Subcellular Trafficking of Mammalian Lysosomal Proteins: An
... sites (i.e., to redirect the protein to the PM), an unconventional dileucine signal located in a cytosolic loop (SLLKGRQGIY) must be mutated simultaneously with a tryptophan and glutamic acid (WE) motif located in the C-terminal tail [33]. However, not all atypical signals revolve around tyrosine or ...
... sites (i.e., to redirect the protein to the PM), an unconventional dileucine signal located in a cytosolic loop (SLLKGRQGIY) must be mutated simultaneously with a tryptophan and glutamic acid (WE) motif located in the C-terminal tail [33]. However, not all atypical signals revolve around tyrosine or ...
An immunoassay for atrazine using tunable immunosorbent Jae-Young Kim, Ashok Mulchandani,
... in which the interchaining interaction and atrazinebinding functions are engineered independently. The availability of genetic engineering technology now provides the possibility to specifically design immunosorbents with tunable properties that can be used to selectively detect atrazine from dilute ...
... in which the interchaining interaction and atrazinebinding functions are engineered independently. The availability of genetic engineering technology now provides the possibility to specifically design immunosorbents with tunable properties that can be used to selectively detect atrazine from dilute ...
Chapter 4 Calsequestrin - Department of Molecular Physiology and
... cellulose, hydroxylapatite, and Sephadex. It was extremely acidic; it contained about 37% acidic amino acid residues and only 7% basic residues. It bound nearly 1000 nmole of Ca2+/mg, forming an insoluble Ca 2+calsequestrin complex in the process. Calculation of the concentration of the protein in t ...
... cellulose, hydroxylapatite, and Sephadex. It was extremely acidic; it contained about 37% acidic amino acid residues and only 7% basic residues. It bound nearly 1000 nmole of Ca2+/mg, forming an insoluble Ca 2+calsequestrin complex in the process. Calculation of the concentration of the protein in t ...
The Qualitative Effects of Resveratrol ... Administration on the Gluteus Complex ...
... VAN DER SPUY, W. J. & PRETORIUS, E. The qualitative effects of resveratrol and coenzyme Q10 administration on the gluteus complex muscle morphology of SJL/J mice with dysferlinopathy. Int. J. Morphol., 29(3):876-884, 2011. SUMMARY: Dysferlinopathy is a form of muscular dystrophy affecting muscles of ...
... VAN DER SPUY, W. J. & PRETORIUS, E. The qualitative effects of resveratrol and coenzyme Q10 administration on the gluteus complex muscle morphology of SJL/J mice with dysferlinopathy. Int. J. Morphol., 29(3):876-884, 2011. SUMMARY: Dysferlinopathy is a form of muscular dystrophy affecting muscles of ...
Anterior PAR proteins function during cytokinesis and
... and Hyman, 2004; Macara, 2004; Shivas et al., 2010], but their function during cell division remains elusive. Cell polarity is naturally coupled with many events that occur during cell division. As a tissue proliferates and develops, each cell must maintain its polarization state upon each division ...
... and Hyman, 2004; Macara, 2004; Shivas et al., 2010], but their function during cell division remains elusive. Cell polarity is naturally coupled with many events that occur during cell division. As a tissue proliferates and develops, each cell must maintain its polarization state upon each division ...
ENS’06 FUSION PHAGE AS A BIOSELECTIVE NANOMATERIAL: EVOLUTION OF THE CONCEPT
... its conformation to accommodate to various distinctly different forms of the phage and its precursors: phage filament, intermediate particle (I-form), spheroid (Sform), and membrane-bound form. This structural flexibility of the major coat protein is determined by its unique architecture, which is s ...
... its conformation to accommodate to various distinctly different forms of the phage and its precursors: phage filament, intermediate particle (I-form), spheroid (Sform), and membrane-bound form. This structural flexibility of the major coat protein is determined by its unique architecture, which is s ...
CONTRIBUTION OF DYSFERLIN-CONTAINING
... present in the basal lamina. The intracellular linkage to F-actin is mediated by dystrophin, a >400 kDa protein that contains an N-terminal actin binding domain, a central rod domain and a C-terminal domain that interacts with β-dystroglycan within the plasma membrane [10]. Dystroglycan is a membran ...
... present in the basal lamina. The intracellular linkage to F-actin is mediated by dystrophin, a >400 kDa protein that contains an N-terminal actin binding domain, a central rod domain and a C-terminal domain that interacts with β-dystroglycan within the plasma membrane [10]. Dystroglycan is a membran ...
Full-Text PDF
... two different α-helices (GxGT-(38–40 residues)-D/E), but typically some of them are non-functional. In plant annexins the Ca2+ -binding motif is highly conserved in repeat I, generally lost in repeats II and III, and only moderately conserved in repeat IV [3,13]. For example, Arabidopsis ANNAT1 and ...
... two different α-helices (GxGT-(38–40 residues)-D/E), but typically some of them are non-functional. In plant annexins the Ca2+ -binding motif is highly conserved in repeat I, generally lost in repeats II and III, and only moderately conserved in repeat IV [3,13]. For example, Arabidopsis ANNAT1 and ...
WW Domains Provide a Platform for the
... during 25 min with a total run time of 45 min. Data were analyzed in batch using the Mascot search engine (57), and proteins were considered “hits” if two independent peptides or a single peptide with a Mascot score of 50 or higher was found. Protein hits were converted to gene identifiers (GeneIDs) ...
... during 25 min with a total run time of 45 min. Data were analyzed in batch using the Mascot search engine (57), and proteins were considered “hits” if two independent peptides or a single peptide with a Mascot score of 50 or higher was found. Protein hits were converted to gene identifiers (GeneIDs) ...
Linköping University Post Print
... [4]. However, in 1998, we discovered that the aspartic protease cathepsin D was redistributed from lysosomes to the cytosol upon oxidative stress-induced apoptotic cell death [5] (Figure 1). Cathepsin D was the first identified lysosomal protein with apoptogenic properties, but the subcellular locat ...
... [4]. However, in 1998, we discovered that the aspartic protease cathepsin D was redistributed from lysosomes to the cytosol upon oxidative stress-induced apoptotic cell death [5] (Figure 1). Cathepsin D was the first identified lysosomal protein with apoptogenic properties, but the subcellular locat ...
Ch. 2 - 서울대 Biointelligence lab
... ♦ Be synthesized by and localized within the presynaptic neuron, and stored in the presynaptic terminal. ♦ Be released by the presynaptic neuron when action potentials invade and depolarize the terminal. ♦ Contain receptors that are specific for the substance. ♦ When applied to the postsynaptic cell ...
... ♦ Be synthesized by and localized within the presynaptic neuron, and stored in the presynaptic terminal. ♦ Be released by the presynaptic neuron when action potentials invade and depolarize the terminal. ♦ Contain receptors that are specific for the substance. ♦ When applied to the postsynaptic cell ...
Type III secretion: The bacteria-eukaryotic cell
... first injectisome, from Salmonella enterica, was reported in 1998 [1]. Afterwards, EM studies allowed the visualization of the complete injectisomes from Shigella flexneri [2] and EPEC [3]. Recently, cryo-EM analyses allowed the visualization at 17 Å resolution of the S. enterica injectisome [4]. All ...
... first injectisome, from Salmonella enterica, was reported in 1998 [1]. Afterwards, EM studies allowed the visualization of the complete injectisomes from Shigella flexneri [2] and EPEC [3]. Recently, cryo-EM analyses allowed the visualization at 17 Å resolution of the S. enterica injectisome [4]. All ...
enterocolitica Yersinia Type III Secretion System of Properties of the
... particularly marked in their C-terminal halves. Deletion mutagenesis (20) and limited proteolysis (4, 43) have shown that this domain participates in oligomerization and pore formation, while the N terminus is probably involved in systemspecific interactions (12, 49). A small nonconserved domain C t ...
... particularly marked in their C-terminal halves. Deletion mutagenesis (20) and limited proteolysis (4, 43) have shown that this domain participates in oligomerization and pore formation, while the N terminus is probably involved in systemspecific interactions (12, 49). A small nonconserved domain C t ...
The Activity of a Developmentally Regulated Cysteine Proteinase Is
... priming the template with oligo(dT)20. NH2-terminal amino acid sequencing of the purified mature proteinase, in conjunction with codon usage knowledge in Giardia (32, 33), allowed the design of a moderately degenerate primer (5⬘-GA(C/T)GG(A/C/G)AT(T/C)GG(A/C/G)AA(T/C)GC(A/C/G)(T/A)(C/G)(C/A/G)CA(G/A ...
... priming the template with oligo(dT)20. NH2-terminal amino acid sequencing of the purified mature proteinase, in conjunction with codon usage knowledge in Giardia (32, 33), allowed the design of a moderately degenerate primer (5⬘-GA(C/T)GG(A/C/G)AT(T/C)GG(A/C/G)AA(T/C)GC(A/C/G)(T/A)(C/G)(C/A/G)CA(G/A ...
Protein targeting, translocation and Escherichia coli Proteomic analysis of substrate-pathway relationships
... minimizes the energetic cost for lipids to exist in a polar, aqueous environment. The hydrophobic core region makes the bilayer impermeable by blocking passage of polar molecules. Membrane lipids have different properties depending on the chemical composition of their head and tail regions. The hea ...
... minimizes the energetic cost for lipids to exist in a polar, aqueous environment. The hydrophobic core region makes the bilayer impermeable by blocking passage of polar molecules. Membrane lipids have different properties depending on the chemical composition of their head and tail regions. The hea ...
Regulation of KNOLLE syntaxin - Journal of Cell Science
... proteins, interacts with the v-SNARE synaptobrevin on the vesicle membrane (Clague and Herrmann, 2000). There are numerous members of each SNARE protein family in yeast, animals and plants that have been implicated in diverse vesicle trafficking pathways between membrane compartments (for reviews on ...
... proteins, interacts with the v-SNARE synaptobrevin on the vesicle membrane (Clague and Herrmann, 2000). There are numerous members of each SNARE protein family in yeast, animals and plants that have been implicated in diverse vesicle trafficking pathways between membrane compartments (for reviews on ...
PDF Datastream - Brown Digital Repository
... Efforts to characterize proteins found in the outer membrane of gram-negative bacteria have been steadily increasing due to the promise of expanding our understanding of fundamental bacterial processes such as cell wall biogenesis as well as the promise of finding potential vaccine- or drug-targets ...
... Efforts to characterize proteins found in the outer membrane of gram-negative bacteria have been steadily increasing due to the promise of expanding our understanding of fundamental bacterial processes such as cell wall biogenesis as well as the promise of finding potential vaccine- or drug-targets ...
UNIT II - Elsevier Health
... the Na+-K+ pump is shown to provide an additional contribution to the resting potential. In this figure, there is continuous pumping of three sodium ions to the outside for each two potassium ions pumped to the inside of the membrane. The fact that more sodium ions are being pumped to the outside th ...
... the Na+-K+ pump is shown to provide an additional contribution to the resting potential. In this figure, there is continuous pumping of three sodium ions to the outside for each two potassium ions pumped to the inside of the membrane. The fact that more sodium ions are being pumped to the outside th ...
Clarinet (CLA-‐1), a novel active zone protein required for synaptic
... zone (Cases-‐Langhoff et al., 1996; Langnaese et al., 1996; Mukherjee et al., 2010). Although the core ...
... zone (Cases-‐Langhoff et al., 1996; Langnaese et al., 1996; Mukherjee et al., 2010). Although the core ...
Clarinet (CLA-‐1), a novel active zone protein required for
... zone (Cases-‐Langhoff et al., 1996; Langnaese et al., 1996; Mukherjee et al., 2010). Although the core ...
... zone (Cases-‐Langhoff et al., 1996; Langnaese et al., 1996; Mukherjee et al., 2010). Although the core ...
pdf file - John Innes Centre
... an excellent model system in which to explore, at the molecular level, the way that effector ligands control the interaction of a PII protein with its target. Deuridylylation of GlnK, as a consequence of ammonium shock, coincides with its membrane sequestration by AmtB (14). The E. coli AmtB-GlnK co ...
... an excellent model system in which to explore, at the molecular level, the way that effector ligands control the interaction of a PII protein with its target. Deuridylylation of GlnK, as a consequence of ammonium shock, coincides with its membrane sequestration by AmtB (14). The E. coli AmtB-GlnK co ...
The plant Golgi apparatus—Going with the flow
... vesicles carry these macromolecules to the plasma membrane [27,28]. Although synthesis of cellulose takes place on the plasma membrane, it also depends on the transport of membrane-bound enzymes, the cellulose synthases, from the Golgi to the plasma membrane. Certain glycosylation events are specifi ...
... vesicles carry these macromolecules to the plasma membrane [27,28]. Although synthesis of cellulose takes place on the plasma membrane, it also depends on the transport of membrane-bound enzymes, the cellulose synthases, from the Golgi to the plasma membrane. Certain glycosylation events are specifi ...
Effects of Single Amino Acid Substitution on the Biophysical
... displacement of the divalent cations, such as Mg2+ and Ca2+, which stabilize the LPS by binding to the anionic phosphate groups, then AMPs target the inner membrane and destroy the inner membrane by making holes or channels [5]. Initially, cationic peptides contact with the negatively charged lipid ...
... displacement of the divalent cations, such as Mg2+ and Ca2+, which stabilize the LPS by binding to the anionic phosphate groups, then AMPs target the inner membrane and destroy the inner membrane by making holes or channels [5]. Initially, cationic peptides contact with the negatively charged lipid ...
Biogenesis of proteins of the mitochondrial intermembrane space
... 1994; Young, Hoogenraad et al. 2003). Specific signals within the precursor proteins direct them to mitochondria and subsequently to their distinct subcompartment in the organelle. Sophisticated molecular machineries, named translocases, have evolved to mediate protein import and sorting and subsequ ...
... 1994; Young, Hoogenraad et al. 2003). Specific signals within the precursor proteins direct them to mitochondria and subsequently to their distinct subcompartment in the organelle. Sophisticated molecular machineries, named translocases, have evolved to mediate protein import and sorting and subsequ ...
OMPROT PP2 ver4 - Plant Physiology
... undergo fusion, fission and rapid movements, suggesting a dynamic interaction with components of the cytoskeleton (Sheahan et al., 2004; Sheahan et al., 2005; Logan, 2010). However, many of the specific proteins that mediate such processes remain unknown. While mitochondria do play a central role in ...
... undergo fusion, fission and rapid movements, suggesting a dynamic interaction with components of the cytoskeleton (Sheahan et al., 2004; Sheahan et al., 2005; Logan, 2010). However, many of the specific proteins that mediate such processes remain unknown. While mitochondria do play a central role in ...
SNARE (protein)
SNARE proteins (an acronym derived from ""SNAP (Soluble NSF Attachment Protein) REceptor"") are a large protein superfamily consisting of more than 60 members in yeast and mammalian cells. The primary role of SNARE proteins is to mediate vesicle fusion, that is, the fusion of vesicles with their target membrane bound compartments (such as a lysosome). The best studied SNAREs are those that mediate docking of synaptic vesicles with the presynaptic membrane in neurons. These SNAREs are the targets of the bacterial neurotoxins responsible for botulism and tetanus.