Spotlight on the microbes that produce heat shock protein 90
... Heat shock protein 90 (Hsp90) is essential for the conformational maturation, activation and maintenance of proteins needed for all of the six hallmarks of cancer: angiogenesis, immortalization, metastasis, impaired apoptosis, insensitivity to antigrowth signals and autocrine growth [1,2]. As a resu ...
... Heat shock protein 90 (Hsp90) is essential for the conformational maturation, activation and maintenance of proteins needed for all of the six hallmarks of cancer: angiogenesis, immortalization, metastasis, impaired apoptosis, insensitivity to antigrowth signals and autocrine growth [1,2]. As a resu ...
What recent ribosome structures have revealed
... rate of peptide-bond formation by the ribosome and by a ribosomefree model system suggested that the ribosome accelerated the reaction solely by entropic effects44, which may include substrate positioning, shielding the reaction from bulk solvent, or organization of the active site44,57. A precisely ...
... rate of peptide-bond formation by the ribosome and by a ribosomefree model system suggested that the ribosome accelerated the reaction solely by entropic effects44, which may include substrate positioning, shielding the reaction from bulk solvent, or organization of the active site44,57. A precisely ...
Electron tomography of plant thylakoid membranes
... number of grana thylakoids within each stack was determined by the repeating layers of PSII dimers, which were clearly visible. In this way, it was possible to discriminate six or eight successive membrane layers in two of the grana stacks, corresponding to three or four grana disks, respectively. M ...
... number of grana thylakoids within each stack was determined by the repeating layers of PSII dimers, which were clearly visible. In this way, it was possible to discriminate six or eight successive membrane layers in two of the grana stacks, corresponding to three or four grana disks, respectively. M ...
The Role of the N-Terminal Domains of Bacterial Initiator DnaA in
... single-stranded replication eye [7,8]. The DnaA protein and oriC are also the main factors controlling the assembly of the initiation complex or are subjected to control mechanisms that restrict the number of replications to one per cell cycle [6,9,10]. It is noteworthy that in some species, e.g., E ...
... single-stranded replication eye [7,8]. The DnaA protein and oriC are also the main factors controlling the assembly of the initiation complex or are subjected to control mechanisms that restrict the number of replications to one per cell cycle [6,9,10]. It is noteworthy that in some species, e.g., E ...
Regulation of ryanodine receptors from skeletal and cardiac muscle
... mutation in pig RyRs (leading to malignant hyperthermia) causes a 3-fold reduction in the I-site affinity for Ca2+ and Mg2+.25 It is quite possible that both these regions could form the I-sites via inter-domain interactions between these widely separated sections of the amino acid sequence. The par ...
... mutation in pig RyRs (leading to malignant hyperthermia) causes a 3-fold reduction in the I-site affinity for Ca2+ and Mg2+.25 It is quite possible that both these regions could form the I-sites via inter-domain interactions between these widely separated sections of the amino acid sequence. The par ...
The contribution of the Trp/Met/Phe residues to physical interactions
... binding domain (CBD) and a p53 homotetramer. In more than 50% of all human cancers, there is a loss of p53 function by mutations that affect the DNA-binding motif, the CBD folding stability or the oligomerization state. The inter- and intra-domain interactions of p53 are critical for its association ...
... binding domain (CBD) and a p53 homotetramer. In more than 50% of all human cancers, there is a loss of p53 function by mutations that affect the DNA-binding motif, the CBD folding stability or the oligomerization state. The inter- and intra-domain interactions of p53 are critical for its association ...
BMP binding domains in the extracellular space
... homozygous for a truncated chordin allele lacking the three C-terminal CRs (CR2-4), but retaining CR1 and most of the inter-repeat region, were found to be viable hypomorphic mutants displaying only minor skeletal defects (D. B., J. Klingensmith, J. Rossant and E. M. D. R., unpublished results). Thi ...
... homozygous for a truncated chordin allele lacking the three C-terminal CRs (CR2-4), but retaining CR1 and most of the inter-repeat region, were found to be viable hypomorphic mutants displaying only minor skeletal defects (D. B., J. Klingensmith, J. Rossant and E. M. D. R., unpublished results). Thi ...
Testing of some enzymatic mixtures used for the improvement of
... toluene diisocyanate or bisdiazobenzidine derivatives can be used as cross-linking agents. The yield of immobilized enzyme is used to characterize the immobilization efficiency and is defined as the ratio of immobilized protein based on enzyme offered for binding [4]. The adsorption of an enzyme ont ...
... toluene diisocyanate or bisdiazobenzidine derivatives can be used as cross-linking agents. The yield of immobilized enzyme is used to characterize the immobilization efficiency and is defined as the ratio of immobilized protein based on enzyme offered for binding [4]. The adsorption of an enzyme ont ...
Kinesin-1–syntaphilin coupling mediates activity
... down by an anti-KHC antibody from wild-type (WT) mouse brain homogenates followed by detection with an anti-SNPH antibody. As a control, the same antibody failed to detect the SHPN–KIF5 complex from snph knockout (KO) mouse brains. (D) GST pull-down showing SNPH binding to the C-terminal tail (814–9 ...
... down by an anti-KHC antibody from wild-type (WT) mouse brain homogenates followed by detection with an anti-SNPH antibody. As a control, the same antibody failed to detect the SHPN–KIF5 complex from snph knockout (KO) mouse brains. (D) GST pull-down showing SNPH binding to the C-terminal tail (814–9 ...
Regulation of E. coli Glycogen Phosphorylase Activity by HPr
... shuttle between EI and sugar-specific proteins. EI is a 64 kDa protein consisting of an N-terminal and C-terminal domain. The N-terminal domain of EI (EIN), containing the His-189 active site, extends from residues 1 to 259 and can be phosphorylated in a fully reversible manner by phosphorylated HPr ...
... shuttle between EI and sugar-specific proteins. EI is a 64 kDa protein consisting of an N-terminal and C-terminal domain. The N-terminal domain of EI (EIN), containing the His-189 active site, extends from residues 1 to 259 and can be phosphorylated in a fully reversible manner by phosphorylated HPr ...
from dicp.ac.cn
... A/protein kinase G/protein kinase C/family kinases (AGC-type kinases), while the C-terminal kinase domain is related to the calmodulin-dependent protein kinase family (5). The molecular mechanism of MSK1 activation is compelx. It requires the phosphorylation of MSK1 at Ser360 and Thr581 by either ER ...
... A/protein kinase G/protein kinase C/family kinases (AGC-type kinases), while the C-terminal kinase domain is related to the calmodulin-dependent protein kinase family (5). The molecular mechanism of MSK1 activation is compelx. It requires the phosphorylation of MSK1 at Ser360 and Thr581 by either ER ...
CiteSeerX — Printed in Great Britain © Company of Biologists
... 15 min of incubation, the buffer was removed and the radioactivity retained by the segment of the CAM underlining the chamber was determined. Calcium uptake activities were expressed as nmole calcium/min/cm2. Extracts of CAM were assayed for the CaBP by calcium-binding activity measurements and immu ...
... 15 min of incubation, the buffer was removed and the radioactivity retained by the segment of the CAM underlining the chamber was determined. Calcium uptake activities were expressed as nmole calcium/min/cm2. Extracts of CAM were assayed for the CaBP by calcium-binding activity measurements and immu ...
Cholesterol and nicotinic acetylcholine receptor
... this neutral lipid became an omnipresent and essential component of biomembranes in Eukaryotes. The hopanoids in Prokaryotes and eukaryotic sterols share the ability to provide stability and domain compartmentalization in membranes. Even more important is the intimate association of cholesterol with ...
... this neutral lipid became an omnipresent and essential component of biomembranes in Eukaryotes. The hopanoids in Prokaryotes and eukaryotic sterols share the ability to provide stability and domain compartmentalization in membranes. Even more important is the intimate association of cholesterol with ...
Article - Andrej Sali
... et al., 2006; Selmer et al., 2006; Schuwirth et al., 2005). In addition, structures of programmed bacterial ribosomes have defined a path for the translocation of mRNA and tRNAs through the intersubunit space (Yusupova et al., 2001, 2006; Korostelev et al., 2006; Selmer et al., 2006; Berk et al., 20 ...
... et al., 2006; Selmer et al., 2006; Schuwirth et al., 2005). In addition, structures of programmed bacterial ribosomes have defined a path for the translocation of mRNA and tRNAs through the intersubunit space (Yusupova et al., 2001, 2006; Korostelev et al., 2006; Selmer et al., 2006; Berk et al., 20 ...
BAG1, a negative regulator of Hsp70 chaperone activity, uncouples
... was incubated with either Hsp70, or Hsp70 and BAG-1 in the presence or absence of ATP. In the absence of ATP, Hsp70 forms a stable complex with [125I]RCMLA, as detected in the native gel assay (Figure 2, lane 2), that rapidly dissociates upon addition of ATP (Figure 2, lanes 4–9). In striking contra ...
... was incubated with either Hsp70, or Hsp70 and BAG-1 in the presence or absence of ATP. In the absence of ATP, Hsp70 forms a stable complex with [125I]RCMLA, as detected in the native gel assay (Figure 2, lane 2), that rapidly dissociates upon addition of ATP (Figure 2, lanes 4–9). In striking contra ...
Full Article - PDF - Journal of Biotech Research
... two domains that are clearly connected by a long central loop. These domains have also been observed by Jiang et al. [30] and others [31, 32, 33, 34]. The inward and outward opening of the pore through the membrane is a major property of MFS transporter family proteins [28]. They have both the N- an ...
... two domains that are clearly connected by a long central loop. These domains have also been observed by Jiang et al. [30] and others [31, 32, 33, 34]. The inward and outward opening of the pore through the membrane is a major property of MFS transporter family proteins [28]. They have both the N- an ...
Promega Notes 39: Stabilized TMB Substrate for Horseradish
... Introduction Researchers rely upon immunological methods for the detection of specific proteins or other antigens in complex mixtures. Two popular immunodetection methods are "dot" blots, in which antigen-containing solutions are spotted directly onto a membrane, and Western blots (1,2), in which pr ...
... Introduction Researchers rely upon immunological methods for the detection of specific proteins or other antigens in complex mixtures. Two popular immunodetection methods are "dot" blots, in which antigen-containing solutions are spotted directly onto a membrane, and Western blots (1,2), in which pr ...
Role of the leader sequence in tobacco pectin methylesterase
... function both as signal and anchor for insertion into ER membrane. To visualize process of secretion, the PS-GFP construct was used, where PME PS was fused to a foreign GFP gene (Fig. 3A). After agroinjection of PS-GFP (Fig. 3B) GFP was targeted to the CW of epidermal cells. No accumulation of GFP i ...
... function both as signal and anchor for insertion into ER membrane. To visualize process of secretion, the PS-GFP construct was used, where PME PS was fused to a foreign GFP gene (Fig. 3A). After agroinjection of PS-GFP (Fig. 3B) GFP was targeted to the CW of epidermal cells. No accumulation of GFP i ...
Plasma Membrane Depolarization and Disturbed Na Homeostasis
... excitation and 510-nm emission wavelengths in a Deltascan fluorescence spectrophotometer (PTI, Monmouth Junction, NJ). A calibration curve was used to quantify [Na1]i (in millimolar). The calibration curve was made in the presence of 3 mM gramicidin in a medium containing different concentrations of ...
... excitation and 510-nm emission wavelengths in a Deltascan fluorescence spectrophotometer (PTI, Monmouth Junction, NJ). A calibration curve was used to quantify [Na1]i (in millimolar). The calibration curve was made in the presence of 3 mM gramicidin in a medium containing different concentrations of ...
isolation and characterization of the membrane
... hypodermic needle, which released the bacteroids from the membranes. This membrane then sedimented at the interface of 3 4 - 4 5 % sucrose (mean density of 1.14 g/cma). Membranes were characterized by phosphotungstic acid (PTA)chromic acid staining, ATPase activity, and localization, sensitivity to ...
... hypodermic needle, which released the bacteroids from the membranes. This membrane then sedimented at the interface of 3 4 - 4 5 % sucrose (mean density of 1.14 g/cma). Membranes were characterized by phosphotungstic acid (PTA)chromic acid staining, ATPase activity, and localization, sensitivity to ...
AraC Protein, Regulation of the L-arabinose Operon in Escherichia
... the cell, and the concentration of arabinose there depends not only on the extracellular arabinose concentration but also on the rates of arabinose uptake and catabolism. Furthermore, these two rates depend on the level of expression of the proteins that are controlled by AraC. Consequently, the sys ...
... the cell, and the concentration of arabinose there depends not only on the extracellular arabinose concentration but also on the rates of arabinose uptake and catabolism. Furthermore, these two rates depend on the level of expression of the proteins that are controlled by AraC. Consequently, the sys ...
HER2
... • Connects the αC helix with the A-loop, stabilizing the helix in the active site • Bond is conserved among ErbB family members: K851-E734 (EGFR), K856-E739 (ErbB4) ...
... • Connects the αC helix with the A-loop, stabilizing the helix in the active site • Bond is conserved among ErbB family members: K851-E734 (EGFR), K856-E739 (ErbB4) ...
Immobilized Enzyme Technology: Potentiality and Prospects
... It is an irreversible method of enzyme immobilization (Figure 1g). It is different from other techniques in the sense that it does not require a support for the immobilization. There are two methods of cross linking in use, (i) Cross Linking Enzyme Aggregate (CLEA), and (ii) Cross Linking Enzyme Cry ...
... It is an irreversible method of enzyme immobilization (Figure 1g). It is different from other techniques in the sense that it does not require a support for the immobilization. There are two methods of cross linking in use, (i) Cross Linking Enzyme Aggregate (CLEA), and (ii) Cross Linking Enzyme Cry ...
Bile Formation: a Concerted Action of Membrane
... cations (type I) and bulkier, divalent, more lipophilic cations (type II). Respective examples are tri-n-butylmethylammonium or vecuronium and quinine. Other transporters with higher substrate selectivity have also been identified, e.g., for thiamine or N-methylnicotinamide. Some substrates exhibit ...
... cations (type I) and bulkier, divalent, more lipophilic cations (type II). Respective examples are tri-n-butylmethylammonium or vecuronium and quinine. Other transporters with higher substrate selectivity have also been identified, e.g., for thiamine or N-methylnicotinamide. Some substrates exhibit ...
PDF - Oxford Academic - Oxford University Press
... probable to arise by chance during evolution. Furthermore, SDPpred does not require information about the 3D protein structure, which instead can be used at the post-processing step to validate the predictions. There are also other approaches for comparative analysis of protein subfamilies. One of t ...
... probable to arise by chance during evolution. Furthermore, SDPpred does not require information about the 3D protein structure, which instead can be used at the post-processing step to validate the predictions. There are also other approaches for comparative analysis of protein subfamilies. One of t ...
P-type ATPase
The P-type ATPases, also known as E1-E2 ATPases, are a large group of evolutionarily related ion and lipid pumps that are found in bacteria, archaea, and eukaryotes. They are α-helical bundle primary transporters referred to as P-type ATPases because they catalyze auto- (or self-) phosphorylation of a key conserved aspartate residue within the pump. In addition, they all appear to interconvert between at least two different conformations, denoted by E1 and E2.Most members of this transporter family are specific for the pumping of a large array of cations, however one subfamily is involved in flipping phospholipids to maintain the asymmetric nature of the biomembrane.Prominent examples of P-type ATPases are the sodium-potassium pump (Na+,K+-ATPase), the plasma membrane proton pump (H+-ATPase), the proton-potassium pump (H+,K+-ATPase), and the calcium pump (Ca2+-ATPase).