MICROSCOPIC AND FUNCTIONAL STUDIES ON
... from cell membrane (Figure 2). Microvesicles are also called as ectosomes (Stein and Luzio. 1991) and microparticles (Mackman. 2009) in literature. Recognition between exosomes and microvesicles is difficult because size ranges of these two type of vesicles partially overlap. In many cases their com ...
... from cell membrane (Figure 2). Microvesicles are also called as ectosomes (Stein and Luzio. 1991) and microparticles (Mackman. 2009) in literature. Recognition between exosomes and microvesicles is difficult because size ranges of these two type of vesicles partially overlap. In many cases their com ...
- Wiley Online Library
... family and biogenesis component PilQ and the twitching motility/pilus retraction protein PilT leads to the expression of type IV pili, which fail to reach the cell surface and remain localized inside tubulovesicular membrane protrusions of bacteria. We suggest that tubular appendages of bacteria cou ...
... family and biogenesis component PilQ and the twitching motility/pilus retraction protein PilT leads to the expression of type IV pili, which fail to reach the cell surface and remain localized inside tubulovesicular membrane protrusions of bacteria. We suggest that tubular appendages of bacteria cou ...
Diacylglycerol kinases - University of Toronto Mississauga
... domain in DGKy has been shown to bind weakly and nonselectively to phosphatidylinositols [33]. Type II DGKs also have sterile alpha motifs (SAM domain) at their carboxy termini. Nagaya et al. [21] demonstrated that the SAM domain of DGKy helped localize it to the endoplasmic reticulum, and this doma ...
... domain in DGKy has been shown to bind weakly and nonselectively to phosphatidylinositols [33]. Type II DGKs also have sterile alpha motifs (SAM domain) at their carboxy termini. Nagaya et al. [21] demonstrated that the SAM domain of DGKy helped localize it to the endoplasmic reticulum, and this doma ...
Caveolae as potential macromolecule trafficking
... non-coated plasma membrane vesicles or invaginations within the alveolar epithelial type I cell has long been recognised. The putative function of these vesicles in macromolecule transport remains the focus of research in both pulmonary physiology and pharmaceutical science disciplines. These vesicl ...
... non-coated plasma membrane vesicles or invaginations within the alveolar epithelial type I cell has long been recognised. The putative function of these vesicles in macromolecule transport remains the focus of research in both pulmonary physiology and pharmaceutical science disciplines. These vesicl ...
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... An essential step in the determination of structure-function relationships at a molecular level is to investigate the intrinsic properties of isolated proteins, via molecular biology, biochemical and structural approaches [1–3]. However, it is also necessary to study protein function in an integrate ...
... An essential step in the determination of structure-function relationships at a molecular level is to investigate the intrinsic properties of isolated proteins, via molecular biology, biochemical and structural approaches [1–3]. However, it is also necessary to study protein function in an integrate ...
-ATPases:
... of cation pumps that (a) form an aspartyl phosphate reaction cycle intermediate, (b) are inhibited by vanadate, and (c) share a common domain organization (12, 73, 80, 106). Other ATPases belonging to this family include the fungal plasma membrane H+-ATPases; the animal Na+/K+-ATPase; the gastric H+ ...
... of cation pumps that (a) form an aspartyl phosphate reaction cycle intermediate, (b) are inhibited by vanadate, and (c) share a common domain organization (12, 73, 80, 106). Other ATPases belonging to this family include the fungal plasma membrane H+-ATPases; the animal Na+/K+-ATPase; the gastric H+ ...
How does photosystem 2 split water?
... which a high resolution three-dimensional structure is available 2. Although useful, this comparison is restricted, as phototrophic purple bacteria do not split water and their subunit composition is much simpler than that for PS2. In PS2, the chlorophyll-binding proteins CP43 (PsbC*) and CP47 (PsbB ...
... which a high resolution three-dimensional structure is available 2. Although useful, this comparison is restricted, as phototrophic purple bacteria do not split water and their subunit composition is much simpler than that for PS2. In PS2, the chlorophyll-binding proteins CP43 (PsbC*) and CP47 (PsbB ...
J., Schornack, S., Spallek, T., Geldner, N., Chory
... expansion by fusion to the plasma membrane at the collar region followed by sorting at the neckband (An et al., 2006a; Meyer et al., 2009). Alternatively, the EHM could form directly from fusing secretory vesicles since ER/Golgi-type vesicles accumulate at the penetration site of the growing haustor ...
... expansion by fusion to the plasma membrane at the collar region followed by sorting at the neckband (An et al., 2006a; Meyer et al., 2009). Alternatively, the EHM could form directly from fusing secretory vesicles since ER/Golgi-type vesicles accumulate at the penetration site of the growing haustor ...
Co-translational Targeting and Translocation of the Amino Terminus
... of both cytosolic and ER-lumenal ATPase chaperones (14, 16, 17). The lumenal chaperone BiP (also known as Kar2 and Grp78) plays a critical role in yeast translocation. Both temperature-sensitive mutations in BiP and decreased expression of BiP cause translocation defects in vivo (18, 19). Proteolipo ...
... of both cytosolic and ER-lumenal ATPase chaperones (14, 16, 17). The lumenal chaperone BiP (also known as Kar2 and Grp78) plays a critical role in yeast translocation. Both temperature-sensitive mutations in BiP and decreased expression of BiP cause translocation defects in vivo (18, 19). Proteolipo ...
Rapid Changes in Synaptic Vesicle Cytochemistry
... ABstrAct Sympathetic neurons taken from rat superior cervical ganglia and grown in culture acquire cholinergic function under certain conditions. These cholinergic sympathetic neurons, however, retain a number of adrenergic properties, including the enzymes involved in the synthesis of norepinephrin ...
... ABstrAct Sympathetic neurons taken from rat superior cervical ganglia and grown in culture acquire cholinergic function under certain conditions. These cholinergic sympathetic neurons, however, retain a number of adrenergic properties, including the enzymes involved in the synthesis of norepinephrin ...
Early and late endosomal compartments of Entamoeba histolytica
... importance of the E. histolytica endo-lysosomal and secretory systems in nutrition and invasion of the organism, relatively little is known about the molecular factors governing these systems including the associated proteins which may regulate endocytic or secretory function. In other eukaryotes, t ...
... importance of the E. histolytica endo-lysosomal and secretory systems in nutrition and invasion of the organism, relatively little is known about the molecular factors governing these systems including the associated proteins which may regulate endocytic or secretory function. In other eukaryotes, t ...
Transmembrane Fragment Structures of Amyloid Precursor Protein
... and micelle environments. As shown in Figure 6 (MSD), waters closer to the POPC bilayer interface show smaller MSD values compared to those close to the DPC micelle interface. Given these combined results, it appears that restrained waters at the POPC interface (1) reduce the lipid/solvent interfaci ...
... and micelle environments. As shown in Figure 6 (MSD), waters closer to the POPC bilayer interface show smaller MSD values compared to those close to the DPC micelle interface. Given these combined results, it appears that restrained waters at the POPC interface (1) reduce the lipid/solvent interfaci ...
Summary - Universität Tübingen
... are formed de novo during interphase into an intact nuclear envelope, which is also the exclusive assembly mode in organisms undergoing a closed mitosis as Saccharomyces cerevisiae. Particularly, how NPCs integrate both, in the growing nuclear envelope at the end of mitosis and the closed nuclear en ...
... are formed de novo during interphase into an intact nuclear envelope, which is also the exclusive assembly mode in organisms undergoing a closed mitosis as Saccharomyces cerevisiae. Particularly, how NPCs integrate both, in the growing nuclear envelope at the end of mitosis and the closed nuclear en ...
Regulators of Lysosome Function and Dynamics in Caenorhabditis
... genes that correspond to the 20 mutations. We included the hypomorphic cup-5(ar465) allele in this analysis because we had originally identified this mutant on the basis of a similar phenotype in Pmyo-3::ssGFP worms (Fares and Greenwald 2001a). Our analysis indicates that we indeed identified several ...
... genes that correspond to the 20 mutations. We included the hypomorphic cup-5(ar465) allele in this analysis because we had originally identified this mutant on the basis of a similar phenotype in Pmyo-3::ssGFP worms (Fares and Greenwald 2001a). Our analysis indicates that we indeed identified several ...
Annexins: multifunctional components of growth and adaptation
... function. This is supported by the observation that both plant and animal annexins bind to a range of negatively charged phospholipids in addition to phosphatidylserine, including phosphatidylinositol, phosphatidic acid, and malonaldehyde-conjugated lipids (Blackbourn et al., 1991; Balasubramanian e ...
... function. This is supported by the observation that both plant and animal annexins bind to a range of negatively charged phospholipids in addition to phosphatidylserine, including phosphatidylinositol, phosphatidic acid, and malonaldehyde-conjugated lipids (Blackbourn et al., 1991; Balasubramanian e ...
Regulators of Lysosome Function and Dynamics in Caenorhabditis
... al. 2012). Primary lysosomes that are formed are thought to undergo cycles of homotypic fusion with each other using the HOPS complex and SNARES, and fission reactions that yield mature lysosomes that can fuse with late endosomes (WARD et al. 2000; WANG et al. 2003). In addition to all of these memb ...
... al. 2012). Primary lysosomes that are formed are thought to undergo cycles of homotypic fusion with each other using the HOPS complex and SNARES, and fission reactions that yield mature lysosomes that can fuse with late endosomes (WARD et al. 2000; WANG et al. 2003). In addition to all of these memb ...
SODIUM-COUPLED TRANSPORTERS FOR KREBS CYCLE
... ABSTRACT Krebs cycle intermediates such as succinate, citrate, and α-ketoglutarate are transferred across plasma membranes of cells by secondary active transporters that couple the downhill movement of sodium to the concentrative uptake of substrate. Several transporters have been identified in isol ...
... ABSTRACT Krebs cycle intermediates such as succinate, citrate, and α-ketoglutarate are transferred across plasma membranes of cells by secondary active transporters that couple the downhill movement of sodium to the concentrative uptake of substrate. Several transporters have been identified in isol ...
Presequence-Independent Manner Transported by the TIM23
... The majority of multispanning inner mitochondrial membrane proteins utilize internal targeting signals, which direct them to the carrier translocase (TIM22 complex), for their import. MPV17 and its Saccharomyces cerevisiae orthologue Sym1 are multispanning inner membrane proteins of unknown function ...
... The majority of multispanning inner mitochondrial membrane proteins utilize internal targeting signals, which direct them to the carrier translocase (TIM22 complex), for their import. MPV17 and its Saccharomyces cerevisiae orthologue Sym1 are multispanning inner membrane proteins of unknown function ...
Electron tomography of plant thylakoid membranes
... Electron tomography (ET) makes it possible to image biological specimens in three dimensions in the electron microscope on the nanometre scale (for recent reviews, see Bárcena and Koster, 2009; Pierson et al., 2009; Ben-Harush et al., 2010; Tocheva et al., 2010). 3D information is collected by reco ...
... Electron tomography (ET) makes it possible to image biological specimens in three dimensions in the electron microscope on the nanometre scale (for recent reviews, see Bárcena and Koster, 2009; Pierson et al., 2009; Ben-Harush et al., 2010; Tocheva et al., 2010). 3D information is collected by reco ...
Golgi-targeting sequence of p230 - Journal of Cell Science
... p230 is a peripheral membrane protein that is localised on the cytoplasmic side of TGN membranes, as well as a subpopulation of non-clathrin-coated vesicles budding from the TGN (Gleeson et al., 1996). As p230 recycles between the cytosol and the membranes of the TGN, there must be a mechanism to ta ...
... p230 is a peripheral membrane protein that is localised on the cytoplasmic side of TGN membranes, as well as a subpopulation of non-clathrin-coated vesicles budding from the TGN (Gleeson et al., 1996). As p230 recycles between the cytosol and the membranes of the TGN, there must be a mechanism to ta ...
Insights into the Role of Specific Lipids in the
... and signal transduction (Simons and Ikonen, 1997; Brown and London, 1998; Keller and Simons, 1998; Simons and Toomre, 2000; Simons and Vaz, 2004; Hancock, 2006). Similar microdomains in the PM of yeast (Saccharomyces cerevisiae) cells, enriched in ergosterol, have also been reported (Bagnat et al., ...
... and signal transduction (Simons and Ikonen, 1997; Brown and London, 1998; Keller and Simons, 1998; Simons and Toomre, 2000; Simons and Vaz, 2004; Hancock, 2006). Similar microdomains in the PM of yeast (Saccharomyces cerevisiae) cells, enriched in ergosterol, have also been reported (Bagnat et al., ...
REVIEWS Structural insights into the function of the
... in FAD binding to flavoproteins. The structure is rotated ~180 ~ about the vertical relative to (a) to of wild-type Rab2~ Further exbring the FAD-binding groove and the ~(~13unit (yellow) and the remnant GXG motif (red) into view. The orange strands flanking the 13(~i3unit are part of the extended s ...
... in FAD binding to flavoproteins. The structure is rotated ~180 ~ about the vertical relative to (a) to of wild-type Rab2~ Further exbring the FAD-binding groove and the ~(~13unit (yellow) and the remnant GXG motif (red) into view. The orange strands flanking the 13(~i3unit are part of the extended s ...
SNARE (protein)
SNARE proteins (an acronym derived from ""SNAP (Soluble NSF Attachment Protein) REceptor"") are a large protein superfamily consisting of more than 60 members in yeast and mammalian cells. The primary role of SNARE proteins is to mediate vesicle fusion, that is, the fusion of vesicles with their target membrane bound compartments (such as a lysosome). The best studied SNAREs are those that mediate docking of synaptic vesicles with the presynaptic membrane in neurons. These SNAREs are the targets of the bacterial neurotoxins responsible for botulism and tetanus.