Convergence and Extension Movements During Vertebrate
... primitive streak of the avian gastrulae (Wei and Mikawa, 2000), the orientation of mitotic spindle is biased along the anteroposterior axis. In zebrafish, oriented cell division has also been observed during the C&E movements of the neuroepithelial progenitor cells (Concha and Adams, 1998; Gong et a ...
... primitive streak of the avian gastrulae (Wei and Mikawa, 2000), the orientation of mitotic spindle is biased along the anteroposterior axis. In zebrafish, oriented cell division has also been observed during the C&E movements of the neuroepithelial progenitor cells (Concha and Adams, 1998; Gong et a ...
AGING Dr Aubrey de Grey Chief Science Officer
... Mitochondrial mutations Allotopic expression of 13 proteins Intracellular junk Extracellular junk Extracellular crosslinks ...
... Mitochondrial mutations Allotopic expression of 13 proteins Intracellular junk Extracellular junk Extracellular crosslinks ...
A Flow Cytometry Approach to Detect In vivo Chromatin Compaction
... Evaluation of chromatin condensation is an important and stimulating topic. Cells, based on their conditions, can be divided into living or dead cells. For a long time chromosome banding pattern had been used to analyze the chromatin condensation [4]. This method depends much on chromosome separatio ...
... Evaluation of chromatin condensation is an important and stimulating topic. Cells, based on their conditions, can be divided into living or dead cells. For a long time chromosome banding pattern had been used to analyze the chromatin condensation [4]. This method depends much on chromosome separatio ...
ATCC® EnTEriC DisEAsE rEsEArCh MATEriAls
... The ATCC® Genuine Nucleics collection represents the largest and most diverse array of both cellular and microbiological genomic, synthetic, and certified reference materials available worldwide. Each preparation is isolated or synthetically derived under aseptic conditions to prevent cross-contamin ...
... The ATCC® Genuine Nucleics collection represents the largest and most diverse array of both cellular and microbiological genomic, synthetic, and certified reference materials available worldwide. Each preparation is isolated or synthetically derived under aseptic conditions to prevent cross-contamin ...
a Gene Encoding a Putative Signal Transduction Receptor in
... cells exchange pronuclei and fertilization occurs when the migratory nucleus from the mating partner fuses with the stationary pronucleus. The zygotic nuclei undergo two postzygotic mitoses to produce four nuclei in each cell, two of which develop into new macronuclei (macronuclear anlagen) and two ...
... cells exchange pronuclei and fertilization occurs when the migratory nucleus from the mating partner fuses with the stationary pronucleus. The zygotic nuclei undergo two postzygotic mitoses to produce four nuclei in each cell, two of which develop into new macronuclei (macronuclear anlagen) and two ...
Severe osmotic compression triggers a slowdown of
... Crz1p, and Mig1p (Fig. 4 and Movies S4, S5, and S6). Yap1p is involved in the oxidative stress response and contains a nuclear localization signal but is constantly exported from the nucleus by Crm1p (38). The addition of 300 μM hydrogen peroxide (H2O2) triggers oxidation of the cysteine residues in ...
... Crz1p, and Mig1p (Fig. 4 and Movies S4, S5, and S6). Yap1p is involved in the oxidative stress response and contains a nuclear localization signal but is constantly exported from the nucleus by Crm1p (38). The addition of 300 μM hydrogen peroxide (H2O2) triggers oxidation of the cysteine residues in ...
Activation of the Protein Kinase Akt/PKB by the
... The maintenance of structural and functional integrity of epithelia requires highly dynamic cellto-cell and cell-to-matrix interactions, which are mediated by adhesion mechanisms involving different types of cell-surface receptors. Among them, cadherins and integrins play a major role, as they are a ...
... The maintenance of structural and functional integrity of epithelia requires highly dynamic cellto-cell and cell-to-matrix interactions, which are mediated by adhesion mechanisms involving different types of cell-surface receptors. Among them, cadherins and integrins play a major role, as they are a ...
AP Biology, Chapter 28 Protists Living Small 28.1 Most eukaryotes
... Structural and Functional Diversity in Protists 1. Explain why protistan cells are not analogous to a single cell from a multicellular organism. Multicellular cells are specialized Many protistan cell show elaborate adaptations for performing all life functions 2. Describe the general protistan life ...
... Structural and Functional Diversity in Protists 1. Explain why protistan cells are not analogous to a single cell from a multicellular organism. Multicellular cells are specialized Many protistan cell show elaborate adaptations for performing all life functions 2. Describe the general protistan life ...
Limbal stem cell deficiency and its management
... Firstly, the corneal epithelium is renewed by stem cells located at the limbus, the socalled limbal stem cells. Secondly, the limbus also acts as a barrier preventing the conjunctival epithelium and its blood vessels from encroaching on to the corneal surface. When the limbal stem cells become defic ...
... Firstly, the corneal epithelium is renewed by stem cells located at the limbus, the socalled limbal stem cells. Secondly, the limbus also acts as a barrier preventing the conjunctival epithelium and its blood vessels from encroaching on to the corneal surface. When the limbal stem cells become defic ...
Cell Membrane Quiz 2013
... a. plants c. bacteria b. animals d. all of the above ____ 5. Which of the following is a function of the cytoskeleton? a. helps a cell keep its shape c. surrounds the cell b. contains DNA d. helps make proteins ____ 6. You will NOT find a cell wall in which of these kinds of organisms? a. plants c. ...
... a. plants c. bacteria b. animals d. all of the above ____ 5. Which of the following is a function of the cytoskeleton? a. helps a cell keep its shape c. surrounds the cell b. contains DNA d. helps make proteins ____ 6. You will NOT find a cell wall in which of these kinds of organisms? a. plants c. ...
Meiotic sex chromosome inactivation
... Following spermatogonial divisions, germ cells enter meiosis. During the earliest meiotic substage, leptotene, when the DNA double-strand breaks (DSBs) that initiate meiotic recombination are formed, the X and Y chromosomes are still transcriptionally active, and remain so throughout zygotene (Fig. ...
... Following spermatogonial divisions, germ cells enter meiosis. During the earliest meiotic substage, leptotene, when the DNA double-strand breaks (DSBs) that initiate meiotic recombination are formed, the X and Y chromosomes are still transcriptionally active, and remain so throughout zygotene (Fig. ...
Cell A.
... and DAG from the cell membrane. IP3 then binds to a ligand-gated ion channel on the ER causing the release of calcium ions, which bind to calmodulin & causes the cytoskeleton to change shape. 8.The binding of a sperm to an egg initiates a G protein pathway that releases IP3 & DAG. This releases Ca2+ ...
... and DAG from the cell membrane. IP3 then binds to a ligand-gated ion channel on the ER causing the release of calcium ions, which bind to calmodulin & causes the cytoskeleton to change shape. 8.The binding of a sperm to an egg initiates a G protein pathway that releases IP3 & DAG. This releases Ca2+ ...
pdf, 1.1 MB - The Nebenführ Lab
... To test whether this distribution of Golgi stacks is truly random we have correlated the number of Golgi stacks in different regions of the cells with the available volume of cytoplasm. For this purpose, cytoplasm was defined as the area covered by the combined fluorescence of our GFP-marker, which ...
... To test whether this distribution of Golgi stacks is truly random we have correlated the number of Golgi stacks in different regions of the cells with the available volume of cytoplasm. For this purpose, cytoplasm was defined as the area covered by the combined fluorescence of our GFP-marker, which ...
Plasmablast and Plasma Cell Production and Distribution in Trout
... were washed with PBS before the membrane was removed from the apparatus. The membrane was gently wiped (Kimwipes; Kimberly-Clark, Roswell, GA) to remove any cellular debris still attached to the membrane, then blocked by the addition of the blocking solution for 1 h. Biotinylated mAb 1–14 (0.5 g/ml ...
... were washed with PBS before the membrane was removed from the apparatus. The membrane was gently wiped (Kimwipes; Kimberly-Clark, Roswell, GA) to remove any cellular debris still attached to the membrane, then blocked by the addition of the blocking solution for 1 h. Biotinylated mAb 1–14 (0.5 g/ml ...
Isolation of a novel population of multipotent stem cells
... endothelial marker and CD56 (NCAM) neural adherent marker were used . Using these cell surface markers of stem cells, cytometry was performed on a FACS Calibur with sorter (BD Biosciences). These immunophenotypic markers with Cellquest software expressed the stem cells characteristics. FACS analyses ...
... endothelial marker and CD56 (NCAM) neural adherent marker were used . Using these cell surface markers of stem cells, cytometry was performed on a FACS Calibur with sorter (BD Biosciences). These immunophenotypic markers with Cellquest software expressed the stem cells characteristics. FACS analyses ...
Cell Wall Polysaccharides are Mislocalized to the Vacuole in
... freeze-substituted echidna and wild-type seeds examined using light microscopy (Fig. 1C; Gendre et al. 2013). During mucilage secretion, wild-type seed coat cells change from typical parenchymous cells to being dominated by a large deposit of polysaccharide on the apical face of the epidermal cells ...
... freeze-substituted echidna and wild-type seeds examined using light microscopy (Fig. 1C; Gendre et al. 2013). During mucilage secretion, wild-type seed coat cells change from typical parenchymous cells to being dominated by a large deposit of polysaccharide on the apical face of the epidermal cells ...
Comparison of an immortalized human corneal epithelial cell line
... blindness, is a sight threatening ocular infection often requiring a specific and prompt laboratory diagnosis [1]. A variety of techniques have been employed for the rapid diagnosis of HSK [2–5]. Isolation of Herpes simplex virus1(HSV-1) in culture provides the most reliable and specific method and ...
... blindness, is a sight threatening ocular infection often requiring a specific and prompt laboratory diagnosis [1]. A variety of techniques have been employed for the rapid diagnosis of HSK [2–5]. Isolation of Herpes simplex virus1(HSV-1) in culture provides the most reliable and specific method and ...
Use of the BacTiter-Glo™ Microbial Cell Viability Assay to Study
... Similarly, RLU that result from the BacTiter-Glo™ Assay can be converted to bacterial cell numbers with a bacterial calibration curve. To obtain this curve, a bacterial suspension of an E. coli wild-type strain that had been grown for 38 hours without aeration in tryptone broth (TB; 1% tryptone, 0.5 ...
... Similarly, RLU that result from the BacTiter-Glo™ Assay can be converted to bacterial cell numbers with a bacterial calibration curve. To obtain this curve, a bacterial suspension of an E. coli wild-type strain that had been grown for 38 hours without aeration in tryptone broth (TB; 1% tryptone, 0.5 ...
The role of fibroblast growth factor in early Xenopus development
... much appears to happen for the first few hours, the explants round up with their blastocoelic surface inside and the cells continue to cleave just like untreated explants. However, it is the first 90min or so of exposure that are critical. After this time, the FGF can be withdrawn without affecting ...
... much appears to happen for the first few hours, the explants round up with their blastocoelic surface inside and the cells continue to cleave just like untreated explants. However, it is the first 90min or so of exposure that are critical. After this time, the FGF can be withdrawn without affecting ...
here - The Nebenführ Lab
... cells in preparation for cell division (Katsuta et al. 1990) but play little role in interphase TVS. The different overall arrangements of TVS during the cell cycle have been described in great detail (Kumagai and Hasezawa 2001, Kutsuna and Hasezawa 2002). However, TVS are remarkably dynamic structu ...
... cells in preparation for cell division (Katsuta et al. 1990) but play little role in interphase TVS. The different overall arrangements of TVS during the cell cycle have been described in great detail (Kumagai and Hasezawa 2001, Kutsuna and Hasezawa 2002). However, TVS are remarkably dynamic structu ...
Mitosis
Mitosis is a part of the cell cycle in which chromosomes in a cell nucleus are separated into two identical sets of chromosomes, each in its own nucleus. In general, mitosis (division of the nucleus) is often followed by cytokinesis, which divides the cytoplasm, organelles and cell membrane into two new cells containing roughly equal shares of these cellular components. Mitosis and cytokinesis together define the mitotic (M) phase of an animal cell cycle—the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell.The process of mitosis is divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to fibers that pull one copy of each chromosome to opposite sides of the cell. The result is two genetically identical daughter nuclei. The cell may then divide by cytokinesis to produce two daughter cells. Producing three or more daughter cells instead of normal two is a mitotic error called tripolar mitosis or multipolar mitosis (direct cell triplication / multiplication). Other errors during mitosis can induce apoptosis (programmed cell death) or cause mutations. Certain types of cancer can arise from such mutations.Mitosis occurs only in eukaryotic cells and the process varies in different organisms. For example, animals undergo an ""open"" mitosis, where the nuclear envelope breaks down before the chromosomes separate, while fungi undergo a ""closed"" mitosis, where chromosomes divide within an intact cell nucleus. Furthermore, most animal cells undergo a shape change, known as mitotic cell rounding, to adopt a near spherical morphology at the start of mitosis. Prokaryotic cells, which lack a nucleus, divide by a different process called binary fission.