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DNA
DNA

... - Sugar & phosphate form backbone - The bases form the “steps” of ladder, held together by Hydrogen bonds • C-G = 3 hydrogen bonds • A-T = 2 hydrogen bonds ...
student worksheet
student worksheet

... a good description? Why or why not? In living things, the detailed directions for cells to make the proteins that control and compose the organism must be very precise. The code found in DNA is the basis for forming proteins. In this activity you will see how the proteins are formed through an amazi ...
Syllabus Checklist
Syllabus Checklist

... For a protein to be made or synthesised, the information has to be taken off the DNA molecule and used to link amino acids together in a specific sequence. This involves two processes—transcription and translation. Distinguish between transcription and translation by completing the table below. ...
THE CELLULAR AND MOLECULAR BASIS OF INHERITANCE
THE CELLULAR AND MOLECULAR BASIS OF INHERITANCE

... Adenine = Thymine (in DNA) Adenine = Uracil Cytosine =Guanine ...
13.3- The Human Genome
13.3- The Human Genome

...  2001- publication of a draft of the 3 billion base pairs (ATCG) of DNA in most human cells.  Completed in 2003. (13 year project) ...
Science 9 Chapter 4 Practice Test
Science 9 Chapter 4 Practice Test

... b. never happens since all mutations affect an individual. c. cannot be transmitted to the next generation. d. will not be seen until two or three generations have been produced. Substances or factors that cause mutations in DNA are called a. bacteria. c. mutagens. b. diseases. d. mutations. When a ...
Human Genetics WF, ML , SFdf
Human Genetics WF, ML , SFdf

... A Punnett spuare is a chart that shows the traits of the parents of an organism, wether they be dominant of recessive, and give the probability of that organisms gene code for that factor.  It will show if it will be dominat, recessive, if it has a hidden recessive, mixed hybrid or all of the above ...
Why the scientists want to extract the DNA from the cells? With the
Why the scientists want to extract the DNA from the cells? With the

... bank for Motor Neurone Disease (MND) , it made people know better about this disease, more and more DNA samples will let scientists identify the mutipul reasons cause this disorder, and in the not very far future, they might find the way to conquer it. Thirdly, the DNA samples can help with the exti ...
How Does DNA Determine the Traits of an Organism
How Does DNA Determine the Traits of an Organism

DNA TECHNOLOGY - Mount Mansfield Union High School
DNA TECHNOLOGY - Mount Mansfield Union High School

... bacteria that cause frost to form •Pesitcide resistant plants- When the farmer sprays his genetically modified crop with pesticides, he or she can destroy most of the pests without killing the plants ...
No Slide Title
No Slide Title

...  Recombinant DNA is introduced into appropriate host cell  Recombinant DNA is expressed at high levels in host cell Gene product may be purified for use in treatments (antibiotics, hormones, etc.) ...
Advanced Genetics Unit 2: DNA Structure and Processes Quiz Bowl
Advanced Genetics Unit 2: DNA Structure and Processes Quiz Bowl

... 21. It would seem that “protein-coding genes” only make up about _______% of the DNA in our cells? [1-2%] 22. RNA type responsible for shuttling amino acids to ribosomes during protein construction. [tRNA] 23. Name any type of regulatory RNA. [miRNA, siRNA, snRNA, snoRNA] 24. What does siRNA stand f ...
Answers questions chapter 12
Answers questions chapter 12

... end of the transposon. Once bound to the ends, the transposase brings them together to form a proteinDNA complex called the synaptic complex or transpososome. The transposase subunits then make double-strand cuts at both ends of the transposon, liberating the transposon and leaving a doublestranded ...
Document
Document

... • Cells can repaire some mistakes •Once introduced and not repaired, changes in DNA sequence are made permanent by DNA replication ...
Lecture
Lecture

...  1.045 billion bases sequenced  1800 microbial species estimated to exist in sample, ...
Slide 1
Slide 1

... Sequences of 3 bases in RNA code for a single amino acid There are 64 possible ‘triplets’ that can be formed from the 4 different bases, but there are only 20 amino acids (AA) In most cases, more than one type of triplet codes for a given AA For example, CAA and CAG both code for the same AA, glutam ...
Reading GuideBacterialGenetics(CH8)
Reading GuideBacterialGenetics(CH8)

... mutations, and how to select for mutants or use bacteria to study these mutations. We will focus on sections 8.1-8.5 for now. We will finish the last sections, 8.6-8.9, after talking about viruses. So let’s begin with a look at some key terms and the different types of mutations that can occur in ba ...
Genetic Engineering and The Human Genome
Genetic Engineering and The Human Genome

... DNA Cutting – restriction enzymes cut particular DNA sequences. Separating DNA – gel electrophoresis. Copy – using polymerase chain reaction “PCR” ...
Making Proteins
Making Proteins

PCR-assay of intragenic DNA lesions induced by ionizing radiation
PCR-assay of intragenic DNA lesions induced by ionizing radiation

... observed; To detect the intragenic distribution of different DNA alterations relative to the exon-intron structure of the gene under study ...
Directions for Dog Breed Genetics
Directions for Dog Breed Genetics

In situ - University of Evansville Faculty Web sites
In situ - University of Evansville Faculty Web sites

In situ - University of Evansville Faculty Web sites
In situ - University of Evansville Faculty Web sites

... – variable numbers of dinucleotide repeats – detected by PCR ...
Final Exam Review - Blue Valley Schools
Final Exam Review - Blue Valley Schools

... Dominant: a form of a gene (allele) that is expressed even if present with a contrasting recessive allele Recessive: a form of a gene (allele) that is only expressed when two copies are present ...
Gene Manipulation-2 - Workforce Solutions
Gene Manipulation-2 - Workforce Solutions

... Viruses are used • E. coli often used to express genes that have been transferred • Transformation is a common method for gene transfer ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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