Role of Polo-like kinase in the degradation of early mitotic inhibitor 1
... and New York University Cancer Institute, New York University School of Medicine, New York, NY 10016 Contributed by Avram Hershko, April 9, 2004 ...
... and New York University Cancer Institute, New York University School of Medicine, New York, NY 10016 Contributed by Avram Hershko, April 9, 2004 ...
Extracellular matrix of the charophycean green algae
... flaccidum, and Spirogyra spp. exhibits significant biochemical diversity, ranging from distinct “land plant” polymers to polysaccharides unique to these algae. The neutral sugar composition of Chlorokybus atmophyticus hot water extract and Spirogyra extracellular polymeric substance (EPS), combined ...
... flaccidum, and Spirogyra spp. exhibits significant biochemical diversity, ranging from distinct “land plant” polymers to polysaccharides unique to these algae. The neutral sugar composition of Chlorokybus atmophyticus hot water extract and Spirogyra extracellular polymeric substance (EPS), combined ...
Identification of Hedgehog Pathway Components by RNAi in
... genes. Two gene products reported to function in Wingless ( Wg) signaling were identified as Hh pathway components: a cell surface protein (Dally-like protein) required for Hh signal reception, and casein kinase 1␣, a candidate tumor suppressor that regulates basal activities of both Hh and Wg pathwa ...
... genes. Two gene products reported to function in Wingless ( Wg) signaling were identified as Hh pathway components: a cell surface protein (Dally-like protein) required for Hh signal reception, and casein kinase 1␣, a candidate tumor suppressor that regulates basal activities of both Hh and Wg pathwa ...
Plant development, auxin, and the subsystem
... for example AUX1, which appears to be distributed uniformly in the cell membranes of Arabidopsis root cells (Bennett et al., 1996). Once within a cell, IAA is transported in a polar or in a non-polar manner (Figure 1). Auxin polar transport involves an IAA-influx carrier protein encoded by the AUX1 g ...
... for example AUX1, which appears to be distributed uniformly in the cell membranes of Arabidopsis root cells (Bennett et al., 1996). Once within a cell, IAA is transported in a polar or in a non-polar manner (Figure 1). Auxin polar transport involves an IAA-influx carrier protein encoded by the AUX1 g ...
PDF - Oxford Academic - Oxford University Press
... However, PrpS appears to act as a unique class of ‘receptor’ that interacts with PrsS in a very specific manner, triggering an intracellular signalling network and resulting in a highly specific biological response, as will be described. Analysis of the SI-triggered events has been enabled by a uniq ...
... However, PrpS appears to act as a unique class of ‘receptor’ that interacts with PrsS in a very specific manner, triggering an intracellular signalling network and resulting in a highly specific biological response, as will be described. Analysis of the SI-triggered events has been enabled by a uniq ...
Drosophila embryos close epithelial wounds using a combination of
... myosin II recruitment relative to actin by examining embryos coexpressing a myosin regulatory light chain (sqh) fusion to GFP (sqh-GFP) and actin (sChMCA). Myosin II can be detected at the wound edge 5 minutes after wounding, overlapping with actin accumulation along the leading edge (Fig. 3A–B9; su ...
... myosin II recruitment relative to actin by examining embryos coexpressing a myosin regulatory light chain (sqh) fusion to GFP (sqh-GFP) and actin (sChMCA). Myosin II can be detected at the wound edge 5 minutes after wounding, overlapping with actin accumulation along the leading edge (Fig. 3A–B9; su ...
(ECM) Binding Sites on the Basal Surface of Embryonic Corneal
... © The Rockefeller University Press, 0021-9525/86/05/1907/10 $1.00 The Journal of Cell Biology, Volume 102, May 1986 1907-1916 ...
... © The Rockefeller University Press, 0021-9525/86/05/1907/10 $1.00 The Journal of Cell Biology, Volume 102, May 1986 1907-1916 ...
Notch and the Awesome Power of Genetics
... The simple cellular anatomy and invariant cell lineage of wild-type hermaphrodites allows mutants to be understood in terms of altered cell-fate decisions by individual cells (Horvitz and Sulston 1980; Sulston and Horvitz 1981). When Paul Sternberg, with his encyclopedic knowledge of postembryonic w ...
... The simple cellular anatomy and invariant cell lineage of wild-type hermaphrodites allows mutants to be understood in terms of altered cell-fate decisions by individual cells (Horvitz and Sulston 1980; Sulston and Horvitz 1981). When Paul Sternberg, with his encyclopedic knowledge of postembryonic w ...
Molecular Definition of Distinct Cytoskeletal Structures
... Materials. PMA, chelerythrine, herbimycin and lavendustin were purchased from LC Laboratories (Woburn, MA). FBS and bovine calf serum (CBS) were from HyClone Laboratories (Logan, UT). Bio-Gel P-100 was from Bio-Rad (Melville, NY). SRBCs and rabbit anti-SRBC lgG and IgM were from Cappel/ ...
... Materials. PMA, chelerythrine, herbimycin and lavendustin were purchased from LC Laboratories (Woburn, MA). FBS and bovine calf serum (CBS) were from HyClone Laboratories (Logan, UT). Bio-Gel P-100 was from Bio-Rad (Melville, NY). SRBCs and rabbit anti-SRBC lgG and IgM were from Cappel/ ...
Spindle pole body-anchored Kar3 drives the nucleus
... these elements interact to produce the forces necessary for nuclear migration is less clear. We used electron tomography, molecular genetics, quantitative imaging, and first principles modeling to investigate how cytoplasmic microtubules are organized during nuclear congression. We found that Kar3, ...
... these elements interact to produce the forces necessary for nuclear migration is less clear. We used electron tomography, molecular genetics, quantitative imaging, and first principles modeling to investigate how cytoplasmic microtubules are organized during nuclear congression. We found that Kar3, ...
Effect of nanoparticles on the activity of the electrone ion pumps in
... respiration and so on ). Therefore, the studying of MP kinetics in cells with this or other factor influence gives information about active ion pumps function in plasmatic membrane. As noted above two types of active proton pumps parallel activate in plant cells. One of these is H-ATFase proton comp ...
... respiration and so on ). Therefore, the studying of MP kinetics in cells with this or other factor influence gives information about active ion pumps function in plasmatic membrane. As noted above two types of active proton pumps parallel activate in plant cells. One of these is H-ATFase proton comp ...
Mosses as model systems for the study of metabolism and
... on the induction of caulonemal filaments. In Saccharomyces cerevisiae, the product of the Hxk2 gene, the major hexokinase, also plays a role in glucose repression. A protein kinase encoded by Snf1 forms part of the chain leading to the transcriptional shut down of carbon catabolism in the presence o ...
... on the induction of caulonemal filaments. In Saccharomyces cerevisiae, the product of the Hxk2 gene, the major hexokinase, also plays a role in glucose repression. A protein kinase encoded by Snf1 forms part of the chain leading to the transcriptional shut down of carbon catabolism in the presence o ...
Pex5p stabilizes Pex14p: a study using a newly isolated pex5 CHO
... understanding of multiple functions of mammalian Pex5p. In mammals, two types of Pex5p isoforms have been identified: a shorter one (Pex5pS) and a longer one (Pex5pL) with a 37amino acid insertion at the N-terminal region [9,10]. By using a Pex5 CHO mutant, ZP105, defective in both PTS1 and PTS2 imp ...
... understanding of multiple functions of mammalian Pex5p. In mammals, two types of Pex5p isoforms have been identified: a shorter one (Pex5pS) and a longer one (Pex5pL) with a 37amino acid insertion at the N-terminal region [9,10]. By using a Pex5 CHO mutant, ZP105, defective in both PTS1 and PTS2 imp ...
Plant Cell
... The ROS wave is shown to be mediated by the RBOHD proteins of each individual cell along its path. This process is integrated with the Ca2+ wave through the function of TPC1 or different ROS-regulated Ca2+ channels in a process termed ROS-induced Ca2+ release (RICR). The elevated Ca2+ levels are sho ...
... The ROS wave is shown to be mediated by the RBOHD proteins of each individual cell along its path. This process is integrated with the Ca2+ wave through the function of TPC1 or different ROS-regulated Ca2+ channels in a process termed ROS-induced Ca2+ release (RICR). The elevated Ca2+ levels are sho ...
Skeletal Muscle Stem Cells in the Spotlight: The Satellite Cell
... basis of being negative for CD45, CD31, and Sca1, and positive for CD34 and a7 integrin [57, 58]. Additional cell surface antigens, including CXCR4, b1 integrin, and syndecan-4 have also been utilized for isolation of myogenic progenitors from adult muscle [56, 68]. A range of fluorescence-based rep ...
... basis of being negative for CD45, CD31, and Sca1, and positive for CD34 and a7 integrin [57, 58]. Additional cell surface antigens, including CXCR4, b1 integrin, and syndecan-4 have also been utilized for isolation of myogenic progenitors from adult muscle [56, 68]. A range of fluorescence-based rep ...
Synergistic interaction of three ERECTA-family
... clones for ERL1 and ERL2 by a combination of RT-PCR and 5′ RACE-PCR (Fig. 1A). Among the 223 Arabidopsis genes encoding LRR-RLKs (Shiu and Bleecker, 2003), ERECTA possesses an unusual, characteristic exon-intron structure with 26 introns (Torii et al., 1996). A comparison of genomic and cDNA sequenc ...
... clones for ERL1 and ERL2 by a combination of RT-PCR and 5′ RACE-PCR (Fig. 1A). Among the 223 Arabidopsis genes encoding LRR-RLKs (Shiu and Bleecker, 2003), ERECTA possesses an unusual, characteristic exon-intron structure with 26 introns (Torii et al., 1996). A comparison of genomic and cDNA sequenc ...
Emerging Themes of Plant Signal Transduction
... controlled by a network of positively and negatively acting elements. Whereas the positively acting intermediates are essential to drive signal transduction, the negative elements are responsible for ensuring a response that is quantitatively appropriate, correctly timed, and highly coordinated with ...
... controlled by a network of positively and negatively acting elements. Whereas the positively acting intermediates are essential to drive signal transduction, the negative elements are responsible for ensuring a response that is quantitatively appropriate, correctly timed, and highly coordinated with ...
Signaling Mechanisms That Regulate Smooth Muscle Cell
... Interestingly, S1PR2-deficient mice do not show an overt SMC phenotype, but Shimizu et al have recently demonstrated that they are more susceptible to injury-induced restenosis,87 suggesting that S1P signaling through this pathway helps maintain SMC differentiation in vivo. Angiotensin II– dependent ...
... Interestingly, S1PR2-deficient mice do not show an overt SMC phenotype, but Shimizu et al have recently demonstrated that they are more susceptible to injury-induced restenosis,87 suggesting that S1P signaling through this pathway helps maintain SMC differentiation in vivo. Angiotensin II– dependent ...
during T Lymphocyte Activation Coordinately Regulated by ERK
... The assay for glutaminase activity was adapted from Graham and Aprison (22) and Ardawi and Newsholme (23). Resting or stimulated T cells were lysed by sonication in 150 mM potassium phosphate buffer (an equimolar mixture of K2HPO4 and KH2PO4), 50 mM Tris-HCl (pH 8.6), 1 mM EDTA, and 1% Triton X-100 ...
... The assay for glutaminase activity was adapted from Graham and Aprison (22) and Ardawi and Newsholme (23). Resting or stimulated T cells were lysed by sonication in 150 mM potassium phosphate buffer (an equimolar mixture of K2HPO4 and KH2PO4), 50 mM Tris-HCl (pH 8.6), 1 mM EDTA, and 1% Triton X-100 ...
Nucleolar localization of an isoform of the IGF
... this as an effect of the strength of the secretory signal peptide in competition with the nuclear and nucleolar localization signal(s). To exclude the possibility that the exon 2encoded signal peptide was directly involved in the nuclear and nucleolar localization, it was deleted from the constructi ...
... this as an effect of the strength of the secretory signal peptide in competition with the nuclear and nucleolar localization signal(s). To exclude the possibility that the exon 2encoded signal peptide was directly involved in the nuclear and nucleolar localization, it was deleted from the constructi ...
The Arf and Rab11 effector FIP3 acts synergistically with ASAP1 to
... contains only a portion of the Arf5/6 binding site (Inoue et al., 2008). This indicates that the Arf4 binding site on FIP3 is within amino acids 666–756, closer to the Rab11 binding site. We sought to determine whether ASAP1, in addition to recruiting Rab11a (Wang et al., 2012), specifically recruit ...
... contains only a portion of the Arf5/6 binding site (Inoue et al., 2008). This indicates that the Arf4 binding site on FIP3 is within amino acids 666–756, closer to the Rab11 binding site. We sought to determine whether ASAP1, in addition to recruiting Rab11a (Wang et al., 2012), specifically recruit ...
Antibodies in plantscience
... Primary screening of antibodies. Measurement of antigens; usual to ...
... Primary screening of antibodies. Measurement of antigens; usual to ...
Sarcolemmal cholesterol and caveolin-3 dependence of - AJP
... Data are means ⫾ SE. Values were recorded before pacing and methyl--cyclodextrin (MCD) infusion. LVEDP, left ventricular (LV) end-diastolic pressure; LVDP, LV developed pressure; AM, acute morphine treatment; SLP, sustained ligand-activated preconditioning. AJP-Heart Circ Physiol • doi:10.1152/ajp ...
... Data are means ⫾ SE. Values were recorded before pacing and methyl--cyclodextrin (MCD) infusion. LVEDP, left ventricular (LV) end-diastolic pressure; LVDP, LV developed pressure; AM, acute morphine treatment; SLP, sustained ligand-activated preconditioning. AJP-Heart Circ Physiol • doi:10.1152/ajp ...
Chapter 31: Fungi
... • fungus gets free carbs! • algae or cyanobacteria protected from the elements ...
... • fungus gets free carbs! • algae or cyanobacteria protected from the elements ...
Storage globulins pass through the Golgi apparatus and
... storage vacuoles (PSVs). Multiple mechanisms have been proposed to be responsible for transporting storage proteins to PSVs in developing seeds. In this study, a specific antibody was raised against the mung bean (Vigna radiata) seed storage protein 8S globulin and its deposition was followed via im ...
... storage vacuoles (PSVs). Multiple mechanisms have been proposed to be responsible for transporting storage proteins to PSVs in developing seeds. In this study, a specific antibody was raised against the mung bean (Vigna radiata) seed storage protein 8S globulin and its deposition was followed via im ...
Cytokinesis
Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.