a) Conserved domains among GDP-dissociation inhibitor (GDI) and CHM/REP family members. The schematic diagram (modified from Ref. 4) illustrates the location of sequence conserved regions (SCRs), which contain consensus motifs common between members of the GDI superfamily. The arrows indicate locat ...
... (a) Conserved domains among GDP-dissociation inhibitor (GDI) and CHM/REP family members. The schematic diagram (modified from Ref. 4) illustrates the location of sequence conserved regions (SCRs), which contain consensus motifs common between members of the GDI superfamily. The arrows indicate locat ...
sLeX inhibits Ply hemolytic activity by blocking binding of the toxin to
... SPR was used to validate glycan binding SPR analysis was also conducted with Ply truncation mutants Glycan ...
... SPR was used to validate glycan binding SPR analysis was also conducted with Ply truncation mutants Glycan ...
PapD-like chaperones and pilus biogenesis
... The crystal structure of PapD and the solution structure of FimC, and more recently, the crystal structures of the FimC᎐FimH chaperone᎐adhesin complex and the PapD᎐PapK chaperone᎐pilin complex have all been solved.25 ᎐ 28 The chaperone consists of two immunoglobulin-like ŽIg. domains oriented in an ...
... The crystal structure of PapD and the solution structure of FimC, and more recently, the crystal structures of the FimC᎐FimH chaperone᎐adhesin complex and the PapD᎐PapK chaperone᎐pilin complex have all been solved.25 ᎐ 28 The chaperone consists of two immunoglobulin-like ŽIg. domains oriented in an ...
S C T
... The membrane proteins that belong to the so-called helix bundle class, have membrane domains composed of two or more TM α-helices usually consisting of a stretch of about 15-30 amino acids with a central hydrophobic domain flanked by more polar residues (Wallin et al., 1997). The loops connecting tw ...
... The membrane proteins that belong to the so-called helix bundle class, have membrane domains composed of two or more TM α-helices usually consisting of a stretch of about 15-30 amino acids with a central hydrophobic domain flanked by more polar residues (Wallin et al., 1997). The loops connecting tw ...
Membrane immersion allows rhomboid proteases to achieve
... programmed cell death. An important characteristic of proteases is that they are highly selective, only cutting proteins that contain well-defined sequences of amino acids in accessible regions. Proteases that are soluble in water have been studied for over a century and are now well understood, as ...
... programmed cell death. An important characteristic of proteases is that they are highly selective, only cutting proteins that contain well-defined sequences of amino acids in accessible regions. Proteases that are soluble in water have been studied for over a century and are now well understood, as ...
Homologous and heterologous reconstitution of Golgi to chloroplast
... fact that precursors to the cytoplasmically synthesized light harvesting chlorophyll a/b binding protein (pLHCPII) are a group of 110, 122, 161 and 207 kDa polyproteins composed of a pre-sequence and as many as eight mature LHCPII units which are rapidly processed to mature LHCPII by a stromal pepti ...
... fact that precursors to the cytoplasmically synthesized light harvesting chlorophyll a/b binding protein (pLHCPII) are a group of 110, 122, 161 and 207 kDa polyproteins composed of a pre-sequence and as many as eight mature LHCPII units which are rapidly processed to mature LHCPII by a stromal pepti ...
Homologous and heterologous reconstitution of Golgi to chloroplast
... fact that precursors to the cytoplasmically synthesized light harvesting chlorophyll a/b binding protein (pLHCPII) are a group of 110, 122, 161 and 207 kDa polyproteins composed of a pre-sequence and as many as eight mature LHCPII units which are rapidly processed to mature LHCPII by a stromal pepti ...
... fact that precursors to the cytoplasmically synthesized light harvesting chlorophyll a/b binding protein (pLHCPII) are a group of 110, 122, 161 and 207 kDa polyproteins composed of a pre-sequence and as many as eight mature LHCPII units which are rapidly processed to mature LHCPII by a stromal pepti ...
Rab Proteins and the Organization of Organelle Membrane Domains
... Phosphoinositide metabolism and Rab GTPase function are tightly interconnected and Rab proteins can directly bind PI kinases and phosphatases to generate lipid domains enriched in specific PIs (Jean and Kiger 2012). This is exemplified by the interaction of Rab5 with two PI3 kinases and two PI phosp ...
... Phosphoinositide metabolism and Rab GTPase function are tightly interconnected and Rab proteins can directly bind PI kinases and phosphatases to generate lipid domains enriched in specific PIs (Jean and Kiger 2012). This is exemplified by the interaction of Rab5 with two PI3 kinases and two PI phosp ...
Termination of translation: interplay of mRNA, rRNAs and release
... rRNA can interact directly with stop codons in mRNA (see Arkov and Murgola, 1999). This version of the ®rst hypothesis is supported by data from several experiments. First, mutations in the small and large rRNAs strongly affect translational termination (see Green and Noller, 1997; Velichutina et al ...
... rRNA can interact directly with stop codons in mRNA (see Arkov and Murgola, 1999). This version of the ®rst hypothesis is supported by data from several experiments. First, mutations in the small and large rRNAs strongly affect translational termination (see Green and Noller, 1997; Velichutina et al ...
(DAMP) signal in plants
... DAMP SIGNAL The presence of extracellular ATP in plants was detected under various conditions (Table 2), which suggests the existence of mechanisms by which this energy molecule is released into the apoplast. Several mechanisms have been proposed for mediating ATP release (Figure 2). For example, si ...
... DAMP SIGNAL The presence of extracellular ATP in plants was detected under various conditions (Table 2), which suggests the existence of mechanisms by which this energy molecule is released into the apoplast. Several mechanisms have been proposed for mediating ATP release (Figure 2). For example, si ...
The Membrane Steps of Bacterial Cell Wall Synthesis as Antibiotic
... study, the kinetic values of BsMraY and its mutants were obtained by varying UDP-Mpp concentration between 3 and 6 mM while keeping C55-P at 1.1 mM. These concentrations are very high, and the KM values obtained were much higher than those of our own kinetic study [34]. The Kcat value of the H289R m ...
... study, the kinetic values of BsMraY and its mutants were obtained by varying UDP-Mpp concentration between 3 and 6 mM while keeping C55-P at 1.1 mM. These concentrations are very high, and the KM values obtained were much higher than those of our own kinetic study [34]. The Kcat value of the H289R m ...
Enzymatic function of nitric oxide synthases
... (Fig. 1) [6]. The former contains binding sites for one molecule each of NADPH, FAD, and FMN, in close homology with cytochrome P-450 reductase, whereas the latter binds haem and BH 4 , as well as the substrate L-arginine. Between these two regions lies the calmodulin (CaM) binding domain, which pla ...
... (Fig. 1) [6]. The former contains binding sites for one molecule each of NADPH, FAD, and FMN, in close homology with cytochrome P-450 reductase, whereas the latter binds haem and BH 4 , as well as the substrate L-arginine. Between these two regions lies the calmodulin (CaM) binding domain, which pla ...
assembly of integral membrane proteins from the periplasm into the
... OMPs are Skp, SurA, DegP, and FkpA. All of the currently discovered soluble periplasmic folding factors are bifunctional. Skp binds unfolded OMPs and LPS; SurA and FkpA have chaperone function and—independent of the chaperone function—PPIase activity. DegP is a protease and a chaperone. From the per ...
... OMPs are Skp, SurA, DegP, and FkpA. All of the currently discovered soluble periplasmic folding factors are bifunctional. Skp binds unfolded OMPs and LPS; SurA and FkpA have chaperone function and—independent of the chaperone function—PPIase activity. DegP is a protease and a chaperone. From the per ...
fulltext - DiVA Portal
... Signal conduction as well as substance exchange through membranes, involving active and passive transport of ions, macromolecules and metabolites, are predominantly mediated by proteins. They are either an integral part of the membrane bilayer or are peripherally associated with it. Proteins involve ...
... Signal conduction as well as substance exchange through membranes, involving active and passive transport of ions, macromolecules and metabolites, are predominantly mediated by proteins. They are either an integral part of the membrane bilayer or are peripherally associated with it. Proteins involve ...
How proteins produce cellular membrane curvature
... elasticity is characterized by the elastic moduli, such as the bending moduli of the membrane monolayer, κm, and bilayer, κB, the moduli of the Gaussian curvature of the monolayer and bilayer, κ m and κ B , respectively, the membrane stretching modulus, Г (see Supplementary information S1 (box) and ...
... elasticity is characterized by the elastic moduli, such as the bending moduli of the membrane monolayer, κm, and bilayer, κB, the moduli of the Gaussian curvature of the monolayer and bilayer, κ m and κ B , respectively, the membrane stretching modulus, Г (see Supplementary information S1 (box) and ...
Print - Circulation
... Na+-Ca ` exchange, it is identical to the equilibrium potential, Eeq (around -40 mV) in resting ventricular cells. The Na+-Ca`+ exchanger is known to be voltage sensitive.8 If the membrane potential is more negative than the reversal potential, as is normally the case in the resting myocyte, the exc ...
... Na+-Ca ` exchange, it is identical to the equilibrium potential, Eeq (around -40 mV) in resting ventricular cells. The Na+-Ca`+ exchanger is known to be voltage sensitive.8 If the membrane potential is more negative than the reversal potential, as is normally the case in the resting myocyte, the exc ...
Accommodating the bacterial decoding release factor as an
... protein after RNase T1 digestion that left just a dinucleotide attached to the protein, implying that the contact between the RF and the stop codon was very intimate. A detailed search for the crosslinked site on the bacterial RF2 used in the experiment involved cleavage of the factor by specific pr ...
... protein after RNase T1 digestion that left just a dinucleotide attached to the protein, implying that the contact between the RF and the stop codon was very intimate. A detailed search for the crosslinked site on the bacterial RF2 used in the experiment involved cleavage of the factor by specific pr ...
Published version
... purple, G505S; orange, G(⫺9)S; blue, G(⫺15)S. Data were fit in GraphPad Prism姞 using a non-linear fit (one site, specific binding). Error bars are not included because only one set of experiments can be carried out on a given 96-well plate, and it is not possible to average values between different ...
... purple, G505S; orange, G(⫺9)S; blue, G(⫺15)S. Data were fit in GraphPad Prism姞 using a non-linear fit (one site, specific binding). Error bars are not included because only one set of experiments can be carried out on a given 96-well plate, and it is not possible to average values between different ...
Transmembrane Fragment Structures of Amyloid Precursor Protein
... groups in the bilayer and micelle environments, along with substantial surface curvature of the surfactant/solvent interface of the micelle, introduce strain leading to a structural GG kink in the TM helix. In the bilayer there is greater integrity of the JM helix, less interhelical interaction, and ...
... groups in the bilayer and micelle environments, along with substantial surface curvature of the surfactant/solvent interface of the micelle, introduce strain leading to a structural GG kink in the TM helix. In the bilayer there is greater integrity of the JM helix, less interhelical interaction, and ...
SERCA pump level is a critical determinant of Ca2+ homeostasis
... in SERCA expression level correlate with the contractile status of the heart. The expression level of SERCA pump protein appears to be a critical determinant of cardiac contractility. In all these studies the alteration in SERCA expression is in consent with the accompanying functional changes, but ...
... in SERCA expression level correlate with the contractile status of the heart. The expression level of SERCA pump protein appears to be a critical determinant of cardiac contractility. In all these studies the alteration in SERCA expression is in consent with the accompanying functional changes, but ...
Correlating ribosome function with high
... Figure 1. The ribosome functional centers. (a) The two ribosomal subunits. Left: the small ribosomal subunit (T30S) [4]. The approximate positions of codon–anticodon interactions of A-, P- and E-tRNAs are shown and the main functional domains are indicated: H, head; L, latch; P, platform; S, shoulde ...
... Figure 1. The ribosome functional centers. (a) The two ribosomal subunits. Left: the small ribosomal subunit (T30S) [4]. The approximate positions of codon–anticodon interactions of A-, P- and E-tRNAs are shown and the main functional domains are indicated: H, head; L, latch; P, platform; S, shoulde ...
Specific Interaction of the PDZ Domain Protein PICK1 with the
... LIN-7 PDZ domain, which has been shown to mediate clustering of the receptor tyrosine kinase LET-23 (28). The PDZ domain in PICK1 appears to be relatively divergent when compared with other PDZ domains in general. As we reported previously (25), the sequence of mouse PICK1 shares significant homolog ...
... LIN-7 PDZ domain, which has been shown to mediate clustering of the receptor tyrosine kinase LET-23 (28). The PDZ domain in PICK1 appears to be relatively divergent when compared with other PDZ domains in general. As we reported previously (25), the sequence of mouse PICK1 shares significant homolog ...
THE DYNAMIN SUPERFAMILY: UNIVERSAL MEMBRANE
... thaliana ADL2 binds specifically to phosphatidylinositol-4-phosphate (PtdIns4P)30 and might, in fact, be a PH domain. Lipid binding does not exclude the possibility that this domain is also involved in protein–protein interactions. Given the absence of a PRD, recruitment to mitochondria probably occ ...
... thaliana ADL2 binds specifically to phosphatidylinositol-4-phosphate (PtdIns4P)30 and might, in fact, be a PH domain. Lipid binding does not exclude the possibility that this domain is also involved in protein–protein interactions. Given the absence of a PRD, recruitment to mitochondria probably occ ...
Cytoplasmic and cortical reorganizations of the ascidian zygote
... regions or plasms (ooplasm, protoplasm, mesoplasm) that were formed and/or localized in different regions of the zygote. He called the process cytoplasmic localization (which later came to be known as ‘ooplasmic segregation’). Conklin observed that some of the colored plasms formed during oogenesis ...
... regions or plasms (ooplasm, protoplasm, mesoplasm) that were formed and/or localized in different regions of the zygote. He called the process cytoplasmic localization (which later came to be known as ‘ooplasmic segregation’). Conklin observed that some of the colored plasms formed during oogenesis ...
Identification of A Novel, N-ethylmaleimide
... Blobel, 1989; Newmeyer and Forbes, 1990). When "S-labeled semi-intact cells and wild-type Golgi membranes were pretreated with 0.2 mM NEM on ice, the transport of man6P receptors from endosomes to the TGN was significantly inhibited (Fig. 1, compare 1 and 2). However, addition of fresh cytosol to an ...
... Blobel, 1989; Newmeyer and Forbes, 1990). When "S-labeled semi-intact cells and wild-type Golgi membranes were pretreated with 0.2 mM NEM on ice, the transport of man6P receptors from endosomes to the TGN was significantly inhibited (Fig. 1, compare 1 and 2). However, addition of fresh cytosol to an ...
P-type ATPase
The P-type ATPases, also known as E1-E2 ATPases, are a large group of evolutionarily related ion and lipid pumps that are found in bacteria, archaea, and eukaryotes. They are α-helical bundle primary transporters referred to as P-type ATPases because they catalyze auto- (or self-) phosphorylation of a key conserved aspartate residue within the pump. In addition, they all appear to interconvert between at least two different conformations, denoted by E1 and E2.Most members of this transporter family are specific for the pumping of a large array of cations, however one subfamily is involved in flipping phospholipids to maintain the asymmetric nature of the biomembrane.Prominent examples of P-type ATPases are the sodium-potassium pump (Na+,K+-ATPase), the plasma membrane proton pump (H+-ATPase), the proton-potassium pump (H+,K+-ATPase), and the calcium pump (Ca2+-ATPase).