Ezrin: a protein requiring conformational activation to link
... transmembrane signaling pathways. The ERM (ezrinradixin-moesin) family of proteins, of which ezrin is the best studied member, play structural and regulatory roles in the assembly and stabilization of specialized plasma membrane domains. Ezrin and related molecules are concentrated in surface projec ...
... transmembrane signaling pathways. The ERM (ezrinradixin-moesin) family of proteins, of which ezrin is the best studied member, play structural and regulatory roles in the assembly and stabilization of specialized plasma membrane domains. Ezrin and related molecules are concentrated in surface projec ...
as PDF
... Durell et al. proposed a 3-D structural model of amyloid channels obtained from a computer simulation of the secondary structure of AβP(1–40) in membranes that showed the aggregation of 5- to 8-mers to form pore-like structures on the membranes (Durell et al., 1994). Jang et al. established a model ...
... Durell et al. proposed a 3-D structural model of amyloid channels obtained from a computer simulation of the secondary structure of AβP(1–40) in membranes that showed the aggregation of 5- to 8-mers to form pore-like structures on the membranes (Durell et al., 1994). Jang et al. established a model ...
the electron microscopic localization of
... are invariably closely applied to each other, unless they are separated by interpolated glial tendrils. Trujillo-Cen6z (1962) stressed that mere close apposition of axon surfaces is, however, of too general occurrence within the neuropile to indicate per se synaptic contact, but closer examination o ...
... are invariably closely applied to each other, unless they are separated by interpolated glial tendrils. Trujillo-Cen6z (1962) stressed that mere close apposition of axon surfaces is, however, of too general occurrence within the neuropile to indicate per se synaptic contact, but closer examination o ...
Interactions of liposomes and lipid-based carrier systems with blood
... desirable to employ well defined large unilamellar vesicles (LUVs) having a narrow size distribution because vesicle size affects both liposome stability and clearance rates [9,10]. Typically, we employ LUVs that have been prepared by an extrusion procedure through 100 nm pore-sized filters [11]. Th ...
... desirable to employ well defined large unilamellar vesicles (LUVs) having a narrow size distribution because vesicle size affects both liposome stability and clearance rates [9,10]. Typically, we employ LUVs that have been prepared by an extrusion procedure through 100 nm pore-sized filters [11]. Th ...
Cholesterol and nicotinic acetylcholine receptor
... have actually learned about this lipid since its “recent” bicentennial discovery – considering that the enzymatic machinery for sterol biosynthesis may have appeared on Earth between 2.7 and 2.4 billion years ago [19], after the emergence of oxygenic photosynthesis with the concomitant oxygenation o ...
... have actually learned about this lipid since its “recent” bicentennial discovery – considering that the enzymatic machinery for sterol biosynthesis may have appeared on Earth between 2.7 and 2.4 billion years ago [19], after the emergence of oxygenic photosynthesis with the concomitant oxygenation o ...
In-Cell Solid-State NMR - Institut de Biologie Physico
... Although in-cell solid-state NMR has benefited from recent developments such as MAS for high-resolution solid-state NMR and DNP for signal enhancement, the application of in-cell NMR to proteins also relies on one’s ability to overexpress these biomolecules to ensure that they are 1) concentrated en ...
... Although in-cell solid-state NMR has benefited from recent developments such as MAS for high-resolution solid-state NMR and DNP for signal enhancement, the application of in-cell NMR to proteins also relies on one’s ability to overexpress these biomolecules to ensure that they are 1) concentrated en ...
Bioelectric Phenomena
... convinced that the muscular contractions he observed in frog legs were due to some form of electrical energy emanating from the animal. On the other hand, Allesandro Volta, a professor of physics at the University of Padua, was convinced that the “electricity” described in Galvani’s experiments orig ...
... convinced that the muscular contractions he observed in frog legs were due to some form of electrical energy emanating from the animal. On the other hand, Allesandro Volta, a professor of physics at the University of Padua, was convinced that the “electricity” described in Galvani’s experiments orig ...
Cellular control of cholesterol
... particles from which lipids and cholesterol can be extracted. Fats are digested by lipases and solubilized by bile from the liver. The combination of lipases and bile converts fat into small miscelles that contain a mixture of lipids, triglycerides and cholesterol. Lipids and cholesterol were origin ...
... particles from which lipids and cholesterol can be extracted. Fats are digested by lipases and solubilized by bile from the liver. The combination of lipases and bile converts fat into small miscelles that contain a mixture of lipids, triglycerides and cholesterol. Lipids and cholesterol were origin ...
Introduction to biophysics
... y Cajal argued persuasively that nerve cells are discrete entities and that they communicate with one another by means of specialized contacts called synapses. The human brain is estimated to contain 100 billion neurons and several times as many supporting cells, called neuroglial cells. ...
... y Cajal argued persuasively that nerve cells are discrete entities and that they communicate with one another by means of specialized contacts called synapses. The human brain is estimated to contain 100 billion neurons and several times as many supporting cells, called neuroglial cells. ...
(1) Giycophorin was incorporated into large
... compared to the size of the particles, revealed by freeze-fracture electron microscopy on the glycophorin-containing bilayer and are discussed in the light of various types of lipid-protein interactions and protein aggregation state. Introduction The primary function of biological membranes is to fo ...
... compared to the size of the particles, revealed by freeze-fracture electron microscopy on the glycophorin-containing bilayer and are discussed in the light of various types of lipid-protein interactions and protein aggregation state. Introduction The primary function of biological membranes is to fo ...
Neutrophils injure cultured skeletal myotubes
... cytoplasm of myotubes. This hypothesis was based on the fact that neutrophils can release ROIs, RNIs, and lysosomal enzymes that may cause myotube membrane rupture and necrosis (reviewed in Refs. 4 and 28). Because previous investigators have reported that traumatic injury to cultured cells (e.g., n ...
... cytoplasm of myotubes. This hypothesis was based on the fact that neutrophils can release ROIs, RNIs, and lysosomal enzymes that may cause myotube membrane rupture and necrosis (reviewed in Refs. 4 and 28). Because previous investigators have reported that traumatic injury to cultured cells (e.g., n ...
Systemic methods for capturing protein–lipid interactions (PDF
... • 25% of the identified lipid interaction proteome is enriched in drug targets, while 12% of total human proteome is drugged. lipid probes may preferentially interact with proteins that can bind other small molecule ligands ...
... • 25% of the identified lipid interaction proteome is enriched in drug targets, while 12% of total human proteome is drugged. lipid probes may preferentially interact with proteins that can bind other small molecule ligands ...
Import of ADP/ATP Carrier into Mitochondria: Two Receptors Act in
... residues are found in at least 40% of the TPR sequences) and a predicted predominantly helical conformation. The TPR motif was found in proteins that are encoded by genes required for mitosis or RNA synthesis. The TPR gene family includes CDC23, CDC16, nuc2 +, bimA, SSN6, and SK13 (Sikorski et al., ...
... residues are found in at least 40% of the TPR sequences) and a predicted predominantly helical conformation. The TPR motif was found in proteins that are encoded by genes required for mitosis or RNA synthesis. The TPR gene family includes CDC23, CDC16, nuc2 +, bimA, SSN6, and SK13 (Sikorski et al., ...
LY-294002-inhibitable PI 3-kinase and regulation of baseline rates
... was increased to 30 M. At these times, channels have redistributed between closed, open, and blocked states (20). PDS were acquired at 10 M CDPC just before elevation of CDPC to 30 M. PDS were also acquired at 30 M CDPC during the last 2 min of the 2-min, 40-s pulse durations, thereby yielding t ...
... was increased to 30 M. At these times, channels have redistributed between closed, open, and blocked states (20). PDS were acquired at 10 M CDPC just before elevation of CDPC to 30 M. PDS were also acquired at 30 M CDPC during the last 2 min of the 2-min, 40-s pulse durations, thereby yielding t ...
The Biochemical Machinery of Plastid Envelope
... between the nucleus and the organelle. Envelope membranes at the border between plastids and the cytosol play a role in this coordination at least at two levels, by interacting with the plastid translation and transcription apparatus, and through the import of nuclear-encoded proteins. In plastids c ...
... between the nucleus and the organelle. Envelope membranes at the border between plastids and the cytosol play a role in this coordination at least at two levels, by interacting with the plastid translation and transcription apparatus, and through the import of nuclear-encoded proteins. In plastids c ...
Review Article GPCRs: Lipid-Dependent Membrane Receptors That
... stabilized by ligands such as inverse agonists [28, 29]. As mentioned above, the inherent conformational instability (flexibility) poses a problem for X-ray crystallography. This was taken care of by stabilizing the flexible regions of GPCRs (e.g., the third intracellular loop) using monoclonal anti ...
... stabilized by ligands such as inverse agonists [28, 29]. As mentioned above, the inherent conformational instability (flexibility) poses a problem for X-ray crystallography. This was taken care of by stabilizing the flexible regions of GPCRs (e.g., the third intracellular loop) using monoclonal anti ...
REVIEWS
... The acquisition of multidrug resistance is a serious impediment to improved healthcare. Multidrug resistance is most frequently due to active transporters that pump a broad spectrum of chemically distinct, cytotoxic molecules out of cells, including antibiotics, antimalarials, herbicides and cancer ...
... The acquisition of multidrug resistance is a serious impediment to improved healthcare. Multidrug resistance is most frequently due to active transporters that pump a broad spectrum of chemically distinct, cytotoxic molecules out of cells, including antibiotics, antimalarials, herbicides and cancer ...
Holding it all together? Candidate proteins for the plant Golgi matrix
... such gene. A carboxy-terminal domain of the protein that is encoded by ORF At5 g66030 is 50% identical to the GRIP domain of Golgin-97. A fusion protein that consists of the this putative Arabidopsis GRIP domain and green fluorescent protein (GFP) was recently reported to colocate with the Golgi mar ...
... such gene. A carboxy-terminal domain of the protein that is encoded by ORF At5 g66030 is 50% identical to the GRIP domain of Golgin-97. A fusion protein that consists of the this putative Arabidopsis GRIP domain and green fluorescent protein (GFP) was recently reported to colocate with the Golgi mar ...
Physiological implications of the regulation of vacuolar
... restricted to endosomal function. Recently, Moulin and colleagues (37) examined renal biopsy specimens from patients with Dent’s disease and consistently noted inversion of H+-ATPase polarity in the proximal tubules to a basolateral distribution, in contrast to its apical localization in the normal ...
... restricted to endosomal function. Recently, Moulin and colleagues (37) examined renal biopsy specimens from patients with Dent’s disease and consistently noted inversion of H+-ATPase polarity in the proximal tubules to a basolateral distribution, in contrast to its apical localization in the normal ...
Structural Mechanisms for Regulation of Membrane
... Subfamily members tend to be functionally similar if not redundant (although differences in tissue and possibly subcellular distribution might be important), whereas group members typically have partially or completely distinguishable functions. Nevertheless, group members are more likely to functio ...
... Subfamily members tend to be functionally similar if not redundant (although differences in tissue and possibly subcellular distribution might be important), whereas group members typically have partially or completely distinguishable functions. Nevertheless, group members are more likely to functio ...
Increased Susceptibility of the Sickle Cell Membrane
... are subject to many changes due to the polymerization of hemoglobin S . Some of the membrane changes include: altered ion transport, altered cytoskeletal structure, phosphatidylserine transmembrane movement, and reduced enzyme function.‘ These changes appear to be associated with the formation of de ...
... are subject to many changes due to the polymerization of hemoglobin S . Some of the membrane changes include: altered ion transport, altered cytoskeletal structure, phosphatidylserine transmembrane movement, and reduced enzyme function.‘ These changes appear to be associated with the formation of de ...
The Pathogenic Fungus Paracoccidioides brasiliensis Exports
... Exosome-like vesicles containing virulence factors, enzymes, and antigens have recently been characterized in fungal pathogens, such as Cryptococcus neoformans and Histoplasma capsulatum. Here, we describe extracellular vesicles carrying highly immunogenic ␣-linked galactopyranosyl (␣-Gal) epitopes ...
... Exosome-like vesicles containing virulence factors, enzymes, and antigens have recently been characterized in fungal pathogens, such as Cryptococcus neoformans and Histoplasma capsulatum. Here, we describe extracellular vesicles carrying highly immunogenic ␣-linked galactopyranosyl (␣-Gal) epitopes ...
Ca2+ signalling and control of guard-cell volume in stomatal
... Figure 1 ABA leads to a concerted modulation (⊕, activation; O, inactivation) of three plasma membrane ion channels: the two dominant K+ currents (a) IK,in, (b) IK,out, and (c) the slowactivating anion current ICl (and, probably also (d) the H+-ATPase). ABA binds to an as yet unidentified receptor ( ...
... Figure 1 ABA leads to a concerted modulation (⊕, activation; O, inactivation) of three plasma membrane ion channels: the two dominant K+ currents (a) IK,in, (b) IK,out, and (c) the slowactivating anion current ICl (and, probably also (d) the H+-ATPase). ABA binds to an as yet unidentified receptor ( ...
Dual Location of the Mitochondrial Preprotein
... also detected the upper band of Tim17:23 complex, but not complex I (Figure 2B). To confirm further the dual locations of both Tim23-2 and B14.7, in vitro protein uptake assays of radiolabeled proteins were performed and analyzed by BN-PAGE. The uptake of radiolabeled Tim23-2 into the Tim17:23 comple ...
... also detected the upper band of Tim17:23 complex, but not complex I (Figure 2B). To confirm further the dual locations of both Tim23-2 and B14.7, in vitro protein uptake assays of radiolabeled proteins were performed and analyzed by BN-PAGE. The uptake of radiolabeled Tim23-2 into the Tim17:23 comple ...
SNARE (protein)
SNARE proteins (an acronym derived from ""SNAP (Soluble NSF Attachment Protein) REceptor"") are a large protein superfamily consisting of more than 60 members in yeast and mammalian cells. The primary role of SNARE proteins is to mediate vesicle fusion, that is, the fusion of vesicles with their target membrane bound compartments (such as a lysosome). The best studied SNAREs are those that mediate docking of synaptic vesicles with the presynaptic membrane in neurons. These SNAREs are the targets of the bacterial neurotoxins responsible for botulism and tetanus.