Full-Text PDF
... receptors are, currently, the focus of detailed biophysical and biochemical investigations directed to elucidate the relation between ligand binding and functional properties, or to resolve structure activity relationships [7]. Biological membranes are the first fence that has to be overcome by toxi ...
... receptors are, currently, the focus of detailed biophysical and biochemical investigations directed to elucidate the relation between ligand binding and functional properties, or to resolve structure activity relationships [7]. Biological membranes are the first fence that has to be overcome by toxi ...
Phylogenetic Classification of Protozoa Based on the
... packs tightly against the opposite monomer on the back side of its DHFR active site using mainly hydrophobic interactions (Table II). Clearly, all of the interactions of the donated helix are important in domain stabilization. The donated helix may be implicated in the mechanism of the bifunctional ...
... packs tightly against the opposite monomer on the back side of its DHFR active site using mainly hydrophobic interactions (Table II). Clearly, all of the interactions of the donated helix are important in domain stabilization. The donated helix may be implicated in the mechanism of the bifunctional ...
Phospholipid Class and Fatty Acid Composition of Golgi Apparatus
... precursor particles within tubules and forming secretory vesicles of isolated Golgi apparatus partially explains the high neutral lipid content of this fraction (MorrC et al., 1969a,b). Hence, phospholipids of washed Golgi apparatus preparations are of particular interest since they can reasonably b ...
... precursor particles within tubules and forming secretory vesicles of isolated Golgi apparatus partially explains the high neutral lipid content of this fraction (MorrC et al., 1969a,b). Hence, phospholipids of washed Golgi apparatus preparations are of particular interest since they can reasonably b ...
Subcellular Trafficking of Mammalian Lysosomal Proteins: An
... M(X)9G and WE motifs mentioned above). This point is further supported when looking at MLN64, a lysosomal protein involved in cholesterol transport, whose sorting depends on a KSASNP motif located in its C-terminal START domain [34]. This motif mediates binding to the cytosolic protein 14-3-3 indepe ...
... M(X)9G and WE motifs mentioned above). This point is further supported when looking at MLN64, a lysosomal protein involved in cholesterol transport, whose sorting depends on a KSASNP motif located in its C-terminal START domain [34]. This motif mediates binding to the cytosolic protein 14-3-3 indepe ...
RNA helicase DDX19 stabilizes ribosomal elongation and
... in the cell has immediate negative effects on protein biosynthesis. It is important to note that the cellular concentration of eRF3 does not influence the rate of translation (a 60% decrease had no significant effect). These data confirm the important role of Dbp5 during translation in yeast. More r ...
... in the cell has immediate negative effects on protein biosynthesis. It is important to note that the cellular concentration of eRF3 does not influence the rate of translation (a 60% decrease had no significant effect). These data confirm the important role of Dbp5 during translation in yeast. More r ...
Human Cardiac Troponin Complex. Structure and Functions
... as to impede the actomyosin complex formation [76, 77]. A few variants of the inhibitory domain structure are presented in the literature. In the work of Brown et al. [74] on artificial troponin complex composed of different animal troponins, by means of site-directed spin labeling (SDSL-EPR) it was ...
... as to impede the actomyosin complex formation [76, 77]. A few variants of the inhibitory domain structure are presented in the literature. In the work of Brown et al. [74] on artificial troponin complex composed of different animal troponins, by means of site-directed spin labeling (SDSL-EPR) it was ...
Phylogenetic Classification of Protozoa Based on the Structure of
... DHFR-TS proteins, notably from Apicomplexan protozoa, include a long linker between the DHFR and TS domains. The linker polypeptide, even within the Apicomplexans, varies significantly in length; the entire linker in C. hominis is 58 amino acids, the linker in Toxoplasma gondii is 72 amino acids, an ...
... DHFR-TS proteins, notably from Apicomplexan protozoa, include a long linker between the DHFR and TS domains. The linker polypeptide, even within the Apicomplexans, varies significantly in length; the entire linker in C. hominis is 58 amino acids, the linker in Toxoplasma gondii is 72 amino acids, an ...
A Molecular Switch for Targeting between Endoplasmic Reticulum
... and encodes a protein that is targeted to the mitochondria. The second splice variant is found in isoforms 1b and 1d, initiates transcription at exon 2, and encodes a protein that is targeted to the ER. In addition to alternative splicing, initiation of translation from different codons provides an ...
... and encodes a protein that is targeted to the mitochondria. The second splice variant is found in isoforms 1b and 1d, initiates transcription at exon 2, and encodes a protein that is targeted to the ER. In addition to alternative splicing, initiation of translation from different codons provides an ...
Glycoprotein IIIa Is Phosphorylated in Intact Human
... 1). The purified material was essentially homogeneous (Fig 1, lane 1). Only one component of the complex was phosphorylated. This was identified as G P IIIa (lanes 2 and 4) on the basis of its co-electrophoresis with iodinated G P IIIa (lanes 3 and 5) from the purified G P IIb-IIIa complex. The poss ...
... 1). The purified material was essentially homogeneous (Fig 1, lane 1). Only one component of the complex was phosphorylated. This was identified as G P IIIa (lanes 2 and 4) on the basis of its co-electrophoresis with iodinated G P IIIa (lanes 3 and 5) from the purified G P IIb-IIIa complex. The poss ...
The ribosome — a macromolecular machine par excellence
... As in the case of EF-G, the action of EF-Tu requires energy released by GTP hydrolysis. Much of this information has been known for years (see [5]), but the way the various components of the protein synthetic machinery interact in three dimensions remained a mystery until the advent of cryo-electron ...
... As in the case of EF-G, the action of EF-Tu requires energy released by GTP hydrolysis. Much of this information has been known for years (see [5]), but the way the various components of the protein synthetic machinery interact in three dimensions remained a mystery until the advent of cryo-electron ...
The Physiological Significance of Mitochondrial Proton Leak in
... isolated from a poikilotherm is much lower than that of a homeotherm of equivalent body size and preferred body temperature might indicate that proton leak is involved in thermoregulation. However, the fact that the balance between leak and other oxygen consuming processes is the same in liver cells ...
... isolated from a poikilotherm is much lower than that of a homeotherm of equivalent body size and preferred body temperature might indicate that proton leak is involved in thermoregulation. However, the fact that the balance between leak and other oxygen consuming processes is the same in liver cells ...
Ribosome engineering to promote new crystal forms
... create a construct with a C-terminal His tag containing a TEV site and overexpressed in E. coli strain BL21 (DE3). The cells were lysed in lysis buffer (50 mM sodium phosphate pH 8.0, 0.3 M NaCl, 5 mM -mercaptoethanol) using an Emulsiflex (Avestin, Ottawa, Canada) and the cell debris was pelleted b ...
... create a construct with a C-terminal His tag containing a TEV site and overexpressed in E. coli strain BL21 (DE3). The cells were lysed in lysis buffer (50 mM sodium phosphate pH 8.0, 0.3 M NaCl, 5 mM -mercaptoethanol) using an Emulsiflex (Avestin, Ottawa, Canada) and the cell debris was pelleted b ...
Sains Dasar Biologi – Kimia – Biodiversitas
... ball whose wall is composed of hundreds or thousands of biflagellated cells (see inset LM) embedded in a gelatinous matrix. The cells are usually connected by strands of cytoplasm; if isolated, these cells cannot reproduce. The large colonies seen here will eventually release the small “daughter” co ...
... ball whose wall is composed of hundreds or thousands of biflagellated cells (see inset LM) embedded in a gelatinous matrix. The cells are usually connected by strands of cytoplasm; if isolated, these cells cannot reproduce. The large colonies seen here will eventually release the small “daughter” co ...
Transcription • Transcription factors • Chromatin • RNA polymerase II
... block recruitment of pol II, emphasizing that the CTD code is read differently before and after initiation of transcription. (b) The CTD is located close to the RNA exit channel [16]. Phosphorylation of serine residue in position 5 (Ser5), by the CDK7 subunit of the general transcription factor TFII ...
... block recruitment of pol II, emphasizing that the CTD code is read differently before and after initiation of transcription. (b) The CTD is located close to the RNA exit channel [16]. Phosphorylation of serine residue in position 5 (Ser5), by the CDK7 subunit of the general transcription factor TFII ...
biochemical model for enhanced biological phosphorus removal
... ATP by the membrane-bound ATP-ase enzyme complex, and for the transport of substrates as discussed in the next section. Three major mechanisms are used by most bacteria to translocate protons and maintain a pmf (see Fig. 4). The first one is of major importance and makes use of the cytoplasmic membr ...
... ATP by the membrane-bound ATP-ase enzyme complex, and for the transport of substrates as discussed in the next section. Three major mechanisms are used by most bacteria to translocate protons and maintain a pmf (see Fig. 4). The first one is of major importance and makes use of the cytoplasmic membr ...
2.2.3 Enzymes
... speed up these chemical reactions by acting as catalysts. A catalyst speeds up or slows down a chemical reaction without being used up itself. Enzymes like proteins are made of long chains of amino acids – polypeptide chains. There are 4 levels of protein structure: Primary Secondary Tertiary ...
... speed up these chemical reactions by acting as catalysts. A catalyst speeds up or slows down a chemical reaction without being used up itself. Enzymes like proteins are made of long chains of amino acids – polypeptide chains. There are 4 levels of protein structure: Primary Secondary Tertiary ...
Structure based hypothesis of a mitochondrial
... stop codon U1 recognition, to isoleucine. While Glu-119 points outwards, the most likely side chain conformation for isoleucine at this position points directly into the nucleotide binding region. As such it would prevent nucleotides from binding and making backbone hydrogen bonding interactions lik ...
... stop codon U1 recognition, to isoleucine. While Glu-119 points outwards, the most likely side chain conformation for isoleucine at this position points directly into the nucleotide binding region. As such it would prevent nucleotides from binding and making backbone hydrogen bonding interactions lik ...
Structural Basis for Bivalent Smac-Mimetics Recognition in the IAP
... composed of eight BIR3 molecules (XIAP residues 253 ± 2 through 351 ± 5) and eight compound 3 molecules, each of which has an inhibitory head bound to BIR3 and the other devoid of any contact to the protein. The eight asymmetric unit copies of the XIAP-BIR3/compound 3 complex can be divided into two ...
... composed of eight BIR3 molecules (XIAP residues 253 ± 2 through 351 ± 5) and eight compound 3 molecules, each of which has an inhibitory head bound to BIR3 and the other devoid of any contact to the protein. The eight asymmetric unit copies of the XIAP-BIR3/compound 3 complex can be divided into two ...
Brassinosteroid Signal Transduction from Receptor Kinases to
... plants. Genetic studies in Arabidopsis illustrated the essential roles of BRs in a wide range of developmental processes and helped identify many genes involved in BR biosynthesis and signal transduction. Recently, proteomic studies identified missing links. Together, these approaches established the ...
... plants. Genetic studies in Arabidopsis illustrated the essential roles of BRs in a wide range of developmental processes and helped identify many genes involved in BR biosynthesis and signal transduction. Recently, proteomic studies identified missing links. Together, these approaches established the ...
Regulation of the Function of the Human ABCG2 Multidrug
... especially high in the intestinal epithelial cells and in hepatocytes. The interaction between ABCG2 and bile acids has been extensively investigated, but these studies have yielded controversial data. Two groups have reported that there is no interaction with ABCG2 or direct transport of taurochola ...
... especially high in the intestinal epithelial cells and in hepatocytes. The interaction between ABCG2 and bile acids has been extensively investigated, but these studies have yielded controversial data. Two groups have reported that there is no interaction with ABCG2 or direct transport of taurochola ...
MODified™ Protein Domain Binding Kit Manual
... epigenetic marks play a role in the regulation of gene expression and chromatin state1-4. These histone modifications are recognized and bound by specific proteins that are coined ‘writers’ ‘readers’ and ‘erasers’. Many of these proteins contain highly conserved domains which serve as the building b ...
... epigenetic marks play a role in the regulation of gene expression and chromatin state1-4. These histone modifications are recognized and bound by specific proteins that are coined ‘writers’ ‘readers’ and ‘erasers’. Many of these proteins contain highly conserved domains which serve as the building b ...
Allosteric Modulators of Steroid Hormone Receptors: Structural
... molecule (reviewed in Ref. 60). From analysis of the amino acid sequences of SHR-like proteins from different organisms, including invertebrates such as molluscs and annelids, it has been proposed that the ancestral gene for SHRs was ER-like and bound estrogens (reviewed in Ref. 61). The binding of ...
... molecule (reviewed in Ref. 60). From analysis of the amino acid sequences of SHR-like proteins from different organisms, including invertebrates such as molluscs and annelids, it has been proposed that the ancestral gene for SHRs was ER-like and bound estrogens (reviewed in Ref. 61). The binding of ...
Ammodytoxins, Potent Presynaptic Neurotoxins
... Presynaptically acting sPLA2 neurotoxins interfere specifically with the release of acetylcholine from motoneurons, and their PLA2 activity is essential for the irreversible blockade of neuromuscular transmission (5, 6). It has been suggested that structurally different sPLA2 neurotoxins from differ ...
... Presynaptically acting sPLA2 neurotoxins interfere specifically with the release of acetylcholine from motoneurons, and their PLA2 activity is essential for the irreversible blockade of neuromuscular transmission (5, 6). It has been suggested that structurally different sPLA2 neurotoxins from differ ...
Single Processing Center Models for Human Dicer and Bacterial
... tially cleaves off siRNAs from the termini of dsRNA substrates (Zhang et al., 2002). We took advantage of this observation to map cleavage sites introduced by the wt and mutant proteins in the 30 bp substrate. The 30 bp dsRNA can be cleaved by Dicer only once yielding the ⵑ21 nt siRNA-like products ...
... tially cleaves off siRNAs from the termini of dsRNA substrates (Zhang et al., 2002). We took advantage of this observation to map cleavage sites introduced by the wt and mutant proteins in the 30 bp substrate. The 30 bp dsRNA can be cleaved by Dicer only once yielding the ⵑ21 nt siRNA-like products ...
Poster
... According to the CDC, 34.9% of United States adults are obese, which is linked to premature death, heart disease, cancer, respiratory disorders, fertility problems, Type 2 diabetes, and stroke. Over- and under-eating are related to brain chemistry. A 38 amino acid peptide hormone in the hypothalamus ...
... According to the CDC, 34.9% of United States adults are obese, which is linked to premature death, heart disease, cancer, respiratory disorders, fertility problems, Type 2 diabetes, and stroke. Over- and under-eating are related to brain chemistry. A 38 amino acid peptide hormone in the hypothalamus ...
P-type ATPase
The P-type ATPases, also known as E1-E2 ATPases, are a large group of evolutionarily related ion and lipid pumps that are found in bacteria, archaea, and eukaryotes. They are α-helical bundle primary transporters referred to as P-type ATPases because they catalyze auto- (or self-) phosphorylation of a key conserved aspartate residue within the pump. In addition, they all appear to interconvert between at least two different conformations, denoted by E1 and E2.Most members of this transporter family are specific for the pumping of a large array of cations, however one subfamily is involved in flipping phospholipids to maintain the asymmetric nature of the biomembrane.Prominent examples of P-type ATPases are the sodium-potassium pump (Na+,K+-ATPase), the plasma membrane proton pump (H+-ATPase), the proton-potassium pump (H+,K+-ATPase), and the calcium pump (Ca2+-ATPase).